• 약학회지
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• 제45권5호
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• pp.476-483
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• 2001

The clotting assay was replaced by the chromogenic substrate assay which is recommended by the European Pharmacopoeia (EP) and the Scientific and Standardization Committee of the International Society on Thrombosis and Haemostasis based on the reliability convenience and simplicity of the chromogenic assay, A correlation study was carried out with a one-stage factor Ⅷ : C clotting assay and the performance of the chromogenic assay was evaluated using two test kits that fulfilled the requirements of EP for factor Ⅷ concentrates test. Although chromogenic assay has partly differences in measurement principle and standardization, this assay has a high correlation with clotting assay in various types of factor Ⅷ concentrates and factor Ⅷ standard. We conclude that the chromogenic assay for factor Ⅷ : C concentrates correlates well with the clotting assay and shows good analytical performance.

• 한국균학회지
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• 제25권4호통권83호
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• pp.340-347
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• 1997

사과와 고추에서 분리된 Colletotrichum gloeosporioides의 색소형성형 및 유성세대형 계통균주들의 배양적 및 분생포자의 특성을 비교하였다. 유성세대 형성 계통들의 균사생장은 감자한천배지와 V-8배지에서 색소형성형계통에 비하여 빨랐다. 사과와 고추에서 분리된 색소형성형계통은 감자한천배지와 V-8배지에서 회백${\sim}$회록색의 균사가 환문을 이루면서 군데 군데에 황색${\sim}$연분홍색의 포자덩어리를 생성하였고 자낭포자세대를 형성하는 것은 없었다. 고추에서 분리된 계통은 사과와 감자한천배지에 한쪽 끝이 좁은 포자를 생성하였으나 V-8배지와 물한천잎배지의 것은 방추형이고 더 작았다. 사과에서 분리된 색소형성계통은 공시된 모든 배지에서 방추형의 분생포자를 생성하였다. 사과와 고추에서 분리된 유성세대 형성 계통들은 원통형의 포자를 형성하였는데 대부분은 양끝이 둥글었으며 감자한천배지와 V-8배지에서는 소흑점이 산재한 회백${\sim}$흑록색의 환문균층을 이루면서 자낭포자세대를 형성하였다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2002년도 International Meeting on Information Display
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• pp.268-269
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• 2002

Phtalocyanine derivative thin films were accumlated on ITO glasses by a Langmuir-Blodgett method. The layered structure and chromogenic properties of the films were investigated. We observed color changes which took place probably according to modulations of electronic distributions in the molecule. The novel chromogenic mechanism which can be applied to future FPD's was proposed.

• Bulletin of the Korean Chemical Society
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• 제30권1호
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• pp.197-200
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• 2009

A simple dye having phenol and indole moieties was synthesized and its chromogenic signaling behaviors for the determination of water content in organic solvents were investigated. The compound revealed a pronounced chromogenic behavior in response to the variation of water content in water miscible aprotic organic solvents of acetone, acetonitrile, THF, and dioxane. Significant red shifts and changes in absorption spectra allowed a ratiometric analysis of the signaling behavior. The chemosensing behaviors were particularly pronounced in water content in less than 10% that is suitable for the application of the compound as a probe for the determination of water content in binary aqueous organic solutions having lower water content.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.579-584
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• 2008

A commercial chromogenic agar medium (DFI) was supplemented with glucose (mDFI) to enhance the specificity of Enterobacter sakazakii (E. sakazakit) detection. Escherichia vulneris (E. vulneris), a putative false-positive strain on the DFI medium, produces ${\alpha}$-glucosidase. The enzyme ${\alpha}$-glucosidase hydrolyzes a substrate, 5-bromo-4-chloro-3-indolyl-${\alpha}$, D-glucopyranoside $(X{\alpha}Glc)$, producing green colonies. E. sakazakii strains produced green colonies on both DFI and mDFI agar, whereas E. vulneris produced green colonies on DFI agar but small white colonies on mDFI agar. E. sakazakii and E. vulneris were also readily differentiated by colony color when the mixed culture of the two strains was plated on mDFI agar and incubated for 24 h at $37^{\circ}C$. The results indicate that the selectivity of the commercial chromogenic agar medium could be improved by a simple supplementation with glucose.

• Mycobiology
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• 제34권2호
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• pp.108-110
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• 2006

To understand the ability of producing cellulolytic enzyme activity in the sapstaining fungi, four species of Ophiostoma and two species of Leptographium were investigated in the culture media containing each of cellulose substrates such as CM-cellulose, Avicel and D-cellobiose and each of chromogenic dyes such as Congo-Red, Phenol Red, Remazol Brilliant Blue and Tryphan Blue. When the fungi were grown for $5{\sim}7$ days at $25^{\circ}C$, the formation of clear zone by chromogenic reaction around the margin of the fungal colony was demonstrated in all the culture media Congo-Red containing CM-cellulose. There was difference in the formation of clear zone among the dyes. Only Ophiostoma setosum and Leptographium spp. showed cellulolytic activity to the three substrates. Overall, the results of this study show that ophiostomatoid sapstaining fungi can produce cellulolytic enzymes.

