• 생명과학회지
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• 제24권6호
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• pp.618-625
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• 2014

쌀가루는 많은 식품 가공에 이용된다. 그러나 밀가루 반죽이 빵과 면을 포함한 많은 식품 가공 제품에 적합한 반면에, 쌀로 만든 반죽은 신장성과 탄력성이 부족하다. 고분자 글루테닌 서브유닛(HMW-GS)은 밀의 가공 적성을 결정하는데 중요한 역할을 한다. 본 연구에서, 우리는 아그로박테리움(Agrobacterium) 동시 형질전환법을 이용하여 한국 밀 품종인 '조경'으로부터 HMW-GS를 암호화하는 밀 Glu-1Dy10 유전자를 발현하는 marker-free 형질전환 벼 식물체를 개발하였다. 오직 Glu-1Dy10 유전자와 HPTII (hygromycin phosphotransferase II) 저항성 유전자만을 포함하는 분리된 DNA 조각들로 구성된 두 가지 발현 카셋트(cassettes)를 독립적으로 아그로박테리움(Agrobacterium) EHA105 에 도입하였다. Glu-1Dy10 또는 HPTII를 함유하는 EHA105 를 각각 3:1 비율로 벼 캘러스에 접종하였다. 290개의 하이그로마이신(hygromycin) 저항성 $T_0$ 식물체 중에서 우리는 벼 게놈에 Glu-1Dy10과 HPTII 유전자가 모두 삽입된 29개의 형질전환 라인을 획득하였다. 우리는 Glu-1Dy10 유전자가 벼 게놈 내로 도입된 것을 Southern blot 분석을 통해 다시 확인하였다. 형질전환 벼 종자에서 Glu-1Dy10의 전사(Transcripts)와 단백질을 semi-quantitative RT-PCR과 Western blot 분석을 통해서 확인하였다. 최종적으로, 오직 Glu-1Dy10 유전자를 갖는 marker-free 식물체를 $T_1$ 세대에서 성공적으로 선발할 수 있었다.

• 한국식품영양과학회지
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• 제42권7호
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• pp.1103-1108
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• 2013

박력분을 이용해 제조한 쿠키 반죽에 microwave, 가압가열, microwave 및 가압가열 병행 처리 후 오븐에 굽기 전후의 쿠키의 일반 특성과 품질에 미치는 영향을 알아보았다. 쿠키의 pH는 microwave 1분 처리한 경우 무처리구에 비해 약간 증가하였고, microwave 1분 처리 후 구운 경우와 가압가열 및 microwave 병행처리 후 구운 경우 감소함을 보였다. 수분 함량은 반죽에 가압가열 처리했을 경우 상당히 증가하였다. 쿠키의 색도 측정 결과 외부색에서 명도는 무처리구에 비해 모든 처리구에서 감소하였고, 적색도는 microwave 1분 처리한 경우를 제외하고 모든 처리구에서 무처리구에 비해 높은 값을 보였으며 황색도는 microwave 1분 후 구운 경우를 제외하고 무처리구에 비해 낮은 값을 보였다. 물성 측정 결과, 경도, 검성, 씹힘성, 전단력에서 가압가열 및 microwave 병행처리 후 구운 처리구가 가장 많은 증가를 보였다. 관능평가에서는 무처리구가 전체 항목에서 가장 높은 점수를 받았고, microwave 1분 처리 후 구운 경우가 무처리구와 유사한 기호도를 보임을 확인하였으며, 선행 연구에서 밀 gliadin의 항원성을 감소시키는데 효과가 좋았던 가압가열 및 microwave 병행처리구의 경우 색, 형태, 질감, 전체적 기호도에서 낮은 점수를 받았다. 따라서 가압가열 및 microwave 병행처리구에 대해 색, 형태, 질감, 전체적 기호도를 개선시킬 수 있는 대책을 마련한다면, 알레르기성이 저감화된 제품 개발에 적합할 것으로 사료된다.

