• Title/Summary/Keyword: chlorphyll

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Photosynthetic Characteristics and Cell Quota of Nitrogen and Phosphorus in Scenedesmus quadricauda under P Limitation (인제한에 따른 Scenedesmus quadricauda의 광합성 특성 및 질소, 이 함량 변화)

  • Ahn, Chi-Yong;Kim, Hee-Sik;Yoon, Byung-Dae;Oh, Hee-Mock
    • ALGAE
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    • v.17 no.2
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    • pp.83-87
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    • 2002
  • Photosynthetic parameters of Scendesmus quadricauda, such as the maximum photosynthetic rate ($P_{max}$), photosynthetic efficiency (α) and the initial saturation intensity of irradiance for photosynthesis ($I_K$) were obtained using photosynthesis-irradiance (P-I) curve in a phosphorus-limited chemostat. S. quadricauda exhibitied no photoinhibition until at 200 μmol·$m^{-2}$ . $P_{max}$ (r=0.963, P=0.002) and $I_K$(r=0.904, P=0.013) showed linear relationships with growth rate. Chlorophyll-α concentration and cell dry weight decreased at higher growth rates, ut chlorophyll-α content per cell dry weight increased. The increase in photosynthetic rates at higher growth rates was due to the increase of $P_{max}$ and $I_K$ which was caused mainly by the increase in the absolute amount of chlorophyll-α rather than the increased photosynthetic efficiency of individual chlorphyll-α. The α did not show a significant relationship with growth rate (r=0.714, P=0.111). The cell quota of carbon (r=0.554, P=0.254) was not correlated with growth rate, but cell quota of nitrogen (r=0.818, P=0.047) and phosphorus (r=0.855, P=0.030) exhibited linear correlations with growth rate.

THE RELATION BETWEEN QUALITY AND CONTENT OF ZINC AND MAGNESIUM IN DRIED LAVER, PORPHYRA TENERA KJELLMAN (건해태(김)의 아연 및 마그네숨 함양과 품질과의 관계)

  • LEE Jong-Ho;HAN Sung-Bin;LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.7 no.2
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    • pp.63-68
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    • 1974
  • The growth of sea weeds is greatly affected by the environmental conditions of ambient water. Especially nutrient salts in sea water function as a major factor to the growth of lavers, so that the content of inorganic substances in lavers could he a criterion for quality evaluation of lavers. In this experiment, the relation between the quality and the content of Zinc and Magnesium which are not only physiologically active and closely related with pigments such as chlorophll and phycobilins but also important in quantify is discussed if such a measurement to be an index for quality evaluation. Sixteen samples of layers were collected from three different culture farms, 7 from Jangrim-Busan, 6 from Wando-Jeon Nam, 3 from Hadong-Gyeong Nam, and classified into 3 quality grades to each farm and to whole samples by organoleptic test. Zinc and Magnesium were analyzed by atomic absorption spectrophotometry. For pigment analysis, chlorphyll was extracted with 85 percent acetone, filtered and the absorbance was measured at 660 nm and the residue was further extracted with phosphoric buffer solution to determine the optical density for phycobilins at 560 nm. The result showed that the total content of Zinc and Magnesium varied between the farms, but kept consistent with quality grades from the same farm. The Zinc content was correlated proportion- ally with the content of phycobilins, and Magnesium content in total ash could roughly represent the content of chlorophll except some examples in which the Magnesium content of chlorophyll-ext ractresiduewasexceptionallyhigher. In conclusion the contents of these metals provide an applicable index for quality judgment of lavers.

