• Restorative Dentistry and Endodontics
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• v.27 no.5
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• pp.463-472
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• 2002

Bacterial lipopolysaccharide (LPS) plays a major role in stimulating the synthesis and release of the principal osteoclast-activating cytokines, namely, interleukin 1 and tumor necrosis factor-$\alpha$ from immune cells. Although rnonocytes/macrophages are the main producers of these cytokines, recent evidence has indicated that polymorphonuclear leukocytes (PMN) have the ability to release IL-1 and TNF-$\alpha$. Calcium hydroxide has been shown to be an effective medicament in root canal infections, reducing the microbial titre within the canal. It has been proposed that the therapeutic effect of Ca(OH)$_2$ may also be the result of direct inactivation of LPS. The purpose of this study was to investigate whether treatment of Porphyromonas endodontalis LPS with calcium hydroxide alters its biological action as measured by human PMN secretion of IL-1 and TNF-$\alpha$, and it was compared with Escherichia coli LPS. P. endodontalis ATCC 35406 was cultured in anaerobic condition, and LPS was extracted using the hot-phenol water extraction method and purified. Purchased E. coli LPS was also purified. 100 $\mu\textrm{g}$/ml of each LPS in pyrogen free water were incubated with 25mg/ml Ca(OH)$_2$ at 37$^{\circ}C$ for 7 days. The supernatants were subjected to ultrafiltration, and the isolates were lyophilized and weighed. PMNs were obtained from peripheral blood by centrifugation layered over Lymphoprep. The cells were resuspended (4$\times$10$^6$ cells/ml) in RPMI 1640 followed by treatment with various concentrations of LPS (0, 0.1, 1, 10$\mu\textrm{g}$/ml) for 24 hours at 37$^{\circ}C$ in 5% $CO_2$ incubator. The supernatants of cells were collected and the levels of IL-1$\alpha$, IL=1$\beta$ and TNF-$\alpha$ were measured by enzyme-linked immunosorbent assay. The results were as follows ; 1. The levels of IL-1$\alpha$, IL-1$\beta$, TNF-$\alpha$ from PMN treated with each LPS were significantly higher than those released from unstimulated PMN of the control group (p<0.05). 2. The levels of all three cytokines released from PMN stimulated with each calcium hydroxide treated LPS were significantly lower than those released from PMN stimulated with each untreated LPS (p<0.05), while they were not significantly different from those released from unstimulated PMN of the control group (p>0.05) 3. The levels of secretion for all three cytokines were affected in a dose-dependent manner in PMN stimulated with each LPS (p<0.05), but not in PMN stimulated with each calcium hydroxide treated LPS (p>0.05). 4. The levels of all three cytokines released from PMN stimulated with p. endodontalis LPS were significantly lower than those released from PMN stimulated with E coli LPS (p<0.05).

• Korean Journal of Soil Science and Fertilizer
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• v.14 no.4
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• pp.242-249
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• 1982

Effects of lime and ameliolating materials on decreasing available soil cadmium were studied, applying the amendments to Cd pre-and post-treated soils. Soil sttreated with Cd were placed in pots and kept under field moisture condition, summer through winter in 1981. The results of soil analysis made 40 to 60 days after the Cd treatment are as follows ; 1. Greater amount of Cd was extracted by 0.1N-HCl or 2% Citric acid than N-AcNH4 solution. More Cd was dissoluted by 0.1-HCl than 2% Citric acid. No Cd was extracted by pure water. Showing a wide variance in the amount of extractable Cd among treatments (amendments), the $N-AcNH_4$ solution seemed to be the most effective extracting solution of available soil cadmium. 2. Calcium hydroxide was the most effective materials in reducing $N-AcNH_4$ extractable Cd, followed by calcium carbonate and calcium silicate. 3. Superphosphate is also effective in reducing exchangeable cadmium. The reduction seemed to be attributed to the precipitation of cadmium phosphate. 4. The exchangeable cadmium by $N-AcNH_4$ was large in the soil pH range of 6.0 and 6.5, and it decreased as the soil pH became far apart from these values. The decrese of exchangeable Cd at low pH seemed to be related to the increase of $Mn^{+{+}}$ and that at the high pH to the precipitation as Cd-hydroxide.

