• 한국육종학회지
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• 제43권1호
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• pp.81-91
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• 2011

야생벼은 재배벼의 친환경적성을 강화시킬 수 있는 병해충 저항성 및 불량환경에 견딜 수 있는 유용한 유전자들의 보고로 알려져 왔다. 국내에서 육성된 벼 품종인 '화성'(AA게놈)와 야생벼인 Oryza. minuta(BBCC 게놈; Acc.=101141)간의 교잡을 통하여 종간잡종 후대들이 육성되었다. 불화합성과 초기분리세대의 극심한 불임을 극복하기 위해 배주배양으로 $F_1$ 개체를 확보하였으며, '화성'으로의 여교잡을 수 차례 실시하였다. 확립된 계통들에 대한 표현형 평가를 통하여 흰잎마름 병에 저항성을 발현하는 계통을 확인하고 '수원497호'라 명명하였다. '수원497호'와 '밀양23호'간의 교잡에서 작성된 $F_2$ 개체들을 유전자지도 작성 및 표현형조사에 활용하였다. 유전자지도 작성에 사용된 SSR 마커의 유전자형과 흰잎마름병균 접종에 따른 병반장길이간의 연관성분석을 수행하였다. '수원 497호'의 흰잎마름병저항성을 지배하는 주동유전자가 염색체 11번 말단에 표지 되었는데, 기존에 보고되어 온 흰잎마름병 저항성유전자들인 Xa3, Xa4, Xa26 및 Xa31 등이 표지 된 곳과 동일하였다.

• 한국연초학회지
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• 제17권1호
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• pp.27-32
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• 1995

A new flue-cured tobacco mutant line BU 8832-85 was developed at Taegu Experiment Station, Korea Ginseng and Tobacco Research Institute in 1994. KF 8832-85 was resulted from a cross of flue-cured cultivars NC 95$\times$NC 2326, and developed by a pedigree system of breeding ; initial selection was made by plant type and resistance to bacterial wilt(BW) disease(heudomonas solanaceamm) in the F2 generation under the natural field conditions infested with the pathogen. One white flowered plant was occurred by spontaneous mutation in a certain line among the F3 generatioin while the others were pink. Six plants from the seeds by selfing were selected at the field infested with the pathogen among 240 populations with white flowering in the F4, KF 8832-85 was selected based on yield and leaf quality trials among 6 lines in Fs generation. BCF 8832-85 was compared with its Parent for certain agronomic and chemical characteristics at Taegu Experiment Station in 1993 and 1994. The results showed that KF 8832-85 have white flower, the stalk height was approximately that of NC 2326 but averaged about loom taller than NC 95. It produced ground suckers as much as NC 95, and did not breakdown leaf at the same as WC 2326. KF 8832-85 have high resistance to bacterial wilt disease. Yield of KF 8832-85 was 10 and 18% higher then that of NC 2326 and WC 95, respectively. Price per Kg was equal to that of NC 2326. The contents of nicotine and reducing sugar did not differ significantly from NC 95, while total nitrogen was significantly lower than NC 95. Therefore, the new mutant line is genetically stable for agronomic and chemical characteristics and provides a source of bacterial wilt disease resistance for use in breeding resistant flue-cured cultivars. Key words : Mutant line, White flower, Spontaneous mutation.

• 한국자원식물학회지
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• 제18권3호
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• pp.424-432
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• 2005

신작물로 주목을 받고있는 괴경 형성 콩과식물인 아피오스(Apios americana Medikus)를 국내에 도입하여 특산작물로 개발하기 위하여 제주지역에서 2000년과 2001년도에 생육특성 조사와 재배 가능성을 검토하였다. 시험 재배한 두 계통(red-vine, green-vine) 모두 꼬투리 또는 종실은 형성하지 않고 지하부에 지하경이 비대하여 염주상의 지하 괴경을 형성하였으나 10a 당 수량은 $500\~800kg$ 사이였다. 계통별로는 red-vine이 green-vine보다 수량성이 높았는데, 주로 포기당 괴경수가 높은 것에 기인하였다. 파종시기별로는 4월 16일 파종시가 4월 1일 및 5월 1일 파종시보다 수량성이 높았다. 또한 지주재배가 무지주 재배보다 수량성이 높았다. 1년생의 경우도 $20\~80\;g$의 큰 괴경을 형성하는 주가 있어, 앞으로 특산작물화를 위해서는 다양한 용도개발과 아울러 우량 계통의 선발과 품종화가 중요할 것으로 판단되었다.