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Study on the Enzyme Activity in Leaf-Burning Disease of Panax ginseng C.A. Meyer (인삼엽요병에서 효소활성도의 변화)

  • 양덕조;김명원
    • Journal of Ginseng Research
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    • v.13 no.1
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    • pp.92-97
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    • 1989
  • This study investigated the effects of high light intensity (100 KLw) and high temperature (45 ℃, dark) on enzyme (glucose-6-phosphate dehydrogenase, acid phosphatase, catalase, peroxidase, and proteinase) activities and characteristics of Panax ginseng C.A. Meyer leaves. Enzyme activity and protein content decreased rapidly under treatment with high light intensity In P ginseng the thermal stabilities of catalase and peroxidase were high (above 70%), and the coagulation rates of soluble proteins were low (below 17%). Therefore, the decrease in enzyme activity and protein content was not caused by increase in leaf temperature due to the high light intensity, but by increase in proteolytic activities. The photochemical formation rate of superoxide radical (O-2) was higher in the P ginseng leaf extracts than in Solanum nigmm, and was accelerated by addition of crude saponin to the buffer extracts.

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Effects of ammonia gas on soybean plant (대두(大豆)에 대한 Ammonia 가스의 영향)

  • Kim, B.Y.;Han, K.H.;Kim, S.K.
    • Korean Journal of Soil Science and Fertilizer
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    • v.12 no.2
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    • pp.109-116
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    • 1979
  • This experiment was conducted to find out the effects on soybean plant exposed with various concentration and different fumigation periods of ammonia gas ($0.2mg/{\ell}$). The yield of soybean, nitrogen and, chlorophyll contents in leaves, and percentage of destroyed leaf area were investigated. The results were summarized as: 1. The soybean yield losses and percentage of destroyed leaf area were positively correlated with concentration of inflicting ammonia gas respectively. The yield losses was higher at noon exposure time than at night exposure time. 2. The soybean yield was negatively correlated with the percentage of destroyed leaf area. 3. The highest percentage of destroyed leaf area had at afternoon exposure time (14:00-15:00 o'clock) by $0.2mg/{\ell}$ ammonia gas fumigation, and the lowest one had at midnight exposure time (22:00-23:00 o'clock). 4. According to the increasing concentration of ammonia gas, the total and water soluble nitrogen contents in soybean leaves were increased, but the contents of chlorphyll b on decreasing rate. 5. The nitrogen contents in plant were higher in the afternoon exposure than in the morning, but the contents of chlorophyll were higher at night time exposure than at day time. 6. The highest decreasing of absorption spectra of chlorohpyll in chloroplast had at 10:00-11:00 o'clock on fumigation time and the lowest one had at 22:00-23:00 o'clock.

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The Agricultural Utilization of Daecheong Lake Sediments and Algae (대청호(大淸湖) 저니(底泥) 및 조류(藻類)의 농업적(農業的) 활용(活用) 연구(硏究))

  • Chang, Ki-Woon;Lim, Jae-Shin;Lee, In-Bog
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.2
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    • pp.111-116
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    • 1994
  • These studies were carried out to investigate the possibility on the agricultural utilization of lake sediment collected in the Daecheong Lake. Several parameters to estimate the degree of maturity of compost by windrow system were tested during a 50 day periods, and the results were as follows : During the composting of Daecheong Lake sediment, C/N ratio decreased gradually from 18.9 at the beginning to 13.1 at the final, while CEC increased from 33.5me/100g to 62.5me/100g. The temperature of lake sediment pile was the highest as $52^{\circ}C$ at 15th day and then decreased slowly until $30^{\circ}C{\sim}40^{\circ}C$ after 50 days. The results may be suggested that Daecheong Lake sediment compost reaches an acceptable level of stability after about 50 days. In order to know the effect of the Lake sediment and its compost on the growth of tomato(Lycopersicum esculentum M.), pot experiments were performed. The appiication of lake sediment(AS) itself, sediment organic fertilizer(SOF), and NPK mixed with sediment organic fertilizer(NSF) was more effective for tomato plant height, fresh and dry weight, and chlorphyll content than those of control treatment. Also, the lake sediment compost improved the physico-chemical properties of soil such as pH, total carbon, total nitrogen, available phosphorous and CEC.

