• Clinical and Experimental Pediatrics
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• v.61 no.10
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• pp.307-314
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• 2018

The incidence of type 1 diabetes mellitus (T1DM) in children and adolescents is increasing worldwide. Combined effects of genetic and environmental factors cause T1DM, which make it difficult to predict whether an individual will inherit the disease. Due to the level of self-care necessary in T1DM maintenance, it is crucial for pediatric settings to support achieving optimal glucose control, especially when adolescents are beginning to take more responsibility for their own health. Innovative insulin delivery systems, such as continuous subcutaneous insulin infusion (CSII), and noninvasive glucose monitoring systems, such as continuous glucose monitoring (CGM), allow patients with T1DM to achieve a normal and flexible lifestyle. However, there are still challenges in achieving optimal glucose control despite advanced technology in T1DM administration. In this article, disease prediction and current management of T1DM are reviewed with special emphasis on biomarkers of pancreatic ${\beta}-cell$ stress, CSII, glucose monitoring, and several other adjunctive therapies.

• Proceedings of the IEEK Conference
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• 2009.05a
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• pp.190-192
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• 2009

Development of safety monitoring system for workers in the ship building industry is currently under progress using RFID(Radio Frequency IDentification) for successful development of the U-BUSS(Ubiquitous- Safety User Safety System). For decades, RFID technology has become a key technology to provide the real-time location system of worker and is variously used for safety monitoring system to increase productivity, improve the blasting quality and enhance the safety of working condition in the ship building industry In this paper, 2.45GHz band RTLS(Real Time Location System) technologies and the ubiquitous safety monitoring system of the ship yard's blasting cell are described.

• Journal of the Korean Society for Precision Engineering
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• v.12 no.12
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• pp.19-29
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• 1995

The basic technology for a production system represented by design, machining, assembly, and inspection, is machining technology such as CNC machine tools. etc. Direct Numerical Control, that effeciently manages NC programs is developing into Distributed Numerical Control that increases the utilization of the machining cell. It has the ability of monitoring and control, in real time, for CNC and periperial equipment. In this study, we develop a Distributed Numerical Control system that has real time and multitasking operation capability for the machining cell with various CNC's. With the consideration of economy, generalization and extension, the system is interfaced with CNC machine tools and periperial device using RS-485 network and RS-232C communication methods.

• Proceedings of the KIEE Conference
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• 2008.10c
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• pp.167-169
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• 2008

A 1.2KW Proton Exchange Membrane Fuel Cell(PEMFC) stack(Nexa Power Module) is installed to motor driven general scooter. The 24V, 650W BLDC motor is used for actuator and 2nd battery(24V, 4Ah) is used for stable starting of this motor. The operating information of Nexa Power Nodule is transmitted by RS-232 serial communication to notebook PC, and that data is analysed and displayed by the LabVIEW monitoring system.

• 한국신재생에너지학회:학술대회논문집
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• 2009.11a
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• pp.209-212
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• 2009

수소에너지의 무궁한 가능성 때문에 관련 기술은 미래 국가 경쟁력을 좌우할 것이며, 이러한 수소에너지의 핵심으로 수소제조공정 및 수소스테이션 관련 기술은 국가연료전지 시장을 비롯한 수소연료전지자동차 산업 전반에 커다란 영향을 미칠 것으로 예상되고 있다. 그러므로 세계 각국은 수소에너지를 미래에너지원으로 개발하기 위하여 대규모로 기술개발 및 실증연구를 실시하고 있으며, 주로 수소연료전지자동차 개발과 아울러 수소스테이션 개발에 대한 인프라 구축에 대해 이루어지고 있다. 국내에서도 국가주도로 수소스테이션 건설이 이루어지고 있으며, 본 연구에서는 수소연료전지자동차를 이용하여 수소인프라와의 모니터링에 대한 현황에 대해 논의하고자 한다.

• Proceedings of the KIPE Conference
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• 2010.11a
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• pp.359-360
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• 2010

In smart grid system which uses photovoltaic system, fuel cells and so on, a program implemented with C language is used for control and measurement. When using programs implemented with C language GUI is difficult to watch control and monitor the smart grid system. But LabVIEW is a graphical programming language and it is easy to design GUI screen and to manage many variables such as real-time output of electric power including solar cell, wind power system and fuel cell. This paper suggests LabVIEW and C-language TCP/IP communication for smart grid monitoring system i mplemented with C-language.

• Journal of the Korean Society for Nondestructive Testing
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• v.29 no.4
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• pp.299-307
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• 2009

Emerging fiber optic sensor technologies have shown great potential to overcome the difficulties associated with conventional sensors. Fiber optic sensors are immune to EM noise and electric shock and thus can be used in explosion-prone areas. Several kinds of fiber optic sensors have been developed over the last two decades to take advantage of these merits. There have also been many field applications of fiber optic sensors for structural health monitoring as NDT/HDE. However, very few sensors, particularly a load cell have been successfully commercialized. This Paper Presents a load cell using fiber Bra99 gra1ing (FBG) sensors. The shape of the load cell is a link type, and three FBG sensors are used for measuring strains at three different points. Especially, these strains are processed with a differential method in order to exclude common mode noise such as temperature. Moreover, the sensitivity, the linearity and the resolution of the load cell were successfully verified from the experiment of tension test.