• 한국조리학회지
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• 제12권2호
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• pp.222-235
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• 2006

Herbs have unique tastes and flavors, and they have been used in western foods. This study used herbs to make cookies. The purpose of this study was to investigate the sensory characteristics of cookies with rosemary(1, 2, 3, 4%) and mints(1, 2, 3, 4%) after foretasting herbs. The sample of this study was 880 college students which consisted of 436 males and 444 females. The results of this study were as follows. This study was conducted to find a high quality product through a sensory test with rosemary and mints. First, in terms of foretasting experience, the respondents had tasted 12 herbs. 65.2% of them preferred parsley, followed by mint(45.2%), bay leaf(44.0%), rosemary(28.3%), basil(21.8 %), etc. Second, in terms of the most important items while making herb added to bread and cookies, taste was high with 45.8%, flavor 27.3%, price 10.1%, nutrition 8.5%, etc. There were significant differences between genders and among majors(p<0.001). Third, it was found that appearance was high with 2% added group(M=6.00) in terms of sensory preference. The flavors, tastes, texture, overall acceptability were high with 1% added group in terms of sensory preference of cookies with rosemary. The respondents showed low preference for cookies with mints. While the 1% added mint group showed the highest preference for appearances and texture, the control group showed the highest preference for flavors, tastes and overall acceptability. There was a significant difference in appearances and tastes on all samples except colors and taste of rosemary added cookies $(p<0.05{\sim}p<0.001)$. Fourth, males preferred 2% rosemary added one in appearances and tastes in a sensory test. They preferred the 1% rosemary added group in terms of flavors, texture, and overall acceptability. Females preferred appearances of cookies with 2% rosemary and also preferred flavors, tastes, texture and, overall acceptability with 1% rosemary. Both of them less preferred 4% mint added one. Overall, this study found that respondents preferred 1% rosemary herb cookies. There should be further researches with less than 1% mint to make herb cookies.

• Journal of Nutrition and Health
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• 제28권7호
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• pp.653-674
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• 1995

This study was aimed at comparing the dietary patterns of university female students according to their residence type. As for subjects, one hundred and twenty students of Kongju national university were chosen as a total. They were devided into three groups ; those who live in family home, or university dormitory, or self-boarding house. In this study, intakes of food and nutrient and dietary behaviors of each group were investigated through two-day food records and questionnaire. The result was that mean daily intakes of calcium and iron were lower than RDAs in all groups. According to residence type, subjects of family home and self-boarding seemed to have more serious problem in the nutrients mentioned above considering the proportions of less than 2/3 of RDAs. Especially, iron status was poorer than any other nutrients in the aspects of this proportion and food source. Iron was taken largely from vegetable foods which were recognized as a source of low bioavailability of iron. The average distribution ratios of breakfast, lunch, dinner and snack to total calorie intake was 22, 29, 30 and 19. Thus, it proved that breakfast tended to be more negligent than any other meals, and that snack was an important means to give nutrients for subjects. This meal pattern might be one of the factor for the decrease of the dietary quality. Processed foods were founded to be an important source for providing nutrients. Percentages of calorie, carbohydrate and fat consumed by processed foods to total nutrients. Percentages of calorie, carbohydrate and fat consumed by processed foods to total nutrients intake were 30.6, 29.5 and 46.2. However, other nutrient intakes from processed foods such as vitamin and mineral were small. And processed foods which give nutrients were mainly cereal products like ra myon, bread, and milk and milk products. Consequently, processed foods seemed to be selected as a combinient substitute food which supply calorie to fill their hunger. According to the living type, dormitory students had them more frequently than any other groups. Food habit score was within the category of poor or fair. Particularly, the score of self-boarding students was the lowest of groups(p<0.05). The major dietary problems were small or overating, missing meal and irregular eating. The degree of these problems was a little different among groups(P<0.01). Nineteen kinds of food were consumed per day, and family home and self-boarding students had foods less variously than dormitory students(P<0.05). Proportions of skipping breakfast, lunch and dinner were 20.8, 12.5 and 8.3%. Accordingly, breakfast was missed more often than any other meals. And self-boarding students missed breakfast more often than any other groups. As a conclusion, subjects should take more calcium and iron, and eat diverse foods to improve their nutritional status. And it is also necessary that breakfast should be regarded more importantly, and that processed foods should be taken considering nutritional balance. In view of residence type, subjects of family home or self-boarding had more dietary problems than dormitory subjects. Thus, these results suggest that university female students might have low ability of meal management, and dietary patterns were different by their residence type. Therefore, nutrition education for them should be carried out with respect to dewelling environment.

