• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1673-1679
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• 2003

Two hundred forty 1-d-old Arbor Acres broiler chicks were used to investigate the effects of Cu (II)-exchanged montmorillonite (CEM) or montmorillonite on the growth performance, intestinal microflora, bacterial enzyme activities and morphology of broilers. The chicks were assigned randomly into three groups with 80 chicks per treatment. The three dietary treatments were basal diet only (control group), basal diet +1 g $kg^{-1}$ montmorillonite, and basal diet +1 g $kg^{-1}$ CEM. The results showed that the addition of CEM to the diet increased significantly the body weight and feed efficiency, but a similarly significant increase was not found in broilers fed the diet containing montmorillonite. Supplementing the CEM in the diet of broilers also decreased the numbers of Clostridium perfringens and Escherichia coli in the small intestine and cecum. The addition of either CEM or montmorillonite to the diet depressed the activities of $\beta$-glucosidase and $\beta$-glucuronidase in the small intestinal and cecal contents. Data of villus height and crypt depth for duodenum, jejunum and ileum indicated that dietary addition of CEM or montmorillonite improved the small intestinal mucosal morphology.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1451-1457
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• 2016

A comparative study was performed to investigate the efficacy of KiFAY as a feed additive on performance parameters, thyroid, and pancreatic hormone levels in broilers. Ninety birds (Vencobb 400) were randomly divided into three groups viz., Control (no DL-methionine supplementation), Treatment1 (containing added DL-methionine) and Treatment 2 (containing KiFAY and without DL-methionine supplementation). The performance parameters (weekly body weight, body weight gain, feed intake, and feed consumption ratio) were recorded and calculated during the whole study of 4 weeks. Analyses of insulin and insulin-like growth factor (IGF 1), triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) were performed at the end of the study. The results show that birds on supplementation of KiFAY performed significantly (p<0.001) better than other treatments. The weekly body weight, body weight gain, feed in-take and feed consumption ratio improved in KiFAY treated birds. The study found an increase in insulin and IGF1 levels (p<0.001) in KiFAY compared with the other treatments. Serum T3, T4, and TSH levels in the Treatment 2 were higher than other treatments (p<0.001). The KiFAY supplementation was able to improve performance with associated responses at a hormonal level in broilers.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.1
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• pp.69-74
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• 1996

There levels of dietary ME (3,000, 3,200 and 3,400 kcal/kg) and four levels of Total Sulphur Amino Acid (TSAA) (0.73, 0.83, 0.93 and 1.03%) were studied in the starter period (0-3 wks) of the broilers. Three levels of dietary ME (3,000, 3,200 and 3,400 kcal/kg) combined with four levels of TSAA (0.65, 0.72, 0.79 and 0.86%) were studied in the grower period (3-6 wks). The crude protein content of the diet of the starter period was 23% while the diet of the grower period was 20%. The performance data of the starter broilers indicated that the dietary energy levels had no significant effects on body weight gain, feed intake and feed:gain ratio. However, TSAA levels had a significant influence on the growth and feed parameters. The response pattern for the grower period was similar to the starter period. The present experiment showed that in the tropics the TSAA requirement for the starter period was between 0.83 to 0.93% which is similar to the values recommended by NRC while for the grower period the TSAA requirement was between 0.79 to 0.86% at all the three energy levels which is higher than the values recommended by NRC.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.262-269
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• 2008

The aim of this study was to determine the effects of breeder age and stocking density on performance, carcass characteristics and some stress parameters (H-L ratio, serum glucose, cholesterol and triglyceride levels, tonic immobility test (TI), antibody production, relative asymmetry (RA) and external appearances). This experiment was carried out with 705 one-day old male broiler chicks (Ross 308) obtained from three different ages of broiler breeder (32, 48 and 61 wks). Each age group was randomly divided into two stocking density groups (11.9 and 17.5 broilers per $m^2$) with 5 replications per group. The experimental period was 6 weeks. Broilers from 32 wk-old breeders had lower initial weight (p<0.001), body weight gain of the first 3 week of rearing (p<0.01), the percentage of abdominal fat (p<0.001) and serum cholesterol level (p<0.01); higher percentage of gizzard (p<0.01) and longer TI duration (p<0.001) than those from 48 and 61 wk-old breeders. Broilers reared at 17.5 b/m2 had lower final BW, body weight gain, feed consumption, feather condition and foot health (p<0.001), higher percentage of heart, H-L ratio, serum glucose and cholesterol levels (p<0.001), and longer TI durations (p<0.001). There were no significant interactions in examined parameters except for feed to gain ratio between breeder age and stocking density.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.4
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• pp.573-578
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• 2013

A total of 720 1-d-old male broilers (Arbor Acres) with the initial BW = $45{\pm}2$ g were used in a 32-d experiment. Birds were randomly allotted into 1 of 4 treatments according to a completely random block design. Dietary treatments included: i) CON (basal diet), ii) ANT (CON+0.5 g antibiotics/kg feed (bacitracin methylene disalicylate)), iii) BP1 (CON+0.25 bacteriophage/kg feed), and iv) BP2 (CON+0.5 g bacteriophage/kg feed). The BWG and FI were not affected by dietary treatments throughout the whole experiment, whereas BP2 treatment reduced (p<0.05) the feed conversion ratio compared with the CON treatment during 0 to 14 d. The birds fed bacteriophages at the level of 0.25 g/kg increased (p<0.05) the relative liver weight. The inclusion of antibiotic and bacteriophage significantly reduced (p<0.05) the Escherichia coli and Salmonella concentration in the excreta compared with the NC group. Broilers fed the BP2 treatment had higher (p<0.05) lactobacillus counts compared with those fed NC and PC treatments. In conclusion, dietary supplementation of 0.5 g/kg bacteriophages increased feed efficiency and liver weight without effects on the breast muscle properties; also pathogens were inhibited by addition of bacteriophages.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.629-634
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• 1997

