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Porcine lymphotropic herpesvirus (Gammaherpesvirinae) DNA in free-living wild boars (Sus scrofa Linnaeus, 1758) in Brazil

  • Porto, Gisele S.;Leme, Raquel A.;Agnol, Alais M. Dall;de Souza, Tatiana C.G.D.;Alfieri, Amauri A.;Alfieri, Alice F.
    • Journal of Veterinary Science
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    • v.22 no.6
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    • pp.81.1-81.9
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    • 2021
  • Background: Suid gammaherpesvirus 3, 4, and 5 (porcine lymphotropic herpesvirus - PLHV-1, -2, and -3) are viruses that infect domestic and feral pigs. Objectives: This study examined the presence of PLHV DNA in biological samples from free-living wild boars circulating in a Brazilian geographical region with a high density of commercial domestic pigs. Methods: Lung samples of 50 free-living wild boars were collected by exotic wildlife controller agents between 2017 and 2019 in the state of Paraná, southern Brazil. Lung and spleen fragments were obtained from six fetuses collected by hysterectomy post mortem from a pregnant sow. A polymerase chain reaction (PCR) assay using consensus primers (pan-herpesviruses) was performed to detect PLHV DNA. The samples showing positive results for PLHV DNA were submitted to single-round PCR assays with the specific primers for identifying PLHV-1 (213-S/215-As), PLHV-2 (208-S/212-As), and PLHV-3 (886s/886As). The specificity of the species-specific PCR products was assessed by nucleotide sequencing of the amplicons. Results: Forty-eight (96%) of the 50 lung samples analyzed were positive for PLHV by PCR using pan-herpesvirus primers. In 33 (68.75%) of the positive samples, at least two PLHV species were identified simultaneously. The DNA of PLHV-1, -2, and -3 was found in free-living wild boars of all ages, but not in the fetuses, even though they were from a sow that tested positive for all three viruses. Conclusion: These viruses are endemic to the population of feral pigs in the Brazilian region evaluated, as well as in domesticated pigs.

Antimicrobial resistance and virulence factors in staphylococci isolated from canine otitis externa (개의 외이도에서 분리한 포도상구균의 항생제 내성 및 병독성 유전자)

  • Cho, Jae-Keun;Lee, Jung-Woo;Kim, Joung-Ok;Kim, Jeong-Mi
    • Korean Journal of Veterinary Service
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    • v.45 no.3
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    • pp.171-180
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    • 2022
  • The aim of this study was to investigate the prevalence of antimicrobial resistance and virulence factors in staphylococci isolated from canine otitis externa. A total 295 causative microorganisms were isolated. The most common isolated species were Staphylococcus (S) pseudintermedius (94 isolates) followed by Pseudomonas aeruginosa (60 isolates), S. schleiferi (25 isolates), Escherichia coli (23 isolates) and Proteus mirabilis (20 isolates). Staphylococci isolates were showed high resistance to penicillin (78.6%), erythromycin (55.9%), tetracycline (52.4%), clindamycin (51.7%) and ciprofloxacin (42.8%). Of the 145 staphylococci isolates, 49 (33.8%) methicillin-resistant staphylococci (MRS) were observed, distributed among S. pseudintermedius (n=34), S. schleiferi (n=6), S. epidermis (n=4), S. hominis (n=2), S. aureus, S. caprae and S. saprophyticus (n=1, respectively). Forty-three (87.8%) of 49 MRS and 10 (10.4%) of 96 methicillin-susceptibility staphylococci harbored mecA gene. About 80% of MRS were multidrug-resistant with resistance to at least one antibiotic in three or more antibiotic classes. Resistance genes blaZ (93/114, 81.5%), ermB (35/81, 43.2%), ermC (3/81, 3.7%), aacA-aphD (50/54, 92.5%), tetM (69/76, 90.7%) and tetK (6/76, 7.8%) were detected among resistant isolates. Virulence factors genes lukF and lukS were found in 100%(145/145) and 43.4%(63/145), respectively. Genes encoding ermA, eta, etb and tsst were not detected. To the best of our knowledge, this is the first study which investigated for the presence of genes encoding antimicrobial resistance and staphylococcal toxins in staphylococci isolated from canine otitis externa. A continuous monitoring and surveillance program to prevent antimicrobial resistance in companion animals is demanded.