• Polymer(Korea)
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• v.38 no.5
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• pp.656-663
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• 2014

Atorvastatin calcium-loaded polyoxalate (POX) microspheres were prepared by an emulsion solvent-evaporation/ extraction method of oil-in-oil-in-water ($O_1/O_2/W$) for sustained release. We investigated the release behavior according to initial drug ratio, molecular weight ($M_w$) and concentration of POX and concentration of emulsifier. The microsphere was characterized on the surface, the cross-section morphology and the behavior of atorvastatin calcium release for 10 days by scanning electron microscopy (SEM) and high performance liquid chromatography (HPLC). The analysis of crystallization was analyzed to use X-ray diffraction (XRD), differential scanning calorimeter (DSC) and Fourier transform infrared (FTIR). These results showed that the release behaviors can be controlled by preparation conditions.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.19 no.3
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• pp.265-286
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• 1997

Bone morphogenetic proteins(BMPs) are a group of transforming growth factor beta(TGF-${\beta}$)-related factors and multifunctional proteins, especially the only known biologic factors capable of inducing endochondral bone formation at an extraskeletal site. This study was performed to investigate the effect of the partially purified porcine BMP(pBMP) at an ectopic site. PBMP was partially purified from porcine bone matrix and its activity was monitored by an in vivo bioassay. The purification method utilized extraction of the bone-inducing activity with 4M guanidine, followed by chromatography on heparin-Sepharose. Active fractions were assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. And the fractions were reconstituted with inactive insoluble collagenous bone matrix from rats, acid soluble type I collagen from rat tail and chondroitin-6-sulfate sodium salt and implanted into the pectroralis muscle pouches of Sprague-Dawley rats. And the carrier complex was implanted on the opposite side as control. The rats were sacrificed at the day of 1st, 3rd, 5th, 7th, 11th, 14th and 21st after implantation and examined histologically, radiologically and biochemically. And alkaline phosphatase activity and calcium content were used as indices of bone formation. The results were as follows ; 1. Active fractions were localized in a zone between 31 and 40 KDa on SDS-PAGE. 2. The implanted 3.0mg of the partially purified pBMP induced cartilage and bone in the muscle tissue of rats through an endochondral ossification process. 3. Inactive insoluble bone matrix, type I collagen and chondroitin-6-sulfate have functioned as carriers for pBMP, but revealed some foreign body reactions. 4. Soft X-ray didn't reveal significant change between the experimental and the control group. 5. The alkaline phosphatase activities in the experimental group of 5th, 7th, 11th, 14th and 21st were increased significantly compared with control (p<0.01) with the peak in the group of 11th day. 6. With time, the calcium content of the experimental group increased. And the calcium contents in the experimental group of 11th, 14th and 21st were increased significantly compared with control (p<0.01).

• Resources Recycling
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• v.28 no.3
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• pp.35-44
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• 2019

In this study, we conducted experiments to store $CO_2$ and produce $CaCO_3$ through indirect carbonation using paper sludge ash (PSA) and three chelating reagents (fumarate, IDA and EDTA). Fumarate and IDA used as solvents could facilitate the indirect carbonation reaction to store more $CO_2$ than water. When 0.1 M fumarate and IDA were used, $CO_2$ storage was 63 and $89kg-CO_2/ton-PSA$, respectively, and $CaCO_3$ yield was 144 and $202kg-CaCO_3/ton-PSA$. For the case of EDTA, however, the carbonation was hardly progressed. As either the concentration or Ca-ligand stabilization constant of each chelating reagent increased, the calcium extraction efficiency from PSA increased. In addition, the carbonation efficiency was influenced by the Ca-ligand stabilization constant. As the Ca-ligand stabilization constant increased, more calcium could be extracted from the PSA. With the constant larger than that of $CaCO_3$ ($10^{8.35}$), however, the carbonation reaction was not proceeded.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.259-259
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• 2004

Porcine sperm extract (PSE) supporting Ca/sup 2+/ osillation was microinjected into the in vitro matured porcine oocytes. In the presence of the capacitative Ca/sup 2+/ entry mechanism which can activate MII oocytes, preparation methods of sperm extraction were studied by many researchers. Such as freeze-thaw cycle, homogenation, sonication of boar sperm was used for certification of their activity of calcium signals. (omitted)

• Journal of Sericultural and Entomological Science
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• v.40 no.1
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• pp.78-85
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• 1998

