• The Journal of Korean Medicine
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• v.16 no.1 s.29
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• pp.379-402
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• 1995

This study was performed to investigate the comparative effects of Apitoxin(0.1% bee venom 0.1cc) & Acupuncture therapy on the knee joint arthritis induced by 0.1% carrageenin solution at Rt. knee joint in rats. After carrageenin injection, the apitoxin was injected for two times (Experimental group I : 1 & 24hours later, Experimental group II : 48 & 72hours later) into the corresponding loci to Rt. $ST_{35}$(Group 3) & $EX-LE_4$(group 4) of the human body in rats. Acupuncture therapy was done same as above. And then the comparative effects of apitoxin and acupuncture therapy on the knee joint arthritis were estimated by the WBC count, RBC count, hemoglobin level, hematocrit level and ASO titer in serum. The results were summerized as follows : 1. The effects of apitoxin & acupuncture on WBC count showed remarkable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 2. The effect of apitoxin on RBC count showed noticeable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. The acupuncture was not showed decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 3. The effects of apitoxin & acupuncture on hemoglobin level showed noticeable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 4. The effect of apitoxin on hematocrit level showed noticeable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. The acupuncture was not showed decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 5. The effect of apitoxin on ASO titer showed noticeable decrease at group 3 & group 4 as compared with the control group only in experimental group I. The apitoxin & acupuncture showed decrease at group 3 & group 4 as compared with the control group in experimental group II. There were significantly statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. The effect of apitoxin showed slightly decrease as compared with the acupuncture therapy by Duncan's multiple range test.

• The Korean Journal of Pharmacology
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• v.23 no.1
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• pp.51-56
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• 1987

Cyclobuxine D was extracted from Buxus microphylla var. koreana Nakai. The effects of cyclobuxine D on the biosynthesis of prostaglandins from arachidonic acid in guinea pig lung, prostaglandin production and leukocyte migration in carrageenin-induced inflammation was investigated. These effects of cyclobuxine D were compared with those of aspirin and dexamethasone. Cyclobuxine D does not inhibit significantly cyclooxygenase in guinea pig lung but reduces prostaglandin concentration and leukocyte migration in inflammatory exudates. These effects of cyclobuxine D differ from that of aspirin which inhibits biosynthesis of prostaglandin in vitro and has a relative small effect on leukocyte migration. Dexamethasone, which does not inhibit cyclooxygenase in vitro, has an effect similar to that of cyclobuxine D on leukocyte migration and prostaglandin production in inflammatory exudates.

• YAKHAK HOEJI
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• v.15 no.3_4
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• pp.99-102
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• 1971

상륙(Phytolacca esculenta)에서 새로운 물질 trihydroxyolean-12-ene dicarboxylic acid, $C_{30}H_{46}O_{7}$, m.p.318-$320^{\circ}$, [$\alpha$]$_D$$^{6}$ = +113.$7^{\circ}$를 분리하고 jaligonic acid라고 명명하였다. 본물질은 carrageenin으로 유발시킨 edema형성을 강력하게 억제하였다.

• Korean Journal of Pharmacognosy
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• v.16 no.1
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• pp.18-25
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• 1985

Experimental studies were conducted to investigate for the effect of Tongkyu-tang on analgesic, antipyretic, sedative, anti-inflammatory, isolated ileum and anti-histamine actions. Tongkyu-Tang was composed of sixteen crude drugs including Astragali Radix, which has been used for the treatment of common cold, headache, rhinitis, nasal obstruction, rhincospasm, etc. The following results have been obtained; analgesic and antipyretic actions were obtained. Prolonged action against the hypnotic duration of pentobarbital were significantly noted in mice. Anti-inflammatory effects in the paw edema induced by 1% carrageenin and 1% dextran were significantly shown in rats. Spontaneous motilities of isolated ileum of mice were strongly suppressed, and contractions of isolated ileum of mice and guinea-pigs induced by acetylcholine chloride, barium chloride and histamine were remarkably inhibited. Antihistamine actions were noted. Considering the above experimental results, it is suggested that effects of Tongkyu-tang based on the Oriental Medical References were similarly consistent with the actual experimental results.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.18-24
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• 1999

This study was investigated on anti-inflammatory and analgesic activity on Gu-Mi-Gang-Hwal-Tang which has been used for the treatment of common cold and headache, arthralgia, fever in oriental medicine. The anti-inflammatory activities of Gu-Mi-Gang-Hwal-Tang water extract (GMGHT) on the carrageenin induced edema, acetic acid induced edema, and corton oil induced granuloma pouch in rats were examined. The analgesic effect of the GMGHT was investigated utilizing acetic acid induced writhing syndrome in mice. The antipyretic activity of the GMGHT was also investigated. The oral administration of GMGHT has been shown the anti-inflammatory activities in 1% carrageenin and acetic acid induced edema in rats. The GMGHT has been shown significant inhibitory effects on glanuloma and exudate formation in rats, especially the GMGHT (450 mg/kg) has the efficacy more than the GMGHT (150 mg/kg). The writhing syndromes induced by acetic acid in mice were inhibited, especially analgesic activity of the GMGHT (450 mg/kg) is similar to that of piroxicam and antipyretic activities were recognized in rats. In the present study, the GMGHT is indicated to have the anti-inflammatory, analgesic and antipyretic activities.