• 한국식품영양학회지
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• 제10권2호
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• pp.201-207
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• 1997

경기 북부 지역의 3개 전문대학에서 주, 약간별 학생들의 학교식당 이용실태를 조사하였다. 학교식당의 이용율에서 거의 매일 이용하는 주간 학생은 64.29%였고, 야간 학생들은 44.35%로 주간 학생의 이용이 더 많았다. 학교식당을 이용하는 이유로는 '달리 먹을 만한 장소가 없어서'가 주, 야간 각각 49.38, 68.01%로 나타났고, 다음으로는 '가격이 저렴해서', '시간이 절약되므로'의 순으로 나타났다. 학교식당을 이용하지 않는 이유로는 주간의 경우 52.09%, 야간의 경우 31.99%가 '맛이 없어서' 이용하지 않는 것으로 나타났고, 학교식당을 이용하지 않는 경우 주간의 경우 68.63%, 야간의 경우 37.37%가 학교 밖에서 매식하는 것으로 나타났다. 평균적인 식사의 형태는 밥식>라면, 국수류>과자류>빵류의 순이었다. 학교식당의 음식에 대한 만족도는 음식의 양을 제외하고는 맛, 기호, 반찬수 등에서 만족도가 낮은 것으로 나타났다. 학교식당의 개선점으로는 '음식의 맛과 질이 향상되어야 한다'는 점에 가장 많이 응답했고, 그 다음으로 '메뉴가 다양해야 한다', '가격이 저렴해야 한다', '위생적이어야 한다'의 순으로 나타났다.

• Applied Biological Chemistry
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• 제35권4호
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• pp.254-259
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• 1992

정어리버어거를 가공하기 위하여 쵸핑한 정어리 육에 대해 분리대두단백 : 물 : 대두유(16 : 80 : 45)로 만든 유화커어드 14.1%, 식염 1.5%, 모노글루탐산나트륨 0.1%, 설탕 2.0%, sodium bicarbonate 0.4%, 중합인산염 0.2% 빵가루 8.0%, 분리대두단백 3.0%, 양파가루 0.3%, 생강가루 0.15, 후추가루 0.1%를 첨가하여 정어리육 알맹이가 붕괴되지 않도록 혼합한 후 튀긴($165{\pm}2^{\circ}C$, 3분) 것이 가장 좋았다. 정어리버어거의 수분함량은 62.7% 조단백질 17.7%, 조지방은 14.9%로 유화커어드를 첨가하므로서 원료어 지질의 변동에 관계없이 조지방 함량은 $14{\sim}15%$ 범위로 조정 가능하였다. 제품의 주요 구성지방산은 16 : 0, 18 : 1, 20 : 5 및 22 : 6 등으로 고도불포화지방산이 많이 함유되어 있었고, aspartic acid, glutamic acid, leucine, lysine 등과 같은 아미노산의 함량이 많았다. 정어리버어거는 histidine을 주체로 하는 유리아미노산과 IMP를 주체로 하는 핵산관련물질이 주요한 맛성분이고 여기에 total creatinine, betaine 및 TMAO가 보조적으로 정어리버어거의 맛에 관여하리라 판단된다.

