• Title/Summary/Keyword: breeding lines

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CMV-P1 Resistance Evaluation Using Enzyme-linked Immunosorbent Assay of Pepper Genetic Sources (Capsicum spp.) (Enzyme-linked Immunosorbent Assay를 이용한 CMV-P1 저항성 고추 유전자원 평가)

  • Shin, JiEun;Xu, Sheng Jun;Kim, Jun Young;Woo, JeHyeon;Kim, Han Gil;Park, Yong Ju;Hong, Sae Jin;Kim, Byung Sup
    • Horticultural Science & Technology
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    • v.31 no.6
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    • pp.764-771
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    • 2013

  • Recently, Cucumber mosaic virus (CMV)-P1 infection in pepper cultivation is very serious problem, which causes low marketability and yield. In this research, 56 domestic pepper cultivars including 20 PR (Phytophthora resistance) cultivars, 31 foreign pepper lines collected from USA and 112 genetic pepper resources form RDA Genebank were used for CMV-P1 resistance evaluation. Resistance evaluation was performed at 24 days and 51 days after artificial inoculation of peppers by enzyme-linked immunosorbent assay (ELISA). Among the 36 domestic cultivars 'Muhanjilju' were resistant to CMV-P1. All 20 PR cultivars and 29 foreign peppers except 'NuMex Twilight' and 'Chainese Giant' were susceptible. Among 112 pepper lines from RDA Genebank, nine pepper lines were resistant, and 17 pepper lines were moderately resistant, and 86 pepper lines were susceptible. Almost all domestic peppers on the market were highly susceptible to CMV-P1, whereas 17.2% of foreign pepper cultivars and genetic pepper lines from RDA Genebank were resistant or moderately resistant. Resistant pepper lines selected in this study can be used as genetic sources for breeding CMV-P1 resistant pepper.

  • https://doi.org/10.7235/hort.2013.13021 인용 PDF KSCI

Non-pungent Capsicum Contains a Deletion in the Capsaicinoid Synthetase Gene, which Allows Early Detection of Pungency with SCAR Markers

  • Lee, Choong-Jae;Yoo, Eun Young;Shin, Joo Hyun;Lee, Jemin;Hwang, Hee-Sook;Kim, Byung-Dong
    • Molecules and Cells
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    • v.19 no.2
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    • pp.262-267
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    • 2005

  • The capsaicinoid synthetase (CS) gene cosegregated perfectly with the C locus, which controls the presence of pungency, in 121 $F_2$ individuals from a cross between 'ECW123R' and 'CM334', both of Capsicum annuum. We concluded that CS and C are tightly linked. Sequence analysis of the genes of four pungent and four non-pungent pepper lines showed that the non-pungent peppers had a 2,529 bp-deletion in the 5' upstream region of CS. We have developed molecular markers of the C locus to detect pungency at the seedling stage. Based on the deleted sequence, we developed five SCAR markers, two of them being codominant. These SCAR markers will be useful for easy, accurate, and early detection of non-pungent individuals in breeding programs.

  • KSCI

Developmental Changes of Recessive Genes-mediated Cucumber mosaic virus (CMV) Resistance in Peppers (Capsicum annuum L.)

  • Min, Woong-Ki;Ryu, Jae-Hwang;Ahn, Su-Hyeon
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.235-240
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    • 2014

  • Cucumber mosaic virus (CMV) is one of the most important viral diseases in pepper (Capsicum annuum L.), and several genes for resistance were reported in Capsicum spp. In Korea, a single dominant gene that is resistant to $CMV_{Fny}$ and $CMV_{P0}$ has been used for breeding. Recently, a new strain ($CMV_{P1}$) was reported that could infect cultivars resistant to both $CMV_{Fny}$ and $CMV_{P0}$. Therefore, breeding of more robust CMV-resistant cultivars is required. In this study, we surveyed the inheritance of $CMV_{P1}$ resistance and analyzed the location of the resistance loci. After $CMV_{P1}$ inoculation of various germplasms and breeding lines, one accession (ICPN18-8) showed no visual symptoms at 15 dpi (days post inoculation) but was susceptible after 45 dpi, and one resistant line (I7339) showed resistance until at 45 dpi. The latter line was used for tests of resistance inheritance. A total of 189 $F_2$ plants were examined, with 42 individuals showing resistance at 15 dpi and a phenotype segregation ratio close to 1:3 (resistant:susceptible plants). In a lateral ELISA test at 45 dpi, 11 plants showed resistance, and the segregation ratio was changed to 1:15. These results indicate that resistance in C. annuum 'I7339' is controlled by two different recessive genes; we named these resistance genes 'cmr3E' and 'cmr3L,' respectively. To locate these two resistant loci in the pepper linkage map, various RAPD, SSR, and STS markers were screened; only nine markers were grouped into one linkage group (LG). Only one RAPD primer (OPAT16) was distantly linked with cmr3E (22.3 cM) and cmr3L (20.7 cM). To develop more accurate markers for marker-assisted breeding, enriching for molecular markers spanning two loci will be required.

  • https://doi.org/10.7235/hort.2014.13092 인용 PDF KSCI