• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.25 no.3
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• pp.366-379
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• 2015

Objectives: The objective of this study is to assess airborne particulate matter(PM) pollution and its effect on health of residents living near Ansim Briquette Fuel Complex in Daegu metropolitan region. Methods: The California Puff(CALPUFF) dispersion model, version 5.8, which can estimate the dispersion direction and range of airborn $PM_{10}$ was used to determine the possible areas affected by $PM_{10}$ pollutants emitted from Ansim briquette fuel complex. The CALPUFF modeling with 200 m grid-cell resolution was performed based on $PM_{10}$ emissions estimated from the amount of coal consumption in the fuel complex for four months in 2012. The Weather Research and Forecasting(WRF) fields were processed using CALMET to produce CALPUFF-ready meteorological inputs. Also, the distance from Ansim Briquette Fuel Complex to the residence of each environmental pneumoconiosis patient was analyzed. In addition, the affecting region of the pollutants emitted from briquette factories in Ansim Briquette Fuel Complex was determined. Results: CALPUFF modeling results showed that the highest concentrations of $PM_{10}$ were found near around the fuel complex. The modeled $PM_{10}$ distributions were characterized by significant decreases in concentration with distance from the complex. Seasonally, the highest concentration of $45{\mu}g/m^3$ was calculated in October which was mostly due to the distinct variation of amount of emission. Additional modeling with the maximum $PM_{10}$ emission of about 88 tons per year in 1986 showed that the highest concentration in October was nearly increased by 8 times than the concentration modeled with emission of 2010. As a result of medical examination and interviews for the residents in Ansim Briquette Fuel Complex and its surroundings, 8 environmental pneumoconiosis patients were found. These patients do not have occupational exposure and history. These patients have lived 0.3~1.1 km area in Ansim Briquette Fuel Complex and its surroundings. Conclusions: Airborne particles emitted from Ansim Briquette Fuel Complex can contribute to significant increase in $PM_{10}$ concentration in residential areas near around the complex. Especially, the residents near fuel complex may exposed to the pollutants emitted from the factories in Ansim Briquette Fuel Complex.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.173-183
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• 2004

This study was carried out to examine the effects of epidermal growth factor (EGF) and the number of cumulus-oocyte complexes (COCs) on in vitro maturation (IVM) of porcine immature oocytes, and on subsequent in vitro fertilization (IVF) and development (IVD). COCs were collected from antral follicles of porcine ovaries collected from abattoir, and were maturated in modified NCSU-23 (mNCSU-23) with 10% pFF, 0.6 mM cysteine, 50 ${\mu}mM{\beta}-mercaptoethanol$, 1 mM dbcAMP, 10 IU/mL PMSG and 10 IU/mL hCG, which was supplemented with or without 10 ng/mL EGF and into which 50 or 15 COCs per droplet was put. Oocytes matured in vitro, were fertilized in vitro in modified Tris-buffered medium (mTBM) with the final motile sperm concentration of 1${\times}$105 sperm/mL, and subsequently putative embryos were developed in vitro in NCSU- 23. The results are as follows. 1.In the result of IVM, 10 ng/mL EGF supplement duplicated the percentage of C4 group of COCs(41% vs 81%). But the rate of germinal vesicle breakdown (GVBD) and of nuclear maturation were not significantly different between control and EGF supplemented, or between the number of COCs per culture droplet, and there was not a significant interaction between the two factors, either. 2. In the result of IVF, there was not significantly different between control and EGF supplemented, or between the number of COCs per culture droplet, or was not a significant interaction between the two factors, in the rate of sperm penetration, in the percentage of oocytes with male pronucleus (MPN), and in the rate of polyspermy. 3. In the result of IVD, there was not significantly different between control and EGF supplemented, or between the number of COCs per culture droplet in the percentage of cleaved oocytes. There was not significantly different between the number of COCs per culture droplet, but between control and EGF supplemented (p<0.01) in the percentage of blastocysts, the number of inner cell mass (ICM), trophectoderm (TC) and total cells. There was no significant interaction between the two factors anywhere. These results suggested that 10 ng/mL EGF supplement into mNCSU-23 for IVM was effective in the production of more as well as better blastocysts during IVD through increasing the number of cells in those.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.685-691
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• 2005

