• Title/Summary/Keyword: chorion

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Genetic analysis and characteristics in the crescent-egg mutant, cre, of Bombyx mori. (신돌연변이잠 cre(반월형란)의 유전자 연관분석과 유전형질)

  • 홍선미;노시갑
    • Journal of Sericultural and Entomological Science
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    • v.43 no.2
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    • pp.67-76
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    • 2001
  • The "crescent-egg" a new spontaneous mutant was detected in a white egg strain k37. Studies were carried out the linkage analysis, to investigate phenotypic characteristics and biochemical analysis of haemolymph and ovarian protein. The mutant, ore was independent from 20 linkage groups P(2), Ze(3),L(4), oc(5), sn8), Ia(9), w-1(10), K(11), ch(13), U(14), bl(15), cts(16), bts(17), mln(18), nb(19), oh(20),Lan(21), or(22), tub(23) and Xan(27). The fertilization, hatchability and larval growth were not different from the those of normal eggs. The content and composition of yolk protein were similared to normal eggs. Scanning electron microscopy revealed the areal specific structure in dorsal region of egg-shell of cre mutant. Analysis of chorion protein by isoelectrofocusing(IEF), was resolved no difference in the composite of the chorion protein. We conclude that the egg mutant ere is expressed only in the egg-shape formation and region specific determination.

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Analysis of Chorion Structures in Kidney Mutant, Bombyx mori (신장형란에 있어서 난각구조의 해석)

  • 노시갑
    • Journal of Sericultural and Entomological Science
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    • v.30 no.2
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    • pp.88-95
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    • 1988
  • The structure ki mutant chorion is described by the ultrastructural level with the scanning electron microscope. The micropyle on the anterior, posterior, leteral and dorsal side region surface patterns do not so much differ from ki mutant and normal strains. The surface structure of ventral side of ki mutant, which is the presumptive embryonic region in oocytes, were netted part corresponding to the boundary of follicle cells that is similar to lateral side structure of egg-shell. The eggshell thickness of ventral side on the ki mutant is mostly thicker. However, the structure of transverse section of normal and ki mutant egg-shell are composed of the outer, middle and inner layers. The ki not effects the formation of the lamellar layer in the egg-shell. It is observed that the characteristic structure in ki mutant egg chorion may be attributed to a areal specificity of epithelium in ventral side of egg-shell formation, and it affects the process of the accumulation of secreted chorionic material and the egg-shell architecture.

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Comparative preclinical assessment of the use of dehydrated human amnion/chorion membrane to repair perforated sinus membranes

  • Chang, Yun-Young;Kim, Su-Hwan;Goh, Mi-Seon;Yun, Jeong-Ho
    • Journal of Periodontal and Implant Science
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    • v.49 no.5
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    • pp.330-343
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    • 2019
  • Purpose: The aim of this study was to evaluate the use of dehydrated human amnion/chorion membrane (dHACM) to repair perforated sinus membranes in rabbits. Methods: Bilateral surgical windows (7.5-mm diameter) were prepared on the nasal bones of 14 rabbits. Standardized circular perforations (5-mm diameter) were made in the sinus membrane by manipulating implant twist drills. The perforated sinus membranes were repaired using dHACM or a resorbable collagen membrane (CM). The negative control (NC) group did not undergo perforated sinus membrane repair, while the positive control (PC) group underwent sinus augmentation without perforations. The same amount of deproteinized porcine bone mineral was grafted in all 4 groups. After 6 weeks, micro-computed tomography (micro-CT) and histomorphometric evaluations were conducted. Results: The micro-CT analysis revealed that the total augmented volume was not significantly different among the groups. In the dHACM group, newly formed bone filled the augmented area with remaining biomaterials; however, non-ciliated flat epithelium and inflammatory cells were observed on the healed sinus membrane. Histometric analysis showed that the percentage of newly formed bone area in the dHACM group did not differ significantly from that in the CM group. The dHACM group showed a significantly higher percentage of newly formed bone area than the NC group, but there was no significant difference between the dHACM and PC groups. Conclusions: dHACM could be a feasible solution for repairing sinus membrane perforations that occur during sinus floor augmentation.

A novel amnion-chorion allograft membrane combined with a coronally advanced flap: a minimally invasive surgical therapy to regenerate interdental papillary soft tissue recession - a six-month postoperative image analysis-based clinical trial

