• 대한예방한의학회지
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• 제9권2호
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• pp.125-134
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• 2005

It is well known that Crataegus Fructus strengthens the Stomach and Spleen, helps digestion, tonify stomachache, and decreases bleeding. A major component isolated from this herb consist of carotene, riboflavin, quercetin, chlorogenic acid, amygdalin, ursolic acid etc. In this study, we aimed to analyze the changes of liquid chromatogram pattern, one of major standardization method, to determine the quality of Crataegus Fructus.

• Natural Product Sciences
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• 제16권3호
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• pp.198-201
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• 2010

Acanthopanax divaricatus var. albeofructus is one of the indigenous medicinal plant and the fruits of Acanthopanax spp. used as a remedial for "wipe out evil wind". Two anthocyanin were isolated from the fruits of Acanthopanax divaricatus var. albeofructus. Their structures were elucidated as cyanidin 3-lathyroside (1) and cyanidin 3-galactoside (2) by chemicophysical and spectroscopic analysis. And also, four chemical, syringin, chlorogenic acid, caffeic acid and acanthoside D were identified. Both anthocyanide were isolated for the first time from Acanthopanax species. cyanidin 3-lathyroside is one of the rare anthocyanin in natural resources.

• 한국식품저장유통학회지
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• 제12권5호
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• pp.489-495
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• 2005

우엉 뿌리(Arctium lappa L.)에서 polyphenol oxidase가 DEAE-Cellulose 음이온 수지, 황산암모늄 침전법, Phenyl-sepharose CL-4B 친화크로마토그래피 그리고 Sephadex G-100 겔여과크로마토그래피 등의 과정으로 정제되었며 정제효소의 효소학적 특성을 검토하였다. 정제된 효소의 분자량은 약 30 kDa의 단일폴리펩티드 사슬로 구성되어 있었다. 효소반응의 최적 pH와 온도는 각각 7.0과 35$\circC$이었으며, pH 2와 6 사이의 산성조건과, pH 8과 10 사이의 알카리 조건에서는 활성이 감소되거나 상실되었다. 온도에 대한 영향을 살펴본 결과, 40$\circC$까지 비교적 안정한 활성을 나타내고 있었으나, 50$\circC$에서 30분간 정치 시 효소활성을 50%이상 감소하는 것으로 나타내었다. 이 효소는 catechol과 L-DOPA에 대하여 높은 효소활성을 나타내었으며, chlorogenic acid과 catechol에 대한 Km 값은 각각 16.18 mM과 23.11 mM이었다. Ascorbic acid와 L-cysteine은 우엉 뿌리 polyphenol oxidase의 효소활성을 감소시켰으며, 금속이온의 경우 $Cu^{2+}$가 효소의 활성을 감소시키는 인자로 나타났다.

• 한국식품영양학회지
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• 제32권4호
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• pp.312-320
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• 2019

This study examines the changes in chlorogenic acid (CGA), an antioxidant, and one of its decomposition substances, caffeic acid, at various roasting stages and extraction conditions. Based on the CGA content for each roasting stage, at $3^{\circ}C$ after the beginning of the $1^{st}$ crack, the CGA decreased for washed beans and natural beans by more than 50% compared to that of green coffees. The CGA continued to decrease sharply by more than 75% at the end of the $1^{st}$ crack for washed beans and at $5^{\circ}C$ after the end of the $1^{st}$ crack for natural beans. At the peak of the $2^{nd}$ crack, it had decreased by more than 90% for both beans. The Caffeic acid content gradually increased for both washed and natural beans, then rapidly increased from the beginning of the $2^{nd}$ crack to the peak of the $2^{nd}$ crack. However, its contents were very small in quantity. Additionally, the content of CGAs for differing extraction conditions were in the order of 3-CGA, Crypto-CGA, and Neo-CGA. Crypto-CGA content was about half that of 3-CGA and Neo-CGA content was approximately 100 ppm less than that of Crypto-CGA. This study was conducted in order to help make coffee that has the most antioxidant effect.

• 분석과학
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• 제14권5호
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• pp.377-383
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• 2001

사과에 분포하는 페놀계 물질의 총 함량을 분석하는데 가장 적절한 방법을 확립하기 위하여 우리나라에서 재배되는 4품종을 각각 5개 지역에서 수집한 20개 종류의 사과를 시료로 가장 효율적인 분석조건을 조사하였다. 천연재료 줌의 총 페놀 정량방법으로 이용되고 있는 분광광도법인 Foline-Dennis법, Prussian-blue법 그리고 vanillin-HCI법을 각각 시료에 적용하여 총 페놀 함량을 측정하여 고찰하였다. 그 결과 chlorogenic acid 상당량으로 산출한 Folin-Dennis법이 사과의 총 페놀 정량방법으로 가장 적합하였다.