Growth and DNA synthesis inhibitory effects of doenjang methanol extract and its solvent fractions on AGS human gastric adenocarcinoma cells, Hep 3B human hepatocellular carcinoma cells, HT-29 human colon cancer cells and MG-63 human osteosarcoma cells were studied. The treatment of doenjang methanol extract ($ 200{\mu}g/ml $) with the AGS, Hep 3B, HT-29 and MG-63 cancer cells after 6 days of incubation inhibited the growth of cancer cells by $32\%$, $51\%$, $84\%$ and $33\%$, respectively. To separate active compounds of doenjang, doenjang methanol extract was fractionated with dichloromethane, ethylacetate, and buthanol. Among the solvent fractions, the dichloromethane and ethylacetate fractions showed the highest growth inhibitory effects on various cancer cells. For example, the dichloromethane and ethylacetate fractions ($200a{\mu}g/ml$) sig-nificantly inhibited the growth of various cancer cells by $89\∼96\%$ and$62\∼86\%$, respectively. DNA synthesis of AGS and Hep 3B cancer cells was significantly inhibited by adding dichloromethane fraction ($200{\mu}g/ml$) up to $94\%$ and $80\%$, respectively. Similarly, the ethylacetate fraction ($ 200\mug/ml $) showed a $ 95\% $ inhibition rate of DNA synthesis in AGS cells. These results suggest that the dichloromethane and ethylacetate fractions have specific active compounds, which will explain this anticancer effect of doenjang.

• Journal of Advanced Marine Engineering and Technology
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• v.40 no.1
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• pp.28-33
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• 2016

Batteries are used for main power engine in the fields such as mobiles, electric vehicles and unmanned submarines, for starter and lamp driver in general automotive, for emergency electric source in ship. These days, lead-acid and the lithium ion batteries are increasingly used in the fields of the secondary battery, and the lead-acid battery has a low price and safety comparatively, The lithium ion battery has a high energy density, excellent output characteristics and long life, whereas it has the risk of explosion by reacting with moisture in the air. But Recently, due to the development of waterproof, fireproof, dustproof technology, lithium batteries are widely used, particularly, because their usages are getting wider enough to be used as a power source for hybrid ship and electric propulsion ship, it is necessary to manage more strictly. Hybrid ship has power supply units connected to the packets to produce more than 500kWh large power source, and therefore, A number of the communication modules and wires need to implement the wire inspection and monitor system(WIIMS) that allows monitoring server to transmit detecting voltage, current and temperature data, which is required for the management of the batteries. This paper implements a low price type wireless inspection and monitoring system(WILIMS) of the lithium ion battery for hybrid vessels using BLE wireless communication modules and power line modem( PLM), which have the advantages of low price, no electric lines compared to serial communication inspection systems(SCIS). There are state of charge(SOC), state of health(SOH) in inspection parts of batteries, and proposed system will be able to prevent safety accidents because it allows us to predict life time and make a preventive maintenance by checking them at regular intervals.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.186-195
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• 2015

This research was conducted to investigate effect the plant growth regulators (PGRs) on the characteristics of fruit and flesh softening, using GA3 and thidiazuron (TDZ) treatments in 'Heukboseok' grapes. The total yield obtained under PGR treatment was 88.3% lower than the target production when a single treatment with $GA_3$ of low concentrations was used, but the expected yield was recovered by combined treatment with TDZ and $GA_3$. When harvested on the basis of color, the harvest rate up to 100 days after full bloom (DAFB) was low with the $GA_3$ single treatment, but was increased by the addition of TDZ, with the second TDZ mixed treatment being particularly effective. The soluble solids content in the PGR-treated samples demonstrated no significant changes after 90 DAFB, while the acidity content decreased rapidly starting from 90 DAFB. Measured on the basis of sugar and acidity content, maturity was reached much earlier in treated fruit than in the non-treated fruit. Firmness was maintained at a higher level until the final harvest time after PGR treatment compared to untreated grapes according to epidermis thickness and flesh density increase to activity cell division and expansion by $GA_3$ and TDZ. In particular, the fruit quality was improved based on the delay of softening in primary and secondary treatments of $25mg{\cdot}L^{-1}\;GA_3+2.5mg{\cdot}L^{-1}$ TDZ. The production of seedless fruit was difficult, even with the inclusion of TDZ, reaching the highest seedless rate of only 65.5%. Fruit cracking was rare, occurring at a rate of about 0.0~0.9% in all treatments. Accordingly, 'Heukboseok' grapes should be harvested within 100 days after full bloom (DBFB) before a rapid decrease of firmness, conferred by primary and secondary treatments with $25mg{\cdot}L^{-1}\;GA_3+2.5mg{\cdot}L^{-1}$ TDZ.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.727-735
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• 2016

