• Journal of the Korean Society of Tobacco Science
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• v.1 no.2
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• pp.93-102
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• 1979

Chemical compositions of Flue - cured and Burley tobacco leaf and smoke were analyzed to find out simple correlation between compositions. Based on the simple correlation ( $r$ ) data, four regression equations were obtained for dry condensate and nicotine in smoke as follows, $Y_{FT}=\;0.816+4.638X_1+0.346X_2$......................................(1) $Y_{BT}=\;0.235+0.884X_3-0.088X_4$......................................(2) $Y_{FN}=\;0.015+0.090X_5+0.476X_1$......................................(3) $Y_{BN}=-0.026+0.101X_3-0.006X_4$.........................................(4) where, $Y_{FT}$ : dry condensate (mg/cig.) in cigarette smoke of Flue-cured $Y_{BT}$ : dry condensate (mg /cig. ) in cigarette smoke of Burley $Y_{FN}$ : nicotine contents (mg/cig.)in cigarette smoke of Flue-cured $Y_{BN}$ : nicotine contorts (mg/cig. ) in cigarette smoke of Burley $X_1$ : total alkaloid contents (%) of Flue-cured $X_2$ : curde ash contents (%)of Flue -cured $X_3$ : alcohol -benzene extract contents (%) of Burley $X_4$ : curde ash contents (%) of Burley $X_5$ : hexane extract content (%) of Flue -cured Standard deviation for regressions equations were obtained respectively. equation(1),$\pm$3.16 : equation (2),$\pm$1.94 : equation (3),$\pm$0.35 , equation (4) $\pm$ 0.33. Regression equations between dry condensate and nicotine contorts in cigarette smoke were also obtained in the forms of equation(5) and(6) $Y_{FN}$= 0.274 $Y_{FT}$- 3.259............................(5) $Y_{BN}$= 0.249 $Y_{BT}$ - 2.093.............................(6) Standard deviations, $\pm$ 0.061 and $\pm$ 0.152, were obtained for regression equation(5) and (6) respectively.

• Journal of the Korean Society of Tobacco Science
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• v.25 no.2
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• pp.128-136
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• 2003

Although tobacco smoke has been known to have genotoxicity as well as cytotoxicity, the sensitivity of the cell lines used against cigarette smoke is poorly understood. The objective of this study was to evaluate and compare the genotoxicity of several cell lines, which are routinely used in the in vitro assays, with cigarette smoke condensate(CSC) of Kentucky Reference Cigarette 1R4F. In the micronucleus(MN) induction assays, murine(CHO-K1, V79, BALB/c 3T3) cell lines and human(MCF-7, A549) ones were used. As a result, the CSC exhibited cytotoxicity with a concentration-dependent response in all cell lines. EC$_{50}$ of CSC in CHO-K1, V79, BALB/c 3T3, MCF-7 and A549 were 140, 125, 100, 116 and 109 $\mu\textrm{g}$/mL, respectively. On the other hand, the spontaneous micronucleated cell(MNC) frequency was stable and reproducible in every cell lines tested in this study. The dose-response of various cell lines to the induction of MN by CSC was estimated using linear regression analysis. CSC(0～100 $\mu\textrm{g}$/mL) caused a dose-dependent MN induction in CHO-K1, V79, BALB/c 3T3 and MCF-7 cell lines. Putting together all the data obtained and linear regression analysis of the data, we concluded that V79 cells are more susceptible to the accurate assessment of CSC-induced MN than the others.s.

• Biomolecules & Therapeutics
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• v.12 no.3
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• pp.185-188
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• 2004

The purpose of this study is to evaluate the effect of cigarette smoke condensate (CSC) on toxification/detoxification metabolic pathway in primary cultured rat hepatocytes. We measured the activities of cytochrome P450 monooxygenases (CYP450s) and UDP-glucuronyltransferase, sulfotransferase and glutathione-S-transferase in CSC-treated rat hepatocytes. CSC significantly increased the activities of hepatic CYP4501A1 and CYP4501A2 to 7.5 fold and 1.6 fold respectively, compared with control level. However, CSC did not affect the activities of conjugation enzymes. We a1so examined if treatment of CSC could change thc cytotoxicity of acetaminophen (AA) through modulation of metabolizing enzymes. In rat hepatocytes, pretreatment with CSC potentiated the cytotoxicity of AA. This result indicates that potentiation of AA toxicity by CSC pretreatment may be related to induction of CYP4501A1 and CYP4501A2.

• Journal of the Korean Society of Tobacco Science
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• v.27 no.1 s.53
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• pp.51-58
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• 2005

The objective of this study was to evaluate the effect of cigarette filter on in vitro cytotoxicity of cigarette mainstream smoke from the cigarette. In this work, we used 3 types of cigarettes included non-filtered 2R4F cigarette, cellulose acetate-filtered 2R4F cigarette, and carbon dual-filtered 2R4F cigarette which was made from original 2R4F by replacing with an acetate filter containing carbon. The cytotoxicity of both the cigarette smoke condensate (CSC), which was collected in Cambridge filter pad, and the gas/vapor phase (GVP), which was bubbled through in phosphate-buffered saline in a gas-washing bottle, was determined using a neutral red uptake assay with CHO-K1 cells. With regard to cytotoxicity when calculated on an equal puff basis, the cytotoxicity of CSC from the filtered cigarettes was lower than that of the non filtered cigarette. Also, $EC_{50}$ vlaue of GVP from carbon filter cigarette was 40.9 puff/L, indicating the cytotoxicity to be $20\%$ lower than that of the CA filter cigarette. The cytotoxicity of the GVP was correlated to the several vapor phase components (formaldehyde, acetaldehyde, acetone, acrolein, crotonaldehyde and MEK). In conclusion, carbon filter, which significantly reduced the amount of carbonyl compounds in mainstream cigarette smoke, results in significant reductions in the cytotoxicity potential of the smoke.

