• The Plant Pathology Journal
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• 제17권1호
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• pp.62-66
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• 2001

Detection of Xanthomonas axonopodis pv. citri (Xac) on citrus fruits for exporting is usually made by bacteriophage test (BPT) to demonstrate the pathogen-free status. BPT has rather time-consuming and complicate procedures for dealing with massive samples to be inspected. In this study, enzyme-linked immunosorbent assay (ELISA) was applied to detect Xac on fruits, and compared with BPT. In ELISA, positive reactions occurred in the bacterial densities of $3\times10^5$ cells/ml or more. To detect the bacterial infection on citrus fruits with a density of lower than $3\times10^5$ cells/ml, the bacterial suspensions were mixed with fruit rinse water and incubated in broth medium. Ordinary peptone sucrose broth (PSB) was not a proper medium for increasing Xac density specifically enough to be detect by ELISA. On the other hand, modified PSB (MPSP) amended with Fe-EDTA (0.25 g/$\ell$) and 2.5% potato-dextrose broth sufficed to differentiate uninfected and infected citrus fruits by ELISA after 24 h incubation of the fruit rinse water. Using various citrus samples from infected and uninfected fields, efficiencies in detecting Xac on fruits were compared between ELISA and BPT. For infected fruits samples, ELISA detected Xac by 100%, while BPT by about 44%, indicating that the detection efficiency was improved by 23.5% by ELISA, compared to BPT. In addition, ELISA has simpler procedures for testing and is less time-consuming than BPT, suggesting that ELISA may be accurate and simple method to detect Xac on citrus fruits.

• Journal of Medicine and Life Science
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• 제15권1호
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• pp.6-11
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• 2018

Previous studies have suggested that Citrus fruits might suppress the proliferation of various cancer cells. However, little is known about any specific ingredients in the extract of Citrus fruits to exert its anti-proliferative activity in cancer cells. The present study aimed to identify the active ingredients in Citrus fruits to suppress the proliferation of rat hepatocellular carcinoma cells. Among tested compounds, two polymethoxylated flavones (nobiletin and tangeritin) showed significant anti-proliferative activity whereas other compounds (synephrine, rutin, hesperidin) did not. Interestingly, nobiletin as well as tangeritin also decreased the protein amount of gluconeogenic enzymes, PEPCK and G6Pase. The possible involvement of gluconeogenic activity in the proliferation of hepatocellulacarcinoma cells are further to be investigated.

• Journal of Applied Biological Chemistry
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• 제44권3호
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• pp.143-146
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• 2001

Eight flavonoids, including rutin, naringin, hesperidin, quercetin, hesperetin, nobiletin, 3,5,6,7,8,3',4'-methoxylated flavone, and tangeretin, in the peels of satsuma mandarin (Citrus unshiu) species of Halla, Gungcheon, Hungjin, Namgam-20, Illnam-1, and Chungdo harvested between August and December were analyzed through HPLC. Hesperidin content of Halla harvested during early maturation was 28.70 mg/g, and was the highest among the tested citrus fruits. Rutin content of Hungjin harvested during early maturation was 2.66 mg/g. Naringin in all citrus species and hesperetin in Halla, Gungchun, Namgam-20, and Chungdo were only detected in the peel of fruits harvested during early maturation. Hesperidin and rutin were detected mainly in all citrus species, and other flavonoids in trace. Flavonoid content in the peel of fruits was high during early maturation. Flavonoid contents in the peels of all fruit samples were generally high in the early stage of maturation, which then decreased rapidly.

• 한국식품저장유통학회지
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• 제2권2호
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• pp.251-257
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• 1995

In order to determine the quality of Citrus unshiu(medium cultivar of satsuma mandarin) produced in Cheju, citrus fruits sampled at sorting places and harvested directly on citrus tree in south and north area of Cheju were analyzed. The fruits were grown in size till early of November, and soluble solids were increased continuously after that. Compared with the quality of citrus fruits as a factor of soluble solids, firmness, total sugar, pH, and color index, the optimum harvest periods were supposed to be reasonable from early of December for C. unshiu Marc. var. yonezawa and C. unshiu Marc. var. hayashi, Fruit weights and peel thickness had a linear correlation with increasing fruit size, but soluble solids and acid contents had not a correlation. The selection of C. unshiu variety was needed to determine by the properties of storage.

