• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.15 no.3
• /
• pp.133-139
• /
• 2010

Marine algal flora and community structure were investigated at four sites in Kijang area on the southern east coast of Korea in August 2006 and August 2009, respectively. A total of 54 seaweeds including 6 green, 10 brown, 38 red were collected and identified. Of 54 seaweeds, 35 species were found throughout the survey period. Mean biomass in wet weight was from $616.0\;g\;m^{-2}$ to $1,462.4\;g\;m^{-2}$2 in 2006, and $354.8\;g\;m^{-2}$ to $965.6\;g\;m^{-2}$ in 2009. Maximum biomass was recorded at Mundong site, and minimum was recorded at Seoam (2006) and Dongbaek (2009) sites. The flora investigated (2006, 2009) could be classified into six functional groups such as coarsely branched form (58.7%, 58.1%), thick leathery form (10.9%, 11.6%), filamentous form (13.0%, 9.3%), crustose form (6.5%, 9.3%), sheet form (6.5%, 7.0%) and jointed calcareous form (4.3%, 4.7%) during survey period. The R/P, C/P and (R+C)/P values reflecting flora characteristics were 4.00, 0.75 and 4.75 at 2006, and 5.17, 1.00 and 6.17 at 2009, respectively. Therefore, the number of marine algae species and biomass in Kijang area were similar when they were comparing with the previous data. It suggest that any changes of seaweed diversity have not been observed in Kijang coastal area before and after the anthropogenic construction between 2006 and 2009.

• Economic and Environmental Geology
• /
• v.38 no.6 s.175
• /
• pp.675-687
• /
• 2005

The rocks of the five storied stone pagoda in the Jeongrimsaji temple site are 149 materials in total with porphyritic biotite granodiorite. They include pegmatite veinlet, basic xenolith and evenly developed plagioclase porphyry. This stone pagoda has comparably small fracture and cracks which are farmed in the times of rock properties, but surface exfoliation and granular decomposition are in process actively since the rocks are generally weakened from the influence of air contaminants and acid rain. Structural instability of constituting rocks in the 4th roof materials are observed to occur from distortion and tilt. Such instability is judged to threat stability of the upper part of the stone pagoda. Also, chemical weathering is operating even more as the contaminants, ferro-manganese hydroxides eluted from water-rock interaction on the rock surface. Most of the rock surface is covered with yellowish brown, dark black and light gray contaminants, and especially occur in the lower part of the roof rocks on each floor. The roof underpinning rocks are severe in surface pigmentation from manganese hydroxides and light gray contaminants. The surface of rocks lives bacteria. algae, lichen, or moss and diverse productions in colors of light gray, dark Bray and dark green. Grayish white crustose lichen grows thick on the surface with darkly discolored by fungi and algae in the first stage on basement rocks, and weeds grows wild on the upper part of each roof rocks. This stone pagoda must closely observe the movements of the upper part rock materials through minute safety diagnosis and long term monitoring for structural stability. Especially since the surface discoloration of rocks and pigmentation of secondary contaminants are severe, establishment of general restoration and scientific conservation treatment are necessary through more detailed study for this stone pagoda.