Development of Air Spring Damper System(ASDS) (공기 스프링 댐퍼 시스템(ASDS)의 개발)

  • Kim, Dong Baek;Park, Heung Sik
    • Journal of the Society of Disaster Information
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    • v.17 no.4
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    • pp.829-838
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    • 2021
  • Purpose: The Air Spring Damper System (ASDS) is proposed when existing concrete structures that have not been seismic resistant for economic and technical reasons or low-rise concrete structures that are difficult to earthquake. Method: To conduct a study on the damping force antigen in the kinetic equation of free vibration, we analyze whether this device has damping ability as a damper experimentally and theoretically, and examine the possibility of field application. Result: The air damper system is considered to be more economical than steel hysteresis dampers even if the number of dampers increases due to its easy manufacture and construction and low restrictions on shape, size, material, etc. Conclusion: In an air spring damper system, it is essential to reduce the diameter of the air inlet/outlet hole to improve the damping ratio, and in this case, if the diameter exceeds a certain lower limit, consideration of the compressibility of air is required, so further research is needed.

Standard Model for Mobile Forensic Image Development

  • Sojung, Oh;Eunjin, Kim;Eunji, Lee;Yeongseong, Kim;Gibum, Kim
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.2
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    • pp.626-643
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    • 2023
  • As mobile forensics has emerged as an essential technique, the demand for technology development, education and training is increasing, wherein images are used. Academic societies in South Korea and national institutions in the US and the UK are leading the Mobile Forensic Image development. However, compared with disks, images developed in a mobile environment are few cases and have less active research, causing a waste of time, money, and manpower. Mobile Forensic Images are also difficult to trust owing to insufficient verification processes. Additionally, in South Korea, there are legal issues involving the Telecommunications Business Act and the Act on the Protection and Use of Location Information. Therefore, in this study, we requested a review of a standard model for the development of Mobile Forensic Image from experts and designed an 11-step development model. The steps of the model are as follows: a. setting of design directions, b. scenario design, c. selection of analysis techniques, d. review of legal issues, e. creation of virtual information, f. configuring system settings, g. performing imaging as per scenarios, h. Developing a checklist, i. internal verification, j. external verification, and k. confirmation of validity. Finally, we identified the differences between the mobile and disk environments and discussed the institutional efforts of South Korea. This study will also provide a guideline for the development of professional quality verification and proficiency tests as well as technology and talent-nurturing tools. We propose a method that can be used as a guide to secure pan-national trust in forensic examiners and tools. We expect this study to strengthen the mobile forensics capabilities of forensic examiners and researchers. This research will be used for the verification and evaluation of individuals and institutions, contributing to national security, eventually.

Strength and toughness prediction of slurry infiltrated fibrous concrete using multilinear regression

  • Shelorkar, Ajay P.;Jadhao, Pradip D.
    • Advances in concrete construction
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    • v.13 no.2
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    • pp.123-132
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    • 2022
  • This paper aims to adapt Multilinear regression (MLR) to predict the strength and toughness of SIFCON containing various pozzolanic materials. Slurry Infiltrated Fibrous Concrete (SIFCON) is one of the most common terms used in concrete manufacturing, known for its benefits such as high ductility, toughness and high ultimate strength. Assessment of compressive strength (CS.), flexural strength (F.S.), splitting tensile strength (STS), dynamic elasticity modulus (DME) and impact energy (I.E.) using the experimental approach is too costly. It is time-consuming, and a slight error can lead to a repeat of the test and, to solve this, alternative methods are used to predict the strength and toughness properties of SIFCON. In the present study, the experimentally investigated SIFCON data about various mix proportions are used to predict the strength and toughness properties using regression analysis-multilinear regression (MLR) models. The input parameters used in regression models are cement, fibre, fly ash, Metakaolin, fine aggregate, blast furnace slag, bottom ash, water-cement ratio, and the strength and toughness properties of SIFCON at 28 days is the output parameter. The models are developed and validated using data obtained from the experimental investigation. The investigations were done on 36 SIFCON mixes, and specimens were cast and tested after 28 days of curing. The MLR model yields correlation between predicted and actual values of the compressive strength (C.S.), flexural strength, splitting tensile strength, dynamic modulus of elasticity and impact energy. R-squared values for the relationship between observed and predicted compressive strength are 0.9548, flexural strength 0.9058, split tensile strength 0.9047, dynamic modulus of elasticity 0.8611 for impact energy 0.8366. This examination shows that the MLR model can predict the strength and toughness properties of SIFCON.

