• Journal of Food Hygiene and Safety
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• v.2 no.2
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• pp.83-88
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• 1987

Effects of the extraction-methods and solvents on some minor components in crude rapeseed-oil and the autooxidation of the oils were examined in this study. The contents of unsaponifiable matters and sterols were not significantly variied depending on the extraction-methods and solvents. Chlorophyll-contents were elevated with the increasing molecular weights of extractionsolvents. Any significant effect of the extraction-temperature on the yield of chlorophyll was not observed. Autoxidation-tendency of the oils was found to be dependent on the boiling points of extraction-solvents.

• Proceedings of the KSRS Conference
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• v.2
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• pp.918-921
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• 2006

The short-term variation of sea surface temperature before and after typhoons and increase of chlorophyll a concentration that accompany with the typhoons during summer in the East/Japan Sea were explored by satellite. Four typhoons (NAMTHEUN, MEGI, CHABA and SONGDA) and a typhoon (NABI) passed over the East/Japan Sea in 2004 and 2005, respectively. Decreasing of SST was observed in the every five typhoons, however the magnitude of SST decreasing were various from 1 to $5^{\circ}C$. Chlorophyll a increases were found after the typhoons (0.1-3 ${\mu}g$ $l^{-1})$ except NAMTHEUN, and the area was approximately included in SST decreasing area by the typhoons. It suggests that chlorophyll a increase was caused by nutrient input from subsurface layer by strong mixing. On the other hand, rarely chlorophyll a increase was observed in northern area of polar frontal zone, which is located in $38-41^{\circ}N$, than northern area, and chlorophyll a increase in coastal area was higher (more than 3 times) than offshore area. It might suggest that chlorophyll a increase in the East/Japan Sea is also related with the depth or nitracline depth that affects the amount of nutrients supply to the upper layer by typhoon mixing.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.3
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• pp.441-452
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• 1996

The effects of chlorophyll, tocopherols($\alpha$-tocopherol, ${\gamma}$-tocopherol and $\delta$-tocopherol), carotenoids ($\beta$-carotene and lutein), light sources, light intensities and strage temperatures on the photooxidative stability of soymilk were studied by measuring TBA value and depleted headspace oxygen(DHO) of soymilk. The samples were stored in the light storage box for 6 days and evaluated for the photooxidative stabilities. As the concentrations of chlorophyll increased, TBA value and DHO of the sample increased significantly(p<0.05), indicating chlorophyll acting as a photosensitizer. However, as the concentrations of tocopherols ($\alpha$-tocopherol, ${\gamma}$-tocopherol and $\delta$-tocopherol) and carotenoids ($\beta$-carotene and lutein) increased, TBA values and DHO of the samples decreased significantly(p<0.05). The light screening effects of carotenoids on DHO in the samples were not significantly different from the control at p>0.05. Therefore, there was no light screening effects of carotenoids on the oxidative stability of soymilk. The results indicate that tocopherols and carotenoids reduce the photooxidative stability of soymilk. $\delta$-Tocopherol was the most effective in photosensitized oxidation followed by ${\gamma}$-and $\alpha$-tocopherols in the order of increasing stability. $\beta$-Carotene was significantly(p<0.05) more effective than lutein in minimizing the chlorophyll-sensitized photooxidation of soymilk. Visible light was more effective than UV light in decreasing the photooxidative stability of soymilk. Therefore, photooxidation of soymilk containing chlorophyll is mainly due to photosensitized oxidation rather than photolysis reaction. As the intensities of fluorescence light increased, TBA values and DHO of the samples increased significantly at P<0.05. However, as the storage temperatures increased, TBA values and DHO of soymilk did not change significantly at p>0.05.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.6
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• pp.637-643
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• 2019