• Journal of the Korean Wood Science and Technology
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• 제43권5호
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• pp.591-599
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• 2015

Cercidiphyllum japonicum leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. A portion of EtOAc fraction (10 g) was chromatographed on a Sephadex LH-20 column, by the successively elution with various aqueous $MeOH-H_2O$ (1:9, fraction $1-2{\rightarrow}3:7$, fraction $3-5{\rightarrow}1:1$, fraction $6-9{\rightarrow}7:3$, fraction $10-13{\rightarrow}9:1$, fraction 14-16). Compound 2 was isolated from fraction 6 and compound 1 was separated from fraction 11 and 12. Compound 3 and 4 were purified from fraction 13. The isolated compounds were elucidated as quercetin-3-O-${\alpha}$-L-rhamnopyranoside (1), chlorogenic acid (2), quercetin-3-O-${\alpha}$-L-arabinofuranoside (3) and quercetin-3-O-${\beta}$-D-xylopyranoside (4) by the spectral and literature data, and by comparison with the authentic samples. These compounds were reported, for the first time, from the extracts of C. japonicum leaves. Also chlorogenic acid (2) has never been reported before in domestic tree species and can be used as an index compound for C. japonicum.

• 한국자원식물학회지
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• 제27권6호
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• pp.629-634
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• 2014

Rice type tartary buckwheat is used as a substitute for rice in many Asian countries due to its easy dehulling character. The objective of the present study was to determine the chlorophyll, total polyphenol (TP), total flavonoid (TF), antioxidant activity and to quantify the bioactive compounds rutin, quercetin and chlorogenic acid in the seedlings of rice-type tartary buckwheat (RTTB). Young seedlings exhibited higher antioxidant (DPPH radical inhibition) activity in dose dependent manner. TP and TF content were highest ($3017.46{\pm}201.84{\mu}g$ TAE/100 mg dw and $1916.0{\pm}102.95{\mu}g$ QE/100 mg dw respectively) in 3 days after germination (DAG) seedlings compare to 6 and 9 DAG. The contents of rutin and quercetin increased with growing stage of seedlings. However, the chlorogenic acid decreased with increasing growth. Overall, RTTB seedlings can be regarded as a strong source of phenolics and have high possibility for food and nutraceutical application due to their efficient antioxidant properties, higher chlorophyll and phytochemical content.

• 약학회지
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• 제32권5호
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• pp.328-333
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• 1988

Based on the following tests with fractions extracted from organic solvents such as benzene, chloroform, ethyl acetate and methanol, this study is to analyse the antimutagenicity of Lonicerae Flos. When carrying out Ames test with Salmonella typhimurium strain, it seemed that there was stronger antimutagenicity in TA 100 treated by MNNG than did one in TA 98 by NPD, and that there was stronger antimutagenicity through base pair exchange than one through frame shift. In the umu test, each fraction tended to inhibit the activity of ${\beta}-galactosidase$ induced by AF-2. As shown in these above tests, the ethyl acetate fraction was the strongest among four fractions. On the other hand, its component consisted of luteolin, apigenin, chlorogenic acid and five unknown ingredients. Of these unknown ingredients, E, F strongly tended to inhibit the activity of ${\beta}-galacosidase$. In addition,there was also the decrease in its activity of apigenin, luteolin and chlorogenic acid.

• 한국자기공명학회논문지
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• 제21권2호
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• pp.44-49
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• 2017

Coffee is one of the top selling products in the world. There are various coffee bean species around the world. Among them, Coffea Arabica is the most popular species. However, there are few studies on the metabolites of coffee beans so far. This study demonstrates effects of the planted regions on the metabolite concentrations of coffee beans. The metabolites of coffee beans can be affected by growing area even although same species are grown. Accordingly, we studied coffee bean metabolites extracted from the same species in different regions (The brand names, Kona from Hawaii, Mocha Matari from Yemen, and Blue Mountain from Jamaica) by using mixed solvent of methanol: water: chloroform. A comparative analysis by NMR spectroscopy was performed and the statistical techniques were used to figure out the differences. As a result, we found that chlorogenic acid, caffeine, citrate, and sucrose mainly contributed to the separation of the three groups. When compared with Kona and Blue Mountain, concentrations of chlorogenic acid, caffeine, and sucrose in Mocha Matari were observed to be relatively down-regulated. In addition, compared with the two other groups, concentration of citrate in Kona was observed to be up-regulated.

• 생약학회지
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• 제50권2호
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• pp.124-132
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• 2019

This study compared the contents of phenolic components depending on the extracting conditions of Acanthopanax koreanum stem to provide basic information for developing Acanthopanax koreanum-based functional foods. Our findings show that the content of total phenolic component peaked at 16 hours of extraction (WAK-16, 7.22%) and when water extracted at $100^{\circ}C$. However, 11-hours water extraction (WAK-11) showed highest eleutheroside B concentration of 1.32%, a main component of A. koreanum, and the level of chlorogenic acid concentration was the highest when 1-hour water extraction (WAK-1) was conducted, being 2.12%. Moreover, highest concentration of eleutheroside E was observed in 16-hours water extraction (WAK-16) as 1.49%. With 60-hours water extraction (WAK-60), the content of syringaresinol, an active phenolic aglycon substance, concentration was the highest with the value of 0.10%. Isofraxidin showed the highest concentration of water extract(0.09%) for 20 hours (WAK-20) and 0.42% for sinapyl alcohol[16 hours (WAK-16)].