• Proceedings of the Microbiological Society of Korea Conference
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• 2008.05a
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• pp.171-171
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• 2008

We monitored the occurrence of human enteric viruses in urban rivers by cell culture-PCR and RT-nested PCR. Water samples were collected monthly or semimonthly between May 2002 and March 2003 in four urban tributaries. Enteric viruses were detected by RT-nested PCR and cell culture-PCR based on a combination of Buffalo Green monkey kidney (BGMK) and A549 cell lines, followed by phylogenetic analysis of amplicons. By RT-nested PCR analysis, 45 (77.6%), 32 (55.2%), 32 (55.2%), 26 (44.8%), 12 (20.7%), 2 (3.4%), 4 (6.9%), and 4 (6.9%) of 58 samples showed positive results with adenoviruses, enteroviruses, noroviruses (NV) genogroup I (GI) and II (GII), reoviruses, hepatitis A viruses, rotaviruses and sapoviruses, respectively. Adenoviruses were most often detected and only eight (13.8%) samples were negative for adenoviruses and positive for other enteric viruses in the studied sites. Thirty-one (77.5%) of the 40 samples were positive for infectious adenoviruses and/or enteroviruses based on cell culture-PCR, and the frequency of positive samples grown on A549 and BGMK (65.0%) was higher than that grown on BGMK alone (47.5%). The occurrence of each enteric virus, except reoviruses and hepatitis A viruses was not statistically correlated with the water temperature and levels of fecal coliforms according to Binary logistic regression model. By sequence analysis, most strains of adenoviruses and enteroviruses detected in this study are similar to the causative agent of viral diseases in Korea and most NV GI- and GII-grouped strains were closely related to the reference strains from China and Japan, and GII/4-related strains had similar sequences to strains recognized as a worldwide epidemic outbreak. Our results suggested that monitoring human enteric viruses is necessary to improve microbial quality and cell culture-PCR using the combination of A549 and BGMK cells and the adenovirus detection by PCR could be useful for monitoring viral contamination in the aquatic environment.

• Molecules and Cells
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• v.42 no.11
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• pp.783-793
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• 2019

When endoplasmic reticulum (ER) functions are perturbed, the ER induces several signaling pathways called unfolded protein response to reestablish ER homeostasis through three ER transmembrane proteins: inositol-requiring enzyme 1 (IRE1), PKR-like ER kinase (PERK), and activating transcription factor 6 (ATF6). Although it is important to measure the activity of ATF6 that can indicate the status of the ER, no specific cell-based reporter assay is currently available. Here, we report a new cell-based method for monitoring ER stress based on the cleavage of $ATF6{\alpha}$ by sequential actions of proteases at the Golgi apparatus during ER stress. A new expressing vector was constructed by using fusion gene of GAL4 DNA binding domain (GAL4DBD) and activation domain derived from herpes simplex virus VP16 protein (VP16AD) followed by a human $ATF6{\alpha}$ N-terminal deletion variant. During ER stress, the GAL4DBD-VP16AD(GV)-$hATF6{\alpha}$ deletion variant was cleaved to liberate active transcription activator encompassing GV-$hATF6{\alpha}$ fragment which could translocate into the nucleus. The translocated GV-$hATF6{\alpha}$ fragment strongly induced the expression of firefly luciferase in HeLa Luciferase Reporter cell line containing a stably integrated 5X GAL4 site-luciferase gene. The established double stable reporter cell line HLR-GV-$hATF6{\alpha}$(333) represents an innovative tool to investigate regulated intramembrane proteolysis of $ATF6{\alpha}$. It can substitute active pATF6(N) binding motif-based reporter cell lines.

• International Journal of Stem Cells
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• v.15 no.1
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• pp.95-103
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• 2022

Background and Objectives: In recent years, brain organoid technologies have been the most innovative advance in neural differentiation research. In line with this, we optimized a method to establish cerebral organoids from feeder-free cultured human pluripotent stem cells. In this study, we focused on the consistent and robust production of cerebral organoids comprising neural progenitor cells and neurons. We propose an optimal protocol for cerebral organoid generation that is applicable to both human embryonic stem cells and human induced pluripotent stem cells. Methods and Results: We investigated formation of neuroepithelium, neural tube, and neural folding by observing the morphology of embryoid bodies at each stage during the cerebral organoid differentiation process. Furthermore, we characterized the cerebral organoids via immunocytochemical staining of sectioned organoid samples, which were prepared using a Cryostat and Vibratome. Finally, we established a routine method to generate early cerebral organoids comprising a cortical layer and a neural progenitor zone. Conclusions: We developed an optimized methodology for the generation of cerebral organoids using hESCs and hiPSCs. Using this protocol, consistent and efficient cerebral organoids could be obtained from hiPSCs as well as hESCs. Further, the morphology of brain organoids could be analyzed through 2D monitoring via immunostaining and tissue sectioning, or through 3D monitoring by whole tissue staining after clarification.