• 한국응용과학기술학회지
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• 제35권1호
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• pp.62-69
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• 2018

건강 식품인 병아리콩을 화장품의 원료로 활용하기 위하여 Bacillus subtilis Natto 균주로 병아리콩을 발효한 낫토를 마스크 팩에 적용하여 피부개선 효과를 연구하였다. 병아리콩 낫토를 동결건조하여 얻은 분말을 paste 제형으로 얼굴 마사지 팩에 적용하였다. 50~60대 여성을 대상으로 낫토 팩의 피부개선 효과를 조사하였다. 낫토 팩의 처치 횟수가 증가함에 따라 피부가 개선되며 4회 처치 후 개선효과가 두드러졌다. 4회 처치 후 수분 함량과 피지 분비량이 각각 $8.4{\pm}3.6%p$와 $4.0{\pm}2.3%p$ 증가하였고, 피부 모공과 주름도 각각 $1.8{\pm}0.3%p$와 $1.8{\pm}0.9%p$ 감소하였다. 피부 색소침착은 $1.3{\pm}0.2%p$ 감소되었고, 피부 톤은 55.2%에서 55.9%로 $0.7{\pm}0.2%p$ 밝아졌다. 병아리콩을 발효하여 얻은 낫토 발효물이 보습, 모공, 주름, 색소침착, 피지분비, 피부톤 등의 피부개선 효과가 있으며, 다양한 기능성 화장품의 원료로 활용 가능성을 확인하였다.

• 한국식품조리과학회지
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• 제32권5호
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• pp.541-548
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• 2016

Purpose: For dissecting the possible benefits and usages of Cheonggukjang, five different beans, soybean, black soybean, red bean, chickpea, and lentil bean, underwent the fermentation process along with Bacillus subtilis. Methods: Resultant Cheonggukjang physicochemical and sensory properties such as pH, amino type nitrogen, slime contents, rheological properties, and sensory strength and consumer acceptance were analyzed. Results: Values of pH were found to be highest in Cheonggukjang made with soybean (SC), followed by Cheonggukjang made with black bean (BSC) and Cheonggukjang made with red bean and soybean mixture (RBSC), and lastly Cheonggukjang made with chickpea (CBC) and lentil bean (LBC). Amino type nitrogen values were shown to be highest in SC with a value of $394.74{\pm}28.80mg%$, subsequently were values taken from RBSC, followed by BSC, CBC, and lastly LBC. Slime contents were the highest in SC with a value of $3.44{\pm}0.36%$, with RBSC, BSC, CBC, and LBC without statistical difference. Total colony count was found to show higher value in BSC, SC, and RBSC, in contrast to the lower values found in CBC and LBC. Color was shown to be lightest in CBC and with darker shades shown that of SC, LBC, RBSC, and BSC in descending order. In rheological properties, hardness, adhesiveness, and viscosity were found to be highest in SC. Sensory strength analysis and sticky appearance were highest in SC, with fermented odor being lowest in CBC and BSC. The strength of sweetness, sourness, and umami taste did not show statistical difference, however, bitterness was shown to be highest in LBC and lowest in CBC, SC, and BSC. In the consumer acceptance test, overall acceptance was highest in SC and CBC. Conclusion: In conclusion, SC was found to be the best bean variety for making Cheonggukjang fermented with Bacillus subtilis, however, CBC may act as an alternative for manufacture of Cheonggukjang resulting in sound consumer acceptance.

• Applied Biological Chemistry
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• 제41권5호
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• pp.355-362
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• 1998

병아리콩 근류의 호스트부분에서 플라스티드에 존재하는 것으로 추정되는 포스포프룩토오스 키나아제(EC 2.7.1.11; PFK)을 순수분리 정제하고, 정제된 단백질 분자량이 220 kDa인 비당단백질(N-linked)이다. SDS-PAGE와 Western blot의 결과는 정제된 효소가 4개의 55 kDa subunit로 이루어져 있음을 지적하고 있다. 이 효소는 pH 8에서 최적활성으로 날카로운 곡선을 나타내고 있으며, 최적 pH 8과 생리적으로 비슷한 pH 7에서 Fru-6-P 및 nucleoside triphosphate 기질에 Michaelis-Menten kinetics을 나타냈다. MgATP가 뉴크레오 삼인산중에 가장 효율적인 인산기 공여체로서 나타냈다. 포스포엔롤피루베이트는 마이너 형태의 PFK 활성에 가장 강력한 억제자이며, 또한 이 효소는 3-포스포글리세레이트와 2-포스포글리세레이트에 의해 강하게 억제된다. 마이너 형태의 PFK는 KCl, NaCl등 Pi에 의해 약하게 활성이 증진되지만, 높은 농도에서는 억제자로서 작용한다.

