• Journal of Microbiology and Biotechnology
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• 제24권10호
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• pp.1319-1326
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• 2014

Rapamycin, produced by the soil bacterium Streptomyces hygroscopicus, has the ability to suppress the immune system and is used as an antifungal, anti-inflammatory, antitumor, and immunosuppressive agent. In an attempt to increase the productivity of rapamycin, mutagenesis of wild-type Streptomyces hygroscopicus was performed using ultraviolet radiation, and the medium composition was optimized using glycerol (which is one of the cheapest starting substrates) by applying Plackett-Burman design and response surface methodology. Plackett-Burman design was used to analyze 14 medium constituents: M100 (maltodextrin), glycerol, soybean meal, soytone, yeast extract, $(NH_4)_2SO_4$, $\small{L}$-lysine, $KH_2PO_4$, $K_2HPO_4$, NaCl, $FeSO_4{cdot}7H_2O$, $CaCO_3$, 2-(N-morpholino) ethanesulfonic acid, and the initial pH level. Glycerol, soytone, yeast extract, and $CaCO_3$ were analyzed to evaluate their effect on rapamycin production. The individual and interaction effects of the four selected variables were determined by Box-Behnken design, suggesting $CaCO_3$, soytone, and yeast extract have negative effects, but glycerol was a positive factor to determine rapamycin productivity. Medium optimization using statistical design resulted in a 45% ($220.7{\pm}5.7mg/l$) increase in rapamycin production for the Streptomyces hygroscopicus mutant, compared with the unoptimized production medium ($151.9{\pm}22.6mg/l$), and nearly 588% compared with wild-type Streptomyces hygroscopicus ($37.5{\pm}2.8mg/l$). The change in pH showed that $CaCO_3$ is a critical and negative factor for rapamycin production.

• Nutritional Sciences
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• 제9권2호
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• pp.124-130
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• 2006

To examine the effects of alcohol consumption on body fluid restoration and fat mobilization following exercise induced dehydration, nine healthy collegiate men ($24{\pm}2yrs,\;177{\pm}5cm,\;72{\pm}8kg,\;10.5{\pm}2.3%$ body fat) underwent three experiments. In each experiment, subjects ran on a treadmill to reduce individual body mass to $2.2{\pm}0.1%$ and consumed one of three beverages containing 0, 4, or 8% alcohol over 60 min followed by 4 hr of resting recovery. They consumed approximately 150% of weight loss $(2053{\pm}204,\;2091{\pm}149,\;and\;1943{\pm}295mL)$ and content of alcohol was $9.9{\pm}1.0(0%),\;71.9{\pm}5.1(4%)$, and $132.2{\pm}20.1g$ (8% trial). Body weight, urine volume and samples, blood samples, and thirst sensation were measured five times; at baseline, immediately after exercise, and 0, 1st, and 4th hr of recovery. Blood alcohol concentration after ingestion was $0.0{\pm}0.0(0%),\;0.1{\pm}0.02(4%)$, and $0.2{\pm}0.03%$ (8% trial). No differences in blood sodium and potassium concentrations, and urine specific gravity were noticed over time periods and trials. Thirst sensation tended to be elevated in all trials immediately after exercises and urine output was elevated during the recovery. The magnitude of changes in these variables was proportional to the alcohol concentrations, but not statistically significant. While serum osmolality was not different among trials and time periods in 0 and 4% trials, it was higher during recovery than the baseline in the 8% trial (P<0.01). Triglycerides did not change throughout the time period and among trials. Free fatty acids were elevated after exercise in all trials and 4th hr of recovery in 0% (P<0.05). Subjects' net body fluid balance at 4th hr of recovery was negatively maintained and proportional to alcohol concentrations. Only 8% trials showed a significant reduction at 1st and 4th hr of recovery compared to 0 hr. The results suggested that diuretic effect of alcohol after moderate level of dehydration appeared dose dependent, but beverage containing alcohol up to 4% did not induce impaired rehydration than alcohol free drinks. Alcohol effects on fat mobilization during recovery appeared to be minimal and the mechanism is unclear.

