• Animal Systematics, Evolution and Diversity
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• v.24 no.1
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• pp.25-32
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• 2008

The Gold-spotted pond frog, Rana plancyi chosenica, designated as a vulnerable species by IUCN Red list. This species is a typical example facing local population threats and extinction due to human activities in South Korea. A strategic conservation plan for this endangered species is urgently needed. In order to provide information for future conservation planning, accurate information on the genetic diversity and taxonomic status is needed for the establishment of conservation units for this species. In this study, we used a molecular genetic approach using the mitochondrial cytochrome b gene and control region sequences to find the genetic diversity of gold-spotted pond frogs within South Korea. We sequenced the mitochondrial DNA cytochrome b gene and control region of 77 individuals from 11 populations in South Korea, and one from Chongqing, China. A total of 15 cytochrome b gene haplotypes and 34 control region haplotypes were identified from Korean gold-spotted pond frogs. Mean sequence diversity among Korean gold-spotted pond frogs was 0.31% (0.0-0.8%) and 0.51% (0.0-1.0%), respectively. Most Korean populations had at least one unique haplotype for each locus. The Taean, Ansan and Cheongwon populations had no haplotypes shared with other populations. There was a sequence divergence between Korean and Chinese gold-spotted pond frogs (1.3% for cyt b; 2.9% for control region). Analysis of genetic distances and phylogenetic trees based on both cytochrome b and control region sequences indicate that the Korean gold-spotted pond frog are genetically differentiated from those in China.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.1
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• pp.1-9
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• 2008

The Logistic cone is studied as a most desirable for the software testing effort. Assuming that the error detection rate to the amount of testing effort spent during the testing phase is proportional to the current error content, a software-reliability growth model is formulated by a nonhomogeneous Poisson process. Using this model the method of data analysis for software reliability measurement is developed. After defining a software reliability, This paper discusses the relations between testing time and reliability and between duration following failure fixing and reliability are studied SRGM in several literatures has used the exponential curve, Railleigh curve or Weibull cure as an amount of testing effort during software testing phase. However, it might not be appropriate to represent the consumption curve for testing effort by one of already proposed curves in some software development environments. Therefore, this paper shows that a logistic testing- effort function can be adequately expressed as a software development/testing effort curve and that it gives a good predictive capability based on real failure data.

• Journal of Periodontal and Implant Science
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• v.40 no.3
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• pp.111-118
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• 2010

Purpose: The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking. Methods: In step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified vitrification formulae named T1 (75% vitrification media + 25% F-media), T2 (50% vitrification media + 50% F-media) and T3 (25% vitrification media + 75% F-media) were used to store PDL cells for 2 weeks and 4 weeks in liquid nitrogen. MTT assay was performed to examine the viability of PDL cells. Results: Maximum cell viability was achieved in cells stored in 100% conventional cryopreservation media at $-196^{\circ}C$ (positive control group) in step 1. Compared to the positive control group, viability of the cells stored in 100% vitrification media was very low as 10% in all test conditions. In step 2, as the percentage of vitrification media decreased, the cell viability increased in cells stored for 2 weeks. In 4-week storage of cells in step 2, higher cell viability was observed in the T2 group than the other vitrification formulae while the positive control group had the highest viability. There was no statistically significant difference in the cell viability of 2-week and 4-week stored cells in the T2 group. Conclusions: These observations indicate 100% vitrification media is not successful in PDL cell cryopreservation. Conventional cryopreservation media is currently the most appropriate media type for this purpose while T2 media would be interesting to test for long-term storage of PDL cells.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.3
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• pp.223-232
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• 2020

