• Title/Summary/Keyword: chloride media

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A Study on Corrosion CoCrMo Magnetic Thin Films (CoCrMo 자성박막의 부식에 관한 연구)

  • 남인탁;홍양기
    • Journal of the Korean Magnetics Society
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    • v.3 no.3
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    • pp.221-228
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    • 1993
  • The general requirements of recording media include recording performance, environmental stability, runnability on the drive or deck, and manufacturability. CoCrMo thin films were prepared using RF sputtering system for a study on chemical stability. Surface degradation of the CoCrMo thin film was studied by SEM, XPS and AES. Surface degradation was found to be dependent of sputtering condition and Mo content. Addition of Mo to CoCr thin film improved dramatically its surface degradation resistance in dilute sulfuric acid, as indicated by active-passive transition appeared in electrochemical polarization curve. Futhermore, the passive current density was decreased with increasing Mo content. The reduction in a number density of corrosion sites by Mo addition vms observed, after accelerated corrosion test. AES survey indicated that corrosion occured on the site with Cr depletion and highly concentrated chloride ions.

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Identification and Characterization of Hemolytic Bacillus cereus Isolated from Commercial Ssam-jang (시판 쌈장에서 분리한 용혈성 Bacillus cereus의 동정 및 특성 조사)

  • Kim, Dong-Min;Park, Sang-Kook;Oh, Kye-Heon
    • KSBB Journal
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    • v.32 no.3
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    • pp.179-186
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    • 2017
  • This study was undertaken to identify and characterize hemolytic Bacillus cereus isolated from commercial ssam-jang. The physiological and biochemical properties of isolate were first examined. Using the BIOLOG system, the isolate was identified and assigned to B. cereus MH-2. Phylogenetic tree of MH-2 was plotted based on 16S rRNA sequence comparisons. Hemolytic activity was observed around wells of sheep blood agar plates seeded with MH-2 cultures; the zone of hemolysis gradually increased with increasing incubation time of the cultures. Zymographic analysis estimated the molecular weight of the presumed hemolysis-causing molecule to be about 30 kDa. Survival rates of MH-2 cells decreased with increasing NaCl concentrations in the media. The stress shock proteins (e.g., DnaK and GroEL) induced by NaCl were reduced in proportion to the NaCl concentration and exposure period to B. cereus MH-2. Analysis of SDS-PAGE and Western blot revealed that the stress shock proteins, 70-kDa DnaK and 60-kDa GroEL were decreased proportionate to the NaCl concentrations as well as exposure period in exponentially growing cultures. Scanning electron microscopy demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with NaCl.

Collection Characteristics of a MOUDI Cascade Impactor for Coarse Particles (다단 임팩터(MOUDI)의 조대 입자 채취 특성)

  • 배귀남;지준호;문길주
    • Journal of Korean Society for Atmospheric Environment
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    • v.15 no.6
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    • pp.799-804
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    • 1999
  • Particle collection characteristics of the MOUDI cascade impactor has been studied for coarse particles in the range of 2 to 20$mu extrm{m}$ in aerodynamic diameter. A vibrating orifice aerosol generator was empolyed to generate monodisperse test aerosols. The oleic acid and sodium chloride(NaCl) particles were used as test aerosols. Aluminum foil and Teflon filter were selected as impaction media. The sampling flow rate was changed from 25 to 35L/min. Particle collection efficiency for single stage was examined for liquid particles. The stage response was obtained experimentally for the cascade impactor composed of three stages and a backup filter. The results showed that most of particle collection efficiencies measured in this work are similar to the efficiency curves obtained by Marple et al.(1991). For particles less than cut-off size of the stage, the collection efficiencies of solid particles are similar to those of loquid particles. However, the collection efficiency of solid particles decreases with mereasing particle diameter for the particles greater than the actual cut-off size of the impactor. The particle collection efficiency increases with increasing sampling flow rate at the same particel size. However, the collection efficiency curves seem not to be greatly shifted with the flow rate. The stage responses obtained by direct measurements in this work are in good agreement with those derived from the collection efficiency curves for single stage.

