• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.3
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• pp.243-250
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• 1998

This study was conducted to evaluate mineral content of Winter Rye, Nitrogen Balance, Soil chemical characteristics and NO,-N level in infiltration water as affected by different slurry application on the basis of N contents respectively and was arranged as a randomized complete block design with seven treatments (chemical fertilizer 160kg Nha, cattle slurry 160 . 320 - 480kg Nha, swine slurry 160 - 320 - 480kg Nha) and conducted at National Livestock Research Institute, RDA, in Suweon fiom Sep. 1996. to Apr. 1997. The results obtained are summarized as follows : Nirogen, Phosphorous, Calcium and Potassium of Winter Rye slightly increased as slurry application level was increased (p$NO_3$-N content ($\mu$g/g) of Winter Rye was shown fiom 780 to 1,462 in all treatments, especially , the swine slurry plots were significantly higher than that of cattle sluny plots (p < 0.05). As slurry application increased, show a marked trend toward surplus N input in all treatment. The pH of the topsoil (0~IOcm) was shown 6om 5.1 to 5.4 except the plot of chemical fertilizer before experiment pH of 5.1, but the pH of the subsoil (10~20cm) tended not to be regular. The content of available ,TEX>$P_2O_5$ inaeased with the increased application of slurry, particularly, the topsoil (O- 1Ocm) were much higher than the subsoil (10~20cm). The $NO_3$-N content of infiltration water (depth:lm) was increased as affected by sluny application.

• Biomedical Science Letters
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• v.10 no.3
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• pp.195-202
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• 2004

Lung cancer is a leading cause of cancer death worldwide; however, despite major advances in cancer treatment during the past two decades, the prognostic outcome of lung cancer patients has improved only minimally. This is largely due to the inadequacy of the traditional screening approach of diagnosis in lung cancer, which detects only well­established overt cancers and fails to identify precursor lesions in premalignant conditions of the bronchial tree. In recent years this situation has fundamentally changed with the identification of molecular abnormalities characteristic of premalignant changes; these concern tumour suppressor genes, loss of heterozygosity at crucial sites and activation of oncogenes. Basic knowledge at the molecular level has extremely important clinical implications with regard to early diagnosis, risk assessment and prevention, and therapeutic targets. In this study we used a 'cap-finder' subtractive hybridization method, 'long distance' polymerase chain reaction (PCR), streptavidin magnetic beads mediated subtraction, and spin column chromatography to detect differential expression genes of human small cell lung carcinoma. We have now isolated ninety two genes that expressed differentially in the human small cell lung carcinoma cells and analyzed of 12 clones with sequencing, nine cDNAs include tapasin (NGS-17) mRNA, BC200 alpha scRNA, chromosome 12q24 PAC RPCI3-462E2, protein phosphatase 1 (PPPICA), translocation protein 1 (TLOC1), ribosomal protein S24 (RPS24) mRNA, protein phosphatase (PPEF2), cathepsin Z, MDM2 gene and three novel genes. They may be oncogenesis­related proteins.

• Journal of environmental and Sanitary engineering
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• v.5 no.2
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• pp.81-94
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• 1990

The view of this research was to investigate the state of a sanitary management in the industrial area of Masan and Changweon cities through the nutritionists who works in the group food service facilities. The following results are as indicated . 1. The management types of group food service facilities are a direct management 89.7% , a consignned management 7.7% , an(1 a semimanagement 2.6% . 2. The survey for purulent inflammation and a possible disease of a cook is 59.0%. In the case of a wound of hand and foot and disease, the rate of non-worked people is 51.3% 3.4 regular medical examination of a cook was generally well executed, but only 64.1% were received an examination of the feces, and 88.0%, among them had one time per 6months. 4. Wearing a cap, a gown, and a private foot-wear in a kitchen of the cook was well executed, but nowhere was found to put on a mask 5. Only 11.5% of the group feeding facilities had a their own sterilizing lamp, and the company with a repository in a low temperature was 25.6% , 6.4 private toilet of the cook were estabilshed at rate of 38.5% , and also a private toilet providing with a sterilizing soap was 52.6% . 7. The pasteurization of a kitchen table before cooking was 28.2%, In the case of using a cooking dipper, spoon, tweezers when one taste was 83.3% , and using a hand when one dish up was 74.4 %. 8. When one use a raw egg, washing a shell was 35.9%. 9. The rate of a nutritionists writing a diary of the sanitary check-up was 75.6%, and only 23.1% of the facilities was provided with a preserved food. 14.1% of the nutritionists did a sanitary eaucation periodically.

• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.281-293
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• 1992

Classification of the cycle of seminiferous epithelia into 12 stages by the morphological changes in acrosomal system and evaluation of the relative frequency of stages and the cell association were histologically performed in the mature Korean native Jin-do dogs. The results were summarized as follows; 1. The minimum number of type A spermatogonia averaged 1.01 at stages I, while maximum number averaged 2.47 at stages XII. Some type A spermatogonia divided at stage XII to produce the type intermediate(IN) spermatogonia at the following stage I. The type IN spermatogonia divided at stage IV to produce the type B spermatogonia at stage V. 2. The type B spermatogonia divided at stage VI to produce the preleptotene primary spermatocytes at stage VII. The secondary spermatocytes observed at stage XII. The secondary spermatocytes observed at stage XII divided to give rise to the round spermatids at the following stage I. The numbers of the first spermatocytes and spermatids were almost constant, respectively, through all the cycles of seminiferous epithelium. 3. The acrosomal vesicle was invaginated to occupy one third to half of spermatid nucleus at the cap phase, which was different from that of rodent and ruminant spermatid nuclei. 4. The relative frequencies of stages I to XII of seminiferous epithelia cycle were 10.34, 4.84, 5.03, 8.22, 10.86, 6.63, 6.42, 18.88, 10.17, 6.18, 7.62% and 4.81%, respectively.

• Journal of Korean Society on Water Environment
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• v.26 no.4
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• pp.598-607
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• 2010

One of constraints in the application of unit-load method to estimate non-point pollution loads in the total water pollutant load management system (TWPLMS) is the limited numbers of applicable unit-loads. Since only 7 unit-loads are currently available for total 28 land-use categories in the national land register data, each unit-loads inevitably have to represent several land-use categories regardless of their actual land coverage characteristics. As a way to minimize the problem, this study suggested a nested application of the available unit-loads based on the analysis of high resolution aerial images taken in the Kyeongan watershed. Statistical analysis of three selected land-use categories such as school, apartment complex, and golf course showed that there exit significant (95% confidence level) relationships between the registered land-uses and actual land coverages. The school and apartment complex currently considered as 100% ground have only 65% and 80% of ground characteristics, respectively. Golf course, which is considered as 100% pasture, has about 5% of ground area. This indicates that the unit-load method using in TWPLMS can give over estimated non-point pollutant loads for the school and apartment complex (19.8~54.4%) but under estimation for the golf course (80.9%).

• Journal of Korean Society on Water Environment
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• v.26 no.6
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• pp.903-909
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• 2010

The purpose of this study was evaluated on the applicability of Load Duration Curve Method (LDC Method) using HSPF watershed model and sampling data for efficient TMDLs in Korea. The LDC Method was used for assessment pollutant characteristics in watershed and water quality variation in each water flow level. Load Duration Curve is applied for judge the level of impaired water-body and can be estimated the impaired level by pollutant, such as BOD, T-N, and T-P in this study depending on variation of stream flow. As a result, BOD, T-P was usually exceed the standard value at low flow and dry hydrologic period. Improvement of effluent concentration from WWTP and riparian buffer protection zone are effective to improve the water quality. T-N showed the worst condition at mid-range hydrologic period and moist hydrologic period. Therefore, soil erosion control program and BMPs for non-point source pollution control is effective for recovery the water quality, which can be useful method for management of water quality in the plan of recovery water quality spontaneously. Applicability of LDC Method was evaluated in the Nakbon A watershed. However, we need to consider more detailed and accumulated data set such as accurate GIS data and detail pollution data, and WWTP discharge water quality data for accurate evaluation of watershed. Overall, The LDC Method is adequate for evaluation of watersheds characteristics, and its application is recommended for watershed management and TMDL Implementation.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.94-99
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• 2016

Since development of the first Korean button mushroom cultivar 'Sae-Ah' in 2010, mushroom farmers have demanded an improved cultivar. Although a new cultivar, 'Saejeong', was developed in 2011, this cultivar has thus far been difficult to cultivate in typical mushroom farms. To solve this problem, another new cultivar, 'Saedo', was developed in 2012. This new cultivar was selected by genetic analysis from crossing the heterokaryon line A175 and the homokaryon line ASI1346-15. The heterokaryon A175 was a crossed line between strains ASI1038-211 and ASI1346-20. The mycelium of 'Saedo' showed good growth on compost dextrose agar at $25^{\circ}C$. The morphological traits such as the pileus (cap) and stipe of the 'Saedo' cultivar were thicker than those of the reference control 'Saejeong', and the production yield was increased by 1.89-fold compared to that of the reference control. A field experiment of the 'Saedo' cultivar has been ongoing since 2013. The yield of the 'Saedo' cultivar was found to be substantially higher than that of the reference control during the $2^{nd}$ flush.

