• Title/Summary/Keyword: carbohydrates

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DETERMINATION OF AMINO ACIDS IN RICE(PALDAL) BY IONEXCHANGE CHROMATOGRAPHY

  • Lee, Chun-Yung;Lee, Tae-Yung;Kwon, Tai-Wan
    • Applied Biological Chemistry
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    • v.2
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    • pp.41-44
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    • 1961
  • The amino acid analysis of ground rice and the crude protein isolated from rice were performed by the most recent method of Moore, Spackman and Stein, using Amberlite IR-120. The effect of carbohydrates on the hydrolysis of rice was found to be definite in losing several amino acids nevertheless Dustin's claim that large excess of hydrolyzing acid reduces the losses of amino acid. Consequantly the removal carbohydrates as much as possible is considered to facilitate the amino acid determination. The amino acid composition of the crude rotein of rice was very similar to that previously found by other investigators.

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Observations on some mangrove-associated algae from the western Pacific (Guam, Chuuk, Kosrae, and Pohnpei)

  • West, John A.;Kamiya, Mitsunobu;Loiseaux de Goer, Susan;Karsten, Ulf;Zuccarello, Giuseppe C.
    • ALGAE
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    • v.28 no.3
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    • pp.241-266
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    • 2013
  • The mangrove algal flora of Guam and the Federated States of Micronesia has been poorly explored. We add to our knowledge of this region by observations of collections from these regions. This paper presents new and additional records of: Rhodophyta-Acrochaetium globosum, Colaconema sp., Caulacanthus indicus, Bostrychia moritziana / B. radicans, B. radicosa, B. simpliciuscula, B. kelanensis and B. tenella, Murrayella periclados, and Caloglossa ogasawaraensis; Chlorophyta-Boodleopsis carolinensis; and Phaeophyceae-Dictyota adnata, Dictyotopsis propagulifera, and Canistrocarpus cervicornis. Most specimens were cultured to investigate their reproductive biology and many specimens were further identified using molecular data. Low molecular weight carbohydrates (dulcitol, sorbitol, and digeneaside) were identified in samples of B. radicosa and B. simpliciuscula. We also present data on manganese-rich deposits found on B. simpliciuscula and B. tenella in culture, possibly formed by epiphytic bacteria.

Red Algae Pulp and Its Use in Papermaking

  • Seo, Yung-Bum;Lee, Youn-Woo;Lee, Chun-Han;You, Hack-Chul;Boo, Sung-Min
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.06a
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    • pp.153-159
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    • 2006
  • Red algae were used to make bleached pulp, from which paper handsheet samples were made. Red algae consists of rhizoidal filaments, epidermal tissues whose color were reddish, mucous carbohydrates, and other minor elements. Rhizoidal filaments of high brightness were obtained after extracting out mucous carbohydrates, and bleaching the remainder by using bleaching chemicals. The sizes and shapes of several rhizoidal filaments (or red algae pulp) from different red algae species were examined, and their handsheet properties were compared. Transparent and transluscent high density paper samples were made without applying refining process from the red algae pulp. White paper samples with good printability and excellent formation were made. We are developing pilot scale pulping and papermaking facilities at this time.

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Effect of $Co^60$ $\gamma$-Ray on Salmonella typhi, Ty2 cell (Salmomella typhi, Ty 2주에 미치는 전리방사선의 영향)

  • 이강순;민봉희;장정순
    • Korean Journal of Microbiology
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    • v.11 no.2
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    • pp.79-88
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    • 1973
  • In order to investigate the effect of gamma radiation on Salmonella typhi, Ty2, the components of amino acids, proteins, carbohydrates and lipids in irradiated cells were compared with those in unirradiated control cells respectively. The results obtained were as follows ; 1) The inactivation curves of Salmonella typhi with $Co^{60}$ .gamma.-ray irradiation were exponential over a wide range to the irradiated doses. 2) Dose for the inactivation factor of $10^8$ was 94.0 Krad in physiological saline or in phosphate buffered saline, 104.2 Krad in nutrient broth, 220.4Krad in frozen state, 552.0 Krad in dried state, 88.3 Krad in the abundance of oxygen and 188.0 Krad in the deficience of oxygen, respectively. 3) Five consecutive irradiation of Salmonella typhi suspension at the dose of 90 Krad gave no additional increase in resistance. 4) Even at the smallest dose of 500 Krad, compositions of amino acids, proteins, carbohydrates, and lipids were more or less decreased and the distinct banding patterns were also lost possibly due to degradation of the protein molecules.

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Lectin-binding properties of chicken primordial germ cells during embryonic development

  • Kim, Duk-Kyung;Seo, Sam-Youl;Lee, Eun-Young;Lee, Seul-Ki;Han, Jae-Yong
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2001.11a
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    • pp.69-70
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    • 2001
  • Lectins have great potential as to determine the alternation of the distribution of cell surface carbohydrates during cellular development and differentiation. Here, we investigated the presence and distribution of cell surface carbohydrates on chicken primordial germ cells (PGCs) during the migration and gonadal stages using a variety of lectins. A total of six FITC-labelled lectins from several specificity classes were used: ConA (glucose/mannose), WGA (N-acetylglucosamine), STA (N-acetylglucosamine), DBA (N-acetylgalactosamine/galactose), UEA-I (fucose) and PHA-E (oilgosaccharide). As a results, PGC-specific binding was observed in STA. PGCs of migration stage (2.5- and 5.5-day embyos) were STA-positive whereas PGCs of 10-day embryonic gonad were not. The results suggest that N-acetylglucosamine residuse are present specifically in migrating chicken PGCs and changes during development.

