• ALGAE
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• 제28권3호
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• pp.289-296
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• 2013

For the quantitative detection of algal toxin, microcystin, a chemiluminescence immunochromatographic assay method was developed. The developed system consists of four parts, chemiluminescence assay strip (nitrocellulose membrane), horse radish peroxidase labeled microcystin monoclonal antibodies, chemiluminescence substrate (luminol and hydrogen peroxide), and luminometer. The performance of the chemiluminescence immunochromatographic assay system was compared with high performance liquid chromatography (HPLC) detection. The detection limit of chemiluminescence immunochromatographic assay system is several orders of magnitude lower than with HPLC. The chemiluminescence immunochromatography and HPLC results correlated very well with the correlation coefficient ($r^2$) of 0.979.

• 한국연소학회지
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• 제13권2호
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• pp.14-22
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• 2008

The CH* chemiluminescence of premixed flames and their dependency on fuel types has been experimentally investigated on laminar methane and propane premixed flames. The measured chemiluminescence intensities are observed linearly proportional to the fuel flow rate, which could be interpreted as the CH* chemiluminescence signal is linearly proportional to the heat release rate under fuel lean conditions. The effect of equivalence ratio could be expressed by an exponential function as ${I_{CH*}}^{\propto}\;a_1\;{\exp}(b_1{\Phi})$, where $a_1\;=\;0.00054$ and $b_1\;=\;4.60$ for methane and $a_1\;=\;0.0056$ and $b_1\;=\;5.02$ for propane. Oscillating flames showed the temporal fluctuation of chemiluminescence intensity: however, the time averaged values are virtually identical to those of quiescent flames under the same fuel flow rate and equivalence ratio conditions. This observation suggests that there is no significant flame stretch effect on chemiluminescence intensity, in average values.

• 한국연소학회지
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• 제9권3호
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• pp.19-26
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• 2004

This paper presents results of experimental study of flame chemiluminescence from turbulent premixed flames in a dump combustor. A detailed spectroscopic measurement of chemiluminescence over the wavelength of 405-495 nm is made for various flow conditions. No effect of turbulence on the relationship between chemiluminescence and heat release is found, suggesting the overall chemiluminescence intensity collected be used as a measure of overall heat release for non-oscillating stable flame. The background-$CO_2^*$ subtracted $CH^*$ chemiluminescence is found to be more sensitive to the equivalence ratio and premixedness of fuel-air mixture than $CO_2^*$ chemiluminescence.

• Current Research on Agriculture and Life Sciences
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• 제24권
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• pp.9-16
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• 2006

Ultraweak photons could be emitted from the chemical reaction without any electromagnetic radient. These chemiluminescence could be detected by highly sensible photomultiplier recently. We will introduce the principal of chemiluminescence generation reaction, and the application studies in the determination of food-borne microorganisms in field. The counts of surface contaminated microorganism in mackeral and chicken for cold storage determined by ATP-chemiluminescence method had good correlation with the counts by standard agar plate method. However, there was little correlation between 2 methods in ginseng powder whose microbial counts were lower than 10,000 CFU.

• 한국식품영양과학회지
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• 제25권2호
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• pp.193-198
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• 1996

담즙산과 결합을 하여 콜레스테롤 농도에 영향을 주는 것으로 보고된 대두 단백질의 생체내 효소에 의한 미분해성 분획물 개량 메주로부터 분리하였다. 개량 메주로부터 pH 6.8 과 pH 4.5의 분획물들을 분리하여, 초음파 처리와 투석을 행한 후 Sephadex G-75 column chromatography를 실시하여 부분 정제하였다. 부분 정제된 분획물들을 chemiluminescence 방법에 의해 in vitro에서 검색을 한 결과, pH 4.5 분획물중의 하나가 담즙산과 강하게 결합하는 것으로 밝혀졌다. 또한 대두 기원의 pepsin 비분해성 분획물 또한 pH 4.5 분획물의 chemiluminescence 결과와 일치하였다. 따라서 본 연구에서 고안한 chemiluminescence 법은 in vitro에서 담즙산 결합 활성도 측정 및 콜레스테롤 대사에 관한 연구의 기본적인 연구 수단을 평가되며 따라서 본 연구 결과는 이 분야의 연구에 기초 자료로 제공될 수 있는 가능성을 제시하였다.