This research was conducted to investigate the influence of various pre-planting $NO_3:NH_4$ ratios in the coir dust+peatmoss+perlite (3.5:3.5:3, v/v/v) medium on the growth of tomato plug seedlings (cv. Dotaerang Dia), changes in chemical properties of root media, and tissue nutrient contents. With the fixation of N concentration to $300mg{\cdot}L^{-1}$, the $NO_3:NH_4$ ratios were adjusted to 100:0, 73:27, 50:50, 27:73, 0:100. Then, root media containing various $NO_3:NH_4$ ratios and equal concentration of other essential nutrients were filled into 50-cell plug trays and the seeds of 'Dotaerang Die' tomato were sown. The investigation of seedling growth and tissue analysis for mineral nutrient contents based on the dry weight of above-ground tissue were conducted 6 weeks after sowing. As seedlings grew, the EC decreased quickly and pH increased gradually in the all treatment media. The plant height, fresh weight and dry weight of seedlings in the treatment of 50:50 ($NO_3:NH_4$) were 29.0 cm, 13.7 g, and 1.21 g, respectively, which were the highest among treatments tested. However, the seedlings in the treatment of 0:100 ($NO_3:NH_4$) had 26.5 cm, 11.2 g, and 0.92 g in plant height, fresh weight and dry weight, respectively. These were the poorest among the treatments tested. The tissue contents of N were 2.77 to 3.22% in all the treatments. The treatment of 27:73 ($NO_3:NH_4$) had the highest contents of Fe, Mn and Zn and that of 0:100 ($NO_3:NH_4$) had the lowest contents of Mg, Na, Cu, Mn and Zn among the treatments tested. The results indicate that $NH_4$ ratio should be lower than 50% in the coir dust+peatmoss+perlite (3.5:3.5:3, v/v/v) medium for seedling growth of tomato and the optimum ratio will be used to draw up guide lines for plug seedling production.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.616-625
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• 2016

This research was conducted to investigate the optimum level of nitrogen incorporated during formulation of root media as pre-plant fertilizer on the growth of plug seedlings of 'Bool-am No.3' Chinese cabbage. A root medium was formulated by blending peatmoss:coir dust:perlite at a ratio of 3.5:3.5:3.0 (v/v/v). The nitrogen was incorporated in the seven treatments at a rate of 0, 100, 250, 500, 750, 1,000, and $1,500mg{\cdot}L^{-1}$ during the root medium formulation. The concentrations of other essential nutrients except nitrogen were controlled to equal in all treatments. Then, the root medium was packed into 72-cell plug trays and seeds were sown. The growth measurements as well as tissue and soil solution analysis for nutrients were conducted 2 and 4 weeks after seed sowing. As seedlings grew, the pH in the extracted solution of all treatments tended to decrease. The decreases in the treatments of high N concentrations were more severe than those with low N, but the differences among treatments were not statistically significant. The differences of EC in extracted solution of root media among treatments were sizable until week 3, but the differences began to lessen and the EC decreased in all treatments after week 4. Growth of the aerial parts of plug seedlings at 2 weeks after sowing were highest in the $100mg{\cdot}L^{-1}$ and lowest in the $1,500mg{\cdot}L^{-1}$ treatments, but those at 4 weeks after sowing were highest in the $500mg{\cdot}L^{-1}$ and lowest in the $0mg{\cdot}L^{-1}$ treatments among all treatments tested. The tissue N content was highest and lowest in the treatments of 250 and $1,000mg{\cdot}L^{-1}$, respectively, when tissues were harvested at 4 weeks after sowing and analysed based on the dry weight of above-ground tissue. The contents of micronutrients were the highest in the 1,000 and $1,500mg{\cdot}L^{-1}$ treatments among all treatments. The results shown above indicate that the $250mg{\cdot}L^{-1}$ of pre-plant N and elevation of post-plant N concentration to above $100mg{\cdot}L^{-1}$ are suitable for raising plug seedlings of Chinese cabbage using inert media.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.736-745
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• 2016

