• Archives of Plastic Surgery
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• 제33권3호
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• pp.313-318
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• 2006

The preauricular fistula is a congenital malformation of the ear with a small opening in the preauricular area. In general, this malformation should be treated by excision after its infection is brought under control with antibiotics. For cosmetic consideration, we performed a elliptic incision around opening, and then we dissected along the fistula tract to the cyst without sacrificing too much soft tissues. From March 2001 to March 2005, 90 patients with 102 cases of fistulas were excised including a small portion of auricular perichondrium and cartilage, where they adhered closely. Then, histologic findings of preauricular fistula were studied. The histologic findings reveal that the fistular tract is very close to auricular cartilage, and the thickness of fistular epithelium and perichondrium are about the same. There was no specific complications related to this procedure. The recurrence rate for the excision with cartilage was 2 out of 102(2%). Results of surgery in all cases were satisfactory. It is important, in preauricular fistular excision, perichondrium and auricular cartilage should be excised to prevent recurrence.

• Archives of Plastic Surgery
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• 제39권6호
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• pp.601-605
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• 2012

Background Cryptotia is a congenital ear deformity in which the upper pole appears buried beneath the mastoid skin. Cryptotia is a common auricular malformation among Asians. The aim of this paper is to examine the surgical techniques for and complications of 34 cryptotic patients. Methods Surgery was performed for 34 cryptotic deformities (January 2005 to January 2012). Twenty-two patients (64.7%) were classified as having type I cryptotia, and 12 patients (37.5%) type II cryptotia. Among the type I cryptotia patients, 8 patients had mild deformity and 14 severe deformity. Among the type II cryptotia patients, 10 patients had mild deformity and 2 severe deformity. Results The mild deformities were corrected via Z-plasty, V-Y plasty, full-thickness skin graft, and transposition flap, while the severe deformities were corrected via cartilage graft or Medporfor the spread of cartilage adhesion of antihelix. There were two cases of reinvagination in the autologous cartilage graft group. Implant exposure occurred with Medpor (two cases). There were two cases of hypertrophic scar on the previous surgical wound with Medpor. There were no complications in the 18 patients who had mild deformities. Conclusions The type I cryptotia patients had more severe deformities than the type II cryptotia patients. As most of the type II cryptotia patients had only mild deformities, their deformities were corrected without using autologous conchal cartilage graft or Medpor, except for two patients. Through more case analyses, researchers should make an effort to identify methods for recurrence and prevention of complication.

• 한국통신학회논문지
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• 제39C권6호
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• pp.490-496
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• 2014

무릎 관절 연골은 두께가 얇아 대부분 무릎 질환의 원인이 되고 있다. 그러므로 무릎 자기공명영상에서 관절 연골 분할은 무릎 질환의 정확한 진단을 위한 필수조건이다. 특히 수동이 아닌 전자동 방식으로 무릎 관절 연골을 분할하여야만 효과적인 무릎 질환 진단을 할 수 있다. 본 논문에서는 뇌 자기공명영상에서 대표적으로 사용되는 레벨 셋 기반의 영상 분할 기법을 분석하여 무릎 자기공명영상에 적용 시 문제점을 파악하고 이를 해결함으로써, 무릎 자기공명영상에 레벨 셋 기반 영상분할 방식을 적용하였다. 이는 본 논문에서 제안하는 분할기법을 사용할 경우 무릎 관절 연골 분할에 대한 모든 과정이 전자동화 되어 기존 반자동화 방식보다 빠른 처리가 가능하며, 3차원 형상화를 통해 보다 정확한 진단에 도움을 줄 수 있다. 또한 우리는 제안하고 있는 분할기법이 기존 대표적인 무릎 관절 분할보다 더 높은 정확도를 갖는 것을 실험을 통해 확인할 수 있었다.

• Investigative Magnetic Resonance Imaging
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• 제8권2호
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• pp.100-108
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• 2004

