• 한국유기농업학회지
• /
• 제20권2호
• /
• pp.243-258
• /
• 2012

본 시험은 유기농업 농가에서 이용하는 수정벌 방사와 일반 농가에서 이용하는 식물생장조절제 처리에 대한 기초연구로서 수정벌 방사 및 식물생장조절제 처리가 가지의 수량 및 품질에 미치는 영향을 구명하고자 실시한 시험의 결과는 다음과 같다. 가지의 수량은 수정벌 방사와 Tomatotone을 개화당일 화방살포한 처리구에서 수량이 가장 높았고, Tomatotone과 $GA_3$을 엽면살포한 것이, Tomatotone 처리와 화방살포한 것이 좋았다. 상품과율은 수정벌 방사와 Tomatotone을 개화당일 화방살포한 처리구에서 가장 높았으며, 수량과 마찬가지로 Tomatotone과 $GA_3$ 처리는 엽면살포한 처리구에서, Tomatotone 처리는 화방살포한 처리구에서 대체적으로 높았다. 잎내의 무기성분 중 전질소함량은 Tomatotone을 6일 간격으로 엽면살포한 처리구에서, 인산은 Tomatotone을 개화당일 화방살포한 처리구에서, 칼륨은 Tomatotone과 $GA_3$을 6일 간격으로 엽면살포한 처리구에서, 칼슘은 Tomatotone과 $GA_3$을 개화 2~3일전에 화방살포한 처리구에서, 마그네슘은 수정벌 방사와 Tomatotone을 개화당일 화방살포한 처리구에서, 회분은 Tomatotone과 $GA_3$을 3일 간격으로 엽면살포한 처리구에서 가장 많았다. 줄기내의 무기성분 중 전질소함량은 Tomatotone을 개화당일에 화방살포한 처리구에서, 인산, 칼륨 및 마그네슘은 Tomatotone을 3일 간격으로 엽면살포한 처리구에서, 칼슘은 Tomatotone을 개화 2~3일전에 화방살포한 처리구에서, 회분은 Tomatotone을 개화당일에 화방살포한 처리구와 3일 간격으로 엽면살포한 처리구에서 많았다. 과실내의 무기성분 중 전질소함량은 Tomatotone과 $GA_3$을 6일 간격으로 엽면살포한 처리구에서, 인산은 Tomatotone을 3일 간격으로 엽면살포한 처리구에서, 칼륨과 회분은 Tomatotone과 $GA_3$을 개화당일에 화방살포한 처리구에서, 칼슘과 마그네슘은 Tomatotone과 $GA_3$을 개화 2~3일전에 화방살포한 처리구에서 가장 많았다. 이상의 연구결과를 종합하면 유기농업 농가에서 이용하는 수정벌 방사가 식물생장조절제 처리를 대용할 만큼 효과가 인정되었으며, 가지의 착과와 상품과의 수량을 증대시키기 위해서는 수정벌 방사와 Tomatotone을 혼용해서 사용하면 수분노력이 절감되고 수량도 식물생장조절제를 처리한 것보다 높았으나 재배시기 및 작형에 따른 연구가 뒤따라야 할 것으로 생각된다.

• 한국응용곤충학회:학술대회논문집
• /
• 한국응용곤충학회 2005년도 춘계 학술발표회
• /
• pp.46-46
• /
• 2005

• International Journal of Industrial Entomology and Biomaterials
• /
• 제24권1호
• /
• pp.37-40
• /
• 2012

The apidaecins are highly active against Gram-negative bacteria. Here, we show the expression and antibacterial activity of the bumblebee, Bombus ignitus, apidaecin. We PCR-amplified 51 bp of the active domain sequence of the B. ignitus apidaecin gene and expressed the recombinant B. ignitus apidaecin active domain in baculovirus-infected insect cells. The recombinant B. ignitus apidaecin active domain shows bactericidal activity against Gram-negative bacteria, including Pseudomonas tolaasii, a serious pathogen in cultivated mushrooms, but not Gram-positive bacteria. This result suggests that the active domain of the B. ignitus apidaecin is a potential antibacterial agent for the control of bacterial brown blotch diseases.

• 한국잠사학회:학술대회논문집
• /
• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
• /
• pp.65-65
• /
• 2003

Artificial hibernation is essential for year-round rearing of the bumblebee, Bombus ignitus that undergoes one generation per year It is known that keeping the queens in low temperature for two or three months is effective to terminate their diapause and develop the colony. Temperature, time and surroundings to keep the queens during artificial hibernation were investigated. (omitted)

• 한국응용곤충학회:학술대회논문집
• /
• 한국응용곤충학회 2000년도 합동 춘계 학술발표회
• /
• pp.49-49
• /
• 2000

• International Journal of Industrial Entomology and Biomaterials
• /
• 제26권2호
• /
• pp.124-130
• /
• 2013

Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of $-2.5^{\circ}C$, $0^{\circ}C$, $2.5^{\circ}C$ and $5^{\circ}C$ with constant humidity >80%, the queens stored at $2.5^{\circ}C$ exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at $0^{\circ}C$, $5^{\circ}C$, $7.5^{\circ}C$ and $12.5^{\circ}C$. At $2.5^{\circ}C$ the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at $5^{\circ}C$. Thus, $2.5^{\circ}C$ and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.