• Analytical Science and Technology
• /
• v.20 no.3
• /
• pp.227-236
• /
• 2007

This study was carried out to estimate the contents of heavy metals (Hg, Pb, and Cd) in seaweeds (n=169) which are closely related to food resources. The contents of heavy metals in the laver were found as follows; Hg was highly detected in the east coastal area ($0.0509{\pm}0.0635mg/kg$), Pb in the south coastal area ($0.0509{\pm}0.0635mg/kg$), Cd in the south coastal area ($1.3785{\pm}0.7754mg/kg$). In the sea mustard, Hg was highly detected in the south coastal area ($0.0152{\pm}0.0116mg/kg$), Pb in the east coastal area ($0.1633{\pm}0.2888mg/kg$), Cd in the south coastal area ($0.7141{\pm}0.4952mg/kg$). In the sea tangle, Hg was highly detected in the south coastal area ($0.0177{\pm}0.0118mg/kg$), Pb in the east coastal area ($0.0728{\pm}0.1025mg/kg$), Cd in the south coastal area ($0.1640{\pm}0.1253mg/kg$). In the sea lettuce, Hg, Pb, and Cd were highly detected from the west coastal area. In the fusiforme, Hg and Cd were highly in the west coastal area, Pb in the south coastal area. In general brown algae showed the highest level of Hg, Pb, and green algae showed the highest level of Cd Additionally, the significant correlation was observed between Hg and Cd (r=0.513, p<0.01), between Cd and Pb (r=0.420, p<0.05). The weekly average intakes of Hg, Pb and Cd from seaweeds takes about 0.17~10.30 % of PTWI (Provisional Tolerable Weekly Intakes) that FAO/WHO Joint Food Additive and Contaminants Committee has set to evaluate their safeties.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.41 no.3
• /
• pp.287-294
• /
• 2015

In this study, we investigated the antioxidative effects of brown seaweed Ecklonia cava extract and its subfractions. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction of E. cava. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) of ethyl acetate fraction ($FSC_{50}=6.98{\mu}g/mL$) and aglycone fraction ($7.03{\mu}g/mL$) are similar to that of (+)-${\alpha}$-tocopherol ($8.98{\mu}g/mL$) which is a reference control. Reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, $OSC_{50}$) of the aglycone fraction ($OSC_{50}=14.48{\mu}g/mL$) on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay was the strongest among all extract and fractions. However, all samples showed lower antioxidant activities than that of L-ascorbic acid ($6.88{\mu}g/mL$) known as a powerful antioxidant. The protective effect of 50% ethanol extract on the $^1O_2$-induced cellular damage of human erythrocytes was dependent on the concentration from 5 to $50{\mu}g/mL$. Both ethyl acetate fraction and aglycone fraction showed strong cellular protective activities at $10{\mu}g/mL$, where the cellular protective effects (${\tau}_{50}$) of each fraction were recorded 442.0 min and 539.9 min, respectively. Three kinds of extract/fractions of E. cava showed much greater cellular protective activities at $10{\mu}g/mL$ than that of liposoluble antioxidant (+)-${\alpha}$-tocopherol (40.6 min) which is a reference control. These results suggest E. cava extracts and its fractions can be applied as an antioxidant ingredient in a field of cosmetics.

• Asian Journal of Turfgrass Science
• /
• v.20 no.2
• /
• pp.147-156
• /
• 2006

This study was initiated to evaluate the growth characteristics of seven creeping bentgrass cultivars in summer, 'Penncross' showed the worst visual quality, whereas 'Penn A-4' and 'Crenshaw' the best quality. 'Putter', which was maintained a fair quality during the test period, was regarded as a good cultivar because of no significant variation in summer as compared to the other caltivars. 'Crenshaw',' L-93' and 'Penn A-4' were greater in chlorophyll content and 'Penncross' lowest during the summer. Also, 'SR1020' had a low content of chlorophyll. 'Putter' greatly increased in chlorophyll content after fertilization. The highest shoot density($19.3/cm^2$) was found with 'L-93' in early August, followed by 'Crenshaw', 'Penn A-4', 'Putter', 'Dominant', and 'SR1020' in that order. However, 'Penncross' was lowest($15.7/cm^2$). As for a root length, 'L-93' was longest, being over an average 5.5cm. 'Penn A-4' and 'Putter' also showed good result in root growth. However, the root length considerably decreased with 'SR1020', 'Penncross' and 'Dominant' in summer. Brown patch was a serious disease for the most cultivars, except 'Penncross'. 'Dominant' had the most serious damage. 'Putter', 'L-93', 'Crenshaw', 'SR1020', and 'Penn A-4' were also greater in damage over the others. In regards of algae occurrence in summer, 'Penn A-4' had the least damage, while 'Dominant' the greatest. In conclusion, 'Crenshaw', 'Penn A-4' and 'L-93' were the best cultivars in terms of summer growth. Conversely, 'Penncross' was the poorest one. However, this study was conducted under the conditions of one-year old green. Accordingly, in-depth experiment should be done over several years to elucidate the characteristics of growth for the wide range of creeping bentgrass cultivars during the summer.