• 한국약용작물학회지
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• 제22권5호
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• pp.339-348
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• 2014

The transcriptomes of four ginseng accessions such as Cheonryang (Korean ginseng cultivar), Yunpoong (Korean ginseng cultivar), G03080 (breeding line of Korean ginseng), and P. quinquefolius (American ginseng) was characterized. As a result of sequencing, total lengths of the reads in each sample were 156.42 Mb (Cheonryang cultivar), 161.95 Mb (Yunpoong cultivar), 165.07 Mb (G03080 breeding line), and 166.48 Mb (P. quinquefolius). Using a BLAST search against the Phytozome databases with an arbitrary expectation value of 1E-10, over 20,000 unigenes were functionally annotated and classified using DAVID software, and were found in response to external stress in the G03080 breeding line, as well as in the Cheonryang cultivar, which was associated with the ion binding term. Finally, unigenes related to transmembrane transporter activity were observed in Cheonryang and P. quinquefolius, which involves controlling osmotic pressure and turgor pressure within the cell. The expression patterns were analyzed to identify dehydrin family genes that were abundantly detected in the Cheonryang cultivar and the G03080 breeding line. In addition, the Yunpoong cultivar and P. quinquefolius accession had higher expression of heat shock proteins expressed in Ricinus communis. These results will be a valuable resource for understanding the structure and function of the ginseng transcriptomes.

• 한국육종학회지
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• 제42권6호
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• pp.695-698
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• 2010

우리나라 수박 주산단지에 심한 피해를 주고 있는 수박 탄저병에 대한 저항성 계통 육성을 위하여 국내 외에서 수집한 수박 유전자원의 병 저항성 검정으로 탄저병 저항성이 강한 'AU-Producer'를 선발하여 저항성 친으로, 고품질 계통인 '920533'을 반복친으로 1993년 인공교배를 하였으며 1994년부터 1998년까지 BC4F1세대를 진전하였다. 1998년부터 2001년까지 원예적 특성 고정을 위해 6회 자가수분하여 세대를 진전하였으며 원예적 특성이 우수한 것으로 최종 선발된 'WCR16-1C-5C'를 농촌진흥청 농작물 직무육성 신품종 선정위원회를 거쳐 '한결'으로 명명하였다. '한결'은 수박 탄저병 race1과 3에 저항성이며 식물체 생장형은 덩굴성이며 주지길이는 중간정도이다. 평균과중은 5.3~10.1 kg 범위로 중대과종이며, 평균 과피 두께는 1.2~1.6 cm로 시판품종 등과 유사하였다. 과피는 녹색이며 과피의 줄무늬는 중간정도이다. 과육색은 적색이며 당도는 9.8~$11.8^{\circ}Bx$. 범위이고 중생종으로 과실의 품질을 비롯한 원예적 특성은 대비 품종과 유사하였다. 수박 탄저병 저항성 계통 '한결'은 탄저병 race 1과 3에 강한 저항성이고, race 2에는 중간저항성으로 품종육성 재료로 이용하거나 노지 및 시설재배 품종으로 재배할 수 있을 것으로 기대되며 재배시 탄저병 방제 노력과 비용을 절감할 수 있을 것으로 예상된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.42-42
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• 2019

This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared Reflectance Spectroscopy) analysis data. The average waxy type amylose contents was 8.7% in landrace, variety and weed type, whereas 10.3% in breeding line. In common rice, the average amylose contents was 22.3% for landrace, 22.7% for variety, 23.6% for weed type and 24.2% for breeding line. Waxy type resources comprised of 5% of the total germplasm collections, whereas low, intermediate and high amylose content resources share 5.5%, 20.5% and 69.0% of total germplasm collections, respectively. The average percent of protein contents was 8.2 for landrace, 8.0 for variety, and 7.9 for weed type and breeding line. The average Variability Index Value was 0.62 in waxy rice, 0.80 in common rice, and 0.51 in protein contents. The accession ratio in arbitrary ranges of landrace was 0.45 in amylose contents ranging from 6.4 to 8.7%, and 0.26 in protein ranging from 7.3 to 8.2%. In the variety, it was 0.32 in amylose ranging from 20.1 to 22.7%, and 0.51 in protein ranging from 6.1 to 8.3%. And also, weed type was 0.67 in amylose ranging from 6.6 to 9.7%, and 0.33 in protein ranging from 7.0 to 7.9%, whereas, in breeding line it was 0.47 in amylose ranging from 10.0 to 12.0%, and 0.26 in protein ranging from 7.0 to 7.9%. These results could be helpful to build database programming system for germplasm management.