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Precious Metals Extraction Processing in Chloride Media by Using Ionic Liquids as Novel Extractant Systems

  • Kumar, Jyothi Rajesh;Choi, In-Hyeok;Lee, Jin-Young
    • Korean Chemical Engineering Research
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    • v.55 no.4
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    • pp.503-509
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    • 2017
  • The present experimental study proposed two ionic liquids (ILs) namely [Aliquat 336] [$HSO_4$] (prepared and characterized at our laboratory) and Cyphos 101 IL (supplied by Cytec Company) dissolved in two different diluents such as DCM (di-chloro-methane) and toluene applied for PMs extraction. The first IL [Aliquat 336] [$HSO_4$] prepared and confirmed the formation of final product by using FT-IR and TGA studies. The primary experiment in solvent extraction processing is kinetic effect; 0 to 30 time varied for PMs by using two ILs and confirmed the optimized extraction equilibrium time. This study was conducted for PMs (Pt, Rh and Cu) extraction and separation from each other by using proposed ILs. This is the primary study of the utilizing green solvents such as ILs as an extractant system for Pt, Rh and Cu extraction and possible separation.

Comparative Study of Corrosion Inhibition in Acidic and Neutral Chloride Media by Some Amino Acids (염산과 NaCl 수용액에서 알루미늄의 부식에 미치는 아미노산의 부식억제효과)

  • Yoon, Jonghwa;Kim, Younkyoo
    • Journal of the Korean Chemical Society
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    • v.62 no.5
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    • pp.364-371
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    • 2018
  • Inhibition effects of alanine (Ala), histidine (His), methionine (Met) on the corrosion of aluminum were investigated in deaerated 0.5 M HCl and NaCl solution. In HCl solution the inhibition efficiency for the aluminum corrosion depended on the cathodic inhibition, and the inhibition efficiency was increased in the order of Met$10^{-4\;}M$ the adsorption process can be explained by Langmuir isotherm, however, in the case of higher concentration by Temkin logarithmic isotherm due to the interaction between the adsorbed molecules.

Rust Fungus Diferentiation on Host Leaves (기주식물엽에 있어서 녹병균의 분화)

  • ;Rohringer R.
    • Korean Journal of Microbiology
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    • v.13 no.1
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    • pp.37-44
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    • 1975
  • As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4$ 1.0g, sucrose 30.0g, $MgSo_4$ $7H_2$O 0.5g, $FeCl_36H_2$O 1.0mg thiamine 200.gamma.g, and distilled water 1000ml, pH6.5.

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Versatilities of Calix[4]pyrrole Based Anion Receptors

  • Lee, Chang-Hee
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.768-778
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    • 2011
  • Calixpyrroles and related macrocycles are non-planer synthetic anion receptors that have attracted considerable attentions in recent years. Although the synthesis of calix[4]pyrrole (known as meso-octamethylporphyrinogen) was reported more than 100 years ago, the anion binding properties were first discovered in 1996. The simple calix[4]pyrroles can be synthesized in single step in high yield by condensation of pyrrole with acetone. The compounds showed preferential binding for halide anions including fluoride, phosphate, carboxylate, and chloride in organic media. Efforts to improve the anion affinity of calix[4]pyrrole and to enhance its selectivity have led to the synthesis of a variety of new calixpyrrole derivatives. Among the various modifications, introduction of straps on one side of the calix[4]pyrroles are the most effective. Incorporation of aromatic rings other than pyrroles also exhibited interesting binding behaviour. Introduction of signalling units as part of the strapping element enable to detect the anions on chromogenic or fluorogenic fashion. Finding of the anion transport properties across the membrane and cytotoxic effects of the calix[4]pyrroles open new window for calixpyrrole-related research. The polymer-incorporated systems have also been employed as anion complexants in solvent-solvent extraction. These old, yet easy-to-make macrocycles have well advanced more recently with the discovery of the ion-pair complexation properties. In this review, the synthetic developments and anion binding properties of calixpyrroles for the last decades will be discussed and will cover the advances in calixpyrrole chemistry.