• Journal of Life Science
• /
• v.23 no.9
• /
• pp.1155-1162
• /
• 2013

Estrogen like activities were evaluated using ethanol and hot water extracts of gongjindan (GJD) and 3 types of brown algae by an in vitro detection system. Laminaria japonica and Undaria pinnatifida represented statistically significant (p<0.001) estrogen like activities, while GJD and Ecklonia stolonifera did not. Furthermore, activities of hot water extracts ($500{\mu}g/ml$) of Laminaria japonica and three mixture (Ecklonia stolonifera : Undaria pinnatifida : Laminaria japonica, 1: 1: 1) were stronger than that of $10^{-7}M$ $17{\beta}$-estradiol. These results suggest that Laminaria japonica contain estrogen like compounds. Based on these results, the prepared seaweed GJD were used for animal experiments about the effects on inhibition of platelet aggregation and serum lipid levels in ovariectomized rats. 9-week Old female SD-rats were randomly assigned to 3 groups as sham-operated rats, ovariectomized rats and ovariectomized rats that were treated with GJD. The rats were placed on prescribed diets for 5 weeks following ovariectomy. Total cholesterol and triglyceride contents on serum decreased in the SHAM group compared to the OVX-CON group. 5 weeks feeding of GJD resulted in significant lowering of triglyceride and a decreasing tendency of total cholesterol level. The level of HDL-cholesterol on serum increased significantly by feeding diets containing the GJD. The ability of platelet aggregation of group treated with GJD was less than that of the OVX-CON group. These results suggest that the beneficial effects of seaweed gongjindan may be used to improve menopausal syndrome.

• Korean Journal of Environment and Ecology
• /
• v.25 no.2
• /
• pp.156-165
• /
• 2011

It is selected seven sites for marine algal flora and community structure and investigated seasonally from December 2008 to November 2009 in Gogunsan Islands, the west coast of Korea. A total of 58 species including 10 green, 16 brown and 32 red algae were collected and identified. Among these species, 11 species were found throughout the year. Annual mean biomass in dry weight were 213.8 g $m^{-2}$ at Sinsido 1, 143.1 g $m^{-2}$ at Sinsido 2, 133.3 g $m^{-2}$ at Sinsido 3, 164.0 g $m^{-2}$ at Munyeodo, 116.9 g $m^{-2}$ at Seonyudo, and 145.1 g $m^{-2}$ at Jangjado. Maximum biomass was recorded in Sinsido 1, and minimum mean biomass was Sinsido 4. The dominant species based on biomass were Sargassum thunbergii, Ulva pertusa, Sargassum fusiforme and Corallina pilulifera. S. thunbergii was the representative alga occurred at all seasons. The flora investigated could be classified into six functional groups such as coarsely branched form (46.6%), filamentous form (27.6%), sheet form (17.2%), thick leathery form (3.4%), jointed calcareous form (3.4%) and crustose form (1.7%). The R/P, C/P and (R+C)/P value reflecting flora characteristics were 0.33~0.75, 1.11~2.50, 1.47~3.25, respectively. Diversity index (H') and dominance index (DI) indicated that stability of seaweed community of Gogunsan Islands was unstable and environmental conditions were bad. According to multidimensional scaling (MDS) and cluster analysis, Gogunsan Islands were divided into three distinct groups. The first group was Sinsido 2, Sinsido 3, Seonyudo and Muyeodo and the second group comprised Sinsido 1 and Jangjado and the other was Sinsido 4 due to meaningful difference in similarity.

