• Journal of the Korean Chemical Society
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• v.26 no.4
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• pp.218-223
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• 1982

The absorbance and fluorescence yields of chl-a vs. concentration of n-prOH in diethyl ether, benzene and iso-octane were shown the characteristic point which chl-a structures are changed to monomer by the solvation of oligomer, and the spectral differences of fluorescence excitation between oligomer and monomer were identified by fluorimetry. All the maximum wavelength of absorbance, fluorescence excitation and fluorescence emission were shifted to longer wavelength. The ratios of soret/red band were depended on the band intensions and the polarities of solution in organic solvents mixed with n-prOH.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.E2
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• pp.89-98
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• 2006

Two tobacco cultivars (Nicotiana tabacum L.), Bel-B and Bel-W3, tolerant and sensitive to ozone, respectively, were grown in a greenhouse supplied with charcoal filtered air and exposed to 200 ppb ozone for 4 hr. Effects on chlorophyll fluorescence, net photosynthesis, and stomatal conductance are described. Quantum yield was calculated from chlorophyll fluorescence and the initial slope of the assimilation-light curve measured by the gas exchange method. Only the sensitive cultivar, Bel-W3, developed visual injury symptoms on up to 50% of the $5^{th}$ leaf. The maximum net photosynthetic rate of ozone-treated plants was reduced 40% compared to control plants immediately after ozone fumigation in the tolerant cultivar; however, photosynthesis recovered by 24 hr post fumigation and remained at the same level as control plants. On the other hand, ozone exposure reduced maximum net photosynthesis up to 50%, with no recovery, in the sensitive cultivar apparently causing permanent damage to the photosystem. Reductions in apparent quantum efficiency, calculated from the assimilation-light curve, differed between cultivars. Bel-B showed an immediate depression of 14% compared to controls, whereas, Bel-W3 showed a 27% decline. Electron transport rate (ETR), at saturating light intensity, decreased 58% and 80% immediately after ozone treatment in Bel-B and Bel-W3, respectively. Quantum yield decreased 28% and 36% in Bel-B and Bel-W3, respectively. It can be concluded that ozone caused a greater relative decrease in linear electron transport than maximum net photosynthesis, suggesting greater damage to PSII than the carbon reduction cycle.

• Journal of Photoscience
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• v.1 no.1
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• pp.47-52
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• 1994

Effects of HgCl$_2$-treatment on electron transport, chlorophyll a fluorescence and its quenching were studied using isolated barley (Hordeum vulgare L. cv. Albori) chloroplasts. Depending on the concentration of HgCI$_2$, photosynthetic oxygen-evolving activities of photosystem II (PS II) were greatly inhibited, whereas those of photosystem I (PS I) were slightly decreased. The inhibitory effects of HgCl$_2$ on the oxygen-evolving activity was partially restored by the addition of hydroxyamine, suggesting the primary inhibition site by HgCl$_2$2-treatment is close to the oxidizing site of PS tl associated with water-splitting complex. Addition of 50 $\mu$M HgCI$_2$ decreased both photochemical and nonphotochemical quenching of chlorophyll fluorescence. Especially, energy dependent quenching (qE) was completely disappeared by HgCl$_2$-treatment as observed by NH$_4$CI treatment. In the presence of HgCI$_2$, F'o level during illumination was also increased. These results suggest that pH gradient across thylakoid membrane can not be formed in the presence of 0 $\mu$M HgCl$_2$. In addition, antenna pigment composition might be altered by HgCl$_2$-treatment.

• ALGAE
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• v.30 no.2
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• pp.103-119
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• 2015

Photosynthetic rates of the large centric diatom Coscinodiscus granii were measured by means of multicolor variable chlorophyll fluorescence imaging, single cell $^{14}C$ assimilation, and optical $O_2$ sensor measurements during light acclimatization of cultures grown at five different irradiances: 50, 150, 235, 332, and $450{\mu}mol$ photons $m^{-2}\;s^{-1}$. Photo-acclimatization was evident from changes of cellular chlorophyll a content, growth rates, and light response curves. Each of the applied methods evaluates different parts and reactions in the photosynthetic apparatus, which makes a direct quantitative comparison of rates difficult, although a different degree of correlation were found between all three methods. However, when used in combination, they provide information about the internal relationship of photosynthetic pathways as well as the variation in photosynthetic capacity between individual cells within a single algal culture.

• Korean Journal of Medicinal Crop Science
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• v.16 no.6
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• pp.416-420
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• 2008

This study was conducted to investigate the influence of shading material on the chlorophyll fluorescence, photosynthesis, transpiration, stomatal conductance and its any correlations in Panax ginseng C.A.Meyer. Fo was higher in polyethylene shade net than in silver-coated shading plate, but this treatment caused a lower Fm in comparison with silver-coated shading plate. Also, Fv/Fm and PhiPS2 showed higher in silver-coated shading plate than in polyethylene shade net. The relationship between net photosynthetic rate and transpiration, stomatal conductance were increased as the PAR (Photosynthetic active radiation) was increased and reached maximum at the $200-400\;{\mu}mol/m^{2}/s$ of PAR in all of leaves, and the higher in silver-coated shading plate than in polyethylene shade net. A linear equation was obtained between net photosynthetic rate and transpiration, net photosynthetic rate and stomatal conductance. SPAD was higher in silver-coated shading plate than in polyethylene shade net.