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Eutrophication of Shellfish Farms in Deukryang and Gamagyang Bays (득량만과 가막양 패류양식장의 부영양화)

  • CHO Chang-Hwan;PARK Kyung-Yang;YANG Han-Serb;HONG Jae-Sang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.15 no.3
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    • pp.233-240
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    • 1982
  • Some environmental parameters on the shell-fish farms in Deukryang and Gamagyang Bays during summer in 1981 were determined to find an eutrophication level for the conservation of the farm. Chlorophyll-a content in the seawater in the Deukryang Bay in September was $1.0{\sim}5.0{\mu}g/l$ with an average of $2.5{\mu}g/l$ In the superficial bottom muds, contents of COD were 5-10 mg/g, ignition loss $5-9\%$, phaeophytin pigment $2{\sim}5{\mu}g/g$, and sulfide 0.1-0.3 mg/g dry mud in both bays. High contents of both organic matters and sulfide were found in the innermost area of the Deukryang Bay and in the north western part of the Gamagyang Bay. All quantities including chlorphyll-a in the water are little less than or similar to those of Hansan-Geoje Bay, one of the most productive shellfish farms in the southern coastal waters in Korea . Eutrophication on both water and bottom mud was under way like other shellfish farms but pollution indices on the bottom mud calculated from the data of CODs and phaeophytin pigments shelved 6-11, which is much lower than those of Jinhae Bay and of the Hansan-Geoje Bay. This means that the bottom muds are in an early stage of eutrophication, unlike the Jinhae and Hansan-Geoje Bays though the water, similar to the other bays, shows a middle stage of eutrophication.

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Effect of Salinity-stratified Waters on Upward Migration and Ratio of Extracted DNA/RNA in Cochlodinium polykrikoides Margalef Based on the Ratio of Absorbance at 260 and 280nm (염분 구배가 Cochlodinium polykrikoides Margalef의 수직이동 및 DNA/RNA 비율에 미치는 영향)

  • Cho Eun Seob;Lee Young Sik
    • Journal of Life Science
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    • v.15 no.3 s.70
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    • pp.468-473
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    • 2005
  • The coastal regions of Yeosu, the South Sea of Korea, has occurred annually the red tide which is caused by potentially ichthyotoxic dinoflagellate C. polykrikoides, with a wide avenue for exchange with oceanic waters and freshwater runoff from Sumjin river. We attempted to examine the variability in response to vertical migration and concentration of extracted DNA/RNA of C. polykrikoides exposed to salinity-stratified waters. The experimental aquarium of the 60 liter was employed to culture C. polykrikoides. One aquarium was supplied with only sea water, the other was consisted of sea water and freshwater. Experiment was conducted for 5 days. In experimental column (mixture of freshwater and sea water), salinity was maintained to 20 at upper and approximately 30 at bottom during the period of this study. The fluctuation with related to dissolved oxygen and pH was similar pattern to both columns. Chlorophyll a was significantly higher value at upper than bottom. During 24h, chlorphyll a on experimental column was extremely high on the top as soon as lighting, compared with control. With elapsed time, the gap between experimental and control columns was a little. In darkness, chlorophyll a was not significantly different between upper and bottom, most cells appeared to randomly distribute on column regardless of water layer. Fluctuation with related to concentration of extracted DNA and RNA based on ratio of absorbance of 260 and 280 nm in experimental column was higher at final day or diel migration than control. These results implied that a large volume of freshwater could be associated with influence of concentration of DNA and RNA, in particular, rapid upward movement caused massive fish kills as soon as sunset.

The variational characteristics of Water Quality and Chlorophyll a Concentration in Kogum-sudo, Southern Part of Korean Peninsula (거금수도 양식어장의 해양환경 특성 2. 수질과 엽록소 양의 변동특성)

