• 대한예방한의학회지
• /
• 제17권3호
• /
• pp.165-176
• /
• 2013

Objective : The purpose of this study was to establish the simultaneous analysis for six compounds in Gumiganghwal-tang (GMGHT, Jiuweiqianghuo-tang) and to investigate the anti-atherosclerotic effects of GMGHT in vitro. Methods : The column for separation of six compounds was used Luna $C_{18}$ column and maintained at $40^{\circ}C$. The mobile phase for gradient elution consisted of two solvent systems, 1.0% acetic acid in water and 1.0% acetic acid in acetonitrile. The analysis was carried out at a flow rate of 1.0 mL/min with pothodiode array (PDA) detection at 254, 280, and 320 nm. The injection volume was 10 ${\mu}L$. The antioxidant activities of GMGHT were evaluated by measuring free radical scavenging activities on 2,2'-Azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH). The inhibitory effects on low-density lipoprotein (LDL) oxidation were evaluated by the formation of thiobarbituric acid relative substances (TBARS), relative electrophoretic mobility (REM), and fragmentation of apolipoprotein B (ApoB)-100. Results : Calibration curves were acquired with $r^2{\geq}0.9998$. The contents of liquiritin, ferulic acid, baicalin, baicalein, glycyrrhizin, and wogonin in GMGHT were 1.784, 1.693, 37.899, 0.258, 1.869, and 0.034 mg/g, respectively. The GMGHT showed the radical scavenging activity in a dose-dependent manner. The concentration required for 50% reduction ($RC_{50}$) against ABTS and DPPH radicals were 72.51 ${\mu}g/mL$ and 128.49 ${\mu}g/mL$. Furthermore, GMGHT reduced the oxidation properties of LDL induced by $CuSO_4$. Conclusion : HPLC-PDA is considered as an available and convenient method for quality control and standardization of GMGH and GMGHT has potentials on anti-atherosclerosis by anti-oxidative effect and suppressive effect on LDL oxidation.

• Bulletin of the Korean Chemical Society
• /
• 제33권9호
• /
• pp.3055-3060
• /
• 2012

A simple, rapid and sensitive spectrofluorometric method was developed for the determination of folic acid (FA), based on its quenching effect on the fluorescence intensity of enoxacin (ENX)-europium ($Eu^{3+}$) complex as a fluorescent probe. Fluorometric interaction between ENX-$Eu^{3+}$ complex and FA was studied using UV-visible and fluorescence spectroscopy. The quenched fluorescence intensity at an emission wavelength of 614 nm was proportional to the concentration of FA. Optimum conditions for the determination of FA were investigated. Under optimal conditions, the reduced fluorescence intensity at 614 nm was responded linearly with the concentration of FA. The linearity was maintained in the range of $1.25{\times}10^{-9}$ to $1.50{\times}10^{-7}$ M (R = 0.9986) with the limit of detection ($3S_b/m$) (where $S_b$ is the standard deviation of blank and m is the slop of linear calibration curve) of $6.94{\times}10^{-10}$ M. The relative standard deviation (RSD) for 9 repeated measurements of $1.0{\times}10^{-9}$ M FA was 1.42%. This method was simple, cost effective, and relatively free of interference from coexisting substances. Successful determinations of FA in pharmaceutical formulation and biological samples with the developed method were demonstrated.

• Journal of Radiation Protection and Research
• /
• 제10권1호
• /
• pp.14-28
• /
• 1985

고순도 물질의 $K_{\alpha}$형광 X-선을 이용하여 100 keV 이하에서의 단색 Photon 선원을 개발하였다. 단색 Photon은 방사선 측정기기의 교정에 매우 유용한 것으로 얇은 금속박막의 Radiator에 제동복사선을 조사하여 얻을 수 있다. 본 실험에서는 $_{47}Ag,\;_{50}Sn,\;_{68}Er,\;_{70}Yb,\;_{82}Pb$, 등의 Radiator를 사용하여 20 keV 에서 80 keV의 범위에서 단색 Photon을 얻어내어 고순도 HpGe LEPS로 0.64-0.94에 이르는 단색도를 얻어내었고 600cc 얇은창이온전리함을 사용하여 8.3mR/h 에서 232. 5mR/h에 이르는 선량률을 얻어 내였다.

