• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2005년도 춘계 학술발표회
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• pp.156-156
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• 2005

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2003년도 추계 학술발표회
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• pp.144-144
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• 2003

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2005년도 제48회 추계 학술연구 발표회
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• pp.44-44
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• 2005

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2005년도 제48회 추계 학술연구 발표회
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• pp.67-67
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• 2005

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2005년도 제48회 추계 학술연구 발표회
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• pp.69-69
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• 2005

• International Journal of Industrial Entomology and Biomaterials
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• 제24권1호
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• pp.29-35
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• 2012

Bumblebees are widely used to pollinate various crops, especially tomato, in greenhouses and fields. Here, we investigated whether different sugar solutions have any effects on the oviposition and colony development of Bombus terrestris queens. The types of sugar solution used were a white sugar solution, brown sugar solution, dark brown sugar solution, and prepared sugar solution; all solution contained with a 40% sugar concentration and 0.3% sorbic acid. Among these sugar solutions, B. terrestris queens reared on the white sugar solution exhibited the best results; the rates of oviposition, colony foundation and progeny-queen production were $75.0{\pm}11.0$, $33.0{\pm}9.6$ and $21.7{\pm}7.3$, respectively, which corresponded to 1.1-33.0-fold increases over the results of other sugar solutions. Queens reared on the prepared sugar solution did not found any colonies. The death rate within one month was lowest for the queens reared on the white sugar solution at $26.1{\pm}10.9%$, which was 1.2-2.0-fold lower than the rate for the other sugar solutions. Furthermore, the numbers of progeny produced by queens reared on the white sugar solution, $131.4{\pm}38.8$ workers and $51.0{\pm}40.6$ queens, were also higher, corresponding to 1.1-1.2-fold increases compared to queens raised on the other sugar solutions. Therefore, the white sugar solution was the favorable sugar solution for the egg-laying and colony developmental characteristics of B. terrestris queens.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권1호
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• pp.43-48
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• 2005

We investigated possible effect of different concentration of sugar solution and addition of antiseptic in the solution on oviposition and colony development of Bombus ignitus and B. terrestris. The rates of oviposition, colony foundation and progeny-queen production of B. ignitus were 1.2-3.0 fold higher in the 40$\%$ sugar solution than those of the 50$\%$ sugar solution. The rates of oviposition, colony foundation and progeny­queen production were 1.1-2.6 fold higher in the 40$\%$ sugar solution added in 0.3$\%$ sorbic acid as antiseptic than those of the 40$\%$ sugar solution. Further, the death rate within one month was 1.7 fold lower in the 40$\%$ sugar solution added in 0.3$\%$ sorbic acid than that of 40$\%$ sugar solution alone. In the comparison of the colony development tested using imported sugar solution, the Beehappy???, the 40$\%$ sugar solution added to antiseptic and the 40$\%$ sugar solution with­out antiseptic, the 40$\%$ sugar solution added to antiseptic was about equal to the Beehappy??? in colony development of B. terrestris. Further, the number of adults produced was 1.2-3.0 fold higher in the 40$\%$ sugar solution added to antiseptic than that of the Bee­happy???. Therefore the 40$\%$ sugar solution was more effective than the 50$\%$ sugar solution, and the 40$\%$ sugar solution added to antiseptic was the most effective in colony development and mass rearing of bumblebee.

• International Journal of Industrial Entomology and Biomaterials
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• 제27권1호
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• pp.142-158
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• 2013

The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is widely utilized for greenhouse pollination in South Korea. Understanding the magnitude of genetic diversity and geographic relationships is of fundamental importance for long term preservation and utilization. As a first step, we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to the "DNA barcode" region and the complete internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA from 88 individuals collected in nine South Korean localities. The complete ITS2 sequences were longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated 112-bp long repeats. The 658-bp long mtDNA sequences provided only six haplotypes with a maximum sequence divergence of 0.61% (4 bp), whereas the ITS sequences provided 84 sequence types with a maximum sequence divergence of 1.02% (21 sites). The combination of the current COI data with those of published data suggest that the B. ignitus in South Korea and China are genetically a large group, but those in Japan can be roughly separated into another group. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population were found to exist among the South Korean populations of B. ignitus, which suggests panmixia. This finding is consistent with our understanding of the dispersal capability of the species.

• International Journal of Industrial Entomology and Biomaterials
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• 제20권1호
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• pp.19-24
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• 2010

Bumblebees are widely used to pollinate crops in greenhouses and fields. Here, we investigated whether different wake-up treatments during a short period of 1~3 days just before indoor rearing has any effects on oviposition and colony development of $CO_2$-treated Bombus ignitus queens and artificially hibernated B. terrestris queens The wake-up regimes were defined as 16L for 1 day (16L-1), 16 L per day for 3 days (16L-3), 24L for 1 day (24L-1), or 24D for 1 day (24D-1). Among these wake-up treatments, the oviposition rate and preoviposition period of B. ignitus queens reared at 24L-1 were 16.7~25.1% higher and 1.0~3.5 days shorter than other wake-up treatments. B. terrestris queens reared at 24L-1 also showed the best results for egg-laying characteristics, which were 8.9~18.8% higher for oviposition and 0.6~3.5 days shorter for preovipostion period than other wake-up treatments. Furthermore, B. terrestris queens reared at 24L-1 were 17.5% and 13.8% higher in rate of colony foundation and queen production, respectively, than other wake-up treatments. These results show that the most favorable wake-up treatment just before rearing for egg-laying and colony developmental characteristics of B. ignitus and B. terrestris queens was 24L-1. Overall, our findings indicate that a wake-up treatment just before rearing was effective for colony initiation and colony development of bumblebee queens.

• International Journal of Industrial Entomology and Biomaterials
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• 제16권1호
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• pp.7-13
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• 2008

We investigated mating conditions about care temperature of queen before mating, mating period, and number of queen per mating cage to improve mating rate of Bombus terrestris. Among $19^{\circ}C$, $22^{\circ}C$ and $25^{\circ}C$, care temperatures of queen before mating, queen cared at $19^{\circ}C$ was more effective than those at $22^{\circ}C$ and $25^{\circ}C$ in death rate during care and mating periods, and colony development In case of mating period, oviposition rate and preoviposition periods at queen mated during 3 days were 89.3% and 11.4 days, respectively, which was higher and earlier than those of queen mated during 5 days and 7 days. The rate of worker emergence, colony foundation and progeny-queen production at 3 days-mated queen were also 2.0-11.6% higher than those at queen mated during 5 days and 7 days. In number of queen per mating cage, the rate of worker emergence, colony foundation and progeny-queen production queen mated at mating cage with 10 queens and 30 males were 41.5%, 25.9% and 23.2%, respectively. These values correspond to 1.5-6.8 folds those queen mated at cage with 20 queens and 30 queens. Therefore, we supposed that care temperature favorable for B. terrestris queen was $19^{\circ}C$ and the period need to mating was 3 days, and the number of queen per mating cage ($55{\times}45{\times}65\;cm$) was 10.