목적 : Trypsin으로 관절연골의 변성을 인공적으로 유도한 뒤 관절연골의 구성성분인 glycosaminoglycan(GAG)의 소실을 생화학적으로 정량분석하고 $Gd(DTPA)^{2-}$-(gadolinium diethylene triamine pentaacetic acid)조영증강, 그리고 T1, T2, rho 이완시간등과 어떤 상관관계가 있는지를 조사함으로써 관절연골의 초기 퇴행성 변화에 대해서 자기공명영상(MRI)으로 관찰이 가능한 지를 알아보고자 하였다. 대상 및 방법 : 적출된 3개의 돼지 슬개골에서 실험에 사용될 관절연골조각을 폭 8 mm, 길이 10 mm 크기의 관절연골조각을 3개 만들었다. 분광광흡수계(spectrophotometry)에서 dimethylmethylene blue를 이용한 chondroitin sulfate standard의 흡수도를 측정하여 GAG을 정량분석 하였다. 관절연골을 배양할 용액은 0.2 mg/ml trypsin, 1 mM $Gd(DTPA)^{2-}$ 첨가된 trypsin, 그리고 대조군용 인산염완충식염수(phosphate buffered saline, PBS)의 3가지를 준비하였다. 배양시간은 1시간에서 5시간까지는 매시간마다 배양하였고 24시간과 48시간까지 배양하였다. MRI촬영은 1시간에서 5시간까지만 매시간마다 배양후 시행하였다. 1.5T 기기에서 MRI촬영은 T1강조영상(TR/TE, 450/22)과 혼합에코기법(mixed echo sequence) (TR/TE, 760/21-168, 8 echoes)등을 시행하였다. 모든 촬영에서 영상영역은 50 mm, 절편두께는 2 mm, 그리고 매트릭스는 $256\times512$ 였다. MRI촬영자료에서 관절연골부위를 픽셀단위로 비교분석 하였다. 배양이 끝난 관절연골은 hematoxylin & eosin, toluidine blue, alcian blue, trichrome 염색 등을 시행하여 관찰하였다. 결과 : Dimethylmethylene blue를 이용한 GAG의 정량분석결과 배양시간증가에 따라 GAG의 농도가 비례적으로 증가하였다. $Gd(DTPA)^{2-}$ 첨가된 trypsin배양에서 관절연골의 T1 강조 영상에서의 신호강도는 trypsin 배양에 비하여 평균 $42.0\%$ 증가하였고 4시간과 5시간배양에서는 trypsin에서만 배양한 관절연골에 비하여 신호강도가 더욱 뚜렷하게 증가되었다 (p<0.05). 관절연골의 T1, T2, rho 이완시간은 배양시간에 따라 유의성 있는 차이가 관찰되지 않았으나 $Gd(DTPA)^{2-}$ 첨가된 trypsin배양에서 T1이완시간의 증가가 관절연골의 표층부와 이행부에서 측정되었다. 조직검사결과 trypsin 배양의 관절연골에서 toluidine blue와 alcian blue염색에서 결손이 관찰되었다. 결론 : Trypsin 배양시간에 따라 관절연골의 GAG결손을 정량적으로 확인할 수 있었고 픽셀크기 $97.9\times195\;{\mu}m$인 MRI로 $Gd(DTPA)^{2-}$-조영증강 및 이완시간을 측정할 수 있었다. 배양시간에 따른 GAG결손은 T1, T2, rho 이완시간보다 $Gd(DTPA)^{2-}$-조영증강에서 유의한 차이를 관찰할 수 있었다. 그러므로 관절연골의 초기 퇴행성 변화를 진단하는 데는 T1강조영상에서 $Gd(DTPA)^{2-}$-조영증강정도를 비교하는 것이 가장 유용할 것으로 사료된다.

• 대한족부족관절학회지
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• 제10권2호
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• pp.117-124
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• 2006

Purpose: This study was aimed at elucidating the pathogenesis of talar osteochondral lesion by analyzing the histopathological findings. Materials and Methods: Twenty specimens from 20 patients who underwent surgical treatment for talus osteochondral lesions were studied. Preoperative MRI images including T1, T2, and stir images were taken and cases were classified according to modification of the Anderson's classification. There were 5 cases of MRI group 1, 6 cases of group 2, 7 cases of group 3 and 2 cases of group 4. A full thickness osteochondral plug including the osteochondral lesion of the talus was harvested from each patient and reviewed histopathologic changes of osteochondral fragment using H-E staining. Mean diameter of specimens was 8.5 mm and mean depth was 10.3 mm. Pathologic changes of articular cartilage and subchondral bone were observed. Subchondral bone was divided into superficial, middle and deep zones according to depth. Cartilage formation, trabecular thickening and marrow fibrosis were observed in each zone. Results: There were detachment of the joint cartilage at the tidemark in 16 cases of 20 cases and the separated cartilages were almost necrotic on the histopathologic findings. Cartilage formation within subchondral bone was discovered beneath the tidemark in 12 cases. Trabeculae were increased and thickened in 17 cases. These pathologic changes were similar to fracture healing process and these findings were more conspicuous near the tidemark and showed transition to normal bone marrow tissue with depth. No correlation between the pathological progression and MRI stages was found. A large cyst shown on MRI's was microscopically turned out to be multiple micro-cysts accompanied by fibrovascular structure and newly formed cartilage tissue. Conclusion: The histopathologic findings of osteochondral lesions are detachment of overlying cartilage at the tidemark and subsequent changes of subchondral bone. Subchondral bone changes are summarized as cartilage formation, marrow fibrosis and trabecular thickening that mean healing process following repeated micro fractures of trabecular. These osteochondral lesions should have differed from osteochondral fractures.

