• The Journal of Korean Society of Virology
• /
• v.30 no.1
• /
• pp.39-49
• /
• 2000

The genome of Hantaan virus, the prototype of the hantavirus genus, is composed of three segmented, single stranded negative sense RNA genome. The 5' and 3' termini of the Hantaan virus RNA genome contain noncoding regions (NCRs) that are highly conserved and complementary to form panhandle structures. There are some reports that these NCRs seems to control gene expression and viral replication in influenza virus and vesicular stomatitis virus. In this study, we examined whether NCRs in Hantaan virus playa role in expression of the viral nucleocapsid protein (Np) and foreign (luciferase) gene. The 5' and/or 3' NCR-deleted mutants were constructed and analysed. The Np expression of 5' NCR-deleted clone was similar to that of the clone containing full S genome. In the case of 3' NCR-deleted clone, it showed 40% reduction. To investigate the role of NCR in foreign gene expression, the clones which are replaced ORF of Hantaan viral Np gene with that of luciferase gene were constructed. The results were similar to those of the experiments using Np gene. These results suggest that 3' NCR is more important than 5' NCR in protein expression. To find out a critical region of 3' NCR in protein expression, several clones with a deleted part of 3' NCR were constructed and analyzed. The deletion of the conserved region in 3' NCR showed $20{\sim}30%$ decrease in Np expression. However there were no change in luciferase activities between clones with or without non-conserved region of 3' NCR. These results suggest that the 3' NCR of Hantaan virus S genome, especially conserved region in 3' NCR, plays an important role in the expression of Hantaan viral Np and foreign genes.

• Journal of Genetic Medicine
• /
• v.4 no.1
• /
• pp.80-83
• /
• 2007

A 36-year-old pregnant woman was referred for amniocentesis at 19.5 weeks gestation because of advanced maternal age and evidence of increased risk for Edward syndrome in the maternal serum screening test. Cytogenetic analysis of the cultured amniotic fluid cells revealed mosaicism for ring chromosome 11: 46,XX,r(11)[65]/ 45,XX,-11[16]/ 46,XX [34]. Parental karyotypes were normal. A targeted ultrasound showed intrauterine grow th restriction (IUGR). Cordocentesis was performed to characterize the ring chromosome and to rule out tissue specific mosaicism. Karyotype was confirmed as 46,XX,r(11) (p15.5q24.2)[229]/45,XX,-11[15]. And a few new form of ring w ere detected in this culture. The deletion of subtelomeric regions in the ring chromosome were detected by fluorescent in situ hybridization (FISH). The pregnancy was terminated. The fetal autopsy showed a growth-retarded female fetus with rocker bottom feet. We report a case of prenatally detected a de novo ring chromosome 11.

• Journal of Korean Philosophical Society
• /
• v.130
• /
• pp.381-414
• /
• 2014

This paper aims to elaborate on the foundation of the colonialism, which comes from Natural Laws by John Locke and the extermination of the indigenous. John Locke develops his political doctrines considering Natural Laws as the logical, metaphysical supposition. He assumes Natural Laws to be the logical presupposition, but is interested in North America. This is evidently seen in his works according to research outcomes. His 'possessive individualism' discusses exclusion and extermination, on the bound of natural laws and natural state. The person without possessive rights is excluded, the people without effective farming is forfeited. Then acculturation is the justifying of slavery and suggestive of extermination. In the possessive individualism of bourgeois society, that is, private property, man is annulled aboard. That is colonialism comes from, which destroys all the cultures but its own cultures. It is Locke who is the first thinker of the imperial. In the thought of Locke found we in profane terminology projected for the world imperial. After Locke, colonialism has been appeared in the guise of racism in the eighteen century, especially in the universal history of system of philosophy, sometimes in the face of orientalism on all sides. The ideas of colonialism and imperialism have been absolutely for the West. In the totally administered society nowadays, the hope of redemption has been made impossible from the origin. From the beneath, operated and practiced the program of deletion of race, its ethnic cleansing is a mere case. Locke's thought for the human rights is consisted of property and freedom in mankind, but it ground baits for its bloodied symposium with words and consults. 'Our word is our weapon', this is wording of one ethnic that is in nearing extermination.

