• 농약과학회지
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• 제4권2호
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• pp.29-31
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• 2000

5,6-디히드로-2-메틸-1,4-옥사티인-3-카르복스아닐리드 (1, 카르복신)의 간편하고 경제적인 새로운 합성법과 그 반응 메카니즘을 제시하였다. 아세토아세트아닐리드의 알파-염화물 4 (1당량), 2-메르캡토에탄올 (1.2당량)을 산촉매 파라톨루엔술폰산 일수화물 (0.05당량) 존재하에서 톨루엔 용액 중에서 가열 환류하면서 탈수하여 카르복신 1을 합성하였다. 염화물 4의 헤미티오케탈인 알파 클로로 1,3-옥사티올란 유도체 8의 황원자의 인접기 참여에 의해서 분리할 수 없는 술포니움 이온 9로 전환되고 이어서 보다 안정한 옥소니움 12로 전환되고 이어서 양성자가 제거되어 카르복신 1이 생성됨을 제안하였다.

• 대한화학회지
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• 제25권1호
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• pp.44-49
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• 1981

Acetoacetanilide를 브롬화한 결과 예상한 ${\alpha}$-bromo 유도체가 얻어지지 않고, r-bromo 유도체가 얻어졌다. 이것을 mercaptoethanol과 반응시켜 sulfide 유도체를 만든다음, 산성하에서 고리화시킨 결과 2-(N-phenylcarbamoyl)methylene-1,4-oxathiane이 좋은 수율로 얻어졌다. 이 물질은 살균제 농약으로 널리 사용되고 있는 carboxin과 그 구조가 매우 유사하므로 이 물질의 biological activity는 흥미있는 과제가 될 것이다.

• 농약과학회지
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• 제9권3호
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• pp.185-190
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• 2005

최초의 침투이행성 농약 살균제, 카르복신 1의 유도체인 16을 고체상에서 합성하였다. 1,3-옥사티올란 유도체를 아실화 반응성을 갖고 있는 4-하이드록시-3-나이트로벤조페논 고체상 6에 연결하여 9를 82%의 수율로 합성하였다. 고체상의 1,3-옥사티올란 9의 황원자를 MCPBA로 산화한 다음 생성된 설폭사이드 10을 산촉매 존재하에서 환팽창하여 상응하는 고체상에 연결된 다이하이드로-1,4-옥사티인 유도체 12를 합성하였다. 고체상의 1,3-옥사티올란 9의 산화물을 p-메톡시아닐린과 반응시켜 1,3-옥사티올란 14, 1,3-옥사티올란 설폰 15, 다이하이드로-1,4-옥사티인 16, 그리고 아세토아세트아닐라이드 유도체 17을 각각 41%, 35%, 14%, 10% 수율로 얻었다.

• Plant Resources
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• 제8권2호
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• pp.130-134
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• 2005

This study was conducted to evaluate the control effect of fungicides on control of Gray mold, growth characteristics, and root yield in the cultivation of Scutellaria baicalensis after barley cropping. All fungicides treated had no effect on the growth and flowering rate of Scutellaria baicalensis. All seed disinfectants had no effect on the growth and flowering date of Scutellaria baicalensis Georg. The major seed disinfectants were Benomyl Wp, $20\%$, Captan Wp, $50\%$, Hymexazol Ec, $30\%$, Carboxin D, $37.5\%$. Dry root yield were increased largely with Benomyl Wp, $20\%$, seed disinfectant than the other seed disinfectants had no injury with standard dosage. On the other hand, all seed disinfectants had slight injury in the double dosage level for the Scutellaria baicalensis Georg.

• Journal of Microbiology and Biotechnology
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• 제19권3호
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• pp.271-276
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• 2009

Functional gene studies in the cultivated white button mushroom Agaricus bisporus have been constrained by the absence of effective gene-silencing tools. Using two endogenous genes from A. bisporus, we have tested the utility of dsRNA hairpin constructs to mediate downregulation of specific genes. Hairpin constructs for genes encoding orotidine 5'-monophosphate decarboxylase (URA3) and carboxin resistance (CBX) were introduced into A. bisporus using Agrobacteriummediated transfection. Although predicted changes in phenotype were not observed in vitro, quantitative-PCR analyses indicated unambiguously that transcripts in several transformants were substantially reduced compared with the non-transformed controls. Interestingly, some hairpin transformants exhibited increased transcription of target genes. Our observations show that hairpin transgenic sequences can mediate downregulation of A. bisporus endogenous genes and that the technology has the potential to expedite functional genomics of the mushroom.

• 한국약용작물학회지
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• 제23권6호
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• pp.460-467
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• 2015

Background : The aim of the present study was to identify an effective physicochemical control method to reduce Fusarium species infestation in adlay (Coix lacryma-jobi L.) before and after harvesting. Methods and Results : We observed that prochloraz emusifiable concentrate and hexaconazol prochloraz emusifiable concentrate strongly inhibited the mycelial growth of 10 Fusarium species. Strong growth inhibitions and cell lysis were observed following treatment with 4% NaOCl solution. The total number of fungi detected were lower follwing treatment with thiophanatemethyl triflumizole wettable powder ($1.1{\times}10^4CFU/g$), hexaconazol prochloraz emulsifiable concentrate ($1.2{\times}10^4CFU/g$), carboxin thiram dustable powder ($1.6{\times}10^4CFU/g$) and prochloraz emulsifiable concentrate ($1.7{\times}10^4CFU/g$) than in the non-treated control ($7.7{\times}10^4CFU/g$). The reduction of Fusarium fungi varies with the concentration and soaking time of NaOCl solution. Fungal detection was not observed after soaking in NaOCl solution for 24 h and harmful effects were not observed for plant growth by NaOCl after soacking for 6 - 12 h. Conclusion : Soaking seed for 6 - 12 h in 4% NaOCl could be an effective method of disinfectant treatment for the control of Fusarium fungi in adlay seeds.