• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1529-1533
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• 2008

The detection characteristics of gamma-irradiated ($0{\sim}10.0\;kGy$) medicinal herbs (Platycodon grandiflorum, Acanthopanax chiisanensis) were investigated by photostimulated luminescence (PSL), thermoluminescence (TL), and electron spin resonance (ESR). The results of the PSL, a first screening method in comparison with the TL, showed photon counts greater than 5,000 counts/60 s (positive) in the irradiated samples, while the non-irradiated samples yielded photon counts less than 700 counts/60 s (negative). The TL was also applied for the detection method of irradiated medicinal herbs and showed that the non-irradiated sample revealed a glow curve with a low intensity, while the irradiated samples showed a higher intensity. These results were normalized by re-irradiating the mineral grains with a irradiation dose of 1.0 kGy, and a second glow curve was recorded. The ratio of the intensity of the first glow curve ($TL_1$) to that after the normalization dose ($TL_2$) was determined and compared with the recommended threshold values. TL ratio ($TL_1/TL_2$) was below 0.007 for the non-irradiated sample and higher than 0.1 for all irradiated samples (above 1.0 kGy). ESR spectroscopy revealed specific signals (6.065 mT) derived from free radicals in cellulose containing irradiated medicinal herbs. The P. grandiflorum showed clearer signals than A. chiisanensis. From the results of our studies, the PSL, TL, and ESR determinations were found to be suitable for the detection of irradiated medicinal herbs such as P. grandiflorum and A. chiisanensis.

• Journal of Korean Society of Forest Science
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• v.10 no.1
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• pp.7-16
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• 1970

There are 19 species of bamboo in Korea, among which few are of commercial value. The Phyllostachys has wide range of utilization, for sea weed production, industrial production of various bamboo wares, etc. The total export from Chenla-Namdo to some more than ten countries was recorded 204,000,000 won worth in 1969. As civilization develops so does the techniques of bamboo works, and they in turn accelerate the demand for bamboo wood of better quality. In the light of this trend, the author attempted to make bamboo plants to develope a quadragular culm with elaborate design as disired instead of the original round one, with the expectation that it might be widely welcomed by hand workers, interior decorators. Here are some facts found out in this experiment: 1). The greater the diameter of the shoot at the eye level was, the better result was brought, and as shown in Fig. 5 the rate of success was 72% with the shoots of 7.5 cm thick. 2). The shoots of 30 cm in length showed 100% of success, developing into a desired quadrangular culms without fail(See Fig. 6). 3). The intensity of wood fiber increased as time lapsed, without receiving any influence by the weather (See Fig. 7, 8). 4). During the growing, the culm proved to be flexible enough to bend up to 90 degree (See Fig. 9). 5). In an attempt to promote the value of bamboo by decoration, the author tried to impress rectangular indents on the culm. The indention using a qudangular board 1cm smaller in width than the shoot diameter gave 100% success (See Fig. 10). 6). Design experiment was also successful both in coloration and impressing and the resulting designs delicately depended on the kinds of chemicals used and their combination (Table 2) (See Fig. 12, 13, 14). With the above mentioned findings, the author concludes that the mass production of quadrangular bamboo culm is quite a promissing as an new industry to develope bamboo works to a more valuable one. This research was carried out with the research fund provided by the Ministry of Education in 1969.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.799-805
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• 1998

In present paper, we examined the flayer constituents and functionality of two stage enzyme hydrolysates (TSEH) of purplish clam and oyster, and also examined reappearance of oyster flavors through repreparation of individual flayer constituents. Total free amino acid contents in TSEH was $1943.0mg\%$ for purplish clam and was $5066.2 mg\%$ for oyster, respectively, Major free amino acids in purplish clam extracts were taurine, glutamic acid, glycine, alanine, Iysine and arginine, and in oyster extracts were taurine, asparagine, glutamic acid, valine, leucine, alanine, Iysine and arginine. As for nucleotides and related compounds, AMP was the principal component though small amounts in TSEH of purplish clam and oyster, and also contents of TMAO, total creatinine, and betaine were $41.2 mg\%,\;35,9 mg\%$ and $220.9 mg\%$ for that of purplish clam and $3.51 mg\%,\;33.4 mg\%$ and $380.9 mg\%$ that of oyster, respectively. The major inorganic ions in TSEH of both samples were Na, K, P, Cl and $PO_4$, and major non-volatile organic acid was succinic acid. The TSEH of purplish clam and oyster revealed very higher inhibition effect ($84.1\%,\;77.0\%$) in ACE inhibition than that ($0\~44.7\%$) of water and autolytic extract. A synthetic oyster extract prepared from pure chemicals on the basis of the analytical data on the TSEH, satisfactorily reproduced the taste of the natural extract except for a slight lack of mildness and odor. From the omission test the major taste compounds of oyster extract were free amino acid and inorganic ions. The quaternary ammonium bases, nucleotides and related compounds seemed to net an auxiliary role in taste of that.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.347-355
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• 1986