An experiment was conducted to investigate the effects of equiprotein replacement of dietary fish meal (FM) with duckweed (DW) and soybean meal (SBM) on the performance of broilers. A total of 112 seven-day-old as hatched broilers were fed on 4 different iso-energetic (2,818 kcal/kg) and iso-nitrogeneous (20.2% CP) diets up to 56 days of age. Diet A was control with 12% FM. In diets B (3% DW + 13.5% SBM), C (6% DW + 11.5% SBM) and D (9% DW +10% SBM). All FM protein of control diet was replaced by DW and SBM. The replacement of dietary FM by DW and SBM depressed feed intake, live weight gain and feed conversion efficiency and increased production cost and thus affected profitability. All those growth parameters had a linear declining trend as the proportion of DW in the diet was increased. It may be concluded that complete replacement of dietary FM by DW and SBM should not be recommended for raising broilers.

• Korean Journal of Veterinary Service
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• v.35 no.2
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• pp.91-97
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• 2012

The strategy for infectious bursal disease (IBD) control and its success rate under field conditions depends on hygiene management, IBD field pressure, level, and variation in maternally derived IBD antibodies. This study investigated the level of IBD-specific antibody by ELISA and the prevalence of IBD virus by PCR in broilers, white-semi broilers, and Korean native chickens raised in Jeongeup, Jeonbuk. IBD-specific maternally derived antibodies were measured from 698 chickens and the mean titers of maternal antibodies were $3,572{\pm}1,402$ in broilers, $1,262{\pm}762$ in white-semi broilers, and $1,932{\pm}912$ in Korean native chickens. At 2 weeks after vaccination, the geometric mean antibody titers of broiler, white-semi broiler, and Korean native chicken were $582{\pm}427$, $3255{\pm}1,080$, and $1,023{\pm}499$, respectively. According to sequence analysis of the variable virion protein 2 gene, 4 isolates were found to be very virulent IBDV, 9 isolates classical virulent, and 2 isolates intermediate plus vaccine strain.

• Korean Journal of Veterinary Research
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• v.45 no.1
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• pp.71-74
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• 2005

Pseudomonas aerusinosa infection was diagnosed in broiler chicks, and was submitted to the National Veterinary Research and Quarantine Service in Korea. The total mortality rate was about 1,500 birds out of 22,000 broilers. Clinically, affected birds showed clinical signs including depression and anorexia with lameness and trembling of the leg. At necropsy, the dead broilers appeared to have omphalitis, yolk sac infection, fibrinous epicarditis, and fibrinous exudates in liver with swollen hock joint. Microscopically, there were multiple necrotic foci in the liver, fibrinous exudates in the heart, and infiltration of heterophils into the joint spaces of the hock joint. Pseudomonas aerusinosa was isolated from the heart, liver and hock joint, and the isolate was named P-200. In effort to estimate the virulence of P-200, 1-day-old chicks were challenged intramuscularly and intrayolksacally with the isolate. On the basis of mortality rate, the isolate P-200 was found to be highly virulent. This is the first report of an occurrence of Pseudomonas aerusinosa infection in broilers in Korea.

• Food Science of Animal Resources
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• v.21 no.1
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• pp.24-31
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• 2001

The effects of addition of activated carbon to diet of broiler on productivity and meat quality of broilers were investigated. 48 broiler raised for six week. The addition level of activated carbon to each group was added 0, 0.6, 0.9 and 1.2%, respectively. During the experimental feeding period, weekly gain and feed intake of treatment fed diets contain 0.6 and 0.9 percent activated carbon were higher compared with those fed on control diet, though effects of diets containing graded levels of activated carbon on the feed efficiency were not found. When broilers were fed activated carbon on crude protein level of birds were higher compared with that of control diet. Also, crude fat of broilers fed diet containing activated carbon were shown to decrease compared with those fed of control diet(p<0.05). The pH from activated carbon diets was rather higher than that of control(p<0.05). The content of VBN and TBARS was not significantly different among all treatments. The heating loss has tend to decrease in activated carbon diet groups(p<0.05). The WHC tend to be increase in activated carbon diet groups(p<0.05). Blood cholesterol was no significantly different.

• Animal Bioscience
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• v.35 no.8
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• pp.1195-1204
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• 2022

Objective: The objective of this study was to investigate the in vitro fermentation profiles of different soybean oligosaccharides (SBOs) and their effects on skatole production and cecal microbiota of broilers. Methods: Five SBOs with varying main component contents were fermented using an in vitro batch incubation inoculated with broiler cecal microbiota. Gas production was recorded automatically, skatole, indole and short-chain fatty acids (SCFAs) were determined using high-performance liquid chromatography, and microbial changes were analyzed using 16S DNA gene sequencing. Results: The addition of SBOs increased (p<0.05) gas production, suggesting bacterial growth-stimulating activities. In addition, the concentrations of indole were significantly (p<0.05) decreased after SBO supplementation, and SBO III, with higher sucrose and stachyose contents, decreased (p<0.05) the skatole level. Our results also revealed that the fermentation of SBOs by cecal microbiota produced (p<0.05) SCFAs, which were dominated by propionic acid, butyrate acid and lactic acid compared to the control. In addition, SBO III increased (p<0.05) the abundance of Firmicutes and Subdoligranulum and decreased that of Bacteroides. Conclusion: These results suggest that SBOs with higher sucrose and stachyose contents are promising prebiotics in modulating gut microbiota and reducing odor emission in broilers.