This research was conducted to evaluate the impact of various pre-planting $NH_4{^+}:NO_3{^-}$ ratios on the growth of plug seedlings of 'Bool-am No.3' Chinese cabbage. With fixation of the pre-planting N concentrations to $300mg{\cdot}kg^{-1}$ in a peatmoss+coir dust+perlite (3.5:3.5:3, v/v/v) medium, the $NH_4{^+}:NO_3{^-}$ ratios were varied to 0:100, 27:73, 50:50, 73:27, 100:0. Then, the each of root media containing various ratios of $NH_4{^+}:NO_3{^-}$ as well as equal concentrations of other essential nutrients was packed into 72-cell plug trays. After seeds of 'Bool-am No.3' Chinese cabbage were sown, the seedling growths were measured 2 and 4 weeks after sowing. The weekly analysis of root media and end-crop tissue analysis for mineral nutrients 4 weeks after seed sowing were also conducted. As the seedlings grew up, the pH of the root media increased, however ECs in all treatments of $NH_4{^+}:NO_3{^-}$ ratios decreased. The concentrations of K, Ca and Mg in root media were higher in the treatments of $NH_4{^+}:NO_3{^-}$ (100:0) and $NH_4{^+}:NO_3{^-}$ (73:27) than those of $NH_4{^+}:NO_3{^-}$ (0:100) and $NH_4{^+}:NO_3{^-}$ (27:73) 2 weeks after seed sowing. But the concentrations of K, Ca, Mg and Zn were get lowered in all treatments and the differences among treatments were not significant 4 weeks after sowing. The highest $NH_4{^+}$ and lowest $NO_3{^-}$ concentrations of the root media were observed in the $NH_4{^+}:NO_3{^-}$ (100:0) among all treatments. Contrary to these, the treatment of $NH_4{^+}:NO_3{^-}$ (0:100) had the lowest $NH_4{^+}$ and highest $NO_3{^-}$ concentrations. The seedling growth in terms of fresh and dry weights of aerial part were the highest in the treatment of $NH_4{^+}:NO_3{^-}$ (23:73) at 2 weeks after sowing and those of $NH_4{^+}:NO_3{^-}$ (50:50) at 4 weeks after sowing. The survival rate of seedlings in $NH_4{^+}:NO_3{^-}$ (100:0) treatment were 19% and the growth of aerial part 4 weeks after sowing was the poorest among all treatments tested. The results mentioned above indicate that the pre-planting $NH_4{^+}$ ratio in inert media should not exceed 25% in plug stage 1 through 3 (until 2 true leaf development) and 50% in plug stage 4 (after 2 true leaves to transplant).

• Journal of Life Science
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• v.27 no.8
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• pp.909-918
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• 2017

Ice plant (Mesembryanthemum crystallinum L.) was fermented in brine in the form of mulkimchi (IPMB), and its contents of organic acid and cyclitols and biological activities were compared with those before fermentation. The pH of the IPMB continuously decreased until the sixth day of fermentation. The lactic acid yield was greatest on the fourth day. D-pinitol in ice plant mulkimchi solids (IPMS) decreased during fermentation. However, myo-inositol and D-chiro-inositol increased. The radical scavenging activities of ABTS and DPPH, in addition to the activity of FRAP, of the IPMS extract were generally higher after fermentation, with the activities highest on the fifth ($79.09{\pm}0.69%$), fourth ($87.55{\pm}1.21%$), and sixth ($78.72{\pm}0.99%$) days of fermentation, respectively, when treated with 1 mg/ml of the extract. As shown by a lipid/MA assay, antioxidant activity was generally higher after fermentation. The viability of BNL CL.2 cells damaged by t-BHP, $H_2O_2$, and ethanol was $14.19{\pm}0.98$, $13.80{\pm}2.25$, and $25.89{\pm}2.90%$, respectively. When treated with $200{\mu}g/ml$ of IPMS extract, the cell viability was $57.06{\pm}4.52%$ on the first day, and $66.06{\pm}1.36%$ on the fourth day, and $50.07{\pm}04.85%$ on the sixth day of fermentation. Hepatocyte protective effects did not increase significantly after fermentation. ${\alpha}-glucosidase$ inhibitory activity was quite high, with a range of $83.52{\pm}2.69$ to $92.79{\pm}2.16%$, and the activity increased gradually in all the groups over the fermentation period. There was no clear correlation between ${\alpha}-amylase$ inhibitory activity and fermentation.