The Sonakdong river is very important water source not only for agricultural water of Kimhae field but also for fishery water. Recently the middle and lower areas of the river have the tendency to be heavily contaminated by domestic sewage and agricultural chemicals. Fifty six water samples were collected from 8 stations from July to December in 1985 for the experiment (Fig. 1). To evaluate the water quality, pH, water temperature, electrical conductivity, chloride ion, nutrients ($NO^{-}_{2}-N,\;NO^{-}_{3}-N,\;NH^{+}_{4}-N,\;PO^{3-}_{4}-P,\;SiO_2-Si$), total coliform, fecal coliform, and fecal streptococcus were determined. Range and mean value of the samples were as follows; pH $6.3{\sim}9.4$, 7.91; water temperature $6.1{\sim}34.8^{\circ}C,\;23.88^{\circ}C$; electrical conductivity (from St. A to G) $1.575{\times}10^2{\sim}30.50{\times}10^2{\mu}{\mho}/cm,\;6.57{\times}10^2{\mu}{\mho}/cm$; chloride ion $23.5{\sim}14,300mg/l$, 770.0mg/l; nitrite-nitrogen $0.007{\sim}0.110mg/l$, 0.053mg/l; nitrate-nitrogen $0.001{\sim}1.638mg/l$, 0.649 mg/l; ammonia-nitrogen $0.017{\sim}4.200mg/l$, 0.497mg/l; phosphate-phoshorus $0.011{\sim}0.281mg/l$, 0.086mg/l; and silicate-silicious $2.4{\sim}6.5mg/l$, 4.43mg/l. Electrical conductivity and chloride ion of the station F(Chomanpo) were $2.676{\times}10^2{\mu}{\Omega}cm$ and 123.99mg/l which were lower than those of others. Among the analyzed nutrients, silicate-silicious concentration was the highest through all the samples. The bacterial density of the samples ranged $36{\sim}110,000/100ml$ for total coliform, $15{\sim}46,000/100ml$ for fecal coliform and $3.6{\sim}15,000/100ml$ for fecal streptococcus. The range and the mean of the TC/FC ratio were $3.0{\sim}9.6$, 5.51 and those of the FC/FS ratio were $1.1{\sim}9.2$, 6.19, respectively. On the other hand, fecal coliform was not detected in about $78\%$ of the water samples examined. Composition of coliform was $52\%$ Escherichia coli group, $3\%$ Citrobacter freundii group, $13\%$ Enterobacter aerogenes group and $31\%$ others.

• Journal of the Korean Society for Marine Environment & Energy
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• v.9 no.3
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• pp.160-167
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• 2006

Harpacticoid copepod Tigriopus japonicus is widely distributed in coastal waters of Korea and plays important role in marine trophic structure as a first consumer. In this study, a series of experiment were conducted to test the potential of the species as a standard test species for marine bioassay. Tolerance on salinity and pH, sensitivity on the reference materials(copper sulfate and cadmium chloride) and response on the ocean dumping materials(waste sludge) we re tested to identify if the species satisfy the basic criteria as standard species for marine bioassay. The nauplius of the species($100{\sim}200{\mu}m$) showed wide tolerance on salinity with >90.0% survival rates exposed to $5.0{\sim}35.0psu$ for 48 h. Wide adaptability on pH's were also observed from 6.3 to 8.2 with >90.0% survival rates during the test. $LC_{50}$ values for copper sulfate and cadmium chloride were $3.6{\pm}0.7ppm,\;1.7{\pm}0.8ppm$, respectively. The variations in mortality between replicates were less than 10.0%. Comparison of $LC_{50}$ values indicated that T. japonicus nauplius was lower sensitive to copper sulfate than the most marine crustaceans included copepods, however, the sensitivity of test animal to cadmium chloride higher than the adults of copepod T. japonicus, Paracalanus parvus, and marine rotifer Brachinonus plicatilis. There were significant concentration-response relationship in the mortality of T. japonicus nauplius using the elutriates of three ocean dumping materials(industrial waste sludge). 48 h $LC_{50}$ values we re $31.1{\pm}1.1%$ for the elutriate of sludge from leather processing company and $54.4{\pm}15.1%$ for that of dye production company. Based on the above experimental results, bioassay using benthic harpacticoid T. japonicus nauplius must be a good estimation tool for marine ecotoxicological assessment of waste or chemicals. Wide tolerance on the salinity and pH, and significant linear relationship between concentration and response(mortality) supported the high potential of the species as a standard test species.