Colorants were prepared by extraction of natural indigo which was harvested just in the blooming season(in the late of July). 100 g of fresh leaves soaking in 1 ιwater was kept at 3$0^{\circ}C$, 30 hours. A solution of 3g/l calcium hydroxide was added into it to precipitate dye substance and it was freezing-dried into powder form. The fermentation and dyeing conditions were investigated. The results obtained are summarized as follows; K/S value of dyed silk fabrics of fermentation conditions was higher at 95$^{\circ}C$ for 20 min. than at 4$0^{\circ}C$ for 20 hours. Furthermore, K/S value of dyed silk fabric was raised by the addition of 5g/l of glucose and 5g/l of NaOH. K/S value of dyed silk fabric was raised by the addition of 5g/l of glucose and 5g/l of NaOH. K/S value increased as extending of dyeing time when dyed till 2 hours at 3$0^{\circ}C$. K/S value decreased in order of 3$0^{\circ}C$, 4$0^{\circ}C$ and 5$0^{\circ}C$, at the various dyeing temperatures and dyeing concentrations, and colour fastness ranged from 4 to 5 grade in terms of washing, perspiration and light fastness.

• Economic and Environmental Geology
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• v.29 no.1
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• pp.57-63
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• 1996

The Dalsung copper-tungsten mine in the Taegu area, Korea was closed in 1975 and may be the sources of the heavy metal contamination in the tributary system and soil-plant system due to the mine drainage derived from the mine wastes and tailings. To examine the degree and extent of heavy metal contamination in the vicinity of Dalsung mine area, stream water and soil samples were taken and analyzed for heavy metals by ICP-AES and AAS. Highly contaminated soils are found near the Lower Tunnel No.0 ranging up to $1760{\mu}g/g$ As, $2060{\mu}g/g$ Cu, $1120{\mu}g/g$ Pb and 346 ${\mu}g/g$ Zn. From the results of the sequential extraction methods for the metal speciation, the heavy metals in soils may be derived from soil parent materials and acid mine drainage. With the processes for the heavy metal removal, most of the heavy metal ions in the acid mine drainage are removed by being exchanged with Ca ions held by the bentonite, hydroxyapatite and calcium hydoxide.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.21 no.1
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• pp.81-88
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• 1999

A fibrin adhesive have been widely used in oral and maxillofacial surgery for microvascular anastomosis, autogenous chip bone grafts, many kinds of soft tissue surgery (vestibuloplasty, bleeding control after extraction, primary healing by covering of suture of a gum after the extirpation of large cysts). There are two principal components in adhesive systems biologically: lyophilized human fibrinogen and bovine thrombin. The fibrinogen component contains coagulation factor XIII and enhance the initial wound healing, which polymerizes soluble fibrin monomers into an insoluble clot. The thrombin is dissolved in a solution of calcium chloride to provide the second component. We applied fibrin adhesive, Beriplast (Behring, Behringwerke AG, D-3350, Marburg, FRD), to 4 patients for fixation of free skin grafting donors who had facial scar around eye, nose, mouth corner which received from accidents, or burn. We have experienced initial accelerated graft fixation between donor and recipient sites with no additional fixation. And It's made easy bleeding control and easy manipulation during operation. But two cases showed partial hypertrophic scar engrowth in above 3 months follow up, but no significant. Histopathological reviews in general were showed similar scar findings such as abundant collagen bundles in H&E, M/T stain, but slight positive signs in elastic and collagen antibody immunopathologic findings in hypertrophic scar cases.

• Microbiology and Biotechnology Letters
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• v.47 no.2
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• pp.303-309
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• 2019

Earlier studies by our group revealed that gallic acid in phytochemicals stimulated biofilm production in epiphytes, while caffeic acid in phytochemicals inhibited biofilm production in non-epiphytes. It is well documented that antimicrobial secretion by some epiphytic bacteria inhibits non-epiphytic bacterial growth on leaf surfaces. These selection criteria help plants choose their microbial inhabitants. Calcium and iron in phytochemicals also stimulate biofilm formation and thus, may be selection criteria adopted by plants with respect to their native epiphytic population. Furthermore, the processing of leaves during phytochemical extraction impacts the composition of the extract, and therefore its ability to affect bacterial biofilm formation. Computation of the Hurst exponent using biofilm thickness data obtained from the Ellipsometry of Brewster Angle Microscopic (BAM) images is an efficient tool for understanding the impact of phytochemicals on epiphytic and non-epiphytic populations when compared to fluorescent microscopy, scanning electron microscopy, and staining techniques. To the best of our knowledge, this is the first report that uses the Hurst exponent to elucidate the mechanism involved in plant microbe interaction.