• Journal of the Korean Geographical Society
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• v.37 no.4
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• pp.337-356
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• 2002

It was proven that the spatial distribution pattern of the parking lots in Cheongju city was clarified through the concentric expansion of the parking lots, which started out from the center for commercial and business function, into the periphery regions. First of ail, in terms of the spatial distribution pattern of the parking lot according to the operational subject, the parking lot is distributed through private, public, and parking lots attached to buildings in the center, and in its adjacent regions, public and parking lots attached to buildings are distributed. And public parking lot, private and parking lots attached to buildings are distributed in sector pattern. And in terms of facility structure, more than half of the parking lots attached to buildings in Cheongju city are composed of Parking lots of self-driver type. In the case of the center, regional structure of self-Parking type of parking lots attacked to buildings, self-parking type lots on plane street parking, mechanical parking types attached to buildings, self-parking type of plane non-street diversely appear to be combined types, its spatial facility distribution patterns appear to be concentric patterns. Also, in terms of the spatial distribution pattern of the parking lots according to the contact types, with the centralizing of a center, southwestern regions show hourly and monthly charged parking lots; northeastern regions show free parking lots. The spatial distribution pattern of the parking lots as above, reflects the regional distribution change of the establishment and residential location.

• The Korean Journal of Malacology
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• v.22 no.1 s.35
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• pp.39-49
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• 2006

Ultrastructure of germ cell differentiation during supermatogenesis and the reproductive cycle in male Patinopecten yessoensis was studied by histological and cytological observations. The gonadosomatic index (GSI) in males rapidly increased and reached a maximum in April when seawater temperature gradually increased. Then the GSI gradually decreased from May through July when spawning occurred. Accordingly, monthly changes in the GSI in males coincided with testicular maturation and spawning periods. The sperm morphology of P. yessoensis belongs to the primitive type and showed general characteristics of external fertilization species. The head of the spermatozoon is approximately $3.50{\mu}m$ in length: the sperm nucleus and acrosome are approximately $2.90{\mu}m\;and\;0.60{\mu}m$ in length, respectively. The nuclear type of the spermatozoon is vase in shape, and the acrosome is cone type. The axoneme of the tail flagellum consists of nine pairs of microtubules at the periphery and a pair of central microtubules in the center The satellite body (which is formed by the centriole) and four mitochondria appear in the middle piece of the spermatozoon. The spawning period was from April through July and the main spawning occurred from May to June when seawater temperatures gradually increased. The reproductive cycle of this species can be classified into five successive stages; early active stage (September to November), late active stage (October to March), ripe stage (February to August), spawning stage (April to July), and spent/inactive stage (July to November).

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1762-1767
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• 2008

Image contrast of whole bacteria was compared in Staphylococcus aureus and Escherichia coli depending on pre-stain suspension liquids by energy-filtering transmission electron microscopy. The two bacterial strains were suspended in three most commonly used liquids for negative staining (triple distilled water [DW], phosphate-buffered saline [PBS], and nutrient broth [NB]) and directly observed without staining or stained with neutralized potassium phosphotungstate (PTA), respectively. Even though in low contrast, unstained bacteria were observed owing to their inherent electron density and cell shape in zero-loss (elastic scattering) images. After being suspended in PBS, unstained bacteria appeared to have higher contrast and more refined periphery than DW-suspended ones, and extracellular appendage structures such as fimbriae and flagella could be discerned. The unstained bacteria appeared to be invariably surrounded with electron-lucent precipitates, possibly from PBS. As far as delineation of the structures, the combination of DW or PBS suspension with subsequent staining provided the most satisfactory results, as evidenced by the high contrast of bacterial morphology and appendage structures. However, after being suspended in NB and stained with PTA, bacteria often had too high contrast or poor staining, with electron-dense aggregates around the bacteria. These results suggest that suspension with concentrated organic aliquots including broth media before PTA staining could deteriorate image contrast, and should be used only in dilute form for visualizing bacterial morphology and appendage structures. Moreover the contrast enhancement of unstained bacteria by salt granules would be advantageous in demonstrating bacterial sorption of environmental particles like heavy metals, maintaining minimal contrast for cell imaging.

