• 농업과학연구
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• 제51권2호
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• pp.169-178
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• 2024

Cordyceps militaris L. (C. militaris) has been traditionally used as tonic medicine for metabolic syndrome. Cordycepin, has been reported with immunomodulatory, antitumor, and hepatoprotective effect, is the main extract from C. militaris. This study was conducted to evaluate the alcohol degradation and hepatoprotective effect of cordycepin-enriched C. militaris extract (CM) powder in chronic and binge ethanol (ethanol Lieber-DeCarli diet)-fed male C57BL/6 Mice. Cordycepin-enriched C. militaris extract powder was orally administered once daily at dose levels of 0, 125, 250, and 500 mg·kg^-1·day^-1 for 16 days. For evaluating alcohol degradation, ethanol concentration and alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity were measured in serum. Serum ethanol (EtOH) concentration was decreased at CM treated groups, and the activities of ADH and ALDH were increased dose-dependently at CM treated groups compare to EtOH model group. In clinical chemistry, the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were lower in CM treated groups than those in EtOH model group. Additionally, absolute and relative (to body weight) liver weights were statistically decreased in the CM treated groups compared to the EtOH model group. In conclusion, our study showed that cordycepin-enriched C. militaris extract powder exhibits hepatoprotective effect by upregulating the ADH and ALDH enzyme in an alcoholic liver disease model.

• 열처리공학회지
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• 제31권1호
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• pp.6-11
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• 2018

The anodic oxidation behavior of Si-containing aluminum alloy for diecasting was investigated. Especially, the property changes during anodization both on aluminum 1050 and 9 weight percentage silicon containing aluminum (Al-9Si) alloys were analyzed by the static current test. In order to fabricate a uniform anodic oxidation film by effect of Al-Si compound, nitric acid containing hydrofluoric acid had been used as a desmutter for aluminum alloy after alkaline etching. It was found that the level of voltage of Al-9Si alloy during the static current test was almost as double as higher than aluminum 1050 through anodization. By adding hydrofluoric acid in the nitric acid electrolyte, the silicon compound on the surface was removed, and the optimum amount of added hydrofluoric acid could be derived. It was also observed that the size of silicon compound formed on the surface could be refined by heat treatment at $500^{\circ}C$ and followed water quenching.

• 대한수의학회지
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• 제35권3호
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• pp.583-593
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• 1995

The present study was conducted to develop a toxoplasma latex agglutination test antigen(Test-MT) and evaluate the toxoplasma latex agglutination(LA) test using a newly-made "Test-MT kit" by comparing with the Toxo-MT kit(Eiken chemical co, Tokyo). Also, the specifity and sensitivity test were made by comparing with IFA test and IgG-ELISA. Tachyzoite suspensions of Toxoplasma gondii(RH strain) were ultracentrifuged for 30min at $60,000{\times}g(4^{\circ}C)$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $1.0{\mu}m$ in diameter(Polyscience co) were used for the preparation of sensitized latex-antigen supension(Test-MT). The frequency distribution of LA titers in Test-MT showed two peaks at <1:32 and 1:128. The borderline titer for positive test in Test-MT was determined to be 1:64. But the frequency distribution of LA tites in Toxo-MT showed two peaks at <1:16 and 1:64. The positive borderline was determined to be 1:32. Agreement of reactions between Test-MT and Toxo-MT kit by LA test was shown 92.5% in bovine sera and 97.0% in swine sera, respectively. From the results obtained here it was determined that the sensitized latex-antigen, Test-MT kit, for the microtiter agglutination test prepared as same as by the procedure described in the previous paper(Suh and Lee, 1993) was useful as a highly specific, sensitive and stable immunotiteration reagent for serodiagnosis of toxoplasma infection in animal sera.

• 한국축산식품학회지
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• 제30권3호
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• pp.403-409
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• 2010

Accurate methods for the identification of closely related species or breeds in raw and processed meats must be developed in order to protect both consumers and producers from mislabeling and fraud. This paper describes the development of DNA markers for the discrimination and improvement of Korean native pig (KNP) meat. The KIT gene is related to pig coat color and is often used as a candidate marker. A 538 bp fragment comprising intron 19 of the pig KIT gene was amplified by PCR using specific primers, after which the PCR amplicons of a number of meat samples from KNP and three major improved breeds (Landrace, Duroc and Yorkshire) were sequenced in order to find a nucleotide region suitable for PCR-RFLP analysis. Sequence data showed the presence of two nucleotide substitutions, g.276G>A and g.295A>C, between KNP and the improved pig breeds. Digestion of KIT amplicons with AccII enzyme generated characteristic PCR-RFLP profiles that allowed discrimination between meats from KNP and improved pig. KNP showed three visible DNA bands of 264/249, 199, and 75 bp, whereas DNA bands of 249, 199, and 90 bp were detected in the three improved pig breeds. Therefore, the 75 bp DNA fragment was specific only to KNP, whereas the 90 bp DNA fragment was specific to the improved breeds. The breed-specific DNA markers reported here that target the KIT gene could be useful for the identification of KNP meat from improved pig meats, thus contributing to the prevention of falsified breed labeling.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 1996년도 추계 학술대회 및 정기총회
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• pp.65-66
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• 1996

• 대한기관식도과학회지
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• 제9권1호
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• pp.87-91
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• 2003

Currently gastrointestinal mesenchymal tumors are divided into three major categories: myogenic tumors(leiomyoma, leiomyosarcoma), neurogenic tumors (schwannomas) and neoplasms that belong to neither group, which are known by GIST(gastrointestinal stromal tumors). The stromal tumors are hetrogenous, so that they may show myogenic or neurogenic differentiation or both, or no differentiation at all in some patients. The best defining feature for GIST is their expression of KIT-protein(CD117). Leiomyomas are the most common mesenchymal tumor in esophagus. Esophageal GISTS are very rare in comparision to those of the stomach and intestine. Recently we experieneced one case of the esophageal GIST, so that we describe an esophageal GIST on immunohistochemical analysis. A 70 years old woman complained of dysphagia and nausea for 3 days. FGS showed a huge elevated lesion in lower esophagus 33cm distal to incisor, which was covered with normal mucosa. CT and UGI showed the intramural tumor of lower third of the esophagus. The distal esophagectomy and esophago-gastrostomy were performed. The tumor was located in lower third of esophagus and measured as $6{\times}3.7$cm in size. Immunohistochemically, it showed weakly positive CD117 and diffusely positive S-100. SMA, desmin, NES and chromogranin showed negative immune-reaction. The patient was followed for 15 month after operation. There was no recurrence.

• Korean Chemical Engineering Research
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• 제56권2호
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• pp.261-268
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• 2018

본 연구에서는 수열합성법과 주형합성법을 이용하여 메조포어를 지닌 $TiO_2$를 합성하였다. 수열합성법을 이용해서 anatase 구조의 메조포러스 $TiO_2$를 합성했다. Rutile 구조의 메조포러스 $TiO_2$를 제조하기 위해서 수열합성법으로 제조된 메조포러스 $TiO_2$를 $300^{\circ}C$부터 $700^{\circ}C$까지 소성시켰더니 $600^{\circ}C$부터 anatase에서 rutile 결정구조로 상전이가 일어났다. 하지만, 메조포어가 붕괴되었다. 메조포어을 지닌 $TiO_2$를 합성하기 위해서 메조포러스 실리카 KIT-6을 주형으로 사용하는 주형합성법을 사용하였다. 먼저 메조포어 내부에 $TiO_2$를 형성시키고 소성온도를 800, $900^{\circ}C$로 높여서 anatase에서 rutile로의 상전이 거동을 조사하였다. 수열합성을 통해 제조된 자유로운 상태의 메조포러스 $TiO_2$의 경우 $600^{\circ}C$에서 anatase에서 rutile로의 상전이가 일어났지만 제한된 공간인 메조포러스 기공 내부에 형성된 $TiO_2$의 경우 $800^{\circ}C$까지 가열하더라도 rutile구조로 상전이가 일어나지 않았고, $900^{\circ}C$로 소성시키자 일부의 anatase가 rutile로의 상전이가 일어나기 시작하였다. 이러한 상전이는 산소 빈자리의 형성에 의해서 일어나야 한다고 알려져 있지만 실리카 기공 내부에 형성된 $TiO_2$는 실리카 기공 표면이 산소 빈자리 형성을 방해해서 상전이가 억제되는 것으로 판단된다. $900^{\circ}C$의 높은 소성온도로 인해서 anatase와 rutile 구조가 섞여있으며 실리카 기공 내부에 형성된 $TiO_2$는 NaOH 수용액을 이용해서 주형인 KIT-6과 분리해서 메조포어를 지닌 $TiO_2$를 제조하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.264-265
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• 2000

Helicobacter pylori(H. pylori) is the causative agent of chronic gastritis and the single most important factor in peptic ulcer disease, however, the pathogenetic mechanisms underlying H, pylori infection are not well understood. Futhermore, there is a strong association between H. pylori infection and gastric cancer. Various diagnostic methods for detecting H. pylori infection are available. These can be divided into invasive methods, requiring endoscopy, and non-invasive tests, mainly 13C-urea breath tests and serologic detection of antibodies. Rapid urease test is the most recommendable endoscopic test for the diagnosis of H. pylori infection, presently. CLO test kit is the represent of rapid urease test kits. The principles of CLO test kit is that hydrolysis of urea by urease Is detected by a dye indicators showing a color change. Our device is used same principle but we improved the reaction time is more faster and positive color change is more distinctive from the color of the negative specimen. So, this kit is more reliable because it response faster and accuracy.

• 대한치과보철학회지
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• 제32권2호
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• pp.212-224
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• 1994

The purpose of this study was to evaluate and compare film thickness of five kinds of resin luting cements [Comspan, Panavia Ex, Maryland bridge adhesive, All-bond C & B cementation kit, and Super-bond C & B]. Zinc-phosphate cement and glass-ionomer cement were used as the control group. In order to measure the film thickness the methods used were in broad compliance with ADA Specification No. 8, a tapered-die system that simulates clinical conditions more closely, and the connected tapered-die system that simulates bridge conditions. The inorganic filler size of resin cements was also examined with scanning electron micrographs. The results were obtained as follows ; 1. The film thickness of resin cements was increased in the order of Comspan, Panavia Ex, Super-bond C & B, Maryland bridge adhesive, and All-bond C & B cementation kit. Maryland bridge adhesive and All-bond C & B cementation kit showed significantly higher film thickness than the control group(p<0.01). 2. For all resin cements, there was a significant difference of film thickness between the ADA method and the tapered-die system. Generally, the tapered-die system demonstrated lower film thickness than the ADA method(p<0.01). 3. There was no significant difference in film thickness between the tapered-die system and the tapered-die bridge system in all resin cements(p<0.01). 4. The scanning electron microscope showed that the cement with larger filler had a tendency to be higher in film thickness.

• 디지털콘텐츠학회 논문지
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• 제14권3호
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• pp.381-390
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• 2013

휴대폰 사용자들 대부분이 스마트폰으로 옮겨가면서 사용자들은 이제 스마트폰으로 다양한 게임을 즐기고 있다. 그런데 현재 앱스토어에 기기의 성능을 고려한 2D 게임은 다양하게 올라오고 있지만 3D 게임의 수는 아직도 많이 부족한 것이 실정이다. 이는 3D 게임이 많은 컴퓨팅 성능을 필요로 하기 때문인데, 본 논문에서는 안드로이드에서 NDK를 이용하여 3D큐브 게임을 구현하였다. 기존 비주얼C++ 일리히트 3D 큐브 소스를 NDK를 이용해 안드로이드 시스템 라이브러리로 이식하고 편의 기능을 추가하는 방식으로 구현하였다. NDK를 이용했으므로 기존 C++ 코드의 대부분이 자바 코드로 변환되지 않고 그대로 실행될 수 있어서 실행 시험 결과 비교적 구형 스마트폰 상에서도 3D 그래픽 동작들이 무난하게 이루어짐을 확인할 수 있었다.