• BMB Reports
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• v.37 no.3
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• pp.304-313
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• 2004

Three-dimensional (3D) models for the 65-kDa activated Cry4A and Cry4B $\delta$-endotoxins from Bacillus thuringiensis subsp. israelensis that are specifically toxic to mosquito-larvae were constructed by homology modeling, based on atomic coordinates of the Cry1Aa and Cry3Aa crystal structures. They were structurally similar to the known structures, both derived 3D models displayed a three-domain organization: the N-terminal domain (I) is a seven-helix bundle, while the middle and C-terminal domains are primarily comprise of anti-parallel $\beta$-sheets. Circular dichroism spectroscopy confirmed the secondary structural contents of the two homology-based Cry4 structures. A structural analysis of both Cry4 models revealed the following: (a) Residues Arg-235 and Arg-203 are located in the interhelical 5/6 loop within the domain I of Cry4A and Cry4B, respectively. Both are solvent exposed. This suggests that they are susceptible to tryptic cleavage. (b) The unique disulphide bond, together with a proline-rich region within the long loop connecting ${\alpha}4$ and ${\alpha}5$ of Cry4A, were identified. This implies their functional significance for membrane insertion. (c) Significant structural differences between both models were found within domain II that may reflect their different activity spectra. Structural insights from this molecular modeling study would therefore increase our understanding of the mechanic aspects of these two closely related mosquito-larvicidal proteins.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.24 no.4
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• pp.466-473
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• 2013

In this paper, a compact planar multi-loop self-resonant coil of high quality factor with a coaxial cross section is proposed for effective wireless charging. The proposed coil has high Q-factor and a resonant frequency of a coil can be easily controlled by adjusting distributed capacitance. For designing the coil, a self-inductance and a distributed capacitance are calculated theoretically. The self-inductance is calculated from the sum of the mutual energies between small circular loops that are made by dividing the cross section of the coil. To verify its properties and calculation results, the self-resonant coils are fabricated by using a coaxial cable with characteristic impedance of $50{\Omega}$. The measured frequencies are very consistent with the calculated ones. In addition, the resonant frequency can be adjusted slightly by the tuning parameter ${\gamma}$. The resonant coils are applied to a tablet PC, the Q-factors of the Tx and Rx resonant coils are 282 and 135, respectively. As a result of measurement when height between the two resonant coils is 4.4 cm, the power transfer efficiency is more than 80 % within a radius of 5 cm.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.149-153
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• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.23 no.5
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• pp.613-623
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• 2012

In this paper, we present an ultra miniaturized voltage tuned oscillator, with HMIC-type amplifier and phase shifter, using LTCC artificial dielectric resonator. ADR which consists of periodic conductor patterns and stacked layers has a smaller size than a dielectric resonator. The design specification of ADR is obtained from the design goal of oscillator. The structure of the ADR with a stacked circular disk type is chosen. The resonance characteristic, physical dimension and stack number are analyzed. For miniaturization of ADRO, the ADR is internally implemented at the upper part of the LTCC substrate and the other circuits, which are amplifier and phase shifter are integrated at the bottom side respectively. The fabricated ADRO has ultra small size of $13{\times}13{\times}3mm^3$ and is a SMT type. The designed ADRO satisfies the open-loop oscillation condition at the design frequency. As a results, the oscillation frequency range is 2.025~2.108 GHz at a tuning voltage of 0~5 V. The phase noise is $-109{\pm}4$ dBc/Hz at 100 kHz offset frequency and the power is $6.8{\pm}0.2$ dBm. The power frequency tuning normalized figure of merit is -30.88 dB.

• Journal of Korea Spatial Information System Society
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• v.9 no.1
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• pp.79-90
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• 2007

In this paper we proposed and implemented an incident detection model which combines fuzzy algorithm and traffic pattern in order to enhance the efficiency of incident detection for the highways with lamps. Most of the existing algorithms dealt with highways without lamps and can not be used for detecting incidents in the highways with lamps. The data used for model building are traffic volume, occupancy, and speed data. They have been collected by a loop sensor at 5 minutes interval at a point in the Internal Circular Highway of Seoul for the period of 3 months. In this model, the three parameters collected by sensor were fuzzified and combined with the daily traffic pattern of the link. The test of efficiency of the propsed model was performed by comparing the result of proposed model with traditional APID algorithm and fuzzy algorithm without the pattern data respectively. The result showed significant amount of improvement in reducing the false incident detection rate by 18%.