• Journal of Sensor Science and Technology
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• v.33 no.1
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• pp.30-33
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• 2024

The occurrence of harmful algae on the coast of Korea has been a cause of damage to the aquaculture industry and deterioration of the coastal ecosystem environment. A method is required to predict their outbreak in real-time at the site. Therefore, this study attempted to develop a small hybrid optical sensor and real-time monitoring system based on LiDAR that can be used in the field and laboratory and can be applied to various platforms. FMS-L specifically suggested the amount of Chlorophyll a (Chl a) in the sample by measuring and analyzing the fluorescence emitted by the irradiating light. The accuracy of FMS-L was verified by measuring the concentrations of standard Chlorophyll a substances and Margalfidinium polykirkoids. In addition, the precision was verified by comparing the measurement results of FMS-L using commercial equipment Phyto-PAM-II. This equipment is compact and easy to move. Therefore, it can be easily applied to field surveys, allows short time measurements (10 s), and can be applied at a distance of 10 m from the measurement site.

• The Korean Journal of Ecology
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• v.19 no.2
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• pp.151-163
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• 1996

To investigate the early symptoms of light-chilling, alterations of chlorophyll fluorescence transients were monitored in cucumber (Cucumis sativus L. cv. Ilmichungjang) leaves. During 24 h chilling, decreases in (Fv)m/Fm, qE and qQ, and an increase in Fo were observed. The chilling effects were not recovered at room temperature, and a significant increase in Fo was observed during the recovery period. After 6 h chilling, ‘dip’(D) level of the transients became obscure, and the negative slope after ‘peak’(P) disappeared. The first derivative (dFv/dt) of the fast fluorescence rise curve was used to obtain more accurate information about the changes in the transients. The maximal rate of the fluorescence increase in the D-p rise curve (Fr) has been the most frequently used chilling stress indicator. However, a correct value of Fr could not be measured when the D level became obscure. This problem was overcome by introducing a new indicator, HFr (dFv/dt at Fv = 1/2 (Fv)m), and HFr gave very similar values to Fr. To monitor the changes in curvature around D level, another new parameter, ${\Delta}S$(D-Fr), was also introduced. These three parameters decreased very sensitively during light-chilling. In addition, increases in these parameters were observed during the first 2 h chilling, but this increase in Fr was also observed in pea leaf discs dark-chilled for 15 min, suggesting that this very early change is a common response to chilling in both pea and cucumber leaves. Quenching coefficients were also very sensitive to chilling, especially qE. Discussion on the usage of these parameters as chilling stress indicators is given in the text.

• Korean Journal of Environmental Agriculture
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• v.20 no.2
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• pp.93-98
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• 2001

A chilling-tolerant japonica-type and a chilling-susceptible indica-type of rice (Oryza sativa L.) plants were compared with respect to various physiological parameters during chilling and subsequent recovery. The japonica-type and the indica-type of rice cultivars used were Ilpumbyeo and Taebaekbyeo, respectively. The two rice cultivars exhibited little or no differences in the changes of leaf fresh weight and chlorophyll content during chilling at $5^{\circ}C$ for 3 days. During subsequent recovery at $25^{\circ}C$, however, Ilpumbyeo restored its growth more rapidly than Taebaekbyeo. Since the changes of relative water content, malondialdehyde production, an estimate of lipid peroxidation, and chlorophyll fluorescence were significantly different in the two rice cultivars during the chilling and subsequent recovery, they were found to be more sensitive physiological parameters than fresh weight and chlorophyll content. However, the differences in relation to water content, malondialdehyde production, and chlorophyll fluorescence between the two rice cultivars were smaller during chilling than those during subsequent recovery. These results suggest that relative water content, malondialdehyde production, and chlorophyll fluorescence could widely be used as important physiological parameters for screening chilling-tolerant plants.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.3
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• pp.196-207
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• 2019

This study was carried out to investigate the effects of C. japonica, D. morbifera, D. macropodum, I. anisatum, Q. glauca and R. indica To investigate the photosynthetic ability, chlorophyll content, chlorophyll fluorescence analysis, and physiological environmental. The photosynthetic rate, cancer respiration rate, stomatal conductance, and rate of evaporation tended to decrease as a result of drying stress in the no-water condition for 28 days. I. anisatum, Q. glauca and R. indica showed a low rate of less than 40% until 28 days of no-treatment. The total chlorophyll contents were decreased in the order of D. macropodum> D. morbifera> C. japonica> Q. glauca> M. thunbergii> R. indica> I. anisatum. Chlorophyll fluorescence analysis showed that there was no change in the qP, but after 28 days no $Fv/F_m$, $F_o$, $R_{fd}$, $NPQ_{_-LSS}$ can be a useful indicator for quantitative estimation within a short period of time with a marked reduction rate of PSII quantum yield ${\Phi}PSII$ in the rectified state by continuous light during the nominal adaptation period. In the case of I. anisatum, Q. glauca and R. indica If water management can be carried out at intervals, it may be possible to plant trees in trees and landscape trees.

• Journal of Photoscience
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• v.9 no.2
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• pp.385-387
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• 2002

Direct EPR evidence of the photo-generation of superoxide radicals ( $O_2$$^{-.}$) was obtained by using spin trapping techniques in spinach photosystem II (PSII) membranes. $O_2$$^{-.}$ was detected by following the formation of 5-diethoxyphosphoryl-5-methyl-1 -pyrroline-N-oxide (DEPMPO) superoxide adducts, DEPMPO-OOH. The significant increase of the EPR signal amplitude of DEPMPO-OOH in N$H_2O$H-, CaC $l_2$- and NaCl-treated PSII membranes showed that the oxygen-evolving system has a close relation to the $O_2$$^{-.}$ production. PSII membranes with inactivated donor side could not prevent the $O_2$$^{-.}$ production efficiently. Treatments on PSII donor side also influence the maximum level and the kinetics of Chlorophyll (Chi) a fluorescence. Results suggested that manganese cluster and extrinsic proteins might affect Chi a fluorescence in ways different from that happens at the acceptor side of PSII.SII.SII.