• 생명과학회지
• /
• 제6권4호
• /
• pp.286-292
• /
• 1996

Angiogenesis is a fundamental process by which new blood vessels are formed. It is essential in embryo development, and wound healing. Furthermore, malignant tumor growth and metastasis are also angiogenesis-dependent. In the catilage tissue, normal angiogenesis process is suppressed. In fact, it was reported that angiogenesis-inhibitory substances were isolated from the extracts of cow and shark catilage tissue. In order to isolate genes involved in the regulation of angiogenesis from a catilage fish, we constructed a shark cDNA library from Scylliohinus torazame. We then screened the library using hyman tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) gene as a probe. Among the 4 X 10$^{4}$ plaques screened, we isolated 2 positive clones (T-1, T-2). Restriction enzyme analysis revealed that the T-1 clone contains 0.8 kb cDNA insert, and the T-2 clone contains 1.2 kb and 2.2 kb inserts, respectively. Further DNA sequence analysis shows that the DNA sequence of the T-1 clone is 53% homologous to that of the human TIMP-1 gene.

• Tribology and Lubricants
• /
• 제13권1호
• /
• pp.82-87
• /
• 1997

The modern orthopaedics frequently uses the total hip replacement in the artificial hip joint. The wear in this joint requires a re-replacement of hip joints because it is under the severe load and friction conditions. To solve these problems the previous studies have been mainly focussed on the development of new materials. The research of new materials, however, needs much time and effort since it should be experimented for its bio-compatibility, friction, and wear characteristics. To reduce the work, in this study, the finite element analysis is applied to find new combinations of bio-materials in the total hip replacement which has the excellent contact characteristics. A non-linear FEM program MARC with 5-node axisymmetric element was used for analyzing the contact stresses between the hip joints. The computed results show that in case of acetabulum UHMWP has good characteristics, in femoral head, $Al_2O_3$, and in stem, Ti6Al4V.

• 한국윤활학회:학술대회논문집
• /
• 한국윤활학회 1996년도 제24회 추계학술대회
• /
• pp.189-194
• /
• 1996

The modern orthopaedics frequently uses the total hip replacement in the artificial hip joint. The wear in this joint requires the re-replacement of the hip joint beacuse it is under the severe load and friction conditions. To solve these problems the previous studies have been mainly focused on the development of the new materials. The research of new material, however, needs much time and effort since it should be experimented for its bio-compatibility, friction, and wear characteristics. To reduce the work, in this study, the finite element analysis is applied to find the new combination of the materials in the total hip replacement which has the excellent contact characteristics. The finite element uses MARC and the 5-node aximetric element. The results show that in case of acetabulum UHMWP has good characteristics, in femoral head, the $Al_2O_3$, and in stem, Ti6Al4V.

• Archives of Plastic Surgery
• /
• 제32권1호
• /
• pp.1-4
• /
• 2005

Previous sucessful results of neocartilage formation using tissue engineering technique in immunocompromised nude mouse xenograft model were reported. For clinical application, autogenous cell is preferrable to allogenic or xenogenic cell for circumvention of immune rejection. This study evaluates the feasibility of producing a engineered cartilage using autogenous chondrocytes. Chondrocytes were isolated from the auricular catilage of New Zealand White rabbit and seeded onto PGA polymer coated with polylactic acid in round pattern(diameter 0.7 cm, thickness 0.1 cm) at a concentration $7{\times}10^7$ chondrocytes per $cm^3$. Each Autogenous Cell-polymer constructs were implanted subcutaneously into the left side of dorsum of twelve Rabbits. Polymer templates not containg cells were implanted into the right side as a control. Fifteen rabbits were sacrificed at the following intervals: 5 rabbits at nine weeks, 7 rabbits at twelve weeksNew autogenous cartilage formation which retained the approximate dimensions of origianl round polymer template in 11 of 12 cell seeded implants. Histological examination using hematoxyline and eosin stain revealed vast majority of implants developed into mature cartilage. This study opens up the possibility of autologus cell transplant to construct autogenous cartilge.

• 폴리머
• /
• 제38권3호
• /
• pp.278-285
• /
• 2014

알지네이트와 탈미네랄골분(DBP) 하이브리드 스펀지는 각각 DBP가 0, 10, 20, 40 및 80% 첨가된 스펀지로, 연골 재건을 위해 제작되었다. 세포의 증식은 MTT를 통해 관찰했으며, 형태학적, 조직학적, 생물학적 관찰 그리고 RT-PCR을 1, 2, 및 3 주에 실시하였다. 세포생존율에 있어서 20% DBP/알지네이트 스펀지에서 다른 스펀지보다도 가장 좋은 결과를 보였으며, 광학전자현미경 관찰에서 또한 20% 스펀지에서 가장 좋은 세포부착률과 증식률을 확인하였다. 마지막으로, 조직학적 평가에서 20% 스펀지에서 연골세포의 표현형이 가장 잘 유지됨과 동시에 산성뮤코 다당류와 제2형 콜라겐이 잘 형성된 것을 확인하였다. 이로써 DBP/알지네이트 하이브리드 스펀지는 연골 조직공학을 위한 지지체로써 활용될 수 있을 것이다.