• Journal of Energy Engineering
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• v.19 no.4
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• pp.234-239
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• 2010

Heat transfer characteristics were experimentally investigated for ice slurry which was made from 6.5% ethylene glycol-water solution flowing in the circular pipe. The test section was made of a copper tube of 13.84 mm inner diameter and 1,500 mm length. The ice slurry was heated by passing hot water through an annulus surrounding the test section. The ice packing factor(IPF) and the mass flux of the experiments were varied from 0 to 25% and from 1,000 to 3,000 kg/$m^2s$ respectively at a fixed hot water temperature and flow rate. The measured heat transfer rates increase with the mass flow rate and IPF; however the effect of IPF appears to be minor at high mass flow rate. At the low mass flow rate condition, a sharp increases in the heat transfer coefficient was observed when the IPF was above 15 ~ 20%. And finally the measured heat transfer coefficients were compared with those calculated from the correlations.

• Progress in Superconductivity
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• v.8 no.2
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• pp.164-168
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• 2007

We developed a SQUID magnetometer based on Double Relaxation Oscillation SQUID(DROS) for measuring magnetocardiography(MCG). Since DROS provides a 10 times larger flux-to-voltage transfer coefficient than the conventional DC-SQUID, simple flux-locked loop electronics could be used for SQUID operation. Especially, we adopted an external feedback to eliminate the magnetic coupling with adjacent channels. When the DROS magnetometer was operated inside a magnetically shielded room, average magnetic field noise was about 5 $fT/^{\surd}Hz$ at 100 Hz. Using the DROS magnetometer, we constructed a multichannel MCG system. The system consisted of 61 magnetometers are arranged in a hexagonal structure and measures a vertical magnetic-field component to the chest surface. The distance between adjacent channels is 26 mm and the magnetometers cover a circular area with a diameter of 208 mm. We recorded the MCG signals with this system and confirmed the magnetic field distribution and the myocardinal current distribution.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.6
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• pp.99-110
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• 2005

These days, many image processing techniques have been studied for effective image compression. Among those, The 2D image filtering is widely used for 2D image processing. The 2D image filtering can be implemented by performing the 1D linear filter separately in the horizontal and vertical direction. Efficiency of image compression depends on what filtering method is used. Generally, circular convolution is widely used in 2D image filtering for image processing. However it doesn't consider correlations at the boundary region of image, therefore effective filtering can not be performed. To solve this problem. I proposed new convolution technique using loop convolution which satisfies the 'alias-free' and 'error-free' requirement in the reconstructed image. This method could provide more effective compression performance than former methods because it used highly-correlated data when performed at the boundary region. In this paper, Sub-band Coding(SBC) was adopted to analyze efficiency of proposed filtering technique, and the simulator developed by Java-based language was used to examine the performance of proposed method.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.2
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• pp.229-234
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• 2006

Acquisition is to detect the presence of the GPS signal. Once the signal is detected, the estimated frequency and code phase are passed to a tracking loop to demodulate the navigation data. In order to detect the weak signal, multiple length of data integration is always needed. In this paper, we present five different acquisition approaches based on circular correlation and Fast Fourier Transform (FFT), using coherent as well as non-coherent integration techniques for the multiple length of collected GPS satellite signal. Moreover a general approach of determining the acquisition threshold is introduced based on noise distribution which has been proved effective, and independent of the hardware. In the end of this paper, the processing speed and acquisition gain of each method are illustrated, compared, and analyzed. The results show that coherent approach is much more time consuming compared to noncoherent approaches, and in the case of multiple length of data integration from 2ms to 8ms, the processing times consumed by the fastest non-coherent acquisition method are only 25.87% to 1.52% in a single search, and 34.76% to 1.06% in a global search of those in the coherent acquisition. However, coherent acquisition also demonstrates its better performance in the acquisition gain, and in the case of 8ms of data integration it is 4.23 to 4.41 dB higher than that in the non-coherent approaches. Finally, an applicable scheme of combining coherent and non-coherent acquisition approaches in the development of a real-time Software GPS receiver in the University of Tokyo is provided.

• Journal of the Korean Society of Earth Science Education
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• v.9 no.2
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• pp.186-198
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• 2016

The purpose of this study is to identify the composition elements and processes of the rock cycle that pre-service teachers in earth science field understand. Second-year students majoring in earth science were selected as research objects, all of whom attended teacher's university located in the southern region. The methods of arranging the composition elements of the rock cycle in a structured way and displaying the cycle direction through the arrow and describing the cycle processes in detail were applied. Pre-service teachers recognized 5 composition elements: magma, igneous rocks, sediments, sedimentary rocks, metamorphic rocks of the rock cycle and cycle processes was represented into 20 branches. The cycle type was analyzed by four criteria: linear, triangular, circular, complex, some pre-teachers did not complete the loop of the cycle or some showed incomplete understanding of the cycle structure simply displayed in one direction. The rock cycle is an important theme of the earth system education characterized by dynamism and complexity.