• BMB Reports
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• v.32 no.1
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• pp.92-97
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• 1999

Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.

• Proceedings of the Microbiological Society of Korea Conference
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• 1998.04a
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• pp.48.2-48.2
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• 1998

• Journal of the Korean earth science society
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• v.45 no.1
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• pp.85-109
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• 2024

Detrital zircon U-Pb dating was performed to determine the depositional age and provenance of sediments in the Cretaceous Muju Basin in Muju-gun, Jeollabuk-do. Six samples were collected from the Seolcheon Tuff (SCT), Bangyiri Formation (BYR), Gobang Member of the Gilwangri Formation (GWR-G), Seochang Member of the Gilwangri Formation (GWR-S), Bukchang Member of the Gilwangri Formation (GWR-B), and Jeogsangsan Formation (JSS). Based on the dating results, the sedimentary strata of the Muju Basin were deposited for approximately 105.6-90.4 Ma corresponding to the Albian to Turonian. The youngest single zircon ages of about 94.4 Ma and 89.6 Ma were confirmed in the samples from the Bangyiri Formation and the Gobang Member of the Gilwangri Formation, respectively, distributed in the western part of the Muju Basin. The relative and numerical ages previously estimated based on the lithostratigraphic correlation of the Gilwangri conglomerate need to be revisited in further research. The results also suggest that most of the sediments filling the Muju Basin were supplied from a limited area adjacent to the basin.