• 한국식품저장유통학회지
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• 제4권2호
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• pp.131-137
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• 1997

Effect of storage on the tree and quality of early cultivar of Citrus unshiu Marc. produced in Cheju according to harvest time were investigated. Soluble solids, acid content, flesh ratio, firmness and specific cavity of the fruits harvested at 27th of November were 12.0, 1.03%, 79.98%, 0.774kg-force, 0.912, respectively. Soluble solids, total sugar and reducing sugar were increased gradually, but acid content was decreased slightly as delayed in harvest time. Otherwise specific gravity and firmness were not shown in great during harvest time, color index(a/b) was increased sharply till late November and then remained in stationary phase. On these result, we recommend that the harvest time of early cultivar of Citrus unshiu Marc. would be during December because of wilting partly. Fully ripened-fruits harvested late, compared to ordinary harvest time, with cold storage would be kept more quality and freshness of fruits for fresh consumption than the fruits harvested early and stored at room temperature.

• Applied Biological Chemistry
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• 제40권2호
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• pp.117-122
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• 1997

제주산 궁천조생의 저장조건에 따른 저온저장 특성을 검토하였다. 상온저장에 비해 저온저장 감귤은 저장기간 중 가용성 고형물, 총당, 비타민 C의 변화가 매우 적었으며, 부패율과 중량감소가 각각 10% 이내 수준에서 비교적 신선도를 유지한 상태에서 3월 하순까지 저장이 가능한 것으로 보였다. 또한, 중량감소, 경도의 저하, 당함량의 감소는 저장기간 중에 계속하여 서서히 일어났다. 필름포장과 wax-coating은 중량감소와 외관향상에는 도움을 주었으나 장기간 저장에는 부패율이 증가하였다. 습도조절을 하지 않은 저온저장은 습도조절한 경우와 비교하였을 때 부패율과 중량감소가 컸다. 증산작용으로 인한 껍질수분의 감소에 따라 과육으로부터 수분이동이 발생하여 저장기간 중 과육율이 감소하였으며, 저장 60일 후부터 경도저하가 많이 일어나 조직이 연화됨을 알 수 있었다. 조기수확한 감귤에 비하여 완숙한 감귤이 부패율과 중량감소가 적어 저장 100일간을 기준하여 출하하는 것이 바람직하였다. 상온저장한 감귤에 비해 저온저장한 감귤은 저장 4개월 이후 출고하였을 경우 외관 및 맛에 비교적 우수하였으며, 출고후 필름포장한 감귤의 부패율은 급속히 증가하여 취급에 주의가 필요하였다. 조생온주의 경우 저온에서 장기간 저장이 어려움을 알 수 있어서 저장용 감귤의 특성을 고려하여 저장조건을 설정할 필요가 있었다.

• The Plant Pathology Journal
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• 제36권1호
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• pp.106-110
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• 2020