• Journal of Pharmaceutical Investigation
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• 제40권1호
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• pp.45-49
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• 2010

Unlike human, with some exceptions, animals do not heal with excessive scar. The lack of suitable animal model has hindered the development of effective scar therapy. We previously reported that partial thickness rabbit ear wound model resembles human wound heal process. This study was designed to prepare a hypertropic scar wound model which can be employed for testing anti-scar therapy. Four wounds were created down to the bare cartilage on the anterior side of each rabbit ear using 8-mm dermal biopsy punch and histology analysis at post operation day (POD) 5, 28 and 48 were performed. As the outcome of scar formation is largely determined by the early inflammatory response to the wounding and the degree and the duration of occlusion, cephalodin(50 mg/kg) was injected daily and medical occlusive dressings were applied. Five micro wound and scar sections were stained with hematoxylin and eosin for quantification of epidermal regeneration and scar hypertrophy. Sections were also stained using Masson's trichrome and Sirius red to evaluate collagen organization and rete ridge formation. Wound closure process was assessed to 7wks post wounding. Complete removal of the epidermis, dermis and perichondrial layer caused delayed epithelialization, which results in hypertropic scarring. The inability of the wounds to contract and the delay in epithelialization in rabbit ear was likely due to cartilage and it created scar elevation. The results suggest that full thickness surgical punch wound model in rabbit ear could be employed as a reliable and reproducible scar wound model for testing anti-scar therapy.

• 대한치과교정학회지
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• 제19권3호
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• pp.15-33
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• 1989

The purpose of this study was to investigate changes of the mandible of a growing rat when they are subjected to a retractive force and those after removal of the retractive force. The experimental animals were Sprague-Dawley male rats of four weeks of age. A mandible was retracted with 50 grams of force on each side in the posterior and superior direction for 8 hours per day. The animals were sacrificed after 1 week, 2-week and 4-week force application, and after 4-week force application-4-week force removal period. The changes of rat mandibular growth following retractive force on the growing rat mandible were observed histologically and biometrically. The findings were as follows ; 1. Histologically, the thickness of the condylar cartilage was slightly reduced in the anterosuperior region with the retractive force. However, in the group of 4-week force application-4-week force removal, there was no significant difference in the thickness of the condylar cartilage. 2. There were no significant histological changes in the articular disk and glenoid fossa through the experimental period. 3. The length and anterior height of the mandible subjected to the retractive force were significantly smaller and greater than those of the control group. 4. There were no significant differences in the mandibular length between 4-week force application - 4-week force removal and the control group. 5. It was concluded that a mandibular retractive force produced inhibitory effects in the growth of the mandible, but that these effects were not sustained during mandibular growth in this experimental model.

• 대한두개안면성형외과학회지
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• 제15권3호
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• pp.133-137
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• 2014

Reconstruction of a full-thickness alar defect requires independent blood supplies to the inner and outer surfaces. Because of this, secondary operations are commonly needed for the division of skin flap from its origin. Here, we report a single-stage reconstruction of full-thickness alar defect, which was made possible by the use of a nasolabial island flap and septal mucosal hinge flap. A 49-year-old female had presented with a squamous cell carcinoma of the right ala which was invading through the mucosa. The lesion was excised with a 5-mm free margin through the full-thickness of ala. The lining and cartilage was restored using a septal mucosa hinge flap and a conchal cartilage from the ipsilateral ear. The superficial surface was covered with a nasolabial island flap based on a perforator from the angular artery. The three separate tissue layers were reconstructed as a single subunit, and no secondary operations were necessary. Single-stage reconstruction of the alar subunit was made possible by the use of a nasolabial island flap and septal mucosal hinge flap. Further studies are needed to compare long-term outcomes following single-stage and multi-stage reconstructions.