• Journal of the Korea Society of Computer and Information
• /
• v.24 no.11
• /
• pp.127-133
• /
• 2019

SQL is the most common language in data processing. Therefore, most of the colleges offer SQL in their curriculum. In this research, an auto scoring SQL test is proposed for the efficient results of SQL education. The system was treated with algorithms instead of using expensive DBMS(Data Base Management System) for automatic scoring, and satisfactory results were produced. For this system, the test question bank was established out of 'personnel management' and 'academic management'. It provides users with different sets of test each time. Scoring was done by dividing tables into two sections. The one that does not change the table(select) and the other that actually changes the table(update, insert, delete). In the case of a search, the answer and response were executed at first and then the results were compared and processed, the user's answers are evaluated by comparing the table with the correct answer. Modification, insertion, and deletion of table actually changes the data table, so data was restored by using ROLLBACK command. This system was implemented and tested 772 times on the 88 students in Computer Information Division of our college. The results of the implementation show that the average scoring time for a test consisting of 10 questions is 0.052 seconds, and the performance of this system is distinguished considering that multiple responses cannot be processed at the same time by a human grader, we want to develop a problem system that takes into account the difficulty of the problem into account near future.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.18 no.5
• /
• pp.91-99
• /
• 2019

Existing node-to-node based optimal path searching is built on the assumption that all destination nodes can be arrived at from an origin node. However, the recent appearance of the adaptive path search algorithm has meant that the optimal path solution cannot be derived in node-to-node path search. In order to reflect transportation data at the links in real-time, the necessity of the node-to-link (or link-to-node; NL) problem is being recognized. This research assumes existence of a network with link-label and non-additive path costs as a solution to the node-to-link optimal path problem. At the intersections in which the link-label has a turn penalty, the network retains its shape. Non-additive path cost requires that M-similar paths be enumerated so that the ideal path can be ascertained. In this, the research proposes direction deletion and turn restriction so that regulation of the loop in the link-label entry-link-based network transformation method will ensure that an optimal solution is derived up until the final link. Using this method on a case study shows that the proposed method derives the optimal solution through learning. The research concludes by bringing to light the necessity of verification in large-scale networks.

• Journal of Preventive Medicine and Public Health
• /
• v.31 no.1 s.60
• /
• pp.15-26
• /
• 1998

p53 is the most frequently mutated gene in female breast cancer tissues and the prognosis of breast cancer could be changed by mutation of the gene. This study was performed to examine risk factors for breast cancer subtypes classified by p53 mutation and to investigate the roles of p53 gene mutation in carcinogenesis of breast cancer. The study subjects were 81 breast cancer patients and 121 controls who were matched to cases 1:1 or 1:2 age, residence, education level and menopausal status. All the subjects were interviewed by a well-trained nurse with standardized questionnaire on reproductive factors, and wire asked to fill the self-administrative food frequency questionnaire. p53 gene mutation in the cancer tissue was screened using polymerase chain reaction (PCR)-single strand conformational polymorphism (SSCP) method. Mutation type was identified by direct sequencing of the exon of which mobility shift was observed in SSCP analysis. Mutations were detected in p53 gene of 25 breast cancer tissues. By direct sequencing, base substitutions were found in 20 cancer tissues (10 transition and 10 transversion), and frame shift mutations in 5 (4 insertions and 1 deletion). For the whole cases and controls, risk of breast cancer incidence decreased when the parity increased, and increased when intake amount of total calory, fat, or protein increased. Eat and protein were statistically significant risk factors for breast cancer with p53 mutation. For breast cancer without p53 mutation, protein intake was the only significant dietary factor. These results suggest that causes of p53 positive breast lancer would be different from those of p53 negative cancer, and that dietary factors or related hormonal factors induce mutation of p53, which may be the first step of breast cancer development or a promoter following some unidentified genetic mutations.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.1
• /
• pp.391-395
• /
• 2014

Background: We aimed to evaluate the role of genetic polymorphisms in tobacco carcinogen-metabolizing genes and their interactions with smoking in a hospital-based case-control study of Japanese subjects. Materials and Methods: We examine the associations of pancreatic cancer risk with genetic polymorphisms in GSTM1, GSTT1 and GSTP1, phase II enzymes that catalyze the conjugation of toxic and carcinogenic electrophilic molecules. The study population consisted of 360 patients and 400 control subjects, who were recruited from several medical facilities in Japan. Unconditional logistic regression methods were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the associations between genotypes and pancreatic cancer risk. Results: Among the control subjects, the prevalence of the GSTM1-null genotype and the GSTT1-null genotype was approximately 56% and 48%, respectively. Cases and controls were comparable in terms of GSTM1 and GSTT1 genotype distributions. Neither of the deleted polymorphisms in GSTM1 and GSTT1 was associated with the risk of pancreatic cancer, with an age- and sex-adjusted OR of 0.99 (95%CI: 0.74-1.32) for the GSTM1-null genotype, and 0.98 (95%CI: 0.73-1.31) for the GSTT1-null genotype. The OR was 0.97 (95%CI: 0.64-1.47) for individuals with the GSTM1 and GSTT1-null genotypes compared with those with the GSTM1 and GSTT1- present genotypes. No synergistic effects of smoking or GST genotypes were observed. Conclusions: Our results indicate no overall association between the GSTM1 and GSTT1 deletion polymorphisms and pancreatic cancer risk in the Japanese subjects in our study.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.40 no.6
• /
• pp.140-147
• /
• 2012

