• Applied Chemistry for Engineering
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• v.28 no.5
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• pp.581-586
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• 2017

In this study, in order to find the feasibility of thermophilic biological pre-treatment for the co-digestion of food wastewater and sewage sludge, digestion efficiency of the combined thermophilic aerobic and mesophilic anaerobic process and its effect on methane production were investigated. Also, a lab-scale co-digestion process was operated to observe parameter changes according to the increase of organic loading rates using different dilution ratios of distilled water and food wastewater (1/3 [Run I], 2/3 [Run II] in addition to using the raw food wastewater [Run III]). The results indicated that co-digestion process maintained quite stable and constant pH during entire experiments. With regard to VS removal, the higher removal was observed in the combined process and the removal efficiency was 52.24% (Run I), 66.59% (Run II) and 72.53 (Run III), respectively. In addition, the combined process showed about an 1.6-fold improved methane production rate and significantly higher methane yield than that of using single anaerobic digestion process.

• Applied Chemistry for Engineering
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• v.10 no.4
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• pp.568-575
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• 1999

Two different digestion procedures for the simultaneous determination of major and platinum-group element(PGE) in spent automobile catalysts containing PGE and silicates by inductively coupled plasma atomic emission spectrometry(ICP-AES) are compared. Combinations of mixed aids using HF, $HNO_3$, HCl, $HClO_4$, $H_2O_2$, and $H_3BO_3$ are utilized for the hot plate dissolution method and the closed-vessel microwave digestion method, The latter method has been shown to be relatively superior in terms of recovery, analysis time, and amount of aqua regia (3 parts HCl + part $HNO_3$ required to dissolve PGE in comparison with conventional open vessel hot-plate dissolution. The best results were drawn from the following conditions: In closed Teflon PFA vessels under microwave heating with temperature/pressure regulation, a 0.25 g portion of sample was digested in 2 mL of HF, 2 mL of $HNO_3$ and 6mL of HCl under the pressure of 200 psi(13.79 bar) at $180^{\circ}C$ for 1hr, followed by a second digestion stage with 16 mL of 5%(w/v) boric acid under the pressure of 20 psi(1.38 bar) at $100^{\circ}C$ for 10 min. After the microwave heating, the sample was post-treated with 10 mL of aqua regia twice by hot-plate heating. This condition gives the PGE recovery within 85~110% and the relative standard deviations within 2%. The method developed can therefore be regarded as an alternative method for routine analysis of spent automobile catalysts.

• Proceedings of the KIEE Conference
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• 2006.07c
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• pp.1629-1630
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• 2006

This paper describes a micro total analysis system ($\mu$ TAS) for detecting and digesting the target protein which includes a bead based temperature controllable microchip and computer based controllers for temperature and valve actuation. We firstly combined the temperature control function with a bead based microchip and realized the on-chip sequential reactions using two kinds of beads. The PEG-grafted bead, on which RNA aptamer was immobilized, was used for capturing and releasing the target protein. The target protein can be chosen by the type of RNA aptamer. In this paper, we used the RNA aptamer of HCV replicase. The trypsin coated bead was used for digesting the released protein prior to the matrix assisted laser desorption ionization time of flight mass spectrometer (MALDI TOF MS). Heat is applied for release of the captured protein binding on the bead, thermal denaturation and trypsin digestion. PDMS microchannel and PDMS micro pneumatic valves were also combined for the small volume liquid handling. The entire procedures for the detection and the digestion of the target protein were successfully carried out on a microchip without any other chemical treatment or off-chip handling using $20\;{\mu}l$ protein mixture within 20 min. We could acquire six matched peaks (7% sequence coverage) of HCV replicase.

• Bulletin of the Korean Chemical Society
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• v.15 no.12
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• pp.1045-1049
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• 1994

A simple and fast ion chromatographic method is developed for the determination of transition metals such as Fe, Cu, Ni, Zn and Co in atmospheric particulates. The method involves acid digestion, on-column preconcentration, and subsequent ion chromatogaphic detection. The precision of the method is less than 3${\%}$ RSD at parts per billion level for the metals studied. No significant interferences are observed. The results obtained with this method agree well with those by ICP-AES.

• Proceedings of the KSEEG Conference
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• 2005.04a
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• pp.60-63
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• 2005

• Environmental Engineering Research
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• v.19 no.1
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• pp.59-66
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• 2014

The aim of this study was to assess the effect of pre-aeration on sludge solubilization and the behaviors of nitrogen, dissolved sulfide, sulfate, and siloxane. The results of this study showed that soluble chemical oxygen demand in sewage sludge could be increased through pre-aeration. The pre-aeration process resulted in a higher methane yield compared to the anaerobic condition (blank). The pre-aeration of sewage sludge, therefore, was shown to be an effective method for enhancing the digestibility of the sewage sludge. In addition, this result confirms that the pre-aeration of sewage sludge prior to its anaerobic digestion accelerates the growth of methanogenic bacteria. Removal rates for $NH_3$-N and T-N increased simultaneously during pre-aeration, indicating simultaneous nitrification and denitrification. The siloxane concentration in sewage sludge decreased by 40% after 96 hr of pre-aeration; in contrast, the sulfide concentration in sewage sludge did not change. Therefore, pre-aeration can be employed as an efficient treatment option to achieve higher methane yield and lower siloxane concentration in sewage sludge. In addition, reduction of nitrogen loading by pre-aeration can reduce operating costs to achieve better effluent water quality in wastewater treatment plant and benefit the anaerobic process by minimizing the toxic effect of ammonia.