• Investigative Magnetic Resonance Imaging
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• 제3권1호
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• pp.66-72
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• 1999

목적: 급속 스핀 에코 MR영상과 Dual Echo in Steady State(DESS) 영상을 비교하여 슬관절 연골 연화증의 병소 발견에 있어서 DESS 영상의 유용성을 알아 보고자 하였다. 대상 및 방법: 1997년 1월부터 1998년 7월까지 관절경 수술과 자기 공명 영상을 시해안 환자중 수술 소견에서 슬관절의 연골 연화가 있었던 26명(남:여=14:12, 평균 46.3세)을 대상으로 하였다. 1.5 T 자기 공명 장치를 사용하였고, 급속 스핀 에코 양자 밀도와 T2 강조영상의 게측 게수로써 TR/TE 3000-4200/16-96msec, FOV $140-160{\times}150-160$, matrix size $180{\times}256$, 절편 두께 4.0mm, 절편 간격은 0.5mm로 하였으며, DESS 영상의 계측계수로써 TR/TE 25.4/9.0msec, filp angle $35-35^{\circ}$, FOV $150-160{\times}150-160mm$, matrix size $192{\times}256$, 효과적 절편 두께 1.5mm로 하였으며, DESS 영상은 시상단면을, 양자 밀도 강조 영상과 T2 강조영상은 시상 단면과 관상 단면을 얻어 비교하였다. 영상의 분석은 2명의 방사선과 의사가 합의하여 후향적으로 분석하였고, 슬개-대퇴 구획, 슬관절의 내측, 외측 구획으로 나누었으며, 자기 공명 영상과 관절경 소견의 등급은 0: 정상, I: 연화 또는 수포, II: 표층 균열, III: 심층 균열, IV: 전층 균열 또는 골노출로 나누었고, 각각의 민감도, 특이도, 그리고 정확도를 구하였다. 결과: 관절경 소견상 I등급 7예, II등급 21예, III등급 6예, IV등급은 18예 였으며, 급속 스핀 에코 자기공명 영상에서의 관절경 소견과 구획과 등급이 일치했던 경우는 등급별로 0%, 14%, 0%, 61%로 나타났으며, DESS 영상에서는 각각 0%, 33%, 50%, 67% 이었다(p=0.001). 또한 각 부위에서 등급을 고려하지 않은 경우, 고식적 자기공명 영상에서의 민감도, 특이도, 그리고 정확도는 59.6%, 88.6%, 78.8% 였으며, DESS 영상에서는 73.1%, 88.4%, 82.2%로 나타났다(p=0.007). 결론: 슬관절의 연골 연화증의 진단에 있어서 DESS 영상이 급속 스핀 에코 자기공명영상보다 높은 발견율과 민감도를 보였다. 그러므로 급속 스핀 에코 MR 영상과 더불어 DESS 영상이 슬관절 연골연화즈의 진단에 보다 더 도움을 줄 것으로 생각한다.

• 한국임상수의학회지
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• 제18권2호
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• pp.116-123
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• 2001

This study was undertaken to find out the effect of Korean safflower seed powder on histopathological changes of cadmium toxicity in mice. Fifty BALB/c mice were divided into a control group(A) and four experimental groups(B, C, D, E) : group A received tap water and basal diet, group B received tap water and diet supplemented with 3% Korean safflower seed powder alone, group C received basal diet and 300 $\mu\textrm{g}$/g of cadmium, group D and E received basal diet supplemented with 3% and 10% Korean safflower seed powder and 300$\mu\textrm{g}$/g of cadmium respectively. Cadmium dissolved in tap water was used, and the Korean safflower seed powder were mixed with feed. All mice were dissected on the 56th day. Histopathological changes in liver, kidney, lung, cortical osseous tissue of femoral shaft, bone trabecular of femur, and epiphyseal cartilage plate of femur were observed. Group B showed no significant changes compared with the control group. But group C showed the unclearness of specific cells in liver, the loss of architecture and focal necrosis of hepatocyte, the glomerular swelling, degeneration and necrosis of convoluted tubules, desquamation and vacuolization of the greater part of the renal tubular epithelium, the marked congestion and thickness of the wall of alveolus in lung, slightly thinning of the cortical osseous tissue in femoral shaft, reduction of cancellous bone volume and marked narrowness of bone trabecular, marked thinning of epiphyseal cartilage plate and irregular arrangement of columnar structure of cartilage cells. On the other hand, Korean safflower seed powder-treated group showed a little convalescent changes and maintained their normal architectures in liner, kidney, lung, cortical osseous tissue of femoral shaft, bone trabecular of femur and epiphyseal cartilage plate of femur.