• Title/Summary/Keyword: bovine amniotic membrane transplantation

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Bovine Amniotic Membrane Transplantation for the Treatment of Descemetocele in a Dog (개에서 데스메막류 치료를 위한 양막이식의 적용)

  • Kang, Myung-Gon;Choi, Yong-Hoon;Kim, Jun-Young;Jeong, Soon-Wuk
    • Journal of Veterinary Clinics
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    • v.23 no.3
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    • pp.334-336
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    • 2006
  • An 1.6-year-old female Shih Tzu was presented with corneal perforation, descemetocele, and deep corneal ulcer in the left eye. A bovine amniotic membrane graft which preserved in lyophylized dry form and a third eyelid flap were applied. After 14 days, the cornea was fluorescein-negative. At day 42 postoperatively, only a moderate scar at the corneal center was showed. At the 10-month follow-up, there was clear cornea with a mild scar.

Inactivation of Infectious Microorganisms by Disinfection and Sterilization Processes for Human Amniotic Membrane Grafts (이식을 위한 사람 양막의 소독 및 멸균공정에 의한 감염성 위해인자 불활화 효과)

  • Bae, Jung-Eun;Kim, Chan-Kyung;Kim, In-Seop
    • Korean Journal of Microbiology
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    • v.45 no.4
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    • pp.346-353
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    • 2009
  • Viral, bacterial, and fungal infection can be transmitted from donor to recipient via transplantation of human amniotic membrane. Therefore human amniotic membrane for transplantation should be disinfected and sterilized before use. The purpose of this study was to examine the efficacy of the disinfection process and sterilization processes used at human tissue bank in the inactivation of viruses, bacteria, and fungi. A variety of experimental model viruses, bacteria, and fungus for human pathogens, including the human immunodeficiency virus type 1 (HIV-1), bovine herpes virus (BHV), bovine viral diarrhoea virus (BVDV), hepatitis A virus (HAV), porcine parvovirus (PPV), Escherichia coli, Bacillus subtilis, and Candida albicans were all selected for this study. Enveloped viruses such as HIV-1, BHV, and BVDV were effectively inactivated to undetectable levels by 70% ethanol treatment, gamma irradiation process, and ethylene oxide (EO) gas sterilization process. Also non-enveloped viruses such as HAV and PPV were effectively inactivated to undetectable levels by gamma irradiation and EO gas treatment. However HAV and PPV showed high resistance to 70% ethanol treatment. E. coli and C. albicans were effectively inactivated to undetectable levels by 70% ethanol treatment, gamma irradiation process, and EO gas treatment. Also B. subtilis was effectively inactivated to undetectable levels by gamma irradiation process and EO gas treatment. However it showed high resistance to 70% ethanol treatment.