• Journal of the Korean Society for Marine Environment & Energy
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• v.16 no.2
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• pp.88-101
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• 2013

The International Convention for the Control and Management of Ship's Ballast Water and Sediments was adopted at 2004 and then various BWMS (ballast water management system) have been developed. In this study, WET (whole effluent toxicity) test with algae (diatom) Skeletonema costatum as primary producer, invertebrate (rotifera) Brachionus plicatilis as 1st consumer and fish (olive flounder) Paralichthys olivaceus as predator, chemical analysis and ERA (environmental risk assessment) were conducted to assess the unacceptable effect on marine ecosystem by emitting the discharge water treated with AquaStar$^{TM}$ BWMS using electrolysis as main treatment equipment for removing the marine organisms in the ship's ballast water. The most sensitive test organism on discharge water treated with AquaStar$^{TM}$ BWMS was S. costatum that gave the NOEC value of 25.00%, LOEC value of 50.00% and 72hr-$EC_{50}$ value of 69.97% from WET test result for 20 psu salinity treated discharge water. NOEC and LOEC value of B. plicatilis and P. olivaceus exposed at 20 psu salinity treated discharge water were 50.00% and 100.00%, respectively. In the chemical analysis results, total number of substances produced by AquaStar$^{TM}$ BWMS was 18 which were bromate, 7 volatile halogenated organic compounds, 7 halogenated acetic acids, 3 halogenated acetonitriles and chloropicrin. Eighteen substances did not consider as persistence and bioaccumulative chemicals. Uncertainty of toxic property of 18 substances was high. PECs of 18 substances calculated by MAMPEC model were ranged from $4.58{\times}10^{-4}$ to $4.87{\mu}g\;L^{-1}$, PNECs of them were ranged from $1.6{\times}10^{-2}$ to $3.2{\times}10^2{\mu}g\;L^{-1}$. And, the PEC/PNEC ratio of 18 substances did not exceed 1. Therefore, ERA for produced substances indicate that the discharge water treated with AquaStar$^{TM}$ BWMS does not pose unacceptable effect on marine life. And $EC_{50}$ value of S. costatum on discharge water treated by BWMS using the electrolysis had positive correlation with initial TRO concentration, concentration and kind & level of HAAs.

• Korean Journal of Environmental Agriculture
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• v.3 no.2
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• pp.43-49
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• 1984

The acute toxicity of an insecticide cartap to several species of freshwater animals was evaluated in the laboratory with special reference to the species specificity, effects of water temperatures and pH values. The aquatic animals tested were the Carassius auratus L., Aphyocypris chinensis $G{\"{U}}NTHER$, Misgurus anguillicaudatus CANTOR, Moina macrocopa STRAUS. The susceptibility of aquatic animals to cartap was different with the species of animals. At the water temperature of $25^{\circ}C$ and pH 7, TLm values of the insecticide to the C. auratus L., A. chinensis G. and M. anguillicaudatus were 0.88, 0.26 and 0.13 ppm in 48 hours, respectively, and to Moina macrocopa S., 306 ppm in 3 hrs. In the case of the three species of fish, TLm 48 values were significantly decreased with rise in temperature. In the case of water flea, where TLm value was 107 ppm at $20^{\circ}C$, there was no consistent response to temperature change, with the highest figure at $25^{\circ}C$ than at either 20 or $30^{\circ}C$. and the susceptibility of C.auratus L. and A. chinensis G. greatly decreased with the increase of pH in water. The toxicity to M. anguillicaudatus and M. macrocopa was significantly higher at pH 9 than at pH 6 or 7. In conclusion, the toxicological reactions of the freshwater animals to cartap were variably influenced by the water temperatures and pH values of water and species of animals.