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Detection of Small Neutral Carbohydrates Using Various Supporting Materials in Laser Desorption/Ionization Mass Spectrometry

  • Yang, Hyo-Jik;Lee, Ae-Ra;Lee, Myung-Ki;Kim, Woong;Kim, Jeong-Kwon
    • Bulletin of the Korean Chemical Society
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    • v.31 no.1
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    • pp.35-40
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    • 2010
  • A comprehensive comparative investigation of small carbohydrates in laser desorption ionization was performed on supporting materials composed of sodiated 2,5-dihydroxybenzoic acid (DHB), carbon nanotubes, an ionic liquid matrix of DHB-pyridine, a binary matrix of DHB-aminopyrazine, zinc oxide nanoparticles, and gold nanoparticles. The abundance of $[M+Na]^+$ ions, where M is glucose or sucrose, was compared for each supporting material. The highest sensitivity for both glucose and sucrose, with a detection limit of 3 pmol, was observed with carbon nanotubes. Both carbon nanotubes and the ionic liquid matrix exhibited the highest reproducibility.

Induction of Melibiase in Yeast

  • Park, Sang-Shin
    • Journal of Plant Biology
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    • v.7 no.3
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    • pp.1-8
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    • 1964
  • Exposing yeast cells with a certain genotype to different inducers, the ability of the yeast cells (Saccharomyces cerevisiae) to obtain enhanced fermentation for carbohydrates was observed. Regardless of the preexposure to any substrate, the inherent character incapable of fermenting a certain carbohydrate was maintained, while utilization of carbohydrates by the cells with a certain gene markers was varied by the previous conditions where they were exposed. Galactose was the best inducer for the cells to elaborate melibiase, even the galactose was not utilized as a substrate. Preexposure to galactose seemed to be necessary for the cells to utilize galactose and melibiose. Galactose fermentation by GA cells was enhanced by the exposure of the cells to galactose, but not to melibiose, raffinose, sucrose or glucose. Delayed fermentation of sucrose by the cells exposed to glucose or melibiose, but not to galactose, was observed. Raffinose fermentation was obtained by the cells with either SU RAF or GA ME genes, but the enhanced fermentation of raffinose seemed to be dependent on which inducer the cells were exposed previously and enzymes induced by the inducer to break either one of the linkages of raffinose molecule, the alpha0galactosidic or the beta-fructo-furanosidic.

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Milk Concentration by Commerical Tubular Membranes (관형 상용막에 의한 우유 농축)

  • 김인철;김정학;탁태문
    • Membrane Journal
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    • v.9 no.1
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    • pp.17-24
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    • 1999
  • Milk was concentrated by commercial tubular membranes, The permeation rate reduction of hydrophilic membranes (sulfonated poly sulfone (SPSf), polyacrylonitrile (PAN), cellulose acetate (CA)) was found not to be large but hydrophobic membranes to be pronounced considerably, In the case of UF concentration total solids, proteins, fats and minerals were increased as concentrated but carbohydrates decreased. NF showed the same behavior except carbohydrates showing small reduction rate.

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Effects of Some Carbohydrates and Ammonium Sulfate on Lignin Degradation by Pseudomonas diminuta (탄수화물과 황산암모늄이 Pseudomonas diminuta의 리그닌 분해에 미치는 영향)

  • 김규중;신광수;맹진수;성치남
    • Korean Journal of Microbiology
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    • v.26 no.2
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    • pp.129-136
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    • 1988
  • To investigate the influence of cosubstrate supplement and ammonium sulfate on lignin degradation by Pseudomonas diminuta KM-4-2, isolated in the laboratory, the strain was cultured on the lignin media which contained lignin as a source of carbon and the culture filtrate was analyzed by Sephadex G-75 column chromatography. It was found that polymerization was not appeared unlike wood-rot fungi. When the carbohydrates were added, the peak of lignin at 280nm by UV scanning spectra of the filtrate, was significantly increased. In order to determine the effect of ammonium sulfate on the ligninolytic activity, the isolated strain was incubated in the media containing 0.1%, 0.25% and 0.5% of nitrogen concentration in the Warburg flask and the rate of oxygen uptake was esitmated by Warbuge Respirometer. As a result, the activity was maximum at 0.1% of nitrogen concentration and thereafter decreased in parallel with nitrogen concentration.

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The Removal Rate of the Constituents of the Litters in the Aquatic Plant Ecosystems I. Phragmites longivalvis Grasslands in a Delta of the Nakdong River (수생식물 생태계에 있어서 낙엽의 구성성분의 유실률 I. 낙동강 삼각주지역의 갈대 초지)

  • 장남기;오경환
    • Asian Journal of Turfgrass Science
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    • v.9 no.4
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    • pp.331-342
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    • 1995
  • An investigation was performed to reveal the removal rates of organic constituents of the litters in a Phragmithea longivalvis grassland in a Delta of the Nakdong River, The removal rates of the inorganic and organic materials are determined by the mathematical models. The removal rates and time required to decay up to a percentage of each organic constituent were calculated using these new models. The removal rates of cold water soluble fractions, other carbohydrates, hot water soluble fractions, cellulose, crude fat, lignin and crude protein were 2.67, 1.39, 1.25, 1.02, 0.92, 0.49 and 0.47, respectively, The periods required to reach half time to the steady state of the removal and accumulation for cold water soluble fractions, other carbohydrates, hot water soluble fractions, cellulose, crude fat, lignin and crude protein of the litter were 0.26, 0.50, 0.55, 0.68, 0.75, 1.41 and 1.48 years, respectively.

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