• Advances in environmental research
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• 제3권4호
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• pp.307-319
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• 2014

Four chickpea cultivars viz. kabuli (Pusa 1088 and Pusa 1053) and desi (Pusa 1103 and Pusa 547) differing in sensitivity to high temperature conditions were analyzed in earthern pot (30 cm) at different stages of growth and development in the year of 2010 and 2011. Pusa-1053 (kabuli type) showed maximum photosynthetic rate and least by Pusa-547 (desi type), whereas maximum cell membrane thermostability were recorded in Pusa-1103 and minimum in Pusa-1088. Among the treatments, the plants grown under elevated temperature conditions had produced 13.01% more significant data in comparison to plants grown under continuous natural conditions. Stomatal conductance were reduced 44.25% under elevated temperature conditions than natural conditions, whereas 35.56%, when plants grown under initially natural conditions upto 30DAS, then 30-60DAS elevated temperature and finally shifted to natural conditions till harvest. In case of Pusa-1103, stomatal conductance was maximum as compared to rest of 2.7% from Pusa-1053, 8.9% from Pusa-1088, and 10.3% in Pusa-547 throughout the study. Plants grown under continuous elevated temperature conditions had produced 15.30% and 15.32% more significant membrane thermostability index in comparison to continuous natural conditions at vegetative stage and 19.40% and 18.44% at flowering stage, while the better response was recorded at pod formation stage. Pusa-1053 had given 2.8% more membrane thermostability index than Pusa-1088 and Pusa-1103 had given 1.6% more membrane thermostability index than Pusa-547 in the present study. The membrane disruption caused by high temperature may alter water ion and inorganic solutes movement, photosynthesis and respiration. Thus, thermostability of the cell membrane depends on the degree of the electrolyte leakage.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.52-52
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• 2020

병아리콩은 두류 중 콩, 땅콩, 강낭콩, 완두 다음으로 중요한 작물로 chickpea, garbanzo bean 이라고도 불리며, 원산지는 터키 동남부와 시리아로 알려져 있다. 병아리콩은 전세계 50개국 이상에서 재배되며, 인도에서 전세계 생산량의 66%가 재배되고 있다. 병아리콩은 Desi (microsperma)와 Kabuli (macrosperma)의 두가지 형태로 구분된다, Desi형은 꽃색이 분홍색, 종피가 두껍고 종피색이 유색이 특징, Kabuli형은 백색꽃에, 백색이나 미색 종피 양머리 종자형태를 보인다. Desi 형은 아시아와 아프리카, Kabuli 형은 서아시아, 북아프리카, 남미와 유럽 등지에서 주로 재배된다. 본 연구에서는 인도, 미얀마, 우즈벡, 조지아, 불가리아, 우크라이나 등 6개국에서 수집한 자원의 농업형질을 평가하고 비교하여 활용을 위한 정보를 제공하고자 수행하였다. 꽃색의 분포는 분홍색이 백색보다 2배 많았으며 조지아, 인도와 미얀마 수집 자원은 80% 이상이 분홍색, 우크라이나, 우즈베키스탄에서는 백색이 최소 75%이상으로 조사되었다. 소엽의 크기는 종자의 크기와 밀접한 관련이 있으며 인도와 미얀마, 불가리아에서는 소립종, 조지아, 우크라이나에서는 중립종의 비율이 높았다. 개화기는 6월 상중순에 집중되었으며 인도와 미얀마 수집자원의 개화기가 6월 상순 이전으로 빠르고 우크라이나 자원이 6월 하순과 7월 상순으로 늦은 경향을 보였다. 올해는 6월 중순부터 지속된 강우로 개화 후 정상적인 결실이 어려웠으나 6월 하순부터 성숙하기 시작하였고, 인도와 미얀마 수집자원은 주로 7월 상순에 나머지 수집 국가들은 7월 하순 이후에 성숙하였다. 올해의 기상이변으로 보다 정확한 평가를 위하여 차년도에 재평가를 수행하고자 하나 올해의 양적, 질적 형질의 경향치를 이용하여 수집국간의 농업형질을 비교하고, 식품소재의 다양화 다변화 추세에 맞추어 도입작물 활용을 위한 기초자료로 제공하고자 한다.

• 한국식품과학회지
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• 제49권6호
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• pp.638-643
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• 2017

본 연구에서는 머핀의 품질향상을 위해 두류-밀(1:1) 혼합가루로 제조한 머핀의 품질특성을 살펴보았다. 두류가루를 첨가하였을 때 밀가루만으로 제조한 대조군 머핀에 비해 명도가 감소하고 적색도가 증가하였다. 두류첨가 머핀은 대조군에 비해 상대적으로 많이 존재하는 식이섬유에 의해 높은 수분함량을 나타냈으며 조단백과 조회분의 함량도 대조군에 비해 높게 나타났다. 두류가루가 첨가된 머핀은 대조군에 비해 중량이 높았으나 높이와 부피가 감소하였고 기공의 분포도 다소 불규칙적으로 나타났다. 또한 두류가루 첨가로 인하여 머핀의 경도는 증가하였고 반대로 탄력성과 응집성이 유의적으로 감소하였다. 그러나 관능적으로 두류가루를 첨가한 머핀은 색에 대한 선호도가 대조군에 비해 높았으며 전체적인 기호도는 동부가루와 녹두가루로 제조된 머핀은 대조군에 비해 낮았으나 렌틸가루와 병아리콩가루로 제조한 머핀은 대조군과 유사한 결과를 보였다. 본 연구를 통해 두류가루를 밀가루의 50%까지 대체하여 제조한 머핀의 관능특성은 대조군과 거의 유사했고 영양성은 개선되었으며 이는 두류를 활용한 기능성 제빵 제품 개발의 기초자료로 활용될 수 있을 것으로 생각된다.

