• 제목/요약/키워드: chitinase inhibitor

검색결과 2건 처리시간 0.014초

Chitinase Inhibitor 생산 균주의 분리와 곤충탈피 억제효과 (Isolation of Chitinase Inhibitor-producing Microorganisms and Their Inhibitory Effect on Larval-Pupal Ecdysis)

  • 성수일;김근;성낙문;강현아
    • 한국잠사곤충학회지
    • /
    • 제34권2호
    • /
    • pp.1-5
    • /
    • 1992
  • 전국에서 수집한 토양시료로부터 약 200 여종의 미생물을 분리해내고 이들 균주의 배양 산물액을 대상으로 chitinase 억제능을 조사하였다. 그 결과 누에의 중장 소화관에서 추출한 chitinase crude enzyme과 시판의 정제된 chitinase에 대하여 공통으로 효소의 활성을 억제하는 2개의 균주 S-11과 S-25를 선발하는데 성공하였다. 이들 균주의 배양 산물액을 토사기의 누에에 주사한 결과 대부분의 누에가 용화탈피에 실패하였다.

  • PDF

The role of defense-related genes and oxidative burst in the establishment of systemic acquired resistance to Xanthomonas campestris pv. vesicatoria in Capsicum annuum(oral)

  • Lee, S.C.;B.K. Hwang
    • 한국식물병리학회:학술대회논문집
    • /
    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
    • /
    • pp.64.1-64
    • /
    • 2003
  • Inoculation of primary pepper leaves with an avirulent strain of Xanthomonas campestris pv. vesicatoria induced systemic acquired resistance (SAR) in secondary leaves. This SAR response was accompanied by the systemic expression of defense-related genes, a systemic microoxidative burst generating H2O2, and the systemic induction of ion-leakage and callose deposition in the non-inoculated, secondary leaves. Some defense-related genes encoding PR-1, chitinase, peroxidase, PR10, thionin, defensin and zinc-finger protein were distiilctly induced in the systemic leaves. The systemically striking accumulation of H$_2$O$_2$and strong increase in peroxidase activity in pepper was suggested to contribute to the triggering of cell death In the systemic micro-HRs, leading to the induction of SAR. Treatment of non-inoculated, secondary leaves with diphenylene iodinium (DPI), an inhibitor of the oxidative burst, substantially reduced the induction of some defense-related genes and subsequently SAR.

  • PDF