• Journal of the Korean Wood Science and Technology
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• v.38 no.2
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• pp.124-134
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• 2010

This study was performed to evaluate the efficiency of using softwood as the sawdust medium for Lentinula edodes cultivation, effect of nutrient on the mycelial growth, spawning, the mushroom yield, and quality. The nitrogen nutrition significantly enhanced the mycelial growth of L. edodes. The glutamic acid in the L. leptolepis and P. koraiensis, and asparagine in the P. densiflora were appeared to slight increase in the mycelial growth. The vegetable oil showed very effective on the mycelial growth in the P. koraiensis sawdust medium. Carbon/nitrogen ratio of all the test was reduced after mycelial growth. The mycelial growth was exclusively dependent on reduction of carbon. The mushroom yield (32.7%) of the P. densiflora sawdust medium (carbon source: 3% active carbon, nitrogen source: 0.4% asparagines) was the best in mushroom production of L. edodes, followed by the Q. variabilis sawdust (35.4%) of the control medium. The diameter of mushroom cap was obtained from the P. densiflora sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate) and P. koraiensis sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate), and the P. koraiensis sawdust (carbon source: 3% xylose, nitrogen source: 0.4% glutamic acid, supplement: 0.05% amino acid), with values 71.5 mm, 71.5 mm and 72.1 mm, respectively. In the polypropylene bag cultivation, the weight losses of the block medium gradually increased for 80 days in the dark (13.8~16.8%) and then became stable in the range of 20.7~25.8%.

• Restorative Dentistry and Endodontics
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• v.21 no.1
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• pp.242-253
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• 1996

Penetration degree of several dyes into dentinal tubules in vivo was compared with in vitro, and experimental sensitivity of those dyes was investigated in 64 canines of 16 cats. Dentin 1mm below cusp tip was exposed by cross-sectioning with diamond disc. Pulp of 32 canines were extirpated through cervical cavities. In the presence of smear layer or after add-etching with 37% phosphoric acid to the exposed dentin surface, acrylic collar and resin cap was attached to keep dyes. 52 mmol/$\ell$ Evans' blue, 2% Methylene blue, 10 % Silver nitrate and 5% Fluorescene were then applicated on each 4 canines respectively for 30 minutes. After rinsing, the canines were ground-sectioned longitudinally and linear dye penetration was measured under microscope. The results obtained were as follows ; 1. Evans' blue and Methylene blue penetrated significantly(P<0.05) more in vitro than in vivo only in the cases that exposed dentin surfaces were not etched with acid. 2. Silver nitrate penetrated significantly(P<0.05) more in vitro than in vivo in both cases that exposed dentin surfaces remained intact and were etched with acid. 3. The penetration degree of Fluorescene did not show statistical significance between in vivo and in vitro or in acid-etched and in not acid-etched cases.

• The Korean Journal of Malacology
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• v.26 no.4
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• pp.311-316
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• 2010

Spermatid differentiations during spermiogenesis and mature sperm ultrastructure in male Crassostrea nipponica were investigated by transmission electron microscope observations. The morphology of the spermatozoon of this species has a primitive type and is similar to those of other bivalves. Mature spermatozoa consist of broad, cap-shaped acrosomal vesicle and an axial rod in subacrosomal materials on an oval nucleus showing deeply invaginated anteriorly, two triplet substructure centrioles surrounded by four spherical mitochondria, and satelite fibres, which appear near the distal centriole. The acrosomal vesicle of spermatozoa of C. nipponica resemble to those of other investigated ostreids. Especially, two transverse bands (stripes) appear at the anterior region of the acrosomal vesicle, unlikely 2-3 transverse bands (stripes) in C. gigas. It is assumed that differences in this acrosomal substructure are associated with the inability of fertilization between the genus Crassostrea and other genus species in Ostreidae. Therefore, we can use sperm morphology in the resolution of taxonomic relationships within the Ostreidea. The sperm is approximately $48-50{\mu}m$ in length including an oval sperm nucleus (about $1.0{\mu}m$ in length and $1.41{\mu}m$ in width), an acrosome (about $0.48{\mu}m$ in length and 0.30 in width) and tail flagellum ($46-48{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9 + 2 structure. These morphological charateristics of acrosomal vesicle belong to the family Ostreidae in the subclass Pteriomorphia.