• Environmental Analysis Health and Toxicology
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• v.30
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• pp.14.1-14.7
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• 2015

Objectives Cigarette smoking is associated with carcinogenesis owing to the mutagenic and genotoxic effects of cigarette smoke. The aim of this study was to evaluate the mutagenic and genotoxic effects of Korean cigarettes using in vitro assays. Methods We selected 2 types of cigarettes (TL and TW) as benchmark Korean cigarettes for this study, because they represent the greatest level of nicotine and tar contents among Korean cigarettes. Mutagenic potency was expressed as the number of revertants per ${\mu}g$ of cigarette smoke condensate (CSC) total particulate matter whereas genotoxic potency was expressed as a concentration-dependent induction factor. The CSC was prepared by the International Organization for Standardization 3308 smoking method. CHO-K1 cells were used in vitro micronucleus (MNvit) and comet assays. Two strains of Salmonella typhimurium (Salmonella enterica subsp. enterica ; TA98 and TA1537) were employed in Ames tests. Results All CSCs showed mutagenicity in the TA98 and TA1537 strains. In addition, DNA damage and micronuclei formation were observed in the comet and MNvit assays owing to CSC exposure. The CSC from the 3R4F Kentucky reference (3R4F) cigarette produced the most severe mutagenic and genotoxic potencies, followed by the CSC from the TL cigarette, whereas the CSC from the TW cigarette produced the least severe mutagenic and genotoxic potencies. Conclusions The results of this study suggest that the mutagenic and genotoxic potencies of the TL and TW cigarettes were weaker than those of the 3R4F cigarette. Further study on standardized concepts of toxic equivalents for cigarettes needs to be conducted for more extensive use of in vitro tests.

• YAKHAK HOEJI
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• v.55 no.3
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• pp.251-259
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• 2011

Cigarette smoke condensate (CSC) is known to be carcinogenic compound. CSC contains many organic compounds such as polycyclic aromatic hydrocarbons (PAHs), and heterocyclic amine compounds (HCAs). Reactive oxygen species (ROS) are also generated and induce oxidative DNA damage during the metabolism of CSC. The rat microsome mediated and DNA repair enzyme treated comet assays together with conventional comet assay were performed to evaluate the mechanisms of CSC genotoxicity. The organic extract of CSC induced oxidative and microsome mediated DNA damage. Vitamin C as a model antioxidant reduced DNA damage in endonuclease III treated comet assay. One of flavonoid, galangin as a CYP1A1 inhibitor, reduced DNA damage in the presence of S-9 mixture. The ethanol extracts of the mixed vegetables (BV) or the mixed fruits (BF) showed potent inhibitory effects against CSC induced DNA damage with oxidative DNA lesions and in the prescence of S-9 mixture. These results indicate that BV and BF could prevent CSC-induced cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 in mammalian cells.

• Journal of the Korean Society of Tobacco Science
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• v.12 no.1
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• pp.3-7
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• 1990

Cigarette smoke is an aerosol consisted of both vapor and Particulate phases. In this study, the smoke condensates from several blended cigarettes were dissolved in water and the pH of the resulting solution was determined. The pH of the smoke condensate decreased with increase of puff number. In the beginning the pH decreased a little, while decreased remarkably from puff No.5 to puff No.10.

• Journal of the Korean Society of Tobacco Science
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• v.10 no.1
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• pp.49-57
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• 1988

The phenolic compounds were isolated from the cigarette smoke condensate using two immiscible organic solvents by a partition coefficient. Of them, 20 phenolic compounds were identified by GC and GC/MS. The amount of the phenolic compounds was phenol, p-crestol, hydroxybensoic acid in order. And there was no difference in composition of these compound dependent on leaf tobacco . About 70% amount of these compounds were absorbed to cellulose acetate filter used. The contents and composition of these compounds were various in the commercial cigarette.

• Archives of Pharmacal Research
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• v.5 no.2
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• pp.71-77
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• 1982

Improvements in the analytical methodology used in the gas chromatographic/mass spectral analysis of phenolic compounds from cigarette smoke are described. For the direct analysis of crude samples, pyridine extraction and the glass capillary column GC was used for the separation of phenolics as trimethylsilyl derivativatives. The separations of cigarette smoke on Carbowax 20M and SE-54 wall coated open tubular columns are given. Improved methodology for the routine quantitation of the identified components using the computer-controlled multiple ion selection technique of MS presented. Considerations pertaining to routine analyses of a multitude of complex smoke samples are also discussed.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.1
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• pp.67-72
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• 1983

A gas chromatographic procedure is described which permits seperation and determination of nonvolatile organic acids and higher fatty acids simultaneously in tobacco leaves and cigarette smoke condensate. The transfer rates from cigarettes to main stream smoke were examined for eight of the nonblending cigarettes. Average transfer rates for three varieties tobacco leaves, respectively were linolenic 31 and 62% linoleic 21 and 59% palmitic 29 and 38% malic 4 and 6%.