• Title/Summary/Keyword: bubbling activity test

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Streptomyces coelicolor 의 Catalase 들의 분석

  • 김형표;이종수;하영칠;노정혜
    • Korean Journal of Microbiology
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    • v.30 no.4
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    • pp.291-298
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    • 1992
  • Srrepromycec. corlirolar produces at least 4 catalase activity bands with different electrophoretic mobilities on polyacrylamide gel which vary during development. Spores and mycelia at stationary phase produced all the activity bands(Cat1. 760 kr); Cat3-I, 170 kD: Cat3-2, 140 kD: Cat3-3. 130 kD; Cat4, 70 kD) except for Cat2 (300 kD). Mycelia at mid-logarithmic phase produced only Cat2 and Cat3-2 bands, and mycelia at late-logarithmic phase produced bands except Catl and Cat\ulcorner. Catalase-deficient mutants were screened in S. coelicalur by H201 bubbling test following NTG mutagenesis. Wc tested sevcral non-bubbling or slow-bubbling mutants for their catalase activities. The overall activities in cell extracts decreased more than 5 fold. Activity bands in native gel selectively decreased in intensity or disappeared. In all the non-bubbling mutants testcd, Cat3-2 band decreased significantly or disappeared. suggesting that Cat3-2 is the major catalase. The selective disappearance of bands in mutants suggest that each band is governed by different genes. We purified catalase activity from -:ell extracts obtained at late-logarithmic phase. Following chromatographies on Sepharose CL-4B. DEAE Sepharose CL-6B. Phcnyl Sepharose CL-4B. and hydroxylapatite columns. only the Cat3-2 activity was obtained. The native form of Cat3-2 has molecular weight of approximately 140 kD, judged by gel electrophoresis. Thc electrophoretic mobility on SDS-polyactylamide gel suggests that this enzyme contains 2 identical subunits of 67 kD.

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Effects of Diffusibility of Bubbling Tablet Herbicide Formulations for Paddy Rice (수도용 발포성정제 제초제의 확산성에 미치는 영향)

  • Kim, Man-Ho;Ryang, Kwang-Rok;Lee, Chang-Hyeuk;Shim, Jae-Weon;Kim, Kyung-Hyun;Yoon, Cheol-Su;You, Yong-Man;Pyon, Jong-Yeong
    • The Korean Journal of Pesticide Science
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    • v.9 no.4
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    • pp.401-410
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    • 2005
  • A series of studies involving formulation processes, bubbling activity test, diffusibility test and biological efficacy test was undertaken to develop Jumbo herbicide formulations in paddy rice field. Gas evolution speed from the tablets prepared by different organic acids was in the order of oxalic acid, malonic acid, citric acid, and tartaric acid. The total volume of evolved gas from the tablet and diffusibility of the active ingredient in the submerged water were increased with increase of water temperature; the volume from 1 g of tablet at 10, 15, 25 and $30^{\circ}C$ for 5 minutes after immersion into water was 20, 25, 28, 45, 57 mL, respectively. The concentration of halosulfuron-methyl and pyriminobac-methyl in submerged water at 5, 15, 20 and $30^{\circ}C$ at the 2.4 m distance from the applied spot of the tablet was 20, 48, 85, and 97% of the concentration of treated spot, respectively. The evolved gas volume from the tablets was not affected by pH of submerged water. The concentration of halosulfuron-methyl in different sizes of submerged water within 24 hours after treatment of the tablet was maintained 0.16 ppm, which is ideal concentration at standard dosage regardless of the submerged water area. The concentration of pyriminobac-methyl was also uniformly dispersed in the water within 24 hours after applying it into the submerged water. The wind velocity of 5 m $sec^{-1}$ on concentration distribution of halosulfuron-methyl and pyriminobac-methyl in the submerged water 24 hours after treatment was not influenced; an equal concentration in the up the wind and down the wind from the applied spot was maintained. Spot treatments of one tablet formulations(5 g) including 4 times higher dosage at 4 different spots resulted in even concentration distribution of active ingredient in the water 24 hours after applying it into the submerged water.