This study was conducted to investigate optimum planting density for apartment complex. The validity of Landscape Architecture Criteria of Korea was checked for it. We compared our field data with Landscape Architecture Criteria. In this step, the tree density of urban forest was regarded as standard. Field study was examined in 3 apartment complexes located in capital area, especially completed during these 10 years. 10 sites in each complex were selected and tree density per unit area were calculated. This field study data was divided standard size and large size which received weight. And, it was compared and analyzed. And crown projected area(CPA) was calculated considering proper growth of low vegetation and sufficient shade. The outcome shows that minimum size of Landscape Architecture Criteria is rational. But, in the case of planting large size tree received weight, tree density was short comparing with the tree density of urban forest and CPA was less than 50%. By the result of field study in 3 apartment complex, the tree density of apartment complex satisfied or exceeded Landscape Architecture Criteria. But, in the case of planting large size tree, tree density and CPA show high density due to addition planting for deficient landscape. Therefore, the revision of the Landscape Architecture Criteria was required such as deletion or minimization of the weighted clause about the large size tree and regulate the limit CPA not less than 50% and not more than 100%.

• Journal of Genetic Medicine
• /
• v.7 no.2
• /
• pp.160-164
• /
• 2010

Structural abnormalities of the Y chromosome affect normal testicular differentiation and spermatogenesis. The present case showed a rare monocentric derivative Y chromosome with partial duplication of Yp including the SRY gene and deletion of Yq12 heterochromatin. The karyotype was 46,X,der(Y)(pter${\rightarrow}$q11.23::p11.2${\rightarrow}$pter).ish der(Y)(DYZ3+,DYZ1-,SRY++), confirmed through a FISH study. Even though the patient possessed an abnormal Y chromosome, testicular biopsy showed normal testicular volumes in the proband, with gonadal hormonal levels in the normal range but bilateral varicocele and hypospermatogenesis. We speculate that the abnormal Y chromosome arose from sister chromatids during Y chromosome recombination or intra chromosomal NAHR (non-allelic homologous recombination) during meiosis in the patient's father or in the very early stages of embryogenesis. The derivative Y chromosome might interfere in the meiotic stage of spermatogenesis, leading to the developmental arrest of germ cells. The present case illustrates that the infertility phenotype can have various causes. Also, it emphasizes the importance of accurate and various genetic analyses and could aid in male infertility treatment.

• Pediatric Infection and Vaccine
• /
• v.30 no.3
• /
• pp.173-179
• /
• 2023

Complete DiGeorge syndrome (cDGS) refers to DGS with profound T cell deficiency. Herein, we present the case of an infant with cDGS suffering from refractory cytomegalovirus (CMV) infection and who was treated with CD45RA+ depleted lymphocyte infusion. The patient was diagnosed with cDGS by fluorescence in situ hybridization which verified 22q11.2 deletion and as well as by the observed profound T cell deficiency (CD3+ T cells 69/μL, CD4+ T cells 7/μL). On the 45th day of age, CMV viremia was first detected with a plasma viral load (VL) of 120,000 IU/mL. Ganciclovir treatment effectively reduced VL post 56 days of treatment; however, VL subsequently rebounded. A CMV UL97 phosphotransferase M460V mutation conferring ganciclovir resistance emerged and foscarnet was incorporated. Despite this, high titers of CMV viremia (VL 2,820,000 IU/mL) and CMV retinitis were complicated. To restore T cell immunity and treat refractory CMV infection, CD45RA+ depleted CMV-specific lymphocytes from the patient's father were infused twice on the 196th and 207th days after birth. After receiving the second infusion, a decline in CMV VL was observed, with a decrease to 87,100 IU/mL by the tenth day following infusion, despite the failure in maintaining T cell increase. The patient died of Pneumocystis jirovecii pneumonia and Elizabethkingia meningoseptica sepsis on the 222nd day after birth. CD45RA+ depleted lymphocyte infusion may be a therapeutic option for refractory CMV disease in cDGS patients.