• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.126-132
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• 1997

Calcium carbonate ($CaCO_3$) immobilized with alginate was studied as buffer system to enhance the cultivation efficiency of Bifidobacterium longum (ATCC 15707) which is inhibited at low pH. To test the bufferring effect of the immobilized $CaCO_3$ beads, pH value in each modified trypticase-proteose peptone-yeast (TPY) broth which is adjusted to pH 4.0 with acetic acid, lactic acid and complex solution of acetic and lactic acid, 3:2 (M:M) was tested by concentration of $CaCO_3$ bead and reaction time. The bufferring effect of $CaCO_3$ bead became higher with increasing the amount of $CaCO_3$ bead in the acidic solution. The growth rate of bifidobacteria and bufferring effect were examined in relation to the amount of $CaCO_3$ bead and concentration of glucose in the modified TPY media. The growth rate of bifidobacteria and bufferring effect were increased with increasing the amount of $CaCO_3$ bead and concentration of glucose. Also, the exponential time of bifidobacteria became longer with increasing the amount of $CaCO_3$ bead and concentration of glucose in the modified TPY media. When we observed the growth rate of bifidobacteria by the method of pH-controlled culture and $CaCO_3$ buffer system, the $CaCO_3$ buffer system was more effective than that of pH-controlled culture. Therefore, this $CaCO_3$ buffer system may be useful as a method to enhance of the cultivation efficiency of bifidobacteria.

• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.611-616
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• 2008

The investigation was accomplished on the test area in Forest Practice Research Center in order to compare Point Grid method with Bufferring method in process of the calculation of the Actual Mean Skidding Distance with the GIS aided harvesting method. the investigation site amounted to 264.9 ha in area and 32.67 m in the road density, 306.1 m in road spacing. the Net Correction Factor (Kn) was 1.53 in both sides skidding. the theoretical mean skidding distance was 72.52 m. the shortest mean skidding distance amounted to 149.57 m with point grid method and 139.68 m with Buffering methode. skidding distance correction factor (Kr) was 2.15. actual mean skidding distance amounted to 251.51 m and factor of wood assortment skidding (Kg) was 3.29. when the tractor (Dongyang) and tower yader (Koller 303) are used on investigation site, the logging cost is calculated 11,808 Won with Tractor below 25% slope and 12,336 won with Tractor between 25~55% slope, 10,020 won with Tower Yarder more than 55% slope.