• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.103-107
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• 1999

The effects of MS salt strength, sucrose, and cultural conditions on bulblet formation and growth were investigated to optimize the conditions for micropropagating bulblets from in vitro bulb scales of Lilium Oriental Hybrid 'Casa Blanca'. There was no difference on bulblet formation in the range of 1/2~2 $\times$ strength of MS salt, but it was inhibited remarkably in 3 $\times$ strength of MS salt. The growth of regenerated bulblets was most stimulated on MS basal medium. Favorable bulblet formation and its growth from bulb scales were achieved when grown on the media with 1 : 2 or 1 : 3 in the ratio of NH$_4$^+ : NO$_3$^- , as well as on MS basal medium (NH$_4$^+ : NO$_3$^- = about l : 2). Therefore, MS basal medium was very suitable for bulblet formation and growth from bulb scales. Bulblet formation was inhibited but its growth was stimulated with increase sucrose concentration in the medium. The growth of regenerated bulblets was very effective on the media with 9~12% sucrose. Addition of activated charcoal (AC) to the medium inhibited bulblet formation from bulb scales, but enhanced the growth of regenerated bulblets. Especially, the medium containing 1 g/L AC was most effective on the growth of bulblets. No difference was found on bulblet formation and growth from bulb scales under light and dark conditions. In vitro micropropagation of L. Oriental Hybrid 'Casa Blanca' was supposed very reasonable to enhance the growth of the bulblets after forming of bulblets from in vitro bulb scales, and then, subculture the bulb scales from the grown bulblets on MS medium with 9% sucrose and 1 g/L AC.

• Korean Journal of Medicinal Crop Science
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• v.4 no.2
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• pp.119-125
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• 1996

To improve the efficiency of mass propagation in vitro of Fritillaria thunbergii, bulb scale and nodes were cultured in LS medium supplemented with the combination of 2, 4-D and kinetin or NAA and BA. The number and size of bulb, the number of adventitious shoot, the ratio of callus formation, rooting, and the effects of light and dark on the culture, plant regeneration from calli, and the gelling substances were investigated. The combination of 2, 4-D and kinetin in media was more effective than the media of NAA and BA for the bulblet formation. The media supplemented with 2 mg/L 2, 4-D and 1 mg/L kinetin, $1{\sim}2\;mg/L$ 2, 4-D without kinetin and $1{\sim}3\;mg/L$ BAA only were effective in the adventitious shoot development. Callus formation and root formation, respectively were effective in the medium supplemented with 2mg/L 2, 4-D and 1mg/L kinetin. In bulb formation, the medium with 5 mg/L kinetin was effective, and the most of bulbs were formed from the axillary bud of node part. In bulb formation, shoot growth, callus and root formation, the light culture for 16 hours per day was better than that in the dark culture. Bulb was nicely formed in the medium with 0. 2 mg/L 2, 4-D, 1 mg/L kinetin. The medium without hormone was most effective for plant regeneration. The phytagel was more effective than agar in the medium as the gelling agent.

• Korean Journal of Plant Resources
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• v.19 no.3
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• pp.432-435
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• 2006

A rapid and mass propagation method for multiple shoots and plant regeneration using bulb scales of Muscari comosum var. plumosum were developed. In vitro different parts of bulb scale as explants were cultured on 11 kinds of MS (1962) media supplemented with various plant growth regulators to induce shoot and callus. A combination of 2.0 mg/L 6-BA and 2.0 mg/L IBA on MS medium was the most favorable and induced the highest production (80%) of shoot formation after 30 days. We also found that the middle part of bulb scale was the best for mass propagation of Muscari comosum var. plumosum of which production could reach 64.4%.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.90-92
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• 2008

In 2007, a bulb rot of Allium victorialis var. platyphyllum Makino caused by Sclerotium rolfsii occurred sporadically in a herb farm at Gyeongsangnam-do, Korea. The symptom initiated with water-soaking lesion and progressed into bulb rot and wilt of a whole plant. Severely infected plants were blighted and killed eventually. White mycelial mats appeared on the lesion at early stage and a number of sclerotia were formed on the bulb. The sclerotia were spherical, $1{\sim}3 mm$ and white to brown. The optimum temperature for the growth and sclerotia formation was $30^{\circ}C$ on PDA. The hyphal width was measured $4-9{\mu}m$. The typical clamp connections were observed on PDA. Based on the symptom, mycological characteristics and pathogenicity to the host plant, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the bulb rot of A. victorialis var. platyphyllum caused by S. rolfsii in Korea.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.211-218
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• 1994

