• Title/Summary/Keyword: cer operon

Search Result 1, Processing Time 0.016 seconds

Quorum Sensing of Rhodobacter sphaeroides Negatively Regulates Cellular Poly-$\beta$-Hydroxybutyrate Content Under Aerobic Growth Conditions

  • Lee, Jeong-K.;Kho, Dhong-Hyo;Jang, Ji-Hee;Kim, Hye-Sun;Kim, Kun-Soo
    • Journal of Microbiology and Biotechnology
    • /
    • v.13 no.3
    • /
    • pp.477-481
    • /
    • 2003
  • The community escape response of Rhodobacter sphaeroides is exerted through the action of CerR and CerI, which code for a LuxR-type regulatory protein and acylhomoserine lactone synthase, respectively. Deletion of chromosomal DNA including cerR and cerI (mutant RI) or insertional interruption of cert (mutant AP3) resulted in two-fold increase in the cellular poly-${\beta}$-hydroxybutyrate (PHB) content In comparison with the wild-type under aerobic growth conditions. The PHB synthase (PhbC) activities of the cer mutants were doubled, and the enzyme expression was regulated at the level of phbC transcription. Thus, CerR, possibly in response to autoinducer (AI), appears to modulate the PHB content of aerobically grown cells by downregulating phbC transcription.