Development of an Immobilized Adsorbent for In Situ Removal of Ammonium Ion from Animal Cell Culture Media and Its Applications to Animal Cell Culture System : II. Application to Cell Culture System (동물세포 배양액으로부터 암모늄 이온의 동시제거를 위한 고정화 흡착제의 개발과 동물세포 배양 시스템에의 응용 : II. 세포배양 시스템에의 응용)

  • 박병곤;이해익;전계택;김익환;정연호
    • KSBB Journal
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    • v.13 no.4
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    • pp.411-417
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    • 1998
  • The possibility of application of membrane type immobilized adsorbent to the fed-batch or perfusion culture system with anchorage-independent cells as well as batch system was investigated. The improvement in cell density and cell viability due to the combination of immobilized adsorbent with each culture system was evaluated for the investigation, and the optimum culture system employing immobilized adsorbent system was suggested based on the results. It was observed that the system with immobilized adsorbent showed better cell growth and cell viability than that without immobilized adsorbent in every operation system of batch, fed-batch, and perfusion. In case of batch system, 200% improvement of maximum cell density was observed in the system where ammonium chloride was added on purpose. And 50% improvement of maximum cell density was observed in the fed-batch system where ammonium ion accumulates significantly, while small increase in maximum cell density was observed in the perfusion system where dilution of waste byproducts exists. Especially, the fed-batch system showed the most significant improvement on cell growth because both compensation of nutrient and removal of ammonium ion occurred simultaneously in the system. Therefore a combined system of immobilized adsorbent and fed-batch operation could be suggested as an optimum system with in situ removal of ammonium ion.

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Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes

  • Kwon, Hyuk Woo;Kim, Seong Hwan
    • Mycobiology
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    • v.49 no.2
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    • pp.173-182
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    • 2021
  • Recently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen's interactions with the mycelium of shiitake, histopathological properties, host range, and sensitivity to diverse antifungal agents. The strain C. pseudolongus DUCC 4014 inhibited the mycelial growth of L. edodes strain (cultivar Sanjo 701ho) and caused browning in the mycelia confronted with the yeast on PDA. Spray inoculation of the yeast caused an abnormal browning symptom on the cap and/or gills of three shiitake cultivars grown on sawdust media in vinyl bags. Scanning electron microscopic images of the abnormally browned parts of shiitake fruit body illustrated that mushroom tissues were loosed and dispersed in the middle and edge of the cap and the arrangement of basidiospores borne on basidia in the gills was disturbed compared to those of normal shiitake fruit body. Spray inoculation also led to developing abnormal browning on the harvested fruit body, indicating C. pseudolongus could be a problem during mushroom storage. But the yeast was not able to induce abnormal browning on mushrooms of Pleurotus ferulae, Pleurotus fostreatus, and Agaricus bisporus. But it induced browning only on button mushroom (A. bisporus) when they were inoculated after wounding. Tests with 16 kinds of fungicides revealed that the cell growth of C. pseudolongus could be inhibited by benzalkonium chloride at MIC 7 ㎍/ml and benomyl at MIC 3 ㎍/ml.

Isolation and Characterization of Halophilic Kocuria salsicia Strains from Cheese Brine

  • Youn, Hye-Young;Seo, Kun-Ho
    • Food Science of Animal Resources
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    • v.42 no.2
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    • pp.252-265
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    • 2022
  • Kocuria salsicia can survive in extreme environments and cause infections, including catheter-related bacteremia, in humans. Here, we investigated and evaluated the characteristics of nine K. salsicia strains (KS1-KS9) isolated from cheese brine from a farmstead cheese-manufacturing plant in Korea from June to December, 2020. Staphylococcus aureus American Type Culture Collection (ATCC) 29213 was used as a positive control in the growth curve analysis and biofilm-formation assays. All K. salsicia isolates showed growth at 15% salt concentration and temperatures of 15℃, 25℃, 30℃, 37℃, and 42℃. KS6 and KS8 showed growth at 5℃, suggesting that they are potential psychrotrophs. In the biofilm-formation analysis via crystal violet staining, KS6 exhibited the highest biofilm-forming ability at various temperatures and media [phosphate buffered saline, nutrient broth (NB), and NB containing 15% sodium chloride]. At 25℃ and 30℃, KS3, KS6, and KS8 showed higher biofilm-forming ability than S. aureus ATCC 29213. The antimicrobial resistance of the isolates was evaluated using the VITEK® 2 system; most isolates were resistant to marbofloxacin and nitrofurantoin (both 9/9, 100%), followed by enrofloxacin (7/9, 77.8%). Five of the nine isolates (5/9, 55.6%) showed multidrug resistance. Our study reports the abilities of K. salsicia to grow in the presence of high salt concentrations and at relatively low temperatures, along with its multidrug resistance and tendency to form biofilms.