Citrus mosaic sadwavirus (CiMV) is a closely related virus with the Satsuma dwarf virus (SDV) along with Navel orange infectious mottling virus (NIMV), Natsudaidai dwarf virus (NDV), and Hyugagatsu virus (HV). The present study found that the typical symptoms of CiMV-infected citrus fruits include the appearance of dark blue speckles or ringspots on fruit rinds and the browning of oil glands in the spots as rind coloring began. As rind coloring progressed, the spots gradually faded, whereas the browning of the oil glands worsened to the point that the tissues surrounding the oil glands became necrotic. In very early satsuma mandarins (Citrus unshiu 'Miyamoto Wase') and 'Setoka' cultivar (C. hybrid 'Setoka') of late-maturity citrus, the symptomatic fruits were eventually dropped. And in early satsuma mandarin (C. unshiu 'Miyakawa Wase'), the peel hardness of the virus-infected fruit (1,618.3 ± 305.5, g-force) was more than twice as hard as that of the healthy fruit (636.5 ± 39.1, g-force). The ratio of flesh weight to total fruit weight was higher for the healthy fruit (77.3 ± 1.7%) than for the infected fruit (70.7 ± 0.6) and peel puffing was more severe in the infected fruit (2.9 ± 0.4 mm) than in the healthy fruit (0.9 ± 0.2 mm). The soluble solids content in infected citrus fruits was less values than the healthy fruit by 0.5-1.5 °Brix. These findings reveal that CiMV infection on citrus trees reduces the fruit quality of citrus.

• Applied Biological Chemistry
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• 제41권2호
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• pp.141-146
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• 1998

조생온주의 적정 수확시기를 결정하기 위하여 선과장에서 수집한 시료와 감귤나무에서 시기별로 직접 수확한 시료의 이화학적 품질을 분석하였다. 궁천조생과 흥진조생 모두 10월 중순까지 감귤의 비대가 계속 일어났으며, 이후에도 당도는 계속하여 증가하였다. 나무에 달려 있는 위치에 따라서도 감귤의 크기가 다른 경향을 보였으며, 남쪽 방향의 중간 위치에 달려 있는 감귤이 품질면에서 비교적 우수한 것으로 나타났다. 감귤의 품질에 관여하는 가용성고형물, 경도, 총당, pH, 착색도 등을 종합적으로 볼 때 흥진조생의 경우 11월 하순에, 그리고 궁천조생의 경우 12월 초순에 수확하는 것이 품질면에서 좋을 것으로 보였다. 선과장에서 수집한 시료는 농가에서 관행수확시에 수확한 다음 상온저장 후에 시기별로 출하하기 때문에 정확한 수확시기를 결정하는데는 미흡한 것으로 여겨졌다.

• Agribusiness and Information Management
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• 제7권1호
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• pp.1-9
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• 2015

The purpose of this study is to develop a growth prediction model that can predict growth and development information influencing the production of citrus fruits: the growth model algorithm that can predict floral leaf ratio, number of fruit sets, fruit width, and overweight depending on the main period of growth and development with consideration of the applied weather factors. Every year, large scale of manpower was mobilized to investigate the production of outdoor-grown citrus fruits, but it was limited to recycling the data without an observation supporting system to systemize the database. This study intends to create a systematical database based on the basic data obtained through the observation supporting system in application of an algorithm according to the accumulated long term data and prepare a base for its continuous improvement and development. The importance of the observed data is increasingly recognized every year, and the citrus fruit observation supporting system is important for utilizing an effective policy and decision making according to various applications and analysis results through an interconnection and an integration of the investigated statistical data. The citrus fruit is a representative crop having a great ripple effect in Jeju agriculture. An early prediction of the growth and development information influencing the production of citrus fruits may be helpful for decision making in supply and demand control of agricultural products.

• 한국식품저장유통학회지
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• 제7권1호
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• pp.51-56
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• 2000

Quality characteristics of Satsuma mandarin (Citrus unshiu Marc.var.miyagawa) harvest on early December according to production areas and sea level altitude of Cheju were investigated. Soluble solids and flesh ratio were distributed 9.32∼12.50, 75.95%-81.3 in citrus fruits harvested in orchards located below 10m sea level altitude (lower area), and was 10.23-12.17 , 77.35%-80.29% on those over 150m sea level altitude )higher area), respectively. Acid content of citrus juice was 0.95%-1.26% in fruits harvested inlower area, and was 1.02%-1.39% in higher area. In proportion to late harvesting , Brix/acid ratio and fruits quality was improved. Soluble solids of fruits harvested in higher area were higher than those of fruits from higher area, but acid contents were on the contrary . In sensory evaluation on fruit appearance, the differences between two altitudes were not clear.