Three separate feeding trials were performed to evaluate the dietary inclusion of pigments on growth and pigmentation of Semisulcospira coreana. In the first trial (Exp-1), snails (80 mg/snail) were fed diets containing green laver Enteromorpha intestinalis, seaweed fulvescens Capsosiphon fulvescens, chlorella Chlorella vulgaris, green tea, mugwort, kale, broccoli, sea tangle, dried laver Pyropia yezoensis, a synthetic edible dye, and a diet containing no pigment (control) for 12 weeks. Results showed that dietary treatments had no significant effects on growth performance. However, total chlorophyll and chlorophyll a contents of the snail's edible portion were markedly affected and the highest values were found in snails fed green laver. In the second (Exp-2) and third (Exp-3) experiments, the effect of green laver and chlorella were examined in diets for relatively smaller (35 mg/snail) and bigger (139 mg/snail) snails than those examined in the Exp-1, respectively. Feeding the bigger snails with chlorella significantly enhanced their growth rates compared to control group. Total chlorophyll and chlorophyll a contents of the edible portions of snails were significantly increased by dietary chlorella supplementation. These findings suggest that dietary green laver or chlorella could improve the pigmentation of S. coreana without any adverse effects on growth.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.98-104
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• 1989

In order to determine the specific active oxygen species directly related to chlorophyll bleaching in the leaf-burning disease, we investigated the effects of singlet oxygen (1O2), superoxide radical (O2-), and hydrogen Peroxide (H2O2) on isolated chloroplast suspension and leaf discs from Panax ginseng C.A. Meyer. When the singlet oxygen was added to the chloroplast suspension, the chlorophyll and carotenoid contents were decreased by more than 809), similar to treatment with high light intensity (100 KLux). We assumed that the conversion of dioxygen (O2) produced either in photolysis or in breakdown of hydrogen peroxide to singlet oxygen resulted from photorespiration. On the basis of these experiments , :he inhibitory effects of active oxygen scavengers propylgallic acid (PGA), 2,5-ditetrabutyl hydroquinon (DBH), sodium pyrosulfate (SPS), and ascorbic acid (ABS) were examined. In chloroplast suspension all four scavengers inhibited chlorophyll bleaching by more than 75fl , and in the leaf discs the inhibition rates of SPS, PGA and ABS were 46%, 51%, and 96% respectively.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.6
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• pp.950-957
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• 2009

Mustard Leaf Kimchi (MLK) is a traditional fermented Korean vegetable food. This study was conducted to investigate the effects of part vacuum treatment on MLK packed in a glass bottle during fermentation. There have been a few previous studies that examined the chemical and microbial changes during MLK fermentation. However, the major object of this study was to investigate the antioxidative activities of vacuum treated MLK. In this study, the antioxidative activity of vacuum treated mustard leaf Kimchi (VM) and control mustard leaf Kimchi (CM) were examined. VM and CM were fermented at $5^{\circ}C$ for 8 weeks. A model system was designed to evaluate the antioxidative activity of crude chlorophylls and carotenoids (CCC) extracts from Mustard leaf Kimchi. The oxidative reaction of the linoleic acid mixture system at $30^{\circ}C$ in the dark was quantified determining the peroxide value and conjugated dienoic acid content. The effect of the CCC extracts on lipid peroxidation in a rat liver homogenate was examined. Formation of lipid peroxides was estimated by the TBA value, and the CCC extracts were found to inhibit the TBA value. Chlorophyll a and b, and carotenoids, Which are the major components in the CCC extracts of Kimchi were isolated on a DEAE-sepharose CL-6B and Sepharose CL-6B column and TLC. The effects of chlorophyll a and b, caroteins on linoleic acid autoxidation were measured by determining the peroxide value. In addition, their effects on free radical scavenging were investigated by DPPH. In this assay, chlorophyll a showed the greatest antioxidative activity followed by chlorophyll b, and carotenoids. MLK contains a sufficient content of chlorophyll a and b, and carotenoid which have strong antioxidative activities.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.4
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• pp.280-286
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• 2007

In order to find out the effects of the anticyclonic eddy on the distribution of nutrients and chlorophyll concentrations in the Ulleung Basin during spring and summer, we measured temperature, salinity, nutrients, and chlorophyll from the surface to 200 m water depth at five stations in July 2005 and April 2006. In spring, surface mixed layer was very deep inside the eddy, about 200 m, but it was relatively shallow outside the eddy, about $20{\sim}60$ m. Inside of the eddy, nutrients in the surface waters were sufficient by supply from the deep layer, whereas outside of the eddy, they were fairly depleted due to the stratification in the surface layer. In spring, chlorophyll concentrations were relatively low inside of the eddy due to the deeper surface mixed layer compared with the euphotic depth, and the depth-integrated chlorophyll concentrations outside of the eddy were twice as much as those inside of the eddy. In summer, nutrients in the surface waters were completely depleted at all stations due to the well stratification in the surface layer. The typical distribution pattern of subsurface chlorophyll maximum was observed at all stations, and the depth-integrated chlorophyll concentrations inside of the eddy were almost twice as much as those outside of the eddy. The anticyclonic eddy appearing in the Ulleung Basin every year significantly affects the phytoplankton biomass, with the opposing effects in spring and summer; in spring, the anticyclonic eddy suppresses phytoplankton growth, but in summer, it enhances the phytoplankton biomass.