• 생약학회지
• /
• 제45권4호
• /
• pp.300-314
• /
• 2014

An ultra-performance liquid chromatography-electrospray ionization-mass spectrometer (UPLC-ESI-MS) method was established for the simultaneous quantification of eighteen marker compounds in traditional Korean formula, Cheonwangbosimdan (CWBSD). In addition, we evaluated the antioxidant effects of CWBSD. Eighteen marker components were separated on a UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) and kept at $45^{\circ}C$ by gradient elution with 0.1% (v/v) formic acid in water and acetonitrile as mobile phase. The flow rate was 0.3 mL/min and the injection volume was $2.0{\mu}L$. The antioxidant activities of CWBSD were assessed by measuring free radical scavenging activities on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH). The calibration curves of all analytes showed good linearity (correlation coefficient ${\geq}0.9937$) within the test ranges. The limits of detection and quantification for the 18 marker compounds were 0.01-4.71 ng/mL and 0.03-14.13 ng/mL, respectively. The contents of the 18 compounds in CWBSD extract ranged from none to $1701.00{\mu}g/g$. The CWBSD showed the radical scavenging activity in a dose-dependent manner. The concentration required for 50% reduction ($RC_{50}$) against ABTS and DPPH radicals were $149.42{\mu}g/mL$ and $339.24{\mu}g/mL$.

• 한국근적외분광분석학회:학술대회논문집
• /
• 한국근적외분광분석학회 2001년도 NIR-2001
• /
• pp.1158-1158
• /
• 2001

Fast and dynamic biochemical, enzymatic and morphological changes occur during the so-called generative development and during the vegetative processes in seeds. The most characteristic biochemical and compositional changes of this period are the formation and decline of storage components or their precursors, the change of their degree in polymerization and an extensive change in water content. The aim of the present study was to detect the maturation processes in seed nondestructively and to verify the applicability of near infrared spectroscopic methods in the measurement of physiological, chemical and biochemical changes in wheat seed. The amount and variation of different water “species” has been changed intensively during maturation. Characteristic changes of three water absorption bands (1920, 1420 and 1150 nm) during maturation were analysed. It was concluded that the free/bound transition of water molecules could be followed sensitively in different region of NIR spectra. Kinetic changes of carbohydrate reserves were characteristic during maturation. An intensive formation and decline of carbohydrate reserves were observed during early stage of maturation (0 -13 days, high energy demand). An accelerated formation of storage carbohydrates (starch) was detected in the second phase of maturation. Five characteristic absorption bands were analysed which were sensitive indicators the changes of carbohydrates occurred during maturation. Precursors of protein synthesis and the synthesis of reserve proteins and their kinetic changes during maturation were followed from NIR spectra qualitative and qualitatively. Dynamic formation of amino acids and the changes of N forms were detected by spectroscopic, chromatographic and by capillary electrophoresis methods. Calibration equations were developed and validated in order to measure the optimal maturation time protein and moisture content of developing wheat seeds. The spectroscopic methods are offering chance and measurement potential in order to detect fine details of physiological processes. The spectra have many hidden details, which can help to understand the biochemical background of processes.

• Journal of Biosystems Engineering
• /
• 제34권1호
• /
• pp.50-56
• /
• 2009

Surface plasmon resonance (SPR) biosensor has been used to detect many biochemical reactions, because this label-free sensor has high sensitivity and rapid response. The reactions are monitored by refractive index changes of the SPR biosensor. Iprovalicarb is protective, curative, and eradicative systemic fungicide introduced by Bayer AG in 1999. It has potential far control of downy mildew infesting onion, cucumber, grape and melon, late blight infesting tomato and potato, and anthracnose infesting watermelon and pepper. It is strictly limited to the maximum residue limit. In this study, the applicability of a portable SPR biosensor (Spreeta, Texas instrument, TX, USA) to detect the iprovalicarb residue was examined. The sensor chip was adopted to detect the reaction of iprovalicarb to immobilized iprovalicarb-antibody. The binding of the iprovalicarb onto the biosensor surface was measured by change of the refractive index (RI). Characteristics of the sensor chip including specificity, sensitivity, stability, and reusability were analyzed. In calibration test for seven levels of iprovalicarb concentration (0.32 to 5,000 mg/L) with three replications, a Sigmoidal model with Hill function was obtained between relative RI value and the iprovalicarb concentration with R-square of 0.998. It took 30 minutes to complete a set of detecting assay with the SPR biosensor.