• 한국연소학회지
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• 제20권4호
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• pp.1-9
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• 2015

The object of this study is a deriving the relations according to the measuring locations between the chemiluminescence and the flame state at commercial burner. In this study, the flame chemiluminescence of the flame of commercial burner is measured using a photomultiplier tube and the optical band-pass filter. In addition, the contour of the chemiluminescence of the flame is measured using the common CCD camera and the optical band-pass filters, and the acquired images is converted by the simple image processing as a matrix form. The results showed that certain relationship between optical data and equivalence ratio exists, and the contour according to the measuring location of the flame chemiluminescence is different by equivalence ratio.

• Bulletin of the Korean Chemical Society
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• 제32권1호
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• pp.149-152
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• 2011

A new chemiluminescence immunochromatographic analysis system with high sensitivity and high reproducibility was developed for the determination of microcystins (MCs) in water. Horse radish peroxidase (HRP) labeled microcystin monoclonal antibody was used for the sensitive chemiluminescence detection. The chemiluminescence immunochromatographic analysis system was composed of microcystin LR (MCLR)-monoclonal antibody (mAb)-Horse Radish Peroxidase (HRP) conjugate, MCLR-BSA conjugate, luminol, hydrogen peroxide mixture solution, an immunochromatographic assay strip and luminometer. To detect the concentration of microcystins in water, we utilized one spot analysis of the strip instead of flow type analysis. We could detect the microcystins in water at a concentration as low as 9.45 pg/mL with the chemiluminescence (CL) detection.

• 한국의류학회지
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• 제45권5호
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• pp.840-851
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• 2021

In this study, we used a chemiluminescence reaction to investigate the removal effect of pet soil, such as dog blood, urine and feces. The soiled fabrics were washed with a standard laundry course of 30℃ and a washing time of 30 min and a pet care laundry course of 40-60℃ and a washing time of 100 min. The detergency was evaluated by the surface reflectance and chemiluminescence reaction (bloodstain detection by luminol test and urine-stain and feces-stain detection by UV blacklight test) before and after washing. The surface reflectance results did not show any difference in detergency for both courses, whereas the chemiluminescence reaction did. The detergency of the pet care course compared to the standard course was 101% according to the surface reflectance and 120% according to the chemiluminescence reaction. Therefore, residual stains not detected by surface reflectance can be evaluated through chemiluminescence reaction, and it was confirmed that pet stains can be managed more hygienically by washing for a long time at a high temperature.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2014년도 제49회 KOSCO SYMPOSIUM 초록집
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• pp.219-222
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• 2014

The flame chemiluminescence is a good tracer of flame statement. In this study, the characteristics of flame chemiluminescence($OH^*$, $CH^*$, ${C_2}^*$) according different measuring locations using photomultiplier(PMT), spectrometer and CCD camera. Measurements are made for $OH^*$, $CH^*$, ${C_2}^*$ radicals in gas & light oil diffusion flames. At turbulent nonpremixed combustion mode, the equivalence ratio is varied. The experimental results showed that measuring location affects the result of flame chemiluminescence.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2012년도 제44회 KOSCO SYMPOSIUM 초록집
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• pp.185-187
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• 2012

The characteristics of stratified co-flow flames have been investigated from the flame chemiluminescence images. The fuel lean premixed flame could be stabilized with a fuel rich premixed flames that is generated with the supply of fuel through the inner nozzle. The penetration of outer region lean premixture into the fuel stream produced a lifted rich premixed flame at the center. Chemiluminescence images of OH, CH, and $C_2$ radicals indicated that the way of stratification of fuel/air mixture under various operating conditions.