• Korean Journal of Pharmacognosy
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• v.32 no.3 s.126
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• pp.242-247
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• 2001

The anti-inflammatory activity of water extract of Mahaengeuigam-Tang(MHEGTWE) was examined using the carrageenin and acetic acid induced edema, croton oil induced granuloma pouch, and adjuvant arthritis in rats. In addition, the acute toxicity, analgesic and antipyretic effects of MHEGTWE were investigated by the general experimental methods. In acute toxicity test in mice, MHEGTWE showed 10% mortality at 2400 mg/kg(p.o), but it did not showed at 1200 mg/kg(i.p). It was also showed significant analgesic action on the writhing syndrome induced by 0.7% acetic acid at 600 mg/kg(p.o) and its antipyretic activity was observed in the typhoid vaccine induced fevered rats at 300 mg/kg(p.o). By the oral administration of the MHEGTWE, the significant anti-inflammatory activity was observed on 1% carrageenin induced edema, and it significantly inhibited the granuloma and exudate formation in rats. In the adjuvant arthritis experiment, the MHEGTWE decreased the hind paw edema in rats for 19 days. The results suggest that MHEGTWE has analgesic, anti-inflammatory and antipyretic action.

• Korean Journal of Pharmacognosy
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• v.29 no.2
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• pp.86-92
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• 1998

Chang-Back-San and Chil-Mi-Chang-Back-San have been used for the treatment of neuralgia and arthritis in traditional medicine. The anti-inflammatory activities of Chang-Back-San and Chil-Mi-Chang-Back-San water extract (CBSE and CCBSE) on the carrageenin induced edema, Corton oil induced granuloma pouch, and adjuvant arthritis in rats were examined. The analgesic effects of the CBSE and CCBSE were also investigated utilizing acetic acid induced writhing syndrome in mice. The oral administration of CBSE and CCBSE showed to have the anti-inflammatory activities in 1% carrageenin induced edema in rats. They also showed significant inhibitory effects on granuloma and exudate formation in rats. In the method of adjuvant arthritis, they orally administered for 19 days, inhibited the hind paw edema in rats from 3rd day to 19th day, especially CCBSE has the efficacy more than CBSE. They significantly decreased the number of writhing syndromes induced by acetic acid in mice. In the present study, CBSE and CCBSE were indicated to have the anti-inflammatory and analgesic activities.

• Herbal Formula Science
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• v.18 no.1
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• pp.87-94
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• 2010

Objectives : To provide the information of efficacy for Sagunja-tang (Sijunzi-tang; SG), it was evaluated the anti-inflammatory effect. SG, a widely used herbal formula in tranditional Korean medicine, has been used to treat for the Boki-invigorating. In many studies, plant-derived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of SG extract on LPS-stimulated RAW 264.7 cells. Methods : The productions of nitric oxide (NO), prostaglandin (PG)$E_2$, interleukin (IL)-6 and tumor necrosis factor (TNF)-$\alpha$ were examined in a macrophage cell line, RAW 264.7 cells, in the presence of the SG extract. RAW 264.7 cells were incubated with LPS $1\;{\mu}g/mL$ and SG extract for 18 hours. The anti-inflammatory activity of SG was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 0, 2 and 4 hours following carrageenin-induced paw edema in rats. Results : SG extract showed inhibitory effect on $PGE_2$, IL-6 and TNF-$\alpha$ by LPS-stimulated RAW 264.7 cells. But SG extract was not inhibitory effect on NO by LPS-stimulated RAW 264.7 cells. And administration of SG extract (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : These results suggest that SG extract has anti-inflammatory activities in vitro and in vivo models.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.41-46
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• 2011

Objectives : To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated. Methods : A quantitative analysis was performed using a high performance liquid chromatography (HPLC). Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO) and prostaglandin $(PE)E_2$ were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats. Results : The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9992) over the linear range. The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41-101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on $PGE_2$ production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.

• The Journal of Korean Obstetrics and Gynecology
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• v.23 no.3
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• pp.101-111
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• 2010

Purpose: To provide the information of efficacy for herbal formulas of high frequency, it was evaluated the anti-inflammatory effect. In many studies, plantderived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of herbal formulas of high frequency on LPS-stimulated RAW 264.7 cells. Methods: Anti-inflammatory activity was investigated in 25 herbal formula extracts in vitro and in vivo. To investigate the anti-inflammatory effect in vitro model, using LPS-stimulated macrophages, RAW 264.7 cell line. The productions of nitric oxide(NO), prostaglandin(PG)$E_2$, interleukin(IL)-6 and tumor necrosis factor(TNF)-$\alpha$ were examined in RAW 264.7 cells, in the presence of the herbal formulas. RAW 264.7 cells were incubated with LPS $1\;{\mu}g/mL$ and herbal formulas for 18 hours. As an in vivo, using a rat model of carrageenin-induced paw edema. The paw volume was measured at 2 and 4 hours following carrageenin-induced paw edema in rats. Results: 8 kinds of herbal formula inhibited NO production by LPS-stimulated in some concentration, but the effect of NO inhibition is weak. 12 kinds of herbal formula inhibited $PGE_2$ production by LPS-stimulated over the 30%. Among them Gumiganghwal-tang, Sagunja-tang, Samchulkunbi-tang, Insampaedok-san and Hwangryunhaedok-tang inhibited IL-6 production by LPS-stimulated but TNF-$\alpha$ was not inhibited. 12 kinds of herbal formula reduced the carrageenin-induced paw edema in rats. Particularly, 3 kinds of herbal formula(Gumiganghwal-tang, Ssanghwa-tang and Soshiho-tang) were better than indomethacin. Conclusion: These results suggest that Gumiganghwal-tang, Sangunja-tang, Samchulkunbi-tang, Insampaedok-san and Hwangryunhaedok-tang have antiinflammatory activity.