• 한국연초학회지
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• 제15권2호
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• pp.115-122
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• 1993

KB103 was developed from a cross between Burley 21 and Va 528 in 1983. It was developed by haploid method derived from anther culture method. It has moderate resistance to black shank(Phytophhorn nicotianae Breda de Haan Var. nictiana Waterhouse). KB103 produced good yields and quality in 1988-1990. It flowered about four or five days later than standard variety Burley 21, and plant height, leaf width, leaves per plants were similar to those of Burley 21. In maturing and curing properties as well as in chemical constituents of cured leaves, KB 103 was very similar to that of Burley 21. Regional farm test with the breeding line, KB 8304- ADH 65 named as KB 103 in 1986, was performed for 4 crop years from 1987 at 29 locations. It was released on burley tobacco area in 1992.

• 한국작물학회지
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• 제65권3호
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• pp.161-171
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• 2020

본 연구에서는 국내 벼 직파재배 활성화 및 재배면적 확대를 위하여 담수발아 관련 QTLs 단편이 이입된 자포니카형 우량 계통을 육성하였다. 더불어 담수 중 혐기발아 내성과 관련 분자표지를 개발하였으며, 이를 우량계통 선발에 직접 활용하였다. 인공교배를 통해 국내 자포니카 잡초벼인 PBR을 수여친으로 남평을 반복친으로 여교잡을 수행하였으며, 이 조합으로부터 담수 혐기 발아성이 개선된 자포니카 형 우량계통을 육성하였다. 그리고 혐기 발아 내성 관련 QTL 판별을 위한 CAPS 분자표지(NP01.014, NP03.077, NP11.091)를 개발하였다. 여교잡 집단을 육성하고, 작물학적 특성, 담수저항성 검정 등의 표현형 선발과 분자표지 선발을 통해 주요 농업형질, 담수 혐기 발아성이 양호한 5계통이 최종 선발되었으며, 이들 계통은 모두 QC1 혹은 QC2 조합으로 남평에 비해 온실에서 평균 2.3배, 포장에서 2.6배 가량 유묘 생존율이 개선된 것으로 나타났다. 선발된 5계통에 대한 생산력 검정 예비시험(PYT), 주요 작물학적 특성 및 담수 혐기 발아성 등의 검정을 통해 28708을 담수직파 우량계통으로 최종 선발하고 '전주643호'로 계통명을 부여하였다.

• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.267-274
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• 2014

The immune system in teleost fish is not completely developed during embryonic and larval stages, therefore effective innate mechanisms is very important for survival in such an environment. However, the knowledge of the development of immune system assumed to be restricted. In many species, lysozymes have been considered as important genes of the first line immune defense. The early detection of lysozyme mRNA in previous reports, led to the investigation of its presence in oocytes. As a result, c-type lysozyme mRNA transcripts were detected in unfertilized oocytes indicating maternal transfer. Therefore, we investigated the expression patterns of lysozymes in flounder, including the matured oocyte. In our results, c-type lysozyme mRNA was first detected in unfertilized oocyte stage, observed the significantly decreased until hatching stage, and was significantly increased after hatching stage. On the other hand, g-type lysozyme mRNA transcripts were first detected at late neurula stage, and the mRNA level was significantly increased after 20 dph. It may be suggest that maternally supplied mRNAs are selectively degraded prior to the activation of embryonic transcription. This study will be help in understanding the maturation and onset of humoral immunity during development of olive flounder immune system.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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• pp.96-102
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• 1998

A lot of individual ginseng plants were selected in the farmer's fields to develop new ginseng varieties with desirable traits. Among them, a promising line has been developed through comparative cultivation of several lines selected with pure line separation of local raced. It was then designated as "KC (denotes Korean Ginseng)" and tested in the regional yield and adaptation trials for 10 years. KG lines grew vigorously after 4 years of age. Especially, KG 102 line arson운 them showed traits of multiple and short stems. As for the root characters, the length of taproot of KG 101 line was longer than that of local race, Jakyung-jong, or other lines ailed the root weight of KG 102 line waIn 15 % higher than that of local race. In general, KG 101, KG 103, KG 104, and Hwangsauk-jong had good root shape. Total amount of ginsenosides of ginseng taproot was the highest in KG 103 line than in local race and other lines. In these studies, we, elected three superior lines, KG 101, KG 102, and KG 103, having characteristic of good root shape, high yield, and large amount of ginsenoside, respectively.