• 한국동물위생학회지
• /
• 제21권3호
• /
• pp.301-311
• /
• 1998

This study was carried out species identification and antibiotics susceptibility of Staphylococci Isolated from bovine mastitic milk, chicks, Korean indigenous goats, pigs and mice in northern area of Kyungbuk. The result were summarized as follows ; A total of 71 Staphylococci were Isolated from bovine mastitic milk, chicken, pig, Korean indigenous goat and mouse. The results of identification of 71 Staphylococci revealed that S. aureus was most important pathogen in animals tested. Of 39 Staphylococci from bovine mastitic milk, 16 of 39 isolates (41%) were S. aureus and 9 of 39 isolates (23% ) were S. hyicus subsp chromogens. The results of susceptibility test to 16 antibiotics revealed that 91.5% of all isolates were resistant to more than 1 antibiotic and resistance to penicillin was most high (76.1%), All Isolates were susceptible to vancomycin.

• 한국동물위생학회지
• /
• 제21권3호
• /
• pp.255-260
• /
• 1998

Since bovine leukosis caused considerable economic loss to the dairy industry, seroepidemiologi-cal survey on bovine leukosis was carried out for the dairy herds in Kyunggi province. 1. When compared the results of immunodifussion test with those of enzyme-linked immunosorbent assay(ELISA) for 94 dairy herds sera, the relationship between the immunodifussion test and ELISA were showen high corresponding rate with sensitivity(97.5%) and specificity(92.6%). 2. In immunodiffusion test for bovine leukosis virus (BLV) antibody in 570 dairy cattle from 30 herds, mean positive rate for BLV antibody was 28.2%. The positive rate by districts were 16.5% in central, 35.4% in east, 17.3% in west, 29.1% in south, 31.6% in north, 43.7% in northeast. 3. When the results of serological studies was analyzed by age groups, the number of positive was increased gradually with the advanced in age of herds. The highest positive rate was found in the age over 6 years. 4. Of 30 dairy herds examined, 5 herds(16.7%) have no reactions against BLV antigen while 15 herds (50%) showed the range of 1∼5 positive cattle and 5 herds(16.7%), the rang of over 11 positive cattle.

• Asian-Australasian Journal of Animal Sciences
• /
• 제13권9호
• /
• pp.1323-1328
• /
• 2000

Bovine colostrum, which contains a large quantity of immunoglobulins, is indispensable for newborn animals. The establishment of a new procedure for preserving colostrum without losing the immunological activity is significant. We examined the effect of high hydrostatic pressure treatment (100~500 MPa) on the preservability and the immunochemical activity of bovine colostrum. When high hydrostatic pressure treatment was 300 MPa or more, the increase of the total viable count, coliforms and psychrotrophic gram-negative bacteria was suppressed effectively. In particular, the number of coliforms in colostrum treated at 300 MPa or more hardly increased for 35 days at $4{^{\circ}C}$. At 400 MPa or more, both gelling of the colostrum and denaturation of immunoglobulins were observed. However, if the pressure was 300 MPa, immunoglobulins were scarcely influenced and the neutralizing titers against the bovine coronavirus did not decrease. Therefore, it was suggested that 300 MPa was the best pressure for good preservability of colostrum without reducing the immunochemical response.

• 한국가축번식학회지
• /
• 제17권1호
• /
• pp.69-74
• /
• 1993

This study was investigated to examine the effect of conditioned medium from bovine oviductal cell(BOEC) in the co-culture system with BOEC on in vitro development of in vitro produced bovine embryos. Oocyte-cumulus complexes were cultured for 24 hrs in TCM-199 supplemented with 10% fetal calf serum, 1$\mu\textrm{g}$/ml FSH and 21U hCG, 1$\mu\textrm{g}$/ml oestradiol-17$\beta$ at 39$^{\circ}C$ under 5% CO2 in air. In vitro fertilization was performed with epididymal sperm and heparin (10$\mu\textrm{g}$/ml, 15min.) or caffeine(2.5mM)-treated spermatozoa. Oocytes were incubated with 1$\times$106 spermatozoa/ml for 18 hrs and then cultured in various culture system for 7 days. The development rates of 16-cell or blastocyst stages were recorded on 4, 7 days, respectively, after incubating. The proportions ofembryonic development into molulae and blastocysts were higher in cumulus cell co-culture(23.4%) and BOEC co-culture(34.3%) than in M199-FCS(6.1%). Similarily, the development rates into molulae and blastocysts were significantly higher in BOEC-conditioned medium than those in M199-FCS. Therefore, it is suggested that BOEC co-culture and BOEC conditioned medium increase significantly the development of in vitro produced bovine embryos in in vitro system.

• 한국가축번식학회지
• /
• 제22권1호
• /
• pp.95-99
• /
• 1998

Three experiments were conducted with follicular oocytes, to compare some somatic cells, growth factors and media for in vitro maturation of bovine oocytes. In the first experiment, the type of somatic cells had no effects on in vitro maturation of bovine follicular ooctyes. In the second experiment, oocytes were matured in TCM199 su, pp.emented with growth factors on IVM of bovine follicular oocytes, then all were co-cultured with cumulus cells. The proportion of used oocytes that developed to expanding blastocysts was 22.2%, 20.2%, 17.7%, 22.2%, 24.4% and 20.2% after maturation in TCM199 su, pp.emented with control, insulin, IGF-I, IGF-Ⅱ, FGF and EGF, respectively. In the third experiment, oocytes were matured in BO, Ham's F10 and TCM199, then all were fertilized in BO, and embryos cultured in BO, Ham's F10 and TCM199, respectively. Cleavage rates in BO were 90%, had higher than in Ham's F10(80%) or in TCM199(64%). But production of expanding blastocysts in TCM199(21%) or Ham's F10(20.6%), had higher than in BO(4.6%).

