• Korean Journal of Plant Resources
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• v.28 no.3
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• pp.357-362
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• 2015

Carbohydrate sources are one of important factors associated with macro- and micro nutrients and phytohormones in vitro culture medium for shoot growth. The optimal carbohydrates for eight species of the genus Prunus which are economically important fruit crop was evaluated at the initiation and elongation stages. All carbohydrate seemed utilized for the bud break and leaf growth at the early stage of culture. However, shoot elongation and fresh weight of species tested were superior in the medium containing 90 mM of fructose or glucose rather than sucrose. There was no difference between glucose and fructose. Adventitious shoots from the axillary buds were induced in most species but no significant differences were observed except for two species (P. salicina ‘Shiro’ and P. tomentosa). These result demonstrated that glucose and fructose were suitable carbohydrate sources for diverse Prunus species than sucrose, although the response to the carbohydrates in the medium were slightly different in the species.

• Journal of Plant Biology
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• v.37 no.1
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• pp.17-25
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• 1994

Two potato (Solanum tuberosum L.) cultivars were transformed by Agrobacterium tumefaciens harboring cauliflower mosaic virus (CaMV) 35S promoter and $\beta$-glucuronidase (GUS) gene. Expression patterns of the CaMV 35S promoter according to tissue types and developmental stages, and genetic stability of GUS gene were investigated in the clonal progenies of transgenic potatoes. Kanamycin-resistant shoot emerged from tuber disc after 4 weeks of culture, and root was induced 6 weeks after culture on the selection medium. Shooting frequency of cvs. Superior and Dejima were 43% and 27%, respectively. Mature transformants and their clonal progenies showed no phenotypical abnormality. GUS activity was expressed primarily at parenchymatous cells of phloem tissue around the vascular cambium in the stem and root, and higher activity was found at the apical meristem of shoot, root and adventious shoot bud. GUS activity was higher at tubers of young explants than at stored tubers. These facts indicate that expression level of the CaMV 35S promoter differed according to tissue types and developmental stages of the organs. The GUS gene was stably inherited to each clonal progeny and normally expressed.

• Journal of Korean Society of Forest Science
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• v.89 no.4
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• pp.522-526
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• 2000

We tried to mass-propagate Prunus yedoensis from Jeju through tissue culture. We investigated the effect of bud collection time, the concentration of $NH_4NO_3$ in media and plant growth regulators(BAP, $GA_3$ and IBA) on the differentiation of winter buds. Buds, taken in February, flushed well regardless of various in vitro conditions. Bud flushing rate was significantly different depending on the collection time. BAP appeared to be effective on bud flushing. Sixty percent of buds taken in October flushed on the media containing $3.0mg/{\ell}$ BAR. No buds flushed on the medium supplemented with IBA. Buds, after flushing in BAP media, grew as foliated shoots and showed a rosette of leaves. When $GA_3$ supplemented to the BAP-containing media as a higher concentration than that of BAP, shoots elongated and developed into normal shoots. The combination of BAP $1.0mg/{\ell}$ and $GA_3$ $2.0mg/{\ell}$ is most recommendable for shoot elongation.

• Korean Journal of Agricultural Science
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• v.14 no.2
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• pp.205-212
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• 1987

To examine the morphogenetic response, stem segments of 8 Populus spp. and 3 different explants of P. nigra var. italica were cultured on MS (Murashige and Skoog 1962) and WPM (Woody Plant Medium) medium containing various phytohormones. The results obtained were as follows: 1. Shoot regeneration and development from stem segment of 8 Populus spp. showed a quite difference according to the section and the species. All of the species of Leuce and Tacamahaca section did not form adventitious buds, while most of explants showed axillary or dormant bud elongation after 4 weeks. But P. nigra var. italica of Aigeiros section showed a successful adventitious bud formation (mean 5.4 buds per explant). 2. Leaf, petiole, and internode segment of P. nigra var. italica showed a quite differences according to media and ex plants upon the morphogenetic response. Adventitious bud formation from leaf was more abundant and readily initiated on the abaxial side than on the adaxial side. Mean number of 103 adventitious buds per explant was obtained from abaxial side of leaf segment cultured on WPM medium containing $0.2mg/{\ell}$ BAP for 5 weeks. 3. 2,4-D (2,4-dichlorophenoxy acetic acid) supplemented to media appeared to be negative upon the adventitious bud formation of P. nigra var. italica, while it promoted callus formation from all explants. Especially, NAA (${\alpha}$-naphtalene acetic acid) or NAA combination with BAP (6-benzylaminopurine) promoted root regeneration from the all explant of P. nigra var. italica in this study.

• The Journal of Natural Sciences
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• v.8 no.1
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• pp.71-80
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• 1995

This experiment was conducted to investigate the effect of raising seedling methods of cold water, low night temperature, and abandoned mine on forcing culture of strawberry. The results were as follows ;1. $17^{\circ}C$ cold water alone and cold water plus 8-hour short day treatments significantly accelerated the flower bud differentiation of Nyoho cultivar to 11 and 15 days, respectively, comparing to common soil raising seedling.2. The date of flower bud differentiation of Nyoho cultivar as influenced by cold water treatment the 17th, the 13th and the 10th of September, respectively, when the seedlings were treated on the 10th of September and on the 25th and the 10th of August in 1991. In 1992, data showed that the flower bud differentiation dates were the 4th and the 2nd of September and the 29th of August when cold water treatment was done on the 10th of August and on the 25th and the 10th of July.3. The first harvest date when Nyoho cultivar was treated by cold water for 30days from Jul. 25 was Nov. 10, this implying that the harvesting day could advanced to 19 days comparing to that by the common soil raising seedling method. The resulting yield was recorded to 21.94 ton per hectare.4. Regardless of the starting date of the treatment, $13^{\circ}C$ low night temperature plus 8-plus short day treatments for 20 days required 17 days to differentiate the flower bud of Nyoho cultivar. Harvesting day could be advanced to 59 days comparing to that by the common raising seedling method when the seedling was treated on July 25, this resulting in increasing the total yield obtained by April 14 of following year to 8.25 ton per hectare.5. When the seedlings of both Nyoho and Hokowase cultivars were raised under the condition of abandoned mine, flowering and harvest date were earlier by the treatment for 20 days than that for 30 days or 40 days. The highest yiesd obtained was recorded in Nyoho cultivar or 1.88 ton per hectare.

