• 한국미생물·생명공학회지
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• 제20권1호
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• pp.34-39
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• 1992

미생물에 의해 체외로 분비하는 다당류생산 균주를 토양으로부터 분리하여 이 균주에 대한 형태학, 생리학적 성질과 화학적 동정방법을 이용하여 동정하였으며 이 균주에 의해 생성된 다당류의 물리화학적 성질을 조사하였다. 분리된 균주는 Bacillus sp.중에 Bacillus polymyxa와 아주 유사한 형태와 특성을 나타내어 Bacillus polymyxa KS-1으로 동정하였으며 생산된 다당류의 용매에 대한 용해도, 정색반응 및 적외선 흡수 spectrum을 조사한 결과 물, formic acid, formaldehyde에는 잘 용해되었으며, 다당류내 pentose, ketose 그리고 전분을 함유되지 않았으며 uronic acid가 함유되지 않음을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.644-651
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• 2013

The fermented manure derivative known as Preparation 500 is traditionally used as a field spray in biodynamic agriculture for maintaining and increasing soil fertility. This work aimed at characterizing the product from a microbiological standpoint and at assaying its bioactive properties. The approach involved molecular taxonomical characterization of the culturable microbial community; ARISA fingerprints of the total bacteria and fungal communities; chemical elemental macronutrient analysis via a combustion analyzer; activity assays for six key enzymes; bioassays for bacterial quorum sensing and chitolipooligosaccharide production; and plant hormone-like activity. The material was found to harbor a bacterial community of $2.38{\times}10^8$ CFU/g dw dominated by Gram-positives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic activities showed elevated values of ${\beta}$-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants. Quantitative details and possible modes of action are discussed.