• 한국응용곤충학회지
• /
• 제49권1호
• /
• pp.49-55
• /
• 2010

작물의 화분매개를 위해 사용되고 있는 뒤영벌 종 토종 호박벌에 적합한 전기를 이용한 산란유도장치를 본 연구에서 처음으로 개발하였다. 개발된 산란유도장치는 여왕벌의 산란이 이루어지는 산란상자, 산란상자의 온도 유지를 위한 온도제어장치, 열전도부 및 이를 지탱할 수 있는 이동형 선반 등으로 구성되어 있다. 개발된 산란유도장치와 기존의 온수 보일러 장치를 이용하여 토종 호박벌의 산란성과 봉세발달을 조사한 결과, 개발된 산란유도장치에서 산란율이 3.9% 높고, 봉군형성율도 5.2% 높은 것으로 확인되었다. 특히 개발된 산란유도장치는 기존의 장치보다 설치비용을 75% 줄일 수 있었고, 에너지 절감이 가능하여 유지비용에 있어서도 효과적이었다. 이러한 결과들로 볼 때, 본 연구에서 새롭게 개발된 전기 산란유도장치는 비용절감과 함께 효과적인 산란유도에 따른 호박벌의 대량생산에 기여할 수 있을 것으로 판단된다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제18권1호
• /
• pp.41-48
• /
• 2009

The large bumblebee, Bombus terrestris, indigenous to Europe and used extensively for high-value crop pollination, has been artificially introduced in several parts of the world. Here we show the interspecific hybridization between bumblebee species, B. terrestris and B. ignitus, under laboratory conditions. The mating and oviposition percentages of the interspecific hybridization of a B. terrestris queen with a B. ignitus male were higher than those of the intraspecific mating of B. ignitus. Furthermore, the competitive copulation experiment indicated that the mating of B. ignitus males with B. terrestris queens was 1.8-fold more frequent than with B. ignitus queens. The interspecific hybridization of a B. ignitus queen with a B. terrestris male produced either B. ignitus workers or the B. ignitus male phenotype, and the hybridization of a B. terrestris queen with a B. ignitus male produced B. terrestris males. Genetic tests using a portion of the mitochondrial COI gene for the parent and hybrid phenotypes indicated that mitochondrial DNA in the interspecific hybridization was maternally inherited. Our results indicated that interspecific hybridization occurred between B. ignitus and B. terrestris, which suggests that the hybridization will have a negative impact of competition and genetic pollution of native bumblebees.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제11권1호
• /
• pp.21-25
• /
• 2005

As a means for year-round rearing of bumblebee, CO$_{2}$ -narcosis time favorable for colony development was identified in Bombus terrestris. CO$_{2}$ -narcosis time divided into five classes: 11 days of adult emergence (A-11), the day of adult emergence (A-0), late pupal stage (LP), middle pupal sta ge (MP), and early pupal stage (EP). In egg-laying characteristics, the oviposition rate of LP, A-11 and A-0 was over 76.0$\%$, but that of MP and EP was less than 61.1 $\%$. At the same time, the days needed to first oviposition shortened to 9.8 ­10.5 days in A-11, A-O and LP, comparing to 13.7 -16.1 days in MP and EP. The rate of colony foundation, progeny-queen produced and period of colony foundation of A-11 were the best results in among those at different CO$_{2}$-treatment time. The number of worker produced was 109.2 -110.5 in A-H, LP and A-H, comparing to 82.0 - 86.8 in MP and EP. Also, the number of progeny-queen produced of A-H, A-O and LP was 36.1, 41.0 and 71.3, respectively, which corresponded to 1.5 - 3.1 fold higher than MP and EP. Taken these together, CO$_{2}$-narcosis time favorable for colony development was determined to be 11 days of adult emergence. Also, the day of adult emergence and late pupal stage showed a positive effect on the oviposition and colony development in CO$_{2}$-narcosis time.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제17권1호
• /
• pp.143-151
• /
• 2008

A muscle-specific lipase gene of the bumblebee Bombus ignitus was cloned and characterized. This gene, which we named Bi-Lipase, consists of seven exons encoding 317 amino acid residues. Bi-Lipase possesses all the features of lipases, including GXSXG consensus motif and Ser-Asp-His catalytic triad. Expressed as a 37-kDa polypeptide in baculovirus-infected insect Sf9 cells, recombinant Bi-Lipase showed an optimal pH of 9.0 and exhibited its highest catalytic activity at $40^{\circ}C$. Furthermore, through the addition of tunicamycin to the recombinant virus-infected Sf9 cells, recombinant Bi-Lipase was found to be N-glycosylated. Northern and western blot analyses indicated that Bi-Lipase was expressed in the wing, thorax, and leg muscles. These results show that Bi-Lipase is a muscle-specific lipase, suggesting a possible role of Bi-Lipase in the utilization of lipids for muscular activity in B. ignitus.