• Journal of Environmental Science International
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• v.24 no.12
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• pp.1591-1600
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• 2015

The response of the freshwater microalga, Chlorella vulgaris, to heavy metal stress was examined based on chlorophyll fluorescence analysis to assess the toxic effects of heavy metals in freshwater ecosystems. When toxic effects were analyzed using regular chlorophyll fluorescence analysis, photosystem II activity($F_v/F_m$) decreased significantly when exposed to $Cu^{2+}$ and $Hg^{2+}$ for 12 h, and decreased in the order of $Hg^{2+}>Cu^{2+}>Cd^{2+}>Ni^{2+}$ when exposed for 24h. The effective photochemical quantum yield(${\phi}{\prime}_{PSII}$), chlorophyll fluorescence decrease ratio($R_{Fd}$), minimal fluorescence yield($F_o$), and non-photochemical quenching(NPQ), but not photochemical quenching(qP), responded sensitively to $Hg^{2+}$, $Cu^{2+}$, and $Cd^{2+}$. These results suggest that $F_v/F_m$, as well as ${\phi}{\prime}_{PSII}$, $R_{Fd}$, $F_o$, and NPQ could be used to assess the effects of heavy metal ions in freshwater ecosystems. However, because many types of heavy metal ions and toxic compounds co-occur under natural conditions, it is difficult to assess heavy metal toxicity in freshwater ecosystems. When Chlorella was exposed to heavy metal ions for 12 or 24h, $F_v/F_m$ and maximal fluorescence yield($F_m$) changed in response to $Hg^{2+}$ and $Cu^{2+}$ based on image analysis. However, assessing quantitatively the toxic effects of several heavy metal ions is challenging.

• Korean Journal of Food Science and Technology
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• v.44 no.6
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• pp.680-685
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• 2012

The effects of chlorophyll (0.33 mg/kg) and ${\beta}$-carotene (3.3, 9.9, 19.8 mg/kg) addition to a mixture of roasted perilla seeds, rice, and water (30:45:225, w/w/w) on the lipid oxidation and tocopherol contents were studied during heating at $100^{\circ}C$ for 120 min to simulate cooking of perilla and rice porridge. Lipid oxidation was evaluated with peroxide values (POV) and conjugated dienoic acid (CDA) values, and chlorophyll, ${\beta}$-carotene, and tocopherols were determined by HPLC, POV, and CDA values were increased during heating, indicating the occurrence of lipid oxidation in the perilla and rice porridge. ${\beta}$-Carotene decreased the POV and CDA values of the samples in a concentration-dependent manner, while the addition of chlorophyll did not affect them. Chlorophyll and ${\beta}$-carotene which were added, and tocopherols naturally present in samples were degraded, following the first order kinetics during heating, and ${\beta}$-carotene protected tocopherols from degradation.

• Journal of Environmental Science International
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• v.5 no.4
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• pp.545-553
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• 1996

The effects of 0.2 ppm ozone on the developing chloroplast of barley (Hordeum vulgare L.) seedling during greening were examined by chlorophyll contents, photosynthetic electron tiansport activity, Fo, Fv and fluorescence quenching coefficient. Chlorophyll contents of seedling treated with ozone were not changed in comparison with the control during the 96 h greening experiment, but PS II activity of the chloroplasts of seedlings treated with ozone was decreases by- l5%. Fo was slightly decreased but Fv was decreases by 5% in comparison with the control, In fluorescence quenching analysis, qP and qE were decreased by 11% and 9%, respectively, in comparison with the control. These results suggest that oxidation site of PS II is the site affected mostly and PQ pool is also affected slightly by 0.2 ppm ozone.