• Journal of Ecology and Environment
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• 제42권1호
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• pp.1-11
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• 2018

Background: In the study, the effects of elevated $CO_2$ and temperature on the nitrogen content, carbon content, and C:N ratio of seven rare and endangered species (Quercus gilva, Hibiscus hambo, Paliurus ramosissimus, Cicuta virosa, Bupleurum latissimum, Viola raddeana, and Iris dichotoma) were examined under control (ambient $CO_2$ + ambient temperature) and treatment (elevated $CO_2$ + elevated temperature) for 3 years (May 2008 and June 2011). Results: Elevated $CO_2$ concentration and temperature result in a decline in leaf nitrogen content for three woody species in May 2009 and June 2011, while four herb species showed different responses to each other. The nitrogen content of B. latissimum and I. dichotoma decreased under treatment in either 2009 and 2011. The leaf nitrogen content of C. virosa and V. raddeana was not significantly affected by elevated $CO_2$ and temperature in 2009, but that of C. virosa increased and that V. raddeana decreased under the treatment in 2011. In 2009, it was found that there was no difference in carbon content in the leaves of the six species except for that of P. ramosissimus. On the other hand, while there was no difference in carbon content in the leaves of Q. gilva in the control and treatment in 2011, carbon content in the leaves of the remaining six species increased due to the rise of $CO_2$ concentration and temperature. The C:N ratio in the leaf of C. virosa grown in the treatment was lower in both 2009 and 2011 than that in the control. The C:N ratio in the leaf of V. raddeana decreased by 16.4% from the previous year, but increased by 28.9% in 2011. For the other five species, C:N ratios increased both in 2009 and 2011. In 2009 and 2011, chlorophyll contents in the leaves of Q. gilva and H. hamabo were higher in the treatment than those in the control. In the case of P. ramosissimus, the ratio was higher in the treatment than that in the control in 2009, but in 2011, the result was the opposite. Among four herb species, the chlorophyll contents in the leaves of C. virosa, V. raddeana, and I. dichotoma did not show any difference between gradients in 2009, but decreased due to the rise of $CO_2$ concentration and temperature in 2011. Leaf nitrogen and carbon contents, C:N ratio, and chlorophyll contents in the leaves of seven rare and endangered species of plant were found to be influenced by the rise and duration of $CO_2$ concentration and temperature, species, and interaction among those factors. Conclusions: The findings above seem to show that long-term rise of $CO_2$ concentration, and temperature causes changes in physiological responses of rare and endangered species of plant and the responses may be species-specific. In particular, woody species seem to be more sensitive to the rise of $CO_2$ concentration and temperature than herb species.

• 한국지역사회생활과학회지
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• 제22권3호
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• pp.379-393
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• 2011

This study compared the college students' dietary behavior for processed foods, who will be the main consumers in the future and looked for the measures to understand and establish the right food labeling system by surveying the level of understanding and utilization of food labeling. The data was analysed by SPSS win 17.0 program, and the results are as follows. For the standard of selecting processed foods, the group with high nutrition knowledge considered the reliability of foods as important and the group with low nutrition knowledge considered the products introduced in TV commercial as important. When purchasing processed foods, the group with high nutrition knowledge considered nutrition, taste, price, appearance(shape), and the consumable period more than the group with low nutrition knowledge. For trans fat, the group with high nutrition knowledge learned more about it than the group with low nutrition knowledge. The ratio of confirming food nutrition label was higher in the group with high nutrition knowledge. Regarding the level of confirming individual food labels, the highest level was for milk and dairy products. And there was significant difference for the processed products of meat, cookies, bread and noodles. It was found that the level of confirmation was higher in the group with high nutrition knowledge. And the most important indication for individual food product was the consumable period. To preserve the purchased foods, the group with high nutrition knowledge preserve the foods in line with the description written on the food cover sheet, and this group used to return or exchange the products when they found them spoiled or purchased by mistake. The group with high nutrition knowledge knew more about the nutrition indication than the group with low nutrition knowledge. The necessity of nutrition indication for processed foods and the need of education and PR(Public Relation) were acknowledged higher in the group with high nutrition knowledge. For the effect of nutrition indication, it showed that the group with high nutrition knowledge thought it would improve the quality and the group with low nutrition knowledge thought it would be helpful when comparing the product with others. The group with high nutrition knowledge showed higher understanding level about nutrition indication than the group with low nutrition knowledge.