• Journal of Life Science
• /
• v.26 no.5
• /
• pp.555-562
• /
• 2016

In this study, we investigated the effects of Undaria pinnatifida (UP), Petalonia binghamiae (PB) and Punctaria latifolia (PL) extracts on the inhibition of proliferation and apoptosis in human gastric and breast cancer cells. AGS, MDA-MB-231 and SK-BR-3 cells were treated with 0, 50, 100, and 200 μg/ml concentrations of the extracts to determine their anti-proliferative effects, using the MTT assay. The UP, PB and PL extracts inhibited proliferation of AGS, MDA-MB-231 and SK-BR-3 cells in a dose-dependent manner, and the PL extract was found to be the most effective. DAPI staining was also performed to determine changes in the cell nucleus. Further, the AGS, MDA-MB-231 and SK-BR-3 cells were treated with 0, 50, 100, and 200 μg/ml of only the PL extract. DAPI staining showed increased chromatin condensation, which is indicative of apoptosis, in the 200 μg/ml group. The expression of the Bax, Bcl-2, and PARP proteins in AGS, MDA-MB-231 and SK-BR-3 cells treated with the PL extract was also determined by western blot analysis. The expression of Bax (a pro-apoptotic protein) and cleaved-PARP was increased, whereas the expression of Bcl-2 (an anti-apoptotic protein) was decreased compared with the control. These findings indicate that the PL extract may have potential as an alternative anticancer drug and nutraceutical.

• Journal of Life Science
• /
• v.20 no.12
• /
• pp.1851-1858
• /
• 2010

Estrogen deficiency in peri- and postmenopausal women results in variety of neurovegetative, psychic and somatic symptoms, and may contribute to severe diseases within the aged female population. Ecklonia stolonifera (ES) is an edible brown algae traditionally used in fishery towns in Far East Asia. This study was performed to investigate the effects of ES extracts on blood flow and serum lipid concentration in ovariectomized rats. Weight-matched female Sprague-Dawley strain rats were assigned to four groups. Three groups were surgically ovariectomized (OVX). The fourth group was sham operated. Rats were randomly assigned to the following groups: sham-operated rats (Sham), ovariectomized control rats (OVX-CON), ovariectomized rats supplemented with ES extract at 50 mg/kg bw/day (OVX-ES50) and ovariectomized rats supplemented with ES extract at 200 mg/kg bw/day (OVX-ES200). Serum total cholesterol and triglyceride contents decreased in the sham group compared to the OVX-CON group. Six weeks feeding of ES extract resulted in a significant (p<0.05) lowering of serum triglyceride and a lowering tendency of total cholesterol level. The level of Hdl-cholesterol in serum increased by supplementation of ES extracts at 50 mg/kg and 200 mg/kg bw/day (p<0.05). Blood passage time of the ES extracts-supplemented group was higher than the OVX-CON group. The ability of platelet aggregation of groups treated with ES extracts was less than that of the OVX-CON group. These results suggest that the beneficial effects of ES extract may be used to possibly improve on the metabolic syndrome of menopausal women.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.46 no.1
• /
• pp.34-38
• /
• 2017

Myelophycus simplex Papenfuss, a type of brown algae, is known to be majorly distributed in along the southern coast of Korea and Japan. The purpose of this study was to investigate the effects of M. simplex Papenfuss methanol extract (MSPME) on melanogenesis in ${\alpha}$-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells. Melanin contents of B16F10 melanoma cells were decreased by 27, 41, and 59% in a dose-dependent manner, upon MSPME treatment at 100, 300, and $500{\mu}g/mL$, respectively. Tyrosinase activities in B16F10 melanoma cells were decreased by 18, 49, and 61% in a dose-dependent manner, upon MSPME treatment at 100, 300, and $500{\mu}g/mL$, respectively. MSPME suppressed expression of tyrosinase, tyrosinase-related protein-1, tyrosinase-related protein-2, and melanocyte-inducing transcription factor in B16F10 melanoma cells. Concentration of $50{\mu}g/mL$ of MSPME especially induced greater decreases in tyrosinase activity, melanin contents, and melanogenic enzyme protein expressions. This results indicate that MSPME inhibits melanin synthesis and tyrosinase activity, and M. simplex Papenfuss extract may be an ideal candidate as a skin whitening agent.