Leisure Activities Experienced by Old Women in Farm (농촌여성노인의 여가체험)

  • Kang, Hyun Lim
    • 한국노년학
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    • v.30 no.4
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    • pp.1377-1392
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    • 2010
  • The purpose of this study was to understand the meaning and essence of leisure activities experienced by older females in rural communities and, in reference to that, to provide basic data to help develop health-promoting nursing intervention programs by investigating the indications of leisure and its effects on older individuals' life process. The qualitative research method of van Manen's Hermeneutical analysis and Phenomenological research are useful to study the true meaning and nature of experiences inherited from their language, belief, culture and social traditions. Consequently, the leisure that the women from the rural community were found to have experienced came down to a total of seven themes or activities: (a) to overcome loneliness through social interaction and get-togethers; (b) to achieve balance between body and mind by engaging in physical activities; (c) to try and help ease the burden on one's children; (d) to simply kill time; (e) to find oneself small pleasures; (f) to learn something new and feel proud; and (g) to live one's life as maintaining communication with the outside world. The study herein confirmed that the female elderly in rural area spend their free time on appreciating the nature, maintaining health and social interaction and hanging around with the peers of similar age to overcome economic woes and the feeling of estrangement unique in the social and economic context of rural communities.

The Net Generation Debate: Unpacking Individual Perceptions and Lived Experiences toward Technology Use in Education

  • CHOI, Hyungshin;SO, Hyo-Jeong
    • Educational Technology International
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    • v.13 no.2
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    • pp.257-281
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    • 2012
  • This study aims to examine individual perceptions and lived experiences of the Net generation of student teachers and the previous generation of teachers about their technology use in education. The participants of this study include 106 pre-service teachers and 50 in-service teachers from one teacher education university in Korea. Employing a mixed methodology, we first empirically examined the participants' perceptions toward multiple variables related to technology use in education, namely (a) past ICT experiences in schools, (b) personal computer use, (c) constructivist belief, (d) computer efficacy, (e) attitude toward computer in education, and (f) prospective computer use. In addition, we conducted face-to-face interviews with selected participants for the in-depth investigation of their lived experiences about technology use, beliefs, and attitude. Results indicate that there are significant differences between in-service and pre-service teachers in their prior experiences with technology in schools. However, the pre-service and in-service teachers did not differ significantly in their beliefs, attitude and other technology-related variables, which may indicate the danger of generational determinism in the Net Generation debate. The analysis of interview narratives revealed two major themes about the interplay of one's agency and structural changes in the participants' lived experiences with technology use in education: (a) transition from negative past experiences to opportunities for positive computer use, and (b) attitudes formation and change through apprenticeship experiences and structural influences. In conclusion, this study suggests that the Net Generation debate should move beyond dichotomous or techno-centric thinking. There is a critical need to pay more attention to develop deeper understandings of the fundamental diversity existing within the generation itself. Implications for teacher education are also discussed.

Using DQ method for vibration analysis of a laminated trapezoidal structure with functionally graded faces and damaged core

  • Vanessa Valverde;Patrik Viktor;Sherzod Abdullaev;Nasrin Bohlooli
    • Steel and Composite Structures
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    • v.51 no.1
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    • pp.73-91
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    • 2024
  • This paper has focused on presenting vibration analysis of trapezoidal sandwich plates with a damaged core and FG wavy CNT-reinforced face sheets. A damage model is introduced to provide an analytical description of an irreversible rheological process that causes the decay of the mechanical properties, in terms of engineering constants. An isotropic damage is considered for the core of the sandwich structure. The classical theory concerning the mechanical efficiency of a matrix embedding finite length fibers has been modified by introducing the tube-to-tube random contact, which explicitly accounts for the progressive reduction of the tubes' effective aspect ratio as the filler content increases. The First-order shear deformation theory of plate is utilized to establish governing partial differential equations and boundary conditions for the trapezoidal plate. The governing equations together with related boundary conditions are discretized using a mapping-generalized differential quadrature (GDQ) method in spatial domain. Then natural frequencies of the trapezoidal sandwich plates are obtained using GDQ method. Validity of the current study is evaluated by comparing its numerical results with those available in the literature. After demonstrating the convergence and accuracy of the method, different parametric studies for laminated trapezoidal structure including carbon nanotubes waviness (0≤w≤1), CNT aspect ratio (0≤AR≤4000), face sheet to core thickness ratio (0.1 ≤ ${\frac{h_f}{h_c}}$ ≤ 0.5), trapezoidal side angles (30° ≤ α, β ≤ 90°) and damaged parameter (0 ≤ D < 1) are carried out. It is explicated that the damaged core and weight fraction, carbon nanotubes (CNTs) waviness and CNT aspect ratio can significantly affect the vibrational behavior of the sandwich structure. Results show that by increasing the values of waviness index (w), normalized natural frequency of the structure decreases, and the straight CNT (w=0) gives the highest frequency. For an overall comprehension on vibration of laminated trapezoidal plates, some selected vibration mode shapes were graphically represented in this study.

Dye-Perfused Human Placenta for Simulation in a Microsurgery Laboratory for Plastic Surgeons

  • Laura C. Zambrano-Jerez;Karen D. Diaz-Santamaria;Maria A. Rodriguez-Santos;Diego F. Alarcon-Ariza;Genny L. Melendez-Florez;Monica A. Ramirez-Blanco
    • Archives of Plastic Surgery
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    • v.50 no.6
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    • pp.627-634
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    • 2023
  • In recent decades, a number of simulation models for microsurgical training have been published. The human placenta has received extensive validation in microneurosurgery and is a useful instrument to facilitate learning in microvascular repair techniques as an alternative to using live animals. This study uses a straightforward, step-by-step procedure for instructing the creation of simulators with dynamic flow to characterize the placental vascular tree and assess its relevance for plastic surgery departments. Measurements of the placental vasculature and morphological characterization of 18 placentas were made. After the model was used in a basic microsurgery training laboratory session, a survey was given to nine plastic surgery residents, two microsurgeons, and one hand surgeon. In all divisions, venous diameters were larger than arterial diameters, with minimum diameters of 0.8 and 0.6 mm, respectively. The majority of the participants considered that the model faithfully reproduces a real microsurgical scenario; the consistency of the vessels and their dissection are similar in in vivo tissue. Furthermore, all the participants considered that this model could improve their surgical technique and would propose it for microsurgical training. As some of the model's disadvantages, an abundantly thick adventitia, a thin tunica media, and higher adherence to the underlying tissue were identified. The color-perfused placenta is an excellent tool for microsurgical training in plastic surgery. It can faithfully reproduce a microsurgical scenario, offering an abundance of vasculature with varying sizes similar to tissue in vivo, enhancing technical proficiency, and lowering patient error.

Production of Saccharogenic Mixed Grain Beverages with Various Strains and Comparison of Common Ingredients (다양한 균주를 이용한 혼합곡물 발효음료의 제조와 일반 성분 비교)

  • Lee, Jae Sung;Kang, Yun Hwan;Kim, Kyoung Kon;Lim, Jun Gu;Kim, Tae Woo;Kim, Dae Jung;Bae, Moo Hoan;Choe, Myeon
    • Journal of the East Asian Society of Dietary Life
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    • v.24 no.1
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    • pp.53-61
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    • 2014
  • In this study, we investigated the production process and the chemical composition of saccharogenic mixed grain beverages (SMGBs). Various SMGBs were prepared through saccharification with Aspergillus (A.) oryzae CF1003 (A), A. acidus KACC46420 (B), Rhizopus (R.) delemar KACC46149 (C), R. oryzae KACC45714 (D), R. oryzae KACC46148 (E), A~E mixed strains (F), A. oryzae CF1001 (G), A. acidus CF1005 (H) and A+H mixed strains (I)-starter at $53^{\circ}C$ for 24 hours. The saccharogenic power of the strains was higher in samples F and G. The soluble solid ($^{\circ}Brix$) of SMGBs were the highest in Sample C. The moisture, crude protein, crude lipid, and crude ash content of various SMGBs showed a range of 77.9~80.7%, 3.7~7.5%, 0.37~0.97% and 1.81~7.47%, respectively. The viscosity of various SMGBs were in the range of 60~528. Further, free amino acid contents of SMGBs were in the range of 347~1,352 ${\mu}g/$ 100 g, respectively. From these results, we could secure the possibility and basic information for the development of SMGBs products. For future studies, we need to improve the taste and functionality of the products.