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.173-178
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• 2010

An efficient protocol for shoot regeneration was developed for sesame (Sesamum indicum L.) internodes using the transverse thin cell layer (tTCL) culture method. The frequency of shoot regeneration and the number of adventitious buds produced from regenerated shoots depend significantly on explant age, thickness of the tTCL sections, and the phytohormones supplemented to the culture medium. A combination of 6-benzyladenine (2.0 $mg\;l^{-1}$) and a-naphthaleneacetic acid (0.5 $mg\;l^{-1}$) was found to be the best phytohormone combination for shoot bud induction, with the maximum number of shoots obtained when the tTCL sections were 0.5-1.0 mm thick and derived from 4- to 6-week-old seedlings of sesame. Well-developed shoots were rooted on MS medium without phytohormones, and 80% of the regenerated plantlets were successfully established in soil.

• Animal cells and systems
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• v.2 no.2
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• pp.229-232
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• 1998

Lysophosphatidylcholine (LPC) has been reported to be responsible for the sustained activation of protein kinase C (PKC). As chondroqenesis is known to be regulated by PKC, this study was performed to investigate the effects of LPC on chondrogenesis of chick limb bud mesenchymes in vitro. LPC treatment of mesenchymes during micromass culture significantly enhanced chondrogenic differentiation. The most effective time of LPC on the stimulation of chondrogenesis was the first day of micromass culture. Analysis of LPC effects on the expression of PKC isoforms revealed that LPC treatment increased expression of PKCa, among the multiple PKC isoforms, in the membrane fraction on day one of culture. The stimulatory effect of LPC on chondrogenesis was abolished if PKCa was down regulated by the prolonged treatment of cells with phorbol ester. The results suqqest that LPC promotes chondrogenesis through the activation of PKCa at the early stage of chondrogenic differentiation.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.131-135
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• 1995

Physiologically modified stem nodes derived from ex vitro and in vivo explants of hybrid aspen (Populus alba L.X P.grandidentata Michx. 'Crandon') were tested for their multiple shoot regeneration capacity using a broad spectrum dosage of cytokinins. Ex vitro derived stem nodes with excised axillary buds at the time of culture produced 11 to 13 multiple shoots on 20 to 30 $\mu$M zeatin containing Woody Plant Medium (WPM) after 6 weeks. Excision of axillary bud sprouts after 2 weeks of culture and culture of the remaining stem nodes on WPM with 1.0 to 2.0 $\mu$M BA or 10 to 30 $\mu$M zeatin produced 13 to 15 and 7 to 8 shoots per explant, respectively, Multiple tiny shoots were produced when in vivo derived stem nodes (on which all leaves were removed) were cultured on WPM with 30 to 50 $\mu$M 2iP or 20 to 50 $\mu$M zeatin. The greatest number of multiple tiny shoot proliferation (32 to 50 shoots per explant) were obtained when the explants were cultured on media containing 20 $\mu$M zeatin. Successful transplanting of these multiple shoots into the greenhouse and/or nursery was achieved.

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.1-7
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• 1984

This study was undertaken to find out the effects of three kinds of potting media and two sources of explants on the survival and early growth of new plantlets of Poputus alba ${\times}$ P. grandidentata in the greenhouse. The results obtained can be summarized as follows; 1) Among three potting media, Terralite was best for early growth and survival of plantlets. 2) Like humidifier, an intermittent misting system can be effective in keeping relative humidity high for the plantlets. 3) Survival rates over 80% could be obtained if humidity was kept high during the hardening period. 4) During hardening period, the plantlets showed the juvenile characteristics such as smaller leaves, thinner stems, and shorter internodes. 5) There were no differences on morphological characteristics between the plantlets originating from axillary buds and the plantlets originating from multiple shoots while they were growing at the greenhouse. 6) The plantlets originating from bud culture grew normally comparing to regular cuttings.

• Korean Journal of Medicinal Crop Science
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• v.1 no.1
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• pp.63-69
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• 1993

Present experiment were attempted to examine in vitro multiplication throughbud culture of Cornus officinalis. Bud derived shoot formation was established successfully on Murashige and Skoog's medium supplemented with $0.5mg\;/\;{\ell}$ BAP(N-benzyl amino purine). The shoot proliferation increased on the Driver Kuniyuki Walnut medium containing $0.5mg\;/\;{\ell}$ NAA(Napthalene acetic acid) and $0.5mg\;/\;{\ell}$ BAP. Addition of 2,4-D(2,4-Dichlorophenoxy acetic acid) to the media produced excessive callus inducton. IAA(Indole-3-acetic acid) and IBA (Indole-3-bu-tyric acid) enhanced multple shooting, and NAA showed callus induction and multiple shooting. Shoot growth was enhanced supplemented with 3% sucrose, $2g\;/\;{\ell}$ activated charcoal, and 1 / 4MS in organic salts. However, root formation of proliferated shoots was low about 5%