This experiment was conducted to obtain basic information for the establishment of in vitro initial culture system in Fritillaria thuubergii Miq. Methods of surface sterilization of scale segments as explant and effect of antibiotics added into the culture medium on contamination of explant and chilling treatment of mother bulb on bulblet formation were investigated. Portent of contamination of cultured scale segments was significantly higher in the outer scale segments which were unsuitable as initial culture explant than inner scale segments. Contamination of explants taken from inner scale of bulb was reduced by surface sterilizing explants in the solution of $4{\sim}5%$ sodium hypoclorite for $10{\sim}15$ mimutes. Addition of antibiotics such as kanamycin, vancomycia cefotaxim, agrirnycin and agreptomycin and dithane as fungicide and$lncyte^{tm}$ into MS medium was effective to reduce bateriological contamination, but did not work to control fungi. It had effective to delay the degree of contamination caused by fungi and bacteria haboring in cultured explants. Bulblet formation from cultured scale segments was promoted by dry storage for $2{\sim}4$ weeks or moisture storage of mother bulbs for $4{\sim}6$ weeks at $10^{\circ}C$ before excision of explants. Addition of kinetin into medium could not exerted for the bulblet formation from the scale segment of dry storaged bulb compared to control. But explant taken from 6 week moisture storaged bulb formed more than 10 bulblets per explant on the medium containing $3{\sim}5mg/L$ kinetin.

• Journal of the Korean Geotechnical Society
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• v.38 no.7
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• pp.25-37
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• 2022

In this paper, a small-scale model testing system was developed using a series of small-scale model tests to analyze the mechanism of compaction pile formation and evaluate the quality of controlled grading aggregates proposed as an alternative material to the sand compaction pile (SCP) method and granular compaction pile (GCP). These are the most typical ground improvement methods in field practice, particularly for soft grounds. However, the SCP has faced difficulties due to the supply shortage of natural sand and the corresponding price surge of sand. The GCP is limited in marine soft grounds because of the failure occurring at the pile tip caused by excessive expansion of the deeper bulbs, leading to uneven bulb formation. The uniformity of compacted pile bulbs is critical to ensuring the bearing capacity and quality of the compaction pile. This study aims to evaluate the performance of the new material and controlled grading aggregates using small-scale model tests simulating field compaction process to investigate its potential application in comparison with SCP. The compaction piles are examined in four cases according to different materials used for compaction pile and clay strength. The compaction pile materials, which are made of sand and controlled grading aggregates, used in this study were compared to reveal the mechanism of the bulb creation. The experimental data confirm that the bulb formation quality of the traditional sand and the new material, controlled grading aggregates are comparable. The compaction pile made of controlled grading aggregates presents higher bearing capacity than that of marine sand.

• Journal of Practical Agriculture & Fisheries Research
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• v.19 no.1
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• pp.75-82
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• 2017

This experiment was carried out to establish growth characterestics and number of bulblet, bulb weight, and diameter of bulblet on scale tissue culture in Lilium brownii. Flowering date of Lilium brownii was 25th, May with 50cm hight in field condition. Vimyl house condition was flowered 28th May with 54cm hight. Number of bulblet, bulb weight, diameter of bublet was good position of outer>Middle>inner scale in Lilium brownii. Number of bulblet formation was the best with 3.5 in large scale. Concentration of BA was the best at 1.5mg/L with 3.8 bulblet with bulb weight, weight and diameter of bulblet.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.45-45
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.323-323
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• 2005

• The Korean Journal of Ecology
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• v.26 no.5
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• pp.247-250
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• 2003

The investigation of ecological characteristics of Lycoris radiata was carried out in flooding (site I), half flooding (site II) and dry stands (site III). Seasonal change of air temperature showed similar patterns and that of light intensity showed quite difference among three L. radiata stands. Seasonal change of soil water content showed a great difference among three L. radiata stands. The study area was dominated by Pinus densiflora, P. thunbergii and L. radiata communities. The number of bulb in 1. radiata increased in September with bulb formation and decreased in January. Bulb weight in L. radiata was different from each site. The numbers of blossom were 23, 13 and 9, respectively in site I, II and III. The length of wreath were 17.0, 13.0 and 11.0cm, respectively, the length of stamen were 7.0, 6.4 and 6.5 cm, respectively and the length of stalk were 60.0, 45.0 and 42.0 cm, respectively in site I, II and III. The leaf of L. radiata developed rapidly in site I with sufficient water supply and lower light intensity, the number and the length of rootlets increased considerably in site III with insufficient water supply and higher light intensity, and the ecological characteristics in site II was intermediate between site I and site III. There was no great difference between the numbers of rootlets in site I and site II, which were due to sufficient water supply in two stands.