• 약학회지
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• 제43권5호
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• pp.606-613
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• 1999

To survey the possibility of replacing the pyrogen test with Limulus Amebocyte Lysate(LAL) test and to find out a standard methods suitable to our blood products made in Korea, 100 samples of 20% human serum albumin were tested by commercial LAL test kits and results of those were compared with rabbit pyrogen test. The LAL test is used both dinetic-chromogenically and kinetic-turbidimetrically. Both methods equally showed broad detection range (5.0~0.005 EU/ml), excellent sensitivity ($\geq$ 0.005 EU/ml) and predominant recovery rate within valid dilution range, but kinetic-turbidimetric method seemed to be more reproducible than kinetic-chromogenic method(kinetic-chromogenic method : S.D. = 15.88, kinetic-turbidimetric method : S.D. = 8.12). After heating the sample at 75$^{\circ}C$ for 15 min, the results showed a little elevated recovery rate with both methods. After performing the test on 100 albumin samples with both kits, the results were analysed using the USP standard (1.33 EU/ml). 7% of samples in kinetic-chromogenic methods and 1% of samples in kinetic-turbidimetric method exceeded the limit of endotoxin levels regulated for blood products in USA. Because this phenomenon was not observed in both methods at the same time and both methods have high sensitivity ($\geq$0.005 EU/ml), these results seemed to depend on nonspecific reaction. Considering its sensitivity and reproducibility, we could assure that LAL test is proper to detecting pyrogenic with good sensitivity.

• Journal of Dairy Science and Biotechnology
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• 제39권3호
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• pp.113-120
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• 2021

Although the number of incidences of illness caused by ingestion of the bacterial pathogen Enterobacter sakazakii (Cronobacter spp.) has dramatically declined, there remains a need for a robust isolation method to recover this microbe from powdered infant formula (PIF). The current method described in the FDA's Bacteriological Analytical Manual requires multiple steps, and 3-4+ days for complete analysis of PIF isolated E. sakazakii (Cronobacter spp.). We describe a bacteriological method including a one-step enrichment followed by plating on chromogenic agar for presumptive identification of E. sakazakii (Cronobacter spp.). Suspected colonies are confirmed by either biochemical analyses, or a Real-Time PCR-based assay. Using this method, E. sakazakii (Cronobacter spp.) in PIF can be isolated and identified within one day (24 hours).

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.717-720
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• 2003

A chromogenic biosensor employing microfluidics on a chip has been developed for the determination of high-density lipoprotein (HDL) cholesterol (HDL-C) in human serum. We have investigated a plain and effective method to immobilize enzymes within the microchip without chemically modifying micro-channel or technically micro-fabricating column reactor and fluid channel network. In assessing risk factors of coronary heart disease, a micro-chip system would minimize requirements of instrument and reagent handling.

• 한국수정란이식학회지
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• 제22권3호
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• pp.173-178
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• 2007

돼지 정자의 성 선별에는 일반적으로 유속 세포 분석기를 이용한다. 유속 세포 분석은 DNA량의 차이에 기초하여 정자를 분리하는 기술로써 X 정자와 Y 정자를 90% 정확도로 분리할 수 있다. 그러나 이러한 유속 세포분석 기술은 정자의 손상을 야기해 정자의 기능과 수정능에 영향을 미치므로, 본 연구에서는 특정한 핵산 서열을 탐지할 수 있는 Chromogenic in situ hybridization(CISH)을 그와 비교하여 평가하였다. 유속 세포 분석을 수행하기 위해 정자를 SYBR 14와 PI로 염색하였고, histogram, dotplot, density, contour를 측정하였다. Y 염색체 특이적인 primer를 이용한 PCR로 유속 세포 분석의 정확도를 검사하였다. HRP/DAB 시스템에 기초한 CISH 분석에는 X 또는 Y 염색체에 상보적으로 결합하는 probe가 사용되었다. CISH 분석은 기존의 방법들보다 빠르고 쉬우며 비용이 적게 든다는 장점이 있다. 또한, CISH는 보다 정화한 정자의 선별을 가능하게 하는 것으로 나타났다. 본 연구에 따르면 CISH가 기존의 선별 방법들을 평가하는 기술로서만이 아니라 특정한 성별을 가진 포유동물의 생산에도 사용될 수 있을 것이다.