• 한국전산구조공학회논문집
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• 제27권3호
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• pp.199-206
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• 2014

본 논문에서는 변단면 기능경사재료 보에서 중립면 탄성계수가 축방향을 따라 공간적 불확실성을 가질 경우에 대한 구조 응답변화도 산정을 위한 정식화에 대해 논한다. 기능경사재료는 두 이질재료의 체적비가 두께방향으로 연속적으로 변하며 고체화되는 과정으로 제작되는 재료로서 온도 및 응력 등에서 연속적인 변화를 가능하게 하여, 전통 복합재료에서 나타나는 층분리나 균열 발생 등이 제거되는 장점을 가지고 있다. 그러나 이론적으로 설정된 기능경사에 맞는 재료의 제작이 어려우며, 이에 따라 내재적인 불확실성을 가지고 있다. 이를 모사하기 위하여 중립면 탄성계수에서의 불확실성을 추계장으로 모델링하고, 추계적분에 의한 확률변수를 도입하여, 변위의 1, 2차모멘트를 산정할 수 있는 방법을 제시하였다. 제안된 해석 방법은 스펙트럼모사법을 적용한 몬테카를로 해석으로 검증하였다. 추계장의 상관관계거리에 따른 분산계수의 변화, 재료지수 및 기하인수가 변위의 분산계수에 미치는 영향 등을 고찰하였고, 몬테카를로 해석 대비 제안 해석법의 효율성에 대해서도 논하였다.

• 한국보건간호학회지
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• 제18권1호
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• pp.80-89
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• 2004

This study focused on individual health education for people with hypertension who were being seen in public health centers. The program was an adjusted intervention program that considered the characteristics of each individual and reflected the individual's will to change. Each individual had a different lifestyle with different characteristics and habits so the study considered these individual's characteristics. A comparison was done of knowledge related to hypertension, self-efficacy, and self-care in these individuals. In order to determine the effectiveness of education given by the nurses the variables were measured before and after the individual health education program. The participants in this study were 85 people with hypertension who were seen at a public health center in G city. They were assigned to an experimental group (43) and a control group (42). A tool developed by Park Young-Im (1994) was used to measure knowledge related to hypertension and self-efficacy. A tool developed by Lee Young-Whee (1994) was used for self-care. Data collection was done for 10 weeks from March 24 to May 31, 2003 using interviews with questionnaires. The following is a summary of the results of the study. Hypothesis 1, 'the experimental group which had the individual health education will have higher knowledge scores on hypertension than the control group that did not have the individual health education' was supported (t=4.17, P=0.00). Hypothesis 2, 'the experimental group which had the individual health education will have higher self-efficacy scores than the control group that did not have the individual health education' was supported (t=4.06, P=0.00). Hypothesis 3, 'the experimental group which had the individual health education will have better self-care ability than the control group that did not have the individual health education' was supported (t=4.94, P=0.00). Based on the results of this study, the public health centers should develop an educational program that uses a variety of visual aids and assess the effects of the education on patients with different chronic diseases. After the development of an inclusive education program which will standardize health education effectively and provide a variety of teaching methods, study is needed to measure changes in lifestyle after education and to determine how much knowledge related to hypertension, self-efficacy, and self-care increase

• 유통과학연구
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• 제13권1호
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• pp.35-46
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• 2015

Purpose - This paper attempted to investigate the relationships among the perception of job insecurity and organizational effectiveness, and it examines these relationships while considering the moderating effect of trust in the manager and self-efficacy. Job insecurity is an independent variable, organizational effectiveness aspects (job satisfaction, organizational commitment, and turnover intention) are dependent variables, and trust and self-efficacy are moderators. Research design, data, and methodology - Regression analysis was used to verify the effects. The measurement items were already deemed as reliable and valid in the previous study, but for this purpose, some modifications were made. To examine the model, this study relied on the samples chosen from Korean employees in the six franchise business firms. The survey was conducted on 288 workers. Each question is based on a 5-point Likert type scale. The data were analyzed using SPSS 18.0. Results - The results of the study are summarized as follows. First, job insecurity has an influence on organizational effectiveness aspects (negatively on job satisfaction, negatively on organizational commitment, and positively on turnover intention). Second, analyzing the moderating effect of trust, trust in manager is mediated between job insecurity and organizational effectiveness aspects (on organizational commitment and on turnover intention). However, employees' trust in manager had no significant moderating effect on the job insecurity-job satisfaction relationship. Third, self-efficacy is mediated between job insecurity and organizational effectiveness aspects (on job satisfaction, on organizational commitment, and on turnover intention). Conclusions - First, it is necessary to be aware of the seriousness of employees' job insecurity in franchise firms, which have played a pivotal role in national economic growth, and to create a detailed plan for reducing insecurity and actively implementing it. To this end, the franchise companies should implement efficiency efforts such as obtaining an appropriate capacity of equipped personnel and a training program. Second, there are moderating effects of self-efficacy and trust in the relationship between job insecurity and organizational effectiveness. Depending on business conditions, stability and instability can only be experienced in the organizations of franchise companies. This can give rise to instability in employment. Therefore, it is necessary to develop and utilize a system that can be minimized with a change in the new awareness of employment instability. After all, a good leader (manager) must accumulate personal trust and build up a core competence that is necessary to become competent in the field himself. Even if you lack the material resources to improve performance, if the leader with the core competencies (e.g., technical/management /organization/marketing/design) can gain the trust of the supervisor, you can get a good organizational performance. Therefore, you should foster a healthy organizational culture through education such as leadership training and employee training to be built on trust between the leaders and the employees. In addition, you need to focus on HRD&M training in order to increase the self-efficacy of the supervisor.