  • Pitale, Unnati;Pal, Pritish Chandra;Boyapati, Ramanarayana;Bali, Ashish;Varma, Manish;Khetarpal, Shaleen
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.47 no.6
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    • pp.438-444
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    • 2021
  • Objectives: Loss of the interdental papilla is multi-factorial and creates a multitude of problems. Autogenous connective tissue/biomaterial-based regeneration has been attempted for decades to reconstitute the black space created due to the loss of papilla. The aim of this present study was to regenerate papillary recession defects using an amnion-chorion membrane (ACM) allograft and to evaluate the clinical outcome up to six months postoperatively. Materials and Methods: Twenty patients with 25 Nordland and Tarnow's Class I/II interdental papillary recession defects were treated with ACM and coronal advancement of the gingivo-papillary unit via a semilunar incision on the labial aspect followed by a sulcular incision in the area of interest. A photographic image analysis was carried out using the GNU Image Manipulation software program from the baseline to three and six months postoperatively. The black triangle height (BTH) and the black triangle width (BTW) were calculated using the pixel size and were then converted into millimeters. The mean and standard deviation values were determined at baseline and then again at three and six months postoperatively. The probability values (P<0.05 and P≤0.01) were considered statistically significant and highly significant, respectively. An analysis of variance and post hoc Bonferroni test were carried out to compare the mean values. Results: Our evaluation of the BTH and BTW showed a statistically and highly significant difference from the baseline until both three and six months postoperatively (P=0.01). A post hoc Bonferroni test disclosed a statistically significant variance from the baseline until three and six months postoperatively (P<0.05) and a non-significant difference from three to six months after the procedure (P≥0.05). Conclusion: An ACM allograft in conjunction with a coronally advanced flap could be a suitable minimally invasive alternative for papillary regeneration.

The Influence of vibration during distribution of loose-formed eggs (Bombyx mori) to farmers (산란잠종배부시의 동요로 인한 영향)

  • 김원경
    • Journal of Sericultural and Entomological Science
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    • v.5
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    • pp.39-42
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    • 1965
  • The newly hatched silkworm can not come out with the head ahead, but on the contray, it comes out with the tail ahead when it comes out of the chorion, in case the loose-formed eggs are vibrated artificially, at the end of the incubation. So the newly hatched silkworm can not throw off the chorion alone which covers his head. This kind of eggs occurs about 30% when they are vibrated. In case they were raised, they could not grow well and some of them died. The result was quite bad. In conclusion, it would be better to distribute earlier the loose-formed eggs farmers and the loose-formed eggs must not be vibrated at the end of the incubation.

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Chinoperla rhododendrona, a New Species of Perlidae (Insecta: Plecoptera) from Vietnam

  • Cao, Thi Kim Thu;Bae, Yeon-Jae
    • Animal cells and systems
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    • v.11 no.2
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    • pp.125-128
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    • 2007
  • A new perlid stonefly, Chinoperla rhododendrona n. sp., is described from a tropical mountain stream in central Vietnam. The male adult of this species can be characterized by the strongly setous body and unique L-shaped genital structure. The female adult can be distinguished by the posteromedially sclerotized and widely notched subgenital plate. The egg is unique among its congeners as it has smooth chorion and long and narrow collar. Illustrations of diagnostic characters are provided.

Gametogenesis, Mating Behaviour and Spawning of Octopus ocellatus (Cephalopoda: Octopodidae) in Western Korea

  • Son, Pal Won;Kim, Byung-Gyun;Kim, Sung Han
    • The Korean Journal of Malacology
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    • v.31 no.2
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    • pp.113-121
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    • 2015
  • Gametogenesis, mating behaviour and spawning of Octopus ocellatus were investigated by histological study. This species is dioecious, and showed a protandry phenomenon. Ooogenesis (in females) and spermatogenesis (in males) can be classified into 3 stages, respectively. O. ocellatus copulates in one of two ways: a male may leap upon a female, mounting her mantle, or a male may sit near the female and extend the hectocotylized third right arm toward her. Spawning occurred between April and June in females, and between March and May in males of O. ocellatus. The spawning period was once a year and the peak took place between May and June. A number of flatened follicle cells, which were attached to an oocyte, were involved in vitellogenesis in the cytoplasm of the vitellogenic oocyte (maturing oocyte), and formation of chorion membrane (secondary egg membrane) of the ovarian eggs. Fecundity per female closely related to GSI was 294-660 eggs (average, 429 eggs). The diameters of the ovarian eggs surrounded by chorion membrane were approximately in the range of 10.10-2.50 mm. Each ovarian egg laid by a female was connected to an egg string. Each egg string was 1-5.5 cm (average 3.6 cm). The total number of eggs laid by a female of this species ranged 218-314, the egg sizes were independent to the size of female adult. this species has a life mode showing some special reproductive characteristics of an annual semelparity as shown in Octopodidae species because we have never seen a female spawning a second time.

Key for the Identification of the Ichthyoplankton in the Mid-East Coastal Waters of the Yellow Sea (黃海 中東部 沿岸域의 浮游性 卵 仔稚魚의 檢索表 作成 硏究)

  • 차성직;유재명
    • 한국해양학회지
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    • v.22 no.4
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    • pp.236-245
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    • 1987
  • Ichthyoplankton were sampled with Bongo net at 13 stations from Chonsu Bay to Keum Estuary from July, 1985 to June, 1986. 19 taxa of pelagic eggs and 45 taxa of larvae were collected. The key to identify the pelagic eggs is based on the number of oil gloubles, the shape of the egg, the sculpture of the chorion, and the size of the egg and the oil globules. The key to the families of fish larvae is based on the morphometric characters such as the body shape, the position of the anus, the pigmentation pattern, and others.

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