• Journal of Korean Society of Water and Wastewater
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• v.8 no.1
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• pp.1-8
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• 1994

Laboratory experiments were conducted to investigate the effect of digestion temperature on the settleability and dewaterability of anaerobically digested sludge. The digesters were operated at a hydraulic retention time of 20 days with a loading rate of 0.63~0.66kg volatile solids per cubic meter per day at the temperature of $35^{\circ}C$ and $55^{\circ}C$. A mixed primary and secondary municipal sludge was used as a feed. The interface height of the sludge during settling test was recorded to identify settleability. As a measure of dewaterability of the sludge, specific resistance and capillary suction time were also measured with and without chemical conditioning. Higher digestion efficiency was obtained at $55^{\circ}C$ than $35^{\circ}C$. However, the settleability and dewaterability of the sludge at $35^{\circ}C$ were quite higher than those of the sludge digested at $55^{\circ}C$. The optimum dosages of ferric chloride for sludge conditioning were 0.4% and 0.6% at $35^{\circ}C$ and $55^{\circ}C$, respectively. The filtrate COD of the sludge digested at $55^{\circ}C$ was higher than at $35^{\circ}C$, which means that poor dewaterability of the sludge result in high filtrate COD.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.10 no.S_1
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• pp.35-40
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• 2001

Effect of the addition of an enzyme/microbial additive(EMA) to enhance anaerobic digestion of the primary sludge was investigated. Two laboratory scale anaerobic digester were operated with primary sludge taken from a municipal wastewater treatment plant. The digester receiving EMA with the sludge feed performed better than the control digester, when both were operated at 10-days and 15-days Solid Retention Time(SRT). Addition of EMA to the experimental digester provided 7%(10-days SRT) and 16%(15-days SRT) higher gas production compared to the control digester when both were fed with the same amount of volatile solids. The reduction in volatile solids was 24% better in the experimental digester compared to the control ar 10-days SRT, and the improvement 10% at 15-day SRT. Improvement in COD reduction, and fecal coliform density reduction were also seen in the experimental digester due to EMA addition compared to the control both ar 10-days SRT and 15-day SRT operation. Preliminary cost benefit analysis for a wastewater treatment plant showed that approximately $115/day in gas production improvements can be realized upon addition of EMA to primary sludge anaerobic digesters operating at 10-day SRT. The value of increased gas production was $172/day if the same digesters are operated with EMA addition at 15-day SRT.

• Journal of Environmental Health Sciences
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• v.14 no.1
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• pp.63-71
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• 1988

This study is an experimental research on the anaerobic digestion of pig manure and night soil mixed waste at room temperature (25$\circ$C), and the results are shown below: 1. The steady-state condition based on gas production as digestion temperature dropped to 25$\circ$C from 35$\circ$C was achieved at around 28, 47, 56, 64 days respectively when its hydraulic retention time(HRT) are 10, 20, 30, 40 days. 2, Alkalinity and volatile acid(VA) was increased as increasing the organic loading. 3. Removal efficiency of chemical oxygen demand(COD) and biochemical oxygen demand (BOD) was improved as longer HRT, and generally COD value is lower and BOD value is higher relatively. 4. Overall treatment efficiencies of mixed waste are higher than of pig manure and of night soft. 5. Organic removal efficiency at room temperature (25$\circ$C) is 20-25% lower at medium temperature (35$\circ$C) in a same VS loading condition. 6. Refractory fraction of the infiuent VS and organic removal rate constant(K) estimated at around 37% and 0.107/day respectively.

• Parasites, Hosts and Diseases
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• v.42 no.2
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• pp.57-60
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• 2004

A highly specific antigenic protein of 31 kDa from plerocercoid of Spirometra mansoni (sparganum) was obtained by gelatin affinity and Mono Q anion-exchange column chromatography. The purified 31 kDa protein was subjected to N-glycan enzymatic digestion for structural analysis. The relative electrophoretic mobility was analyzed by SDS-PAGE, before and after digestion. On SDS-PAGE after enzymatic digestion, the 31 kDa protein showed a molecular shift of approximately 2 kDa, which indicated the possession of complex N-linked oligosaccharides (N-glycosidase F sensitive) but not of high-mannose oligosaccharides (endo-beta-N-acetylglucosaminidase H, non-sensitive). Chemically periodated 31 kDa protein showed statistically non-significant changes with human sparganosis sera by enzyme linked immunosorbent assay (ELISA). Therefore, the dominant epitopes of the 31 kDa molecule in human sparganosis were found to be mainly polypeptide, while N-glycans of the antigenic molecule in sparganum was minimal in anti-carbohydrate antibody production.