• 한국임상수의학회지
• /
• 제19권2호
• /
• pp.115-120
• /
• 2002

To clarify the therapeutic effect of aquapuncture therapy on bovine foot rot, the experimental cattle were divided into control (conventional treatment), aquapuncture and combined treatment groups. Each group was composed of 6 Holstein cows with bovine foot rot. In the present study, the change in grade of lameness, total leukocyte counts, N/L. ratio, mean recovery rates (days) and histopathological changes of interdigital tissue before and after treatment were evaluated. The grade of lameness was decreased by treatment in each group, with the decreasing in order of combined > aquapuncture > control group. In change of total peripheral blood leukocyte counts, the tendency of decrease was found. Significances were detected on 9th day (P < 0.05) in control, on 6th day (P < 0.01) in aquapuncture group, and on 3rd (P < 0.05) and 6th day (P < 0.05) in combined group, respectively. In addition, significances were detected on 3rd and 6th day in aquapuncture, and on 3rd day in combined group by comparison with that of control, respectively. N/L ratio was decreased by therapy in each group with the decreasing in order of combined > aquapuncture ) control group. Significances were detected on 6th day (P < 0.01) between aquapuncture and combined groups. The mean recovery rates(days) were rapid with the decreasing in order of combined > acuapuncture > control group. In histopathological findings of interdigital tissue, the findings of inflammation and hemorrhage were not observed after treatment in each group. In conclusion, it was thought that aquapuncture and combined therapy was very effective on treatment of bovine foot rot.

• 대한바이러스학회지
• /
• 제30권1호
• /
• pp.1-10
• /
• 2000

The nonstructural protein NSP4, encoded by gene 10 of rotavirus, has been shown to playa role in viral assembly and known to be an enterotoxin, causing diarrhea in mouse pups. NSP4 gene was cloned from CBNU-2 (virulent bovine rotavirus/diarrheic fecal sample) and CBNU-1 (cell-culture adapted bovine rotavirus/isolated from CBNU-2 and 75 times passaged on MA104 cells), respectively, by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced and compared. The sequence data indicated that the NSP4 genes of bovine rotavirus (BRV) were 751 bases in length and encoded one open reading frame of 175 amino acids beginning at base 42 and terminating at base 569. Differences in nucleotide sequence between CBNU-2 and CBNU-1 were observed at 6 positions (base 274, 296, 391, 394, 396 and 579). NSP4 gene of BRV exhibited a high degree of nucleotide (90% and 94%) and amino acid sequence (91% and 97%) homology with those of SA11 and UK but a low degree of nucleotide (77% and 79%) and amino acids sequence (81% and 85%) homology with those of Wa and OSU.

• 한국수정란이식학회지
• /
• 제28권1호
• /
• pp.57-61
• /
• 2013

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.

• 대한수의학회지
• /
• 제35권1호
• /
• pp.67-73
• /
• 1995

아무르장지뱀(Takydromus amurensis)의 위장관 점막에 분포하는 내분비세포의 분포와 출현빈도 및 세포의 종류를 알아보기 위하여 몇가지 도은법 및 면역조직화학적 방법으로 관찰하였다. Grimelius법에만 염색된 은호성세포(argyrophil cell)는 유문부에 다수로 십이지장에 중등도로 국한되어 출현하였다. 한편 bovine CG면역반응세포는 유문부에서 최고의 빈도로 전장관에 걸쳐 분포하였다. BPP면역반응세포는 소장부위에서만 동정되었다. 따라서 아무르장지뱀의 위장관 내분비세포에서 은호성세포와 bovine CG면역반응세포는 분포와 출현빈도에서 일치하지 않았다.

• 한국가축번식학회지
• /
• 제22권3호
• /
• pp.213-219
• /
• 1998

This study was carried out to investigate on the improvement of fertilizing ability of in vitro matured oocytes from sperm density, motility and polyvinylpyrrolidine (PVP) concentration, by intracytoplasmic sperm injection(ICSI) into the bovine oocytes. 1. The in vitro fertilization and cleavage rates of oocytes from 1.0, 2.0, 3.0, 5.0 ($\times$106/ml) sperm concentration by IVF and ICSI of bovine oocytes were 45.0%~65.0%, 65.0%~90.0% and 10.0%~30.0%, 35.0%~70.0%, respectively. 2. The in vitro fertilization and cleavage rates of oocytes from 20, 40, 60, 80% of sperm motility by IVF and ICSI of bovine oocytes were 47.8%~75.0%, 78.3%~90.0% and 8.7%~25.0%, 34.8%~70.0%, respectively. 3. The in vitro fertilization and cleavage rates of oocytes from 0.01, 0.02, 0.03, 0.05% of PVP concentration by microinjection of single into the bovine oocytes were 72.7%, 90.9%, 83.3%, 76.9% and 45.5%, 72.7%, 58.3%, 61.5%, respectively and these values of 0.02% addition of PVP were higher than other concentrations of PVP. 4. The in vitro fertilization and developmental rates of oocytes by IVF and ICSI methods were 63.3%~64.6%, 26.7%~29.2% and 88.2%, 47.1%, respectively. This ICSI method was improved high fertilization rates of bovined oocytes.