A Study on Third-trimester Gravidas' Konwledge of Infant Care Activity (임신 말기 임신부의 영아 돌보기 지식에 관한 연구)

  • Lee, Su-Yeon
    • The Korean Nurse
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    • v.29 no.3
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    • pp.64-78
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    • 1990
  • The purpose of this study was to measure the degree of gravidas' knowledge of infant care activity, and to identify the factors influencing the difference of gravidas' knowledge. The subjects of this study consisted of 159 gravida visited 2 general hospitals, 2 OB/GY clinics and 2 midwives' clinics in J city for anenatal care. The data were collected from December 1 to 30, J.989. The instrument used for this study was true false type question which was developed by investigator through literature review. Data were analyzed by percentage, mean, t-test and ANOVA on significant difference with SPSS program. The results of this study were, summarized as follow: 1) The degree of gravidas' knowledge of infant care activity was 36.75, the degree of gravidas' know ledge of physical care activicy was 16.70, and the degree of gravidas' knowledge of psychosocial care activity was 20.00. 2) Among the physical care activity knowledge items, the diaper should be changed whenever it is soiled, $\lceil$shortly after feeding is over, bathing is bad$\rfloor$, $\lceil$gently patted or stroked on the back when bubbling$\rfloor$, $\lceil$10 minutes time in bathing is appropriate$\rfloor$, $\lceil$infant is crying whenever he is hungry per 3-4 hours$\rfloor$, and "room humidity controled 60 percent, more or less> were over a' percentage of 80 of right answer. Among the physical care, accivity knowledge items. $\lceil$a reason for burping was the air' he has swallowed will rise to the top of his stomach and be eructated$\rfloor$, $\lceil$burping is advisable after the feeding$\rfloor$, $\lceil$dressing of umbilical area is not necessary$\rfloor$, $\lceil$the thermometer should not be boiled for disinfection$\rfloor$, $\lceil$it is important chat the infant grasp the whole nipple within his mouth$\rfloor$, $\lceil$using alcohol sponge is bad whenever diaper is soiled", and $\lceil$when temperature is taken by the rectal method, infant legs shoud be grasped firmly_! were less than a percentage of 60 of right answer. 3) Among the psychosocial care activity knowledge items, $\lceil$it is nice, mother smile at bady frequently$\rfloor$, $\lceil$it is nice, mother praise a bady frequently$\rfloor$, $\lceil$it is nice, mother express bady's behavior$\rfloor$, $\lceil$talk frequently to the bady, the bady :should not be disturbed while feeding$\rfloor$, $\lceil$it is nice, mother see a baby face to face , $\lceil$it is nice, mother concentrate one's attention on her baby while playing$\rfloor$, $\lceil$it is nice, mother pat one's baby while he is feeding$\rfloor$, $\lceil$it is advisable, the baby is allowed to touch mother's breast while he is feeding$\rfloor$, $\lceil$it is advisable to stop feeding, if the baby protrudes one's tongue or cries while feeding$\rfloor$, $\lceil$baby is able to hear, see and smell$\rfloor$, $\lceil$it is nice, mother Concentrate one's attention on her baby while feeding$\rfloor$, and $\lceil$it is not advisable for mother to scold baby while he is crying out> were over a percentage of 80 of right answer. Among the psychosocial care activity knowledge items, $\lceil$Using' baby talks is bad when mother talks to baby> and $\lceil$it is nice, mother soothes the baby as soon as possible when he is crying I were less than a percentage of 60 of right answer. 4) There was statistically significant relationship between the degree of gravidas' knowledge of infant care activity and the demographic variables of gravida such as the age(p<0.01) and education level(p<0.01). From these results, it may be concluded that gravidas' knowledge of infant care activity is moderate level, and gravidas' knowledge of psychosocial care activity is greater than that of physical care activity. Also prenatal nursing educations should focus on subjects who have lower percentage of right answer in infant care activity knowledge items.dge items.

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