• Horticultural Science & Technology
• /
• v.32 no.1
• /
• pp.26-32
• /
• 2014

We investigate the effect of post-conditioning periods and seed orientation on the vigor of cucurbit seeds with dry heat treatment (DHT). All the dry-heat treated seeds exhibited varying degree of seed vigor decreases. In general, pumpkin seeds showed less vigor decreases than the bottle gourd seeds. When the dry heat treated seeds were germinated after post-conditioning for 0, 30, and 120 days, the percentage of germination was enhanced by increasing the period of post-conditioning and the efficiency of post-conditioning differed by crop and cultivar. In both bottle gourd and pumpkin, the vigor of seeds placed in vertically upward and horizontal orientations was higher than that of the seeds placed in the vertically downward orientation. The results suggested that the vigor of dry-heat treated seeds could be improved by applying the proper post-conditioning and seed orientation.

• Journal of Bio-Environment Control
• /
• v.29 no.1
• /
• pp.96-102
• /
• 2020

Fusarium wilt caused by Fusarium oxysporum is a devastating disease limiting production of watermelon in Korea. The best way to control diseases is to use resistant gourd rootstock on watermelon. This study was conducted to establish an efficient screening method for resistant bottle gourd to Fusarium oxysporum f. sp. lagenaria. To develop an efficient inoculation method, incubation temperature after inoculation (15, 20, 25, and 30℃), inoculum concentration (1 × 10⁵, 5 × 10⁵, 1 × 10⁶, and 5 × 10⁶ conidia·mL^-1), and growth stages of seedlings (7, 10, 13, and 16 days) was investigated. Disease development of Fusarium wilt of bottle gourd was little affected by differences in incubation temperature and growth stages of seedlings. But resistant lines were more susceptible and appeared more severe symptoms at the higher inoculation level. Taken together, we suggest that an effective screening method for resistant gourd plant to Fusarium wilt is to dip the roots of 10-day old seedlings in spore suspension of 1 × 10⁵ - 1 × 10⁶ conidia·mL^-1, for 30 min, to transplant the seedlings into a non-infected soil, and then to incubate the inoculated plants in a growth room at 25℃ for 3 weeks to develop Fusarium wilt.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2003.02a
• /
• pp.366-371
• /
• 2003

박 종자의 경우 CGMMV(Cucumