• Journal of the East Asian Society of Dietary Life
• /
• v.9 no.2
• /
• pp.207-213
• /
• 1999

This study was carried out to get a industrial information about a possibility of IgY antibody production, antimicrobial activity and properties of IgY antibody in egg yolk. The residual antibody activities of IgY were 91.5% after heating for 30min at 6$0^{\circ}C$. At the same time. these activities, were 73.2% after heating 15min at $65^{\circ}C$ and decreased vapidly at 7$0^{\circ}C$ and little antibody activity was left after heating for 15min at 8$0^{\circ}C$. When the prepared IgY was incubated at various pH ranges from 7 to 2 for 5hr at 37$^{\circ}C$., the antibody activity was stable from pH 7 to 4 and remained to 69.8% at pH 3.

• Parasites, Hosts and Diseases
• /
• v.56 no.1
• /
• pp.61-70
• /
• 2018

We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results. Sensitivity and specificity of Zika RDT in a field test was determined using positive and negative samples from Brazil and Korea. The diagnostic accuracy of Zika RDT was fairly high; sensitivity and specificity for IgG was 99.0 and 99.3%, respectively, while for IgM it was 96.7 and 98.7%, respectively. Cross reaction with dengue virus was evaluated using anti-Dengue Mixed Titer Performance Panel (PVD201), in which the Zika RDT showed cross-reactions with DENV in 16.7% and 5.6% in IgG and IgM, respectively. Cross reactions were not observed with West Nile, yellow fever, and hepatitis C virus infected sera. Zika RDT kit is very simple to use, rapid to assay, and very sensitive, and highly specific. Therefore, it would serve as a choice of method for point-of-care diagnosis and large scale surveys of ZIKV infection under clinical or field conditions worldwide in endemic areas.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.18 no.3
• /
• pp.33-40
• /
• 2019

FDM 3D printers have become widespread, and investment in the 3D printer industry is increasing. Therefore, many 3D printers are released and the functions of products are emphasized. However, to lower unit prices, open-type 3D printers are sold in kit form, and their performance is very low. If the 3D printer has many heat sources and is sealed, there is the possibility that the main accessories (the main board, power supply, and motor) will be damaged by trapped heat. At the same time, if the ambient temperature is low due to the properties of the material, the output quality deteriorates. In this study, we analyzed the temperature rise of the main accessories and the quality of the output by the heat bed when a chamber was added to an open-type 3D printer. We also compared the quality of the output due to the air flow with the temperature rise of the main accessories. Moreover, we found the optimal value. As a result of the quality analysis, it was finally confirmed that the case with the chamber at $95^{\circ}C$ was the best for the printing condition. In addition, in the absence of the chamber, the bending of the specimen was found to be large, and in the case of the chamber, the degree of bending was slightly decreased by 0.05 mm.

• Journal of Yeungnam Medical Science
• /
• v.15 no.1
• /
• pp.143-150
• /
• 1998

The aim of this study was to evaluate domestic enzyme immunoassay(EIA) kit 'LG RCD 3.0' (LG) for the detection of antibody to hepatitis C virus(anti-HCV) in comparision with Axsym HCV version 3.0(Axsym), Cobas Core anti-HCV EIA(Cobas). Cobas kit shows better clear distinction between positive and negative by signal/cutoff ratio(S/C), but it also reveal relatively high false positive rate. The concordance rate of test results between LG and Axsym was 96.2%, between LG and Cobas was 95.5%, and total agreement between three EIA kit was 93.9%. LG were relative poor distinction between positive and negative results, but it could be applied clinically as a screening tool for hepatitis C in general population. The SIC of one false negative result by LG was 0.91, and false positive were less than 4.0, therefore we concluded it is necessary to confirm by immunoblotting assay when SIC were between 0.8 and 4.0.

• Korean Journal of Agricultural Science
• /
• v.39 no.3
• /
• pp.421-425
• /
• 2012

The objective of this study was to improve the detection limit of rapid detection kit for Salmonella Typhimurium by image analysis system. The rapid detection kit was comprised of four elements: sample pad, conjugate pad, nitrocellulose pad and absorbent pad. Gold nanoparticle and Salmonella antibody were used as a tag and a receptor. Salmonella antibody and goat rabbit IgG antibody were used as test and control lines on nitrocellulose membrane. The color intensity of test line began to increase from $10^5CFU/mL$ of Salmonella sample. A multiple linear regression analysis was employed to explain the relationship between predicted and measured number of Salmonella cells. The developed model could successfully predict the cell number of Salmonella with validation against extra-experimental result.

• Journal of the Korea Society of Computer and Information
• /
• v.19 no.10
• /
• pp.91-97
• /
• 2014

In this paper, we design and implement a mobile game based on windows phone platform. Visual Studio and the Windows Mobile software development kit(SDK) and developer tool kits (DTK) make it possible to create software for the Windows Mobile platform in Visual C#. Also The XNA Framework is the set of .NET libraries that developers will code against to build games. XNA Framework support PC and XBOX and a ZUNE HD multiplex platform environment and 2D and 3D games provides the necessary functions to a development. XNA Framework is to make it easier to develop games because the codes which are succeeded creates with automatic, implements a game development relation resource and the game logic. The key idea of our game is that we use shooting and ranking system to raise fun.

• Korean Journal of Agricultural Science
• /
• v.51 no.2
• /
• pp.159-167
• /
• 2024

Herbal medicinal mushroom Cordyceps militaris has been traditionally used as tonic medicine for metabolic syndrome. Cordycepin, main extract of C. militaris, has been reported with immunomodulatory, anticancer, and hepatoprotective effects. This study was conducted to evaluate the potential hepatoprotective effect of cordycepin-enriched Cordyceps militaris, against high fat diet (HFD)-induced hepatic steatosis (HS) in male obese (ob/ob) mice. HFD was provided to ob/ob mice ad libitum (except negative control). Cordycepin-enriched C. militaris extract powder (CM) was orally administered once daily at dose levels of 0, 125, 250, and 500 mg·kg^-1 for 4 weeks. During the study, body weight gain was statistically increased in all HFD fed groups compared to negative control, but body weight gain in CM 500 mg·kg^-1 treated group shows a low tendency compared to HS model group. In organ weights, absolute and relative weights (to body weight) in liver and perirenal adipose tissue were increased in all HFD treated groups except CM 500 mg·kg^-1 treated group compared to the negative control. In clinical chemistry, serum glucose and total cholesterol levels in CM 250 and/or 500 mg·kg^-1 treated groups were lower than HS model group. In microscopical examination, hepatocyte vacuolation with macrovesicles in HS model group was increased compared to negative control, but this finding was decreased in CM 500 mg·kg^-1 treated group compared to HS model group. In this study, CM exhibited hepatoprotective effects against hepatic steatosis at mg·kg^-1 in ob/ob mice.

• The Optical Journal
• /
• s.119
• /
• pp.52-55
• /
• 2009

이제 감시카메라는 우리 주변 곳곳에 설치되지 않은 곳을 찾아보기 힘들 정도로 광범위해 졌다. 아니 우리 눈이 미치는 곳보다 더 많은 곳에 설치되었다고 해도 과언이 아닐 정도로 보편화되었다. 그러나 아직까지 CCTV의 힘을 빌리지 못한 사각지대가 엄연히 존재하며 많은 범죄가 발생하고 있다. 그러기에 이제는 카메라의 대수만을 늘리기 보다는 지능적으로 판단하고 추적할 수 있는, 생각하는 감시카메라의 필요성이 더욱 증대되고 있는 시점이다.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.18 no.1
• /
• pp.158-162
• /
• 2014

Purpose: Reference material (RM) is defined as material that is safe and homogeneous enough about specified characteristic that is made with a purpose of using test of measurement or nominal characteristic. Certified reference material (CRM), which is issued by authorized organization, is defined as reference material that provides characteristic value, link uncertainty and retroactivity. The purpose of this paper was to evaluate recovery of radioimmunoassay by Certified Reference Material enclosed with a certificate and therefore to enhance reliability of test. Materials and Methods: WHO certified reference material is purchased from NIBSC (National Institute for Biological Standard and Control, United Kingdom) and made of 3 levels that are C-1 (low concentration), C-2 (medium concentration) and C-3 (high concentration) and measured for kit at the Seoul National University Hospital. Recovery rate is evaluated after measurement at four different days. Results: Recovery rate results using WHO certified reference material are T4 90%, Ferritin 88%, PSA 94%, Prolactin 99%, AFP 94% and TSH 93%. Conclusion: A procedure that appropriate accuracy, precision, specificity, sensitivity, reproducibility, and validate on the subject of kit for radioimmunoassay is essential. Recovery rate assay as extraction efficiency of analysis process is percent about already measuring results of analysis result after all measuring process. This is very important assessment standards of performance evaluation of immunoassay kit. Recovery rate results of 6 type used WHO CRM are satisfactory to 88~99%. This demonstrates that the radioimmunoassay is a very accurate measurement, which is very effectively utilized in clinical practice.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2011.04a
• /
• pp.394-397
• /
• 2011

애플의 아이폰4 스마트폰에 OpenCV 라이브러리를 활용하여 실시간 처리가 가능한 영상처리 프로그램을 구현하였다. 구현된 영상처리 프로그램은 이치화, 적응이치화, 에지 추출, 외곽선 추출 등이며 Objective-C 언어로 버튼 등의 인터페이스를 사용하는 아이폰 앱으로 구현되었다. 또한 증강현실을 구현하기 위해 Wagner가 개발한 ARToolKitPlus 라이브러리의 각 단계를 분석하였으며, 이 라이브러리를 이용해 Loulier가 아이폰에 구현한 VRToolKit을 이용하여 가상물체를 실시간으로 증강하는 실험을 진행하였다. 가상물체는 OpenGL ES 함수를 사용해 텍스처맵핑 기법으로 제작한 지구 모형으로 실시간 증강시에도 매끄럽게 정합되는 결과를 얻었다. 본 연구는 향후 스마트폰에서의 얼굴인식 등의 영상처리 프로그램 개발 및 멀티마커의 인식 등의 증강현실 프로그램 개발의 기본 토대로 사용될 수 있다.