  • 윤양호;박종식;고남표
    • Journal of Aquaculture
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    • v.13 no.1
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    • pp.87-99
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    • 2000
  • Field survey on the variational characteristics of water quality and chlorophyll a concentraion were carried out at the 25 stations for four seasons in Kogum-sudo(Straits) southern coast of Korean Peninsula from Feburuary to October in 1993. We made an analysis on biological factor as chlorophyll a concentraion as well as physicio-chemical factors such as water temperature salinity sigma-t transparency dissolved oxygen(DO) chemical oxygen demand(COD) nutrients (ammonia, nitrite, nitrate, phosphate, and silicate) N/P ratio and suspended solid(SS). The waters in the Kogum-sudo were not stratified due to the tidal mixing. And the high productivity in photic layer were supported by high nutrients concentration from bottom waters. The high concen-trations of suspended solid in straits had a bad influence upon marine biology of nature and cultivations. In Kogum-sudo had a sufficient nutrients for primary productivity during a year. Especially phosphate and inorganic nitrogen were high the other side silicate was very low. The source of nutrients supply depend on rather mineralization of organic matters and input of seawater from outside than input of freshwaters from lands. Phytoplankton biomass as measured by chlorphyll a concentratiion was very high all the year round and it was controlled by the combination o f several environmental factors especially of phosphate in summer and dissolved nitrogen in other seasons.

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Influence of Monochromatic Light on Conchocelis Growth and Maturation in Pyropia yezoensis (Rhodophyta) (단색광이 방사무늬김(Pyropia yezoensis) 사상체의 생장에 미치는 영향)

  • Jung, Sang-Mok;Kang, Seul-Gi;Son, Ji-Su;Jeon, Jae-Hyuk;Lee, Han-Joo;Jeon, Ji-Young;Park, Tae-Hee;Lee, Kwang-Soo;Shin, Hyun-Woung
    • Korean Journal of Environment and Ecology
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    • v.31 no.1
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    • pp.88-92
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    • 2017
  • This study investigated the growth and maturation of free conchocelis of Pyropia yezoensis under monochromatic light of blue light(480 nm), green light(550 nm), yellow light(600 nm) and red light(730 nm) which measured the colony diameter, content of chlorophyll a and conchosporangium formation. In the result, the most of colony diameter and chlorophyll a showed under the blue light, $2,472.6{\pm}27.0{\mu}m$ and $1.55{\pm}0.03mg\;g.dw^{-1}$. Also, the chlrophyll a content of conchocelis was under the blue light. Therefore, the blue light might be favorable for the growth and maturation of conchocelis of P. yezoensis. This study will lead development of indoor culture technologies.

Effects of Air Pollution on the Decline of Pinus thunbergii forest in Urban Industrial Area (도시공단의 대기오염이 해송의 쇠퇴에 미치는 영향)

  • Lee, Sung-Sik;Kim, Jeong-Woon;Lee, Chong-Kyu
    • Journal of agriculture & life science
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    • v.46 no.3
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    • pp.1-10
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    • 2012
  • This study was carried out to provide basal information on factors effected the decline of Pinus thunbergii by analysisof, the content of chlorophyll, and acid deposition through stepwise regression analysis. pH in throughfall at industrial area was 4.65 in the spring and 4.72 in the fall. On the contrary, pH at rural area was measured 5.32 in the spring and 5.34 in the fall. EC of rain fall at industrial area was $262.30{\mu}s$, However, there was $47.72{\mu}s$ at control area. Of anions, the concentration of ${NO_3}^-$ was $52.13mg/{\ell}$ at industrial area and $37.85mg/{\ell}$ at area. The concentration of ${SO_4}^{2-}$ was $57.89mg/{\ell}$ at industrial area and $36.21mg/{\ell}$ at area. Of the concentration of leaf chlorophyll in Pinus thunbergii, chlorophyll a was 0.2378 but control area was 0.4378. Also the content of chlorphyll b was 0.2097 and control area was 0.2345. The degree of decline of Pinus thunbergii forest was 2.97 at industrial area and 1.20 at area. We carried out a correlation analysis between the degree of decline and the concentration of ions, $SO_2$, and $NO_2$ in Pinus thunbergii forest. As a result, there was a negative correlation (r=-0.8672) between rain acids, and a positive correlation between $SO_2$ concentrations (r=0.8924) and between $NO_2$ concentrations (r=0.8428) in air. The correlations among acid depositions at level of 1% were pH (r=-0.8672), ${NO_3}^-$(r=0.6996), ${SO_4}^{2-}$(r=0.8497), $SO_2$ (r=0.8924), and $NO_2$ (r=0.8428).