• Analytical Science and Technology
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• v.25 no.3
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• pp.178-189
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• 2012

As phthalates classified as toxic to reproduction category 2 and endocrine disrupting chemicals were more strictly regulated as Substances of Very High Concern (SVHC) for authorization in under EU REACH and considered as priority substances in RoHS II, standardization of phthalate testing method is now being proposed in IEC 62321 of IEC TC 111 and the 2nd revision of KS M 1991 is also finished. In order to assist standardization activities related to phthalating testing, solvent extraction part of existing national standards were compared and verified. Recovery of DEHP (diethylhexyl phthalate) from PVC (polyvinyl chloride) by Soxhlet extraction increased in the order of methanol, toluene, dichloromethane and hexane from 46.9% to 95.3% as measured by GC-MS. Optimum extraction time was verified to be 6 hours using hexane. Recovery of DBP (dibutyl phthalate), BBP (butylbenzyl phthalate), and DEHP from different matrixes such as PVC, nitro cellulose, ABS (acrylonitrile butadiene styrene). and EPDM(ethylene propylene diene monomer) rubber were evaluated to be more than 90% up to 99%. The detection limits of phthalates in solvent extraction followed by GC-MS analysis were 0.08~0.3 ${\mu}g/mL$ in solution and 8~30 mg/Kg in polymeric samples. GC-MS analyses of phthalates were carried out using different solvent extraction based on the EN 14372, ASTM D 7083, Japanese test method (MHLW 0906-4) and KS M 1991, proving that equivalent recoveries ranging from 98%~99% were obtained. DBP and DEHP were detected in three consumer products such as a child toy, a power cable and manicure with the amount of 22~1,910 mg/kg.

• The Korean Journal of Pesticide Science
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• v.10 no.3
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• pp.172-182
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• 2006

This study was designed to assess molinate fate in the lysimeter by measuring the total radioactivity in the leachate, evolved $^{14}CO_2$, and $^{14}C$-residues in soil and rice plant. The amounts of applied $^{14}C$ in the leachate from the lysimeter for 20 weeks were 1.05% in 2.31 pore volume (217,465 mL) at the first and 0.34% in the second year, respectively. The amount of $^{14}CO_2$ evolved from the lysimeter accounted for 6.47% and 0.03% of applied $^{14}C$ in the first and second year. The $^{14}C$-activities in the soil layer of the lysimeter were distributed 18.0% (1st) and 13.3%(2nd) in the depth of 0 to 10 cm, 4.3 (1st) and 1.1% (2nd) in the depth of 10 to 20 cm. Most of the applied $^{14}C$ was detected in the top 20 cm soil layer. Total $^{14}C$ in rice plants grown at lysimeter were detected 11.46% of applied $^{14}C$. 11.11% in straw, 0.24% in brown rice grain, 0.08% in chaff and 0.03% in ears were distributed in the first year. Consequently, environmental fate of molinate using lysimeter simulating a paddy rice field were investigated 25.24% in soil, 11.64% in rice plant, 1.05% in leachate, 6.74% in evolved $^{14}CO_2$ and 0.02% in volatilized organic chemicals in the first year.

• The Korean Journal of Pesticide Science
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• v.2 no.1
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• pp.85-90
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• 1998

A series of experiments were carried out to determine the effect of seed dressing application of chemicals on the early occurring rice insect pests. The number of small brown planthopper adults per 3hills in seed-dressing plot of Imidacloprid WS (Im WS) was 1.6 and its control efficacy was over 90%, compared with that of untreated plot. Rice water weevil was observed in the density of 8.8 larvae per 5 hills seed-dressed Im WS plots(showing 95.5% of control efficacy). Control efficacy of Im WS and Im GR against adult weevils lasted for 26 days after treatment and was higher than that of Carbofuran GR. Rates of injured stems by rice stem maggot and injured leaves by rice leaf miner were 3% and 3.7% in Im WS treatment plot respectively. Seed germination rate after seed dressing with recommended dosage (3 g/seed kg) of Im WS was 71 % on the 1st day and increased on 5th day up to that of untreated seed. The residual amount of Im in seed dressing plot was 0.11 ppm in rice roots and 0.05 ppm in leaves on the 40th day after treatment. Residual effect of Im WSI sustained for 50 days with over 95% insecticidal effect for the rice water weevil and over 90% for the brown planthopper. Quantity of the chemical applied in the field was calculated as 0.084 kg a.i./ha in seed dressing and 0.3 kg a.i./ha in seed box treatment, respectively It took 1 hour to treat insecticide by seed dressing, 2.5 hours by seed box applying, and 3.6 hours by water surface releasing per 990 $m^2$.