• 대한교통학회지
• /
• 제26권5호
• /
• pp.73-80
• /
• 2008

ALINEA는 램프의 하류부에 설치된 검지기를 이용하여 최적의 차량점유율 상태를 유지하도록 유입램프의 교통량을 조절하는 방안으로 검지기를 이용한 차량의 점유율을 제어 변수로 이용하고 있다. 하지만, 현재 가장 널리 사용되고 있는 루프제어기 점유율의 정확도가 비교적 낮다는 점과 점유율은 검지기 길이의 함수로서 ALINEA의 적용 시 설치 지점마다, 그리고 검지기 길이마다 최적 점유율 보정과정이 필요한 점을 감안할 때 현재 사용 중인 ALINEA를 보완할 필요가 있다. 관리자와 이용자 측면에서 점유율이 사실상 인지하기 어려운 변수임을 감안할 때 쉽고 간편한 변수의 사용을 통한 모형개발이 의미를 가질 수 있을 것이다. 본 연구에서는 ALINEA 알고리즘의 기본 개념을 이용하되 제어변수인 점유율을 이용할 때의 불편한 점 및 단점을 일부 개선시킬 수 있는 속도 변수를 이용하여 ALINEA 모델을 보완하고자 한다.

• 한국측량학회지
• /
• 제25권5호
• /
• pp.427-436
• /
• 2007

각종 시설물들의 건설로 인해 형성된 사면은 붕괴의 위험성을 앉고 있기 때문에 사면의 현장에 대한 정보를 수집하기 위한 기존의 조사 방법들은 조사기간의 장기화, 계측장비에 대한 비용 및 접근성 등의 문제점이 제기되고 있으며, 이는 자료 수집의 한계점으로 제시되고 있다. 본 연구에서는 어느 위치에서든지 줌렌즈를 사용하여 영상을 취득할 수 있도록 하기 위하여 줌렌즈를 초점거리별로 검정하여 자유로이 줌을 사용할 수 있도록 하였다. 초점 거리별 검정 데이터 값은 DB(Date Base)화하여 여러 종류의 카메라나 또는 다른 렌즈를 사용하여 측정을 하더라도 자동으로 촬영한 초점거리의 카메라 검정데이터 값과 카메라정보를 불러오도록 Image Loader 시스템을 개발하였다. 또한, 취득한 대상물의 입체영상으로부터 3차원 공간영상정보를 구축함으로써 사면측량 및 조사에 효율적인 기초 자료로 제공하고자 하며, 사람이 접근할 수 없는 위험사면에 대한 정확한 측량방법을 제시하고자 한다.

• Journal of Electrochemical Science and Technology
• /
• 제8권1호
• /
• pp.43-52
• /
• 2017

In this paper, for the first time, the electroanalytical study of Triethylenediamine, TEDA was done on a typically graphene modified carbon paste electrode (Gr/CPE) in pH=10.5 of phosphate buffer solutions (PBS). The surface morphology of the bare and modified electrodes was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The electro-oxidation of TEDA was investigated at the surface of modified electrode. The results revealed that the oxidation peak current of TEDA at the surface of Gr/CPE is 2.70 times than that shown at bare-CPE. A linear calibration plot was observed in the range of 1.0 to 202.0 ppm. In this way, the detection limit was found to be 0.18 ppm. The method was then successfully applied to determination of TEDA in aqueous samples obtained from two kinds of activated carbon from the masks of war. On the other hand, density functional theory (DFT) method at B3LYP/6-311++G** level of theory and a conductor-like Polarizable Continuum Model (CPCM) was used to calculate the $pK_a$ values of TEDA. The energies of lowest unoccupied molecular orbital ($E_{LUMO}$) and highest occupied molecular orbital ($E_{HOMO}$), gap energy (${\Delta}E$) and some thermodynamic parameters such as Gibbs free energy of TEDA and its conjugate acid ($HT^+$) were calculated. The results of calculated $pK_a$ were found to be in good agreement with the experimental values.

• 한국가스학회지
• /
• 제11권4호
• /
• pp.54-58
• /
• 2007

본 연구에서는 13L급 전자제어식 디젤 엔진을 혼소 엔진으로 개조하여 얻은 결과를 다루고 있다. 혼소 엔진 개조 후 차량의 출력, 연료경제성, 운전성 등을 파악하기 위하여 일정한 경로의 고속도로를 주행하였다. 개조된 차량은 최대 85%의 대체율을 가지며 500 km의 거리를 혼소 모드로 주행하였다. 차량의 주행성능은 디젤 모드의 경우와 거의 동일하였으나, 추월 가속성능이 약간 떨어지는 경향이 있었다. 엔진의 최대출력, 열효율, 배기가스 측정을 위하여 차량 시험과는 별도로 엔진 시험도 동시에 수행하였다. ND-13모드에서 배출가스 배출량 측정 실험을 수행한 결과, 산화촉매를 장착한 혼소모드에서의 배출가스 배출량은 디젤모드에서와 출력은 동일하게 유지하면서 K2006 규제를 만족함을 알 수 있었다.