• Korean Journal of Veterinary Research
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• v.39 no.5
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• pp.908-920
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• 1999

We investigated the cytogenetic characteristics of Korean native goat(Capra hircus). Chromosome slides were prepared from peripheral blood cell cultures. GTG, GBG, RBG and CBG-banding techniques were employed on those slides. The high resolution karyotype of Korean native goat could be made with the incorporation of BrdU. Korean native goat has 60 chromosomes composed of 58 autosomes and XY or XX sex chromosomes. All of autosomes of Korean native goat were acrocentric chromosomes. X chromosome was submetacentric and Y chromosome was metacentric. The GTG, GBG and RBG-band patterns of Korean native goat were similar to those of other goats. CBG-band regions were distinct at the proximal portion of the long arms of all autosomes in Korean native goats. According to our investigation, there was no significant difference in chromosomal band patterns between Korean native goat and other goats. It might be necessary to use molecular genetic markers for clarifying the genetical characteristics of Korean native goat whose biological characteristics are not clearly defined.

• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.311-315
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• 2003

This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macrocephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A. japonica and A. macrocephala were 2n=24. The length. of the mitotic metaphase chromosomes of A. japonica ranged from $0.70\;to\;1.60{\mu}m$ with a total length. of $12.11{\mu}m$ and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A. macrocephala ranged from $0.90\;to\;2.35{\mu}m$ with a total length of $16.58{\mu}m$ and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A. japonica chromosomes was shorter than that of A. macrocephala, but A. japonica had one subtelocentrics (chromosomes 4) different from A. macrocepha1a. chromosomes. The F1SH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A. macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.

• Journal of Biomedical Engineering Research
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• v.17 no.4
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• pp.545-552
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• 1996

Researches on chromosome are very significant in cytogenetics since a gene of the chromosome controls revelation of the inheritance plasma The human chromosome analysis is widely used to diagnose genetic disease and various congenital anomalies. Many researches on automated chromosome karyotype analysis has been carried out, some of which produced commercial systems. However, there still remains much room for improving the accuracy of chromosome classification. In this paper, we propose an algorithm for reconstruction of the chromosDme image to improve the chromosome classification accuracy. Morphological feature parameters are extracted from the reconstructed chromosome images. The reconstruction method from chromosome image is the 32 direction line algorithm. We extract three morphological feature parameters, centromeric index(C.I.), relative length ratio(R.L.), and relative area ratio(R.A.), by preprocessing ten human chromosDme images. The experimental results show that proposed algorithm is better than that of other researchers'comparing by feature parameter errors.

• The Korean Journal of Zoology
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• v.7 no.1
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• pp.29-32
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• 1964

A study on chromosome of leucocytes in blood cultures derived from 6 normal Korean was performed . Exact chromosome counts were carried out on 205 cells in male, 211 in female , of which 86.05% revealed a chormosome mordal number of 46. On the basis of relative chromosome lengths and position of centromeres, the Karyotype that the human chromosomes were classified into 7 groups with 22 airs of autosome and one pair of sex chromosome was determined accoridng to the method of denver report. The chromosome number on metaphase was observed in short term cultures of leucocytes from the peripheral blood of 2 patients with chronic granulocytic leukemia and 1 patient with acute granulocitic leukemia . and the chromosome morpholoogy was also investigated in one acute leukemic patient. In all leukemic cases the leucocytes showed the constant value of 46 in the stem -line of chormosome number. But the frequency of cells with 46 chromosomes appeared in the 3 cases was 67.30% in average with a slightly higher range in hypo-andhyper-diploid chromosome numbers than in normal human, The idiogram analysis did not show any abnormality of chromosome in acute leukemic cells.

• Korean Journal of Ichthyology
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• v.30 no.2
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• pp.71-74
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• 2018

The karyotype analysis of an endangered freshwater fish, Microphysogobio koreensis, was performed to obtain basic data for phylogenetic information. To carry out our study, 4 specimens were collected in Seomjingang River and Nakdongang River and its kidney was treated by flame-drying method. The chromosome number of this species demonstrated 50 diploid chromosomes, with two populations of M. koreensis not significantly different. The karyotype revealed 2n=26m+24sm, consisting of 26 metacentric (m) and 24 submetacentric (sm) chromosomes with the total fundamental arm number determined as FN=100. Total arm length and arm ratio of the chromosomes were $1.44{\sim}2.68{\mu}m$ and 1.27~2.27, respectively. The karyotype of M. koreensis was first reported in this study.