• 한국식품영양학회지
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• 제36권5호
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• pp.368-378
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• 2023

Changes in contents of free sugars, amino acids, and fatty acids of legumes were analyzed for each phase of in vitro digestion. In addition, contents of resistant starch in raw and digested pulses were compared. Soybeans, kidney beans, cowpeas, and chickpeas were analyzed. An in vitro digestion model was used to analyze contents of nutrients using LC-MS and GC-MS. Stachyose in kidneybean, cowpea, and chickpea increased as the digestion phase progressed. In four types of legumes, raffinose slightly decreased or showed no significant difference between the Oral phase and the BBMV phase. Content of glucose, a monosaccharide, increased during the BBMV phase. During the digestion phase, levels of free amino acids and free fatty acids also increased. Content of resistant starch was reduced compared to that in the raw material. It was 0.01g/100 g food in soybean, 1.06 g/100 g food in red kidney bean, 0.77g/ 100g food in cowpea, and 0.76 g/100 g food in chickpea. It was confirmed that nutrients in the in vitro digestion model were liberated at each digestion phase with changes in the content of resistant starch. These results are expected to be used as fundamental data for obtaining bioavailability of nutrients.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.966-978
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• 1993

A power operated 90.5 hp electric motor) grain flour separator was designed and developed for separation of grain (wheat, corn, chickpea and soybean) flour into various fractions based on the size of the particles of the product. The separator agitating mechanism, feed control, cylindrical separator unit and an eccentric mechanism. The machine was tested for wheat ( variety ; Sujata) flour separation into four fractions, viz ; semolina, Gr-I and II, flour (coarse) and white (fine) flour. Wheat samples (6.8% m.c., db) were first pearled by CIAE pearler for 15.8% bran removal . The pearled wheat grains were then milled for semolina by a burre mill. The product and machine characteristics were determined at different capacities varying from 24 kg/h to 143 kg/h. It was found that 76 kg/h capacity gave reasonably best results in terms of purity and recovery of semolina vis-a-vis the market product. The energy requirement of the machine at no-load was found to be 230 W and at load c nditions, it varied between 36.3-6.4 KJ per kg of fead seperation. The macine could be used by small flour millers small/medium size traders and retailers and other processors for making available various flour products of different particle size in the market for ready use of the consumers.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.979-983
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• 1993

A power operated (0.5 hp electric motor) grain flour separator was designed and developed for separation of grain (wheat, corn, chickpea and soybean) flour it no various fractions based on the size of the particles of the product. The separator is made of mild steel and consists of a hopper, power driven agitating mechanism, feed control , cylindrical separator unit and an eccentric mechanism. The machine was tested for wheat (variety : Subjata) flour separation into four fraction, viz : semolina ; Gr-I and II, flour (coarse) and white (fine) flour. Wheat samples (6.8% m.c., db) were first pearled by CIAE pearler for 15.8% bran removal . The product and machine characteristics were determined at different capacities varying from 24 kg/h to 143 kg/h. It was found that 76 kg/h capacity gave reasonably best results in terms of purity and recovery of semolina vis-a-vis the market product. The energy requirement of the machine at no-load was found to be 230 w and at load conditio s, it varied between 36.3-6.4kj per kg of feed separation. The machine could be used by small flour millers, small/medium size traders and retailers and other processors for making available various flour products of different particle size in the market for ready use fo the consumers.

• 한국미생물·생명공학회지
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• 제47권3호
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• pp.423-433
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• 2019

Midgut microbiota is known to play a fundamental role in the biology and physiology of the agricultural pest, Spodoptera litura. This study reports the difference in the larval midgut microbiota of field-caught and laboratory-reared populations of S. litura by performing 16S rDNA amplicon pyrosequencing. Field populations for the study were collected from castor crops, whereas laboratory-reared larvae were fed on a regular chickpea based diet. In total, 23 bacterial phylotypes were observed from both laboratory-reared and field-caught caterpillars. Fisher's exact test with Storey's FDR multiple test correction demonstrated that bacterial genus, Clostridium was significantly abundant (p < 0.05) in field-caught larvae of S. litura as compared to that in the laboratory-reared larvae. Similarly, bacterial genera, such as Bradyrhizobium, Burkholderia, and Fibrisoma were identified (p < 0.05) predominantly in the laboratory-reared population. The Bray-Curtis dissimilarity matrix depicted a value of 0.986, which exhibited the maximum deviation between the midgut microbiota of the laboratory-reared and field-caught populations. No significant yeast diversity was seen in the laboratory-reared caterpillars. However, two yeast strains, namely Candida rugosa and Cyberlindnera fabianii were identified by PCR amplification and molecular cloning of the internal transcribed space region in the field-caught caterpillars. These results emphasize the differential colonization of gut residents based on environmental factors and diet.