Sesamin, a lipid-soluble lignin originally isolated from sesame seeds, which induces cancer cell apoptosis and autophagy. In the present study, has been reported that sesamin induces apoptosis via several pathways in human lung cancer cells. However, whether mitophagy is involved in sesamin induced lung cancer cell apotosis remains unclear. This study, the anticancer activity of sesamin in lung cancer was studied by reactive oxygen species (ROS) and mitophagy. A549 cells were treated with sesamin, and cell viability, migration ability, and cell cycle were assessed using the CCK8 assay, scratch-wound test, and flow cytometry, respectively. ROS levels, mitochondrial membrane potential, and apoptosis were examined by flow cytometric detection of DCFH-DA fluorescence and by using JC-1 and TUNEL assays. The results indicated that sesamin treatment inhibited the cell viability and migration ability of A549 cells and induced G₀/G₁ phase arrest. Furthermore, sesamin induced an increase in ROS levels, a reduction in mitochondrial membrane potential, and apoptosis accompanied by an increase in cleaved caspase-3 and cleaved caspase-9. Additionally, sesamin triggered mitophagy and increased the expression of PINK1 and translocation of Parkin from the cytoplasm to the mitochondria. However, the antioxidant N-acetyl-L-cysteine clearly reduced the oxidative stress and mitophagy induced by sesamin. Furthermore, we found that cyclosporine A (an inhibitor of mitophagy) decreased the inhibitory effect of sesamin on A549 cell viability. Collectively, our data indicate that sesamin exerts lethal effects on lung cancer cells through the induction of ROS-mediated mitophagy and mitochondrial apoptosis.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.32 no.3
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• pp.266-272
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• 1996

The distribution and abundance of fish in the Cheju southeastern area was studied by the combined hydroacoustic and bottom trawl surveys in April 1994 and in July 1995, respectively. The main purpose of these investigations was to provide the basic data for this management and the biomass estimation of commercially important demersal fish stocks in this area. The hydroacoustic surveys were performed by using a 50 kHz scientific echo sounder system with a microcomputer-based echo integrator. Acoustical measurements of fish abundance and distribution were conducted along the cruise tracks of research vessel and during all trawl hauls by continuous echo sounding. The average weight-normalized target strength for demersal fish aggregations was derived from the relationship between the mean volume backscattering strength for the depth strata of trawl hauls and the weight per cubic meter of trawl catches. The geographical distribution of fish stocks in the 1994 survey area was investigated in relation to oceanographic conditions. The results obtained can be summarized as follows: 1. From the 1994 and 1995 survey data, the relationship between the mean volume backscattering strength (, dB) for the depth strata of trawl hauls and the weight (W, kg/$m^3$) per cubic meter of trawl catches was expressed by the following equation = - 32.8+ lOlog(W) The average weighted-target strength value at 50 kHz derived from this equation was .. 32.8 dB/kg. 2. In 1994 and 1995, both surveys showed a trend of decreasing fish abundance toward the southern area of the Cheju Island with high densities offish along the west coast ofth.e Tsushima Island. The highest demersal concentrations in the southern area of the CheJu Island appeared in bottom waters colder than $12^{\circ}C.$. . 3. From the results of combined bottom trawl and hydroacoustic surveys, the estunated fish densities in the southeastern area of the Cheju Island were 1.5488 x $10^-4$kg/$m^3$ in the 1994 surveyand 1.9498 x $10^-4$kg/$m^3$ in the 1995 survey, respectively.

• Korean Journal of Acupuncture
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• v.19 no.1
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• pp.25-33
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• 2002

Substantial evidence suggests that behavioral and reinforcing effects of cocaine can be mediated by the mesolimbic dopaminergic system. Injections of cocaine can produce one of the immediate-early gene, c-fos expression in the brain and behavioral activation. Acupuncture as a therapeutic intervention is widely used for the treatment of many mental disorders such as drugs of abuse. In order to investigate whether acupuncture has an influence on cocaine-induced reinforcing and behavioral effects, we examined the effect of acupuncture on cocaine-induced locomotor activity and c-Fos expression in the nucleus accumbens and the striatum using Fos-like-immunoreactivity(FLI). Male SD rats received acupuncture for 1 min after injection of cocaine hydrochloride(1 mg/kg, i.v.). The employed acupuncture point, Shenmen$(HT_7)$, has been clinically used to treat mental and psychosomatic disorders. Injections of cocaine produced a marked increase in locomotor activity and FLI in the nucleus accumbens and the striatum. Acupuncture at $HT_7$, but not at control points($PC_6,\;TE_4$ or tail), significantly attenuated cocaine-induced increase in locomotor activity and Fos-like immunoreactivity. These results demonstrated that reduction in locomotor activity by acupuncture may be reflected by reduction of postsynaptic neuronal activity in the nucleus accumbens and the striatum. Our results suggest that acupuncture may have a therapeutic effect on cocaine addiction.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.113-118
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• 2006