• Archives of Plastic Surgery
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• v.37 no.6
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• pp.742-748
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• 2010

Purpose: The method of using ultrasound-assisted liposuction and excision of the remaining glandular tissue is the preferred method for treating gynecomastia and is currently used worldwide. Herein, this article described the role of ultrasound-assisted liposuction before a surgical excision in the treatment of gynecomastia. The cosmetic results were objectively evaluated. Methods: 11 patients (22 breasts) underwent ultrasoundassisted liposuction and suction-assisted lipectomy between April 2007 and January 2009. At the end of the liposuction, the remaining glandular tissue was removed through the incision used for liposuction. We evaluated the cosmetic results using ordinary scale methods on the basis of four categories (recurrence, symmetry, contour irregularity, and scar). Results: The volume of aspirates ranged between 50 and 200 cc per breast and the average weight of tissue removed by excision was 65g per breast. No complications were recorded. Regarding the cosmetic evaluation, the recurrence, contour irregularity, and scar were excellent, the symmetry was good, and the overall results represented all those cases were mostly excellent. Conclusion: Ultrasound-assisted liposuction has many advantages in the treatment of gynecomastia. When excising the remaining glandular tissue, bleeding is decreased by the use of a tumescent technique. The glandular tissue is easily mobilized and excised after being "morselized" with ultrasound-assisted liposuction. The glandular tissue is simply dissected via the suction surface. Compared the residual mound of glandular tissue beneath the nipple and areola to the periphery, it facilitates precise control of the excision.

• Proceedings of the Korean Society for Language and Information Conference
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• 2001.06a
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• pp.199-225
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• 2001

The main goal of this paper is to investigate and compare English, German and Korean non-head-bound-intensifiers such as English ‘x-self’, German ‘selbst’, and Korean ‘susulo, casin’. That is, this paper is mainly concerned with the semantic domain where the respective contributions of the expressions in question overlap. The phenomenon under discussion with the label “intensifiers” is regarded as universal, which provides the ground of the comparative/contrastive or semi-cross-linguistic study of this paper. Not only the semantic concept of intensification by these expressions but also the combination of grammatical features or syntactic behaviours thereof seem to have highly invariant common denominators among the wide varieties of languages, even if they come from apparently different language families. In comparing English, German and Korean intensifiers, this paper is interested in the more general features of the expressions in question rather than some language-specific idiocyncracies. Intensifiers work similarly not only in English and German, but also in Korean. Each of three languages under investigation provides some sort of a safegard against confusing instances and misleading judgements on the issues under discussion. Morphologically, however, English expressions in question agree with their rele-vant NP in number, gender and person. Whereas German and Korean counterparts do not have such specific morphological properties. Intensifiers in their non-head-bound-use are subject-oriented, just as in their head-bound use. Non-head-bound-intensifiers differ from head-bound-intensifiers mostly in their syntactic behaviours or distributional properties, whereas they share the semantic domain “intensification” regarding relevant subject-NP. They introduce an ordering and distinguish center and periphery, and ‘self-involvement (directness of involvement)’seems a additional possible characterisation of the relevant dimension of these intensifiers in common. An assertion of identity also can be reg

• The Plant Pathology Journal
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• v.29 no.1
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• pp.17-30
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• 2013

Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treat-ments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3.

• Journal of applied mathematics & informatics
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• v.41 no.1
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• pp.51-70
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• 2023

The flow of an incompressible Ellis fluid in an inclined asymmetric channel, driven by peristaltic waves was studied under low Reynolds number and long wavelength assumptions. The wave on each side of the channel are assumed to be an infinite train of sinusoidal waves, both having the same constant wave speed and wavelength however, they vary in wave amplitude, channel half width and phase angle. We derived expressions for the axial and transverse velocities, volume flow rate, pressure rise per unit wavelength and streamlines. The effects of varying the wave amplitudes, the phase angle, the channel width, the angle of inclination of the channel as well as the fluid parameters on the flow were analyzed. Trapping conditions were determined and the presence of reflux highlighted using the streamlines for the necessary channel and fluid conditions. By varying the fluid parameters, changes in the fluid that deviated from the Newtonian case resulted in a reduction in the axial velocity in the neighborhood of the center of the channel and a simultaneous increase in the velocity at the periphery of the channel. A nonlinear relation was observed with the pressure rise and the volume flow rate. This nonlinear relation is more pronounced with an increase in the absolute value of the volume flow rate. For Newtonian fluids a linear relation exists between these two variables. The fluid parameters had little effects on the streamlines. However, variations of the wave amplitudes, volume flow, channel width and phase angle had greater effects on the streamlines and hence the trapped region.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1598-1608
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• 2013