• 대한지역사회영양학회지
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• 제15권4호
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• pp.429-444
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• 2010

The purpose of this study was to examine the relationship between bone density and dietary intake for college women in their twenties. This study was performed on 160 female college students residing in Gangwon-do. It was conducted using ultrasound measurement of calcaneus bone density, anthropometric checkup and food-frequency questionnaires (FFQ) comprising 94 kinds of commonly consumed foods. Subjects were divided into two groups according to the T-score of bone density: a normal group (n = 113 persons, T-score ${\geq}1$) and a risk group (n = 47, T-score < 1.0). The average age of the subjects was 20.17 years and there was no significant difference between the two groups. Body weight and body fat percentage of the normal group were significantly higher than those of the risk group. The mean daily energy intake of the normal group was significantly higher than that of the risk group. Also, protein, fat, vitamin A, niacin, vitamin B6, folate, calcium, phosphorus, sodium, potassium, iron and zinc intake for the normal group were significantly higher than for the risk group. For the intake of the commonly consumed foods (or dishes) listed in FFQ, the mean daily intake amount of loaf bread, rice cake, potatoes, spicy beef soup, cucumber, seasoned spinach perilla leaves, crown daisy, stir-fried mushroom, sea mustard, beef rib, ham, chicken, mackerel, common squid, drink type curd yogurt, oriental melon and chocolate in the normal group was significantly higher than in the risk group. While, the mean daily intake of ramyun (instant noodle) and carbonated beverage by the normal group was significantly lower than that of the risk group. In conclusion, 20 something female college students showed a higher rate (26.9%) of the bone mineral density risk group (osteopenia or osteoporosis). For the risk group, the levels of nutrient and food intake were lower than in the normal group. Therefore, the bone density risk group needs to increase their nutrient intake and diet quality by increasing the intake of various foods. In addition, they should decrease the intake of foods, which are negative for skeletal health such as instant noodles and carbonated beverages.

• 한국식품과학회지
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• 제37권2호
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• pp.199-205
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• 2005

본 연구는 제빵 산업의 현장에서 제빵적성 향상을 위한 제빵법을 확립하기 위하여 백국균을 이용한 밀가루 코지, 유산균, 효모를 혼합 배양하여 seed mash를 만들어 그 특성을 분석하였다. 증류수 560mL와 코지 400g을 혼합하여 $25^{\circ}C$에서 72시간 배양한 seed mash의 효소 활성을 측정한 결과는 ${\alpha}-amylase$는 0.26 SKB/g, saccarifying amylase 활성이 36SP/g,그리고 산성 protease 활성은 648 HUT/g을 나타냈다. 코지, 유산균, 효모를 배양하여 제조한 seed mash의 pH는 코지와 효모 만을 배양하여 제조된 seed mash보다 다소 낮았다. 배양기간에 따른 환원당의 변화는 코지만을 이용한 seed mash의 경우 36시간까지 배양기간이 증가함에 따라 증가하였으며, 그 이후 감소하였다. 그러나 유산균과 효모를 첨가한 seed mash에서의 환원당은 배양 6시간까지 증가하다 그 이후 급격히 감소하였다. Seed mash에서 생성된 총산의 양은 10.4-12.25mg/mL로 유산균을 첨가시 다소 높았다. 코지, 효모, L. brevis로 배양한 seed mash에서 ethyl acetate, ethyl caprylate, isoamyl acetate, ethyl caprate, p-vinyl guaiacol 등의 향기 성분이 검출되었다.