• Proceedings of the KSAR Conference
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• 2000.10a
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• pp.10-12
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• 2000

Members of the glycoprotein family, which includes CG, LH, FSH and TSH, comprise two noncovalently linked $\alpha$- and $\beta$-subunits. Equine chorionic gonadotropin (eCG), known as PMSG, has a number of interesting and unique characteristics since it appears to be a single molecule that possesses both LH- and FSH-like activities in other species than the horse. This dual activity of eCG in heterologous species is of fundamental interest to the study of the structure-function relationships of gonadotropins and their receptors. CG and LH $\beta$ genes are different in primates. In horse, however, a single gene encodes both eCG and eLH $\beta$-subunits. The subunit mRNA levels seem to be independently regulated and their imbalance may account for differences in the quantities of $\alpha$ - and $\beta$ -subunits in the placenta and pituitary. The dual activities of eCG could be separated by removal of the N-linked oligosaccharide on the $\alpha$-subunit Asn 56 or CTP-associated O-linked oligosaccharides. The tethered-eCG was. efficiently secreted and showed similar LH-like activity to the dimeric eCG. Interestingly, the FSH-like activity of the tethered-eCG was increased markedly in comparison with the native and wild type eCG. These results also suggest that this molecular can implay particular models of FSH-like activity not LH-like activity in the eCG/indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion. A single-chain analog can also be constructed to include additional hormone-specific bioactive generating potentially efficacious compounds that have only FSH-like activity. The LH/CG receptor (LH/CGR), a membrane glycoprotein that is present on testicular Leydig cells and ovarian theca, granulosa, luteal, and interstitial cells, plays a pivotal role in the regulation of gonadal development and function in males as well as in nonpregnant and pregnant females. The LH/CGR is a member of the family of G protein-coupled receptors and its structure is predicted to consist of a large extracellular domain connected to a bundle of seven membrane-spanning a-helices. The LH/CGR phosphorylation can be induced with a phorbol ester, but not with a calcium ionophore. The truncated form of LHR also was down-regulated normally in response to hCG stimulation. In contrast, the cell lines expressing LHR-t63I or LHR-628, the two phosphorylation-negative receptor mutant, showed a delay in the early phase of hCG-induced desensitization, a complete loss of PMA-induced desensitization, and an increase in the rate of hCG-induced receptor down-regulation. These results clearly show that residues 632-653 in the C-terminal tail of the LHR are involved in PMA-induced desensitization, hCG-induced desensitization, and hCG-induced down-regulation. Recently, constitutively activating mutations of the receptor have been identified that are associated with familial male-precocious puberty. Cells expressing LHR-D556Y bind hCG with normal affinity, exhibit a 25-fold increase in basal cAMP and respond to hCG with a normal increase in cAMP accumulation. This mutation enhances the internalization of the free and agonist-occupied receptors ~2- and ~17-fold, respectively. We conclude that the state of activation of the LHR can modulate its basal and/or agonist-stimulated internalization. Since the internalization of hCG is involved in the termination of hCG actions, we suggest that the lack of responsiveness detected in cells expressing LHR-L435R is due to the fast rate of internalization of the bound hCG. This statement is supported by the finding that hCG responsiveness is restored when the cells are lysed and signal transduction is measured in a subcellular fraction (membranes) that cannot internalize the bound hormone.