• 사회복지연구
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• 제49권4호
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• pp.101-129
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• 2018

본 연구는 폭력감소에 대한 부부폭력 가해자프로그램의 전체효과를 확인하였다. 국내에서 2000년부터 2017년까지 발표된 11편 논문을 대상으로 18개 연구결과와 115개의 효과크기를 산출하였다. 가해자프로그램은 폭력행동 변화에 실질적이고 치료적인 차원에서 유의미하게 효과적인 것으로 나타났다. 효과크기는 통제행동, 정서적 폭력, 신체적 폭력 순으로 높았다. 폭력성 이외의 다른 종속변인들의 효과를 조사해 보았다. 가해자프로그램은 내담자들의 성역할과 폭력에 대한 태도를 긍정적으로 변화시키고 있었다. 또한 분노와 우울 수준을 낮추고, 자존감과 의사소통기술을 향상시키며 부부관계만족도를 높이는 것으로 확인되었다. 이 가운데 성역할 태도와 자존감이 상대적으로 큰 효과크기를 보였다. 나아가 이 연구는 폭력행동을 감소시키는 가해자프로그램의 효과에 영향을 주는 요인이 있는지 살펴보았다. 분석결과 접근모델과 상담형태의 조절효과가 확인되었다. 구체적으로, 여성주의 인지행동 모델이 절충모델이나 단일모델보다 효과가 더 있는 것으로 밝혀졌다. 상담형태에서는 가해남성으로만 구성된 집단상담이 부부집단상담에 비해 효과크기가 더 크게 나타났다. 이러한 결과를 바탕으로 부부폭력 가해자프로그램의 효과를 높이기 위한 정책적, 실천적 방안을 논의하였다.

• 청정기술
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• 제24권4호
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• pp.332-338
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• 2018

현재 리튬이온전지의 음극 소재 활물질로는 흑연이 주로 사용되고 있다. 그러나 흑연의 최대 이론 용량이 $372mA\;h\;g^{-1}$으로 제한되기 때문에 차세대 고용량 및 고에너지 밀도의 리튬이온전지 개발을 위해서는 새로운 음극 소재 활물질이 필요하다. 여러 음극 소재 활물질 중에서 Si의 최대 이론 용량은 $4200mA\;h\;g^{-1}$으로 흑연의 최대 이론 용량보다 약 10배 이상 높은 값을 나타내고 있지만 부피 팽창율이 거의 400%로 크기 때문에 사이클이 진행될수록 비가역 용량이 증가하여 충전 대비 방전 용량이 현저히 감소하는 현상을 나타내고 있다. 이러한 문제점을 해결하기 위한 방법으로 Si 음극 소재 활물질의 입자 크기를 조절하여 기계적 응력 및 반응상의 체적 변화를 감소시켜 사이클 특성을 다소 향상시킬 수 있다. 따라서 Si 입자의 부피 팽창율에 따른 충전 및 방전 용량의 감소를 최소화하기 위해 공정 시간 및 원가 절감이 우수한 건식 방법으로 Si을 분쇄하여 사이클 특성 향상에 관한 연구를 진행 하였다. 본 논문에서는 진동밀을 이용하여 Si을 나노 크기로 제어하고 실험 변수에 따른 재료들의 물리화학적 특성과 전기화학적 특성을 측정하였다.

• 한국지리정보학회지
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• 제23권4호
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• pp.52-67
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• 2020

최근 북극은 매년 영구 동토층이 녹아 눈으로 덮인 땅이 드러나고 있어 해당 지역 관리를 위한 공간정보가 필요하다. 한국의 국토지리정보원(NGII)은 극지방의 공간정보를 구축하여 극지공간정보 서비스를 제공하고 있으나, 식생 정보는 제공되지 않고 있으므로 식생 공간정보 구축을 위한 추가적인 연구가 필요하다. 본 연구에서는 북극 스발바르제도의 뉘올레순 지역에 대한 식생 분류를 수행하기 위해 다중 시기의 Sentinel-2 영상을 사용하였다. 전처리 단계에서는 다중 시기 Sentinel-2 영상으로부터 10개 밴드와 6가지 정규 지수식을 생성하였다. 영상 분류는 8개 속성에 대한 토지피복분류를 통해 전체 식생 영역을 추출하는 과정과 전체 식생 영역 내에서 다시 세분류를 수행하는 과정으로 이루어졌다. 영상 분류 알고리즘은 OOB(Out-Of-Bag)를 통해 정확도 평가 및 변수 중요도를 산정할 수 있는 랜덤포레스트를 사용하였다. 전체 정확도는 다시기 영상이 사용되었을 경우와 식생 지수가 추가되었을 경우의 이점을 확인하기 위해 사용된 영상 수에 따라 각각 정확도를 산정하였다. 단일시기의 Sentinel-2 영상은 전체 정확도가 77%였으나, 7개의 다중 시기 Sentinel-2 영상을 기반으로 학습하였을 때, 81%로 향상되었다. 또한, 식생 지수가 추가로 사용된 학습에서 전체 정확도가 약 83%로 향상되었다. 식생 분류 시 변수 중요도는 적색, 녹색, 단파적외선-1 밴드가 가장 높은 변수로 선정되었다. 본 연구는 극지방의 식생에 대한 분류를 수행할 시 입력특성을 최적화하는 기초 연구로 활용될 수 있을 것으로 판단된다.