• The Korean Journal of Zoology
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• v.34 no.1
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• pp.54-58
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• 1991

감돌고기속 어류에는 2종이 알려져 있으며 이들은 모두 한국고유어종이다. 감돌고기 Pseudopugtungia nigra의 핵형분석 결과 diploid chromosome number는 50이었으며, 7쌍의 metacentric, 18쌍의 $_{submeta}$telocentric chromosome으로 구성되어져 잇었다. 가는 돌고기 P. tenuicorpus의 2N은 50이었으며 10쌍의 metacentric, 15쌍의 $_{submeta}$telocentric chromosome으로 구성되어져 있었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.25-25
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• 2019

Chromosome numbers and karyotypes in flowering plants have been considered to be prominent features in taxonomic and evolutionary context. Despite the increasing numbers of cytological studies in Asteraceae, karyotype analysis of Cirsium Mill. and Carddus L. in Korean population have not been performed carefully. In this study, the chromosome numbers and karyotype analysis of all eight species of the genus Cirsium Mill. and one species of Carddus L. were analyzed. While the chromosome number in Carduus crispus L. was diploid (2n = 2x = 18 or 18+2Bs) with x = 9 as the base chromosome number, all seven species of Cirsium were diploid with x = 17 except for Cirsium lineare (Thunb.) Sch. Bip. (x = 14). The chromosome number in C. pendulum Fisch. ex DC. presented 2n = 2x = 34 from two populations and C. lineare exhibited 2n = 2x = 28 from one population. Aneuploidy was occasionally found in C. japonicum Fisch. ex DC. var. spinossinum Kitam. (2n = 2x = 34, 35, 36), C. rhinoceros (H. $L{\acute{e}}v.$ & Vaniot) Nakai (2n = 2x = 32, 34), C. setidens (Dunn) Nakai (2n = 2x = 30, 31, 32) and C. vlassovianum Fisch. ex DC. (2n = 2x = 31, 32). While Cirsium japonicum Fisch. ex DC. var. japonicum possessed several B-chromosomes (2n = 2x = 34, 35, 36), polyploidy was only encountered in Cirsium nipponicum (Maxim.) Makino. (2n = 4x = 68) from two populations in Ulleung Island. The present cytological data might be contributed to the taxonomic and evolutionary studies in the genus Cirsium.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.197-200
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• 1995

In this paper, we proposed the multi-step multi-layer artificial neural network(MMANN) to classify the chromosome, Which is used as a chromosome pattern classifier after learning. We extracted three chromosome morphological feature parameters such as centromeric index, relative length ratio, and relative area ratio by means of preprocessing method from ten chromosome images. The feature parameters of five chromosome images were used to learn neural network and the rest of them were used to classify the chromosome images. The experiment results show that the chromosome classification error is reduced much more, comparing with less feature parameters than that of the other researchers.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.107-116
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• 1997

Karyotype of apterous green peach aphid (Myzus persicae SuIBer) populations occurring in tobacco fields and their resistance to insecticides were investigated. All of the 16 clones collected from tobacco fields had same chromosome number of 2n=12. Among them, 5 clones had the normal karyotype, regardless of color morph, but chromosomal translnations between #1 and #3 autosomal chromosomes were noted in the other 11 clones. All of the aphid clones were highly resistant tolambda cyhalothrin, a pyrethroid pesticide, having over 20 times higher concentrations of LD90 than recommended concentration (10ppm) for the aphid. However, their resistance to acephate, demeton 5-methyl and pirimicarh varied depending on the clones, among which green-colored clones were generally more resistant to the pesticides than red-colored ones. No association was found between the insecticide resistance and the karyotype of the aphid in this experiment.

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.155-162
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• 2006

The chromosome numbers of five species in two families of Korea are investigated: Odontobutis platycephala, O. interrupta, and O. obscura in Odontobutidae, and Cottus koreanus and C. hangiongensis in Cottidae. In Odontobutis species, the three species showed the diploid chromosome number, 2n=44 (NF=44) telocentric chromosomes. In Cottus species, the mitotic chromosomes from 24 groups with two chromosomes each indicated that it is a diploid. The karyotype of C. koreanus and C. hangiongensis is 2n=48 and NF=52. These species is the first report on the chromosomes and the karyotype analysis except O. platycephala. Our findings provide cytotaxonomic evidence for the species distinctness of these five species whose descriptions were based primarily on external morphology.