An optimum dietary carbohydrate content is important for maximum fish growth. In this study, we fed Wuchang bream (Megalobrama amblycephala) with either control diet (30.42%) or high carbohydrate diet (52.92%) for 90 d. Fish were fed to apparent satiation three times daily in an aquarium with automatic temperature control and circulated water. Growth performance, plasma biochemical parameters, hepatic morphology and enzyme activities were determined. It was shown that compared to fish fed control diet, fish fed high carbohydrate diet had higher plasma triglyceride and cortisol levels for d 90, and lower alkaline phosphatase level for d 45, lower hepatic superoxide dismutase and total antioxidative capacity for d 90, higher malondialdehyde for d 45 and glycogen content for d 45 and 90 (p<0.05). Histological and transmission electron microscopy studies showed that hepatocytes of fish fed high carbohydrate diet contained large lipid droplets, causing displacement of cellular organelles to periphery of hepatocytes. The relative level of hepatic heat shock protein 70 (HSP70) mRNA of Wuchang bream fed high carbohydrate diet was significantly higher than that of fish fed the control diet for 90 d (p<0.05). These changes led to decreased specific growth rate and increased feed conversion ratio (p<0.05). Upon hypoxia challenge, fish fed high carbohydrate diet had higher cumulative mortality than those fed the control diet (p<0.05). These results suggested that high dietary carbohydrate (52.92%) was detrimental to the growth performance and health of Wuchang bream.

• The Korean Journal of Malacology
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• v.26 no.4
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• pp.311-316
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• 2010

Spermatid differentiations during spermiogenesis and mature sperm ultrastructure in male Crassostrea nipponica were investigated by transmission electron microscope observations. The morphology of the spermatozoon of this species has a primitive type and is similar to those of other bivalves. Mature spermatozoa consist of broad, cap-shaped acrosomal vesicle and an axial rod in subacrosomal materials on an oval nucleus showing deeply invaginated anteriorly, two triplet substructure centrioles surrounded by four spherical mitochondria, and satelite fibres, which appear near the distal centriole. The acrosomal vesicle of spermatozoa of C. nipponica resemble to those of other investigated ostreids. Especially, two transverse bands (stripes) appear at the anterior region of the acrosomal vesicle, unlikely 2-3 transverse bands (stripes) in C. gigas. It is assumed that differences in this acrosomal substructure are associated with the inability of fertilization between the genus Crassostrea and other genus species in Ostreidae. Therefore, we can use sperm morphology in the resolution of taxonomic relationships within the Ostreidea. The sperm is approximately $48-50{\mu}m$ in length including an oval sperm nucleus (about $1.0{\mu}m$ in length and $1.41{\mu}m$ in width), an acrosome (about $0.48{\mu}m$ in length and 0.30 in width) and tail flagellum ($46-48{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9 + 2 structure. These morphological charateristics of acrosomal vesicle belong to the family Ostreidae in the subclass Pteriomorphia.

• The Korean Journal of Malacology
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• v.26 no.1
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• pp.33-43
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• 2010

The ultrastructure of germ cells during spermatogenesis and some characteristics of sperm morphology in male Mytilus coruscus, which was collected on the coastal waters of Gyeokpo in western Korea, were investigated by transmission electron microscope observations. The morphology of the spermatozoon has a primitive type and is similar to those of other bivalves in that it contains a short midpiece with five mitochondria surrounding the centrioles. The morphologies of the sperm nucleus type and the acrosome shape of this species have an oval and modified cone shape, respectively. In particular, the axial rod is observed between the nucleus and acrosome of the sperm. The spermatozoon is approximately $45-50{\mu}m$ in length including a sperm nucleus (about $1.46{\mu}m$ in length), an acrosome (about $3.94{\mu}m$ in length) and tail flagellum (approximately $40-45{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. Some special charateristics of sperm morphology of this species in the genus Mytilus are (1) acrosomal morphology, (2) the number of mitochondria in the midpiece of the sperm, and (3) the existence of a satellite. The axial rod appears in the acrosome and sperm nucleus as one of the characteristics seen in several species of the subclass Pteriomorphia, unlikely the subclass Heterodonta containing axial filament instead of the axial rod. The number of mitochondria in the midpiece of the sperm of this species in the family Mytilidae are five, as one of common characteristics appeared in most species in the family Mytilidae. Most of Mytilus species contain a satellite body which is attached to the proximal centriole in the middle piece of the sperm, as one of common characteristics of sperm morphology in the family Mytilidae.