• 운동영양학회지
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• 제24권4호
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• pp.34-43
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• 2020

[Purpose] The purpose of this study was to assess changes in blood glucose level, blood pressure, and arterial stiffness after a 75 g oral glucose tolerance test (OGTT) in elderly women aged over 65 years with hypertension and either normal glycemic control, impaired fasting glucose tolerance, or diabetes mellitus. We also wished to investigate the relationship between stages of diabetes and physical fitness. [Methods] A total of 24 elderly women with hypertension were assigned to a control group (CON; n=7), impaired fasting glucose group (IFG; n=9), and diabetes mellitus group (DM; n=8). In each group, blood glucose level, brachial ankle pulse wave velocity (PWV), and blood pressure were measured at baseline as well as 60 and 120 minutes after a 75 g OGTT. Physical fitness factors such as hand grip strength, balance test, 4 m gait speed test, chair stand test, short physical performance battery, and 6-minute walking test were subsequently assessed. [Results] In all three groups, blood glucose levels were significantly increased at 60 and 120 minutes after a 75 g OGTT. In the DM group, blood glucose levels were significantly higher before and after a 75 g OGTT than in the CON group. In the CON group, PWV was significantly increased at 60 minutes after a 75 g OGTT; however, there were no changes in other groups after glucose ingestion. In the CON group, systolic and diastolic blood pressures were significantly decreased at 60 and 120 minutes after a 75 g OGTT compared to baseline. However, there was no change in blood pressure after ingestion in the DM group. The IFG group had greater grip strength than the CON group; however, there were no differences in other variables between the groups. [Conclusion] After a 75 g OGTT, elderly women with hypertension and diabetes maintain higher blood glucose levels compared to those with hypertension alone. Unlike elderly women with hypertension alone, those with hypertension and diabetes did not show changes in arterial stiffness and blood pressure after a 75 g OGTT. Therefore, elderly women with hypertension and diabetes may not be able to control their blood vessels following a 75 g OGTT due to impaired vascular endothelial function. Moreover, there was no association between diabetes stage and physical fitness in elderly women with hypertension.

• 한국노년학
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• 제29권2호
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• pp.729-742
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• 2009

본 연구는 2003년부터 2년마다 실시되고 있는 종단연구 자료를 바탕으로 노년기의 정서 경험의 변화 특성을 확인하고 이러한 특성이 주관적 안녕감에 미치는 영향을 알아보고자 실시되었다. 2003년 기준으로 65세 이상인 노인 중 1차(2003년), 2차(2005년), 3차(2007년) 연구에 모두 참여한 노인 참가자 844명(남성 340명, 여성 504명, 평균 연령 71.59세)을 대상으로 연령 증가에 따른 정적 정서 경험과 부적 정서 경험의 변화를 확인하였다. 혼합설계 분산분석(mixed ANOVA)을 통해 분석한 결과, 연구 참여 노인들은 부적 정서보다 정적 정서를 더 많이 경험하는 정서최적화 특성을 나타냈다. 노년기에 부적 정서가 급격하게 증가하고 정적 정서가 급격하게 감소할 것이라는 일반적인 믿음과 달리, 점진적인 정적 정서와 부적 정서의 감소가 나타났다. 노년기 주관적 안녕감에 정서 경험이 미치는 영향을 확인한 결과, 학력이나 경제수준과 같은 인구통계학적 특성에 비해 정서 경험이 미치는 영향이 더 컸다. 그리고, 정적 정서를 더 많이 경험하고 부적 정서를 덜 경험하는 것이 주관적 안녕감 유지에 효과적이라는 것을 확인할 수 있었다. 노년기 삶에 있어서 정서적 삶의 중요성과 노년연구에서의 종단 연구의 필요성에 대해 논의하였다.