Recently, wireless LANs are often applied in home or office because of its various of convenience. Frequency rage of wireless LANs specified by IEEE 802.11b is at 2.4 GHz. The bluetooth, the microwave oven, and the PDA(Personal Digital Assistants) uses ,however, the same frequency band. So problems will be produced in these environments, such as multi-pass interference and system-to-system interference. These problems can be eliminated by using EM wave absorber. In this paper, we designed and fabricated EM wave absorber using Sendust, $Al(OH)_3$, and CPE. The double-layered EM wave absorber with thickness of 1.5 mm has absorption ability more than 10 dB at 2.4 GHz.

• Development and Reproduction
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• v.24 no.4
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• pp.287-296
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• 2020

The absence of functional estrogen receptor α (Esr1) results in an overgrowth of the epididymal fat, as observed in estrogen receptor α knockout (ERαKO) mouse. The present research was aimed to evaluate expression of various molecules associated with adipocyte differentiation and maturation in the epididymal fat of ERαKO mouse at several postnatal ages by using quantitative real-time polymerase chain reaction. The highest transcript levels of all molecules were detected at 12 months of postnatal age, except leptin which the mRNA level was increased at 5 months of age and was unchanged until 12 months of age. The expression levels of CCAAT enhancer binding protein (Cebp) alpha, androgen receptor, and lipoprotein lipase were decreased at 5 months of age but increased at about 8 months of age. The mRNA levels of Cebp gamma and sterol regulatory element binding transcription factor 1 remained steady until 8 months of age. Continuous increases of transcript levels during postnatal period were found in Cebp beta, estrogen receptor (ER) beta, fatty acid binding protein 4, and delta like non-canonical Notch ligand 1. The increases of peroxisome proliferator-activated receptor gamma and adiponectin mRNA levels were detected as early as 8 months of age. The levels of fatty acid synthase and resistin transcript at 5 and 8 months of age were lower than that at 2 months of age. These findings show the aberrant expression patterns of genes related to adipocyte differentiation and maturation in the postnatal epididymal fat pad by the disruption of ER alpha function.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.6
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• pp.129-136
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• 2008

The purpose of this study is to find an effective operating system for water supply of Andong Dam. The reliability of water supply was assessed by HEC-5 model based on operated water supply data of Andong Dam and data of Jin-dong water level gauge station in Nak- dong river. In addition, estimated additional amount of water supply was evaluated for each alternative by additional retention of Andong Dam in rainy season from June to September. As the result, additional amount of water supply of each alternative in non-rainy season (excluding rainy season) is increased as $1.35m^3/s{\sim}2.12m^3/s$, it shows that additional amount of water supply can be made by effective operating system for water supply in every dam as Andang Dam.

• Korean Journal of Social Welfare
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• v.61 no.3
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• pp.55-76
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• 2009

This study tested the psychometric properties of the Korean version of the Capacity-to-Consent Screen: K-CCS) scale. A total of 404 South Korean older adults were e Cruited using the purposive sampling method. The participants were asked to res-Cod to a structured questiConaire which included older adults' characteristics, ADLs, IADLs, cognitive impairments and creen: K-CCS) scale. A total of. Item analysis, explonalory factor analysis(EFA), and ) sfirmalory factor analysis(CFA) were cCoducted to verifK-Che psychometric properties of the Korean Version of CCS. The sample was divided into two groups: one group for EFA(n=202) alysis(Eois(r group for CFA(n=202). The totults revealeysisae. he final version of the 8-item K-CCS with two dimensions had a excellent internof. Itemstencyonofpha value=.f) alysa CFA) sfirmed the acceptaas diviof the modef.fdi(RMSEA=.057, NNFI=.f4, IFI=.f6, CFI=.f6) to twaddition, claims for the convergent and criterion-related validdiviwere demItetnaled. Ity-tnclusion, the K-CCS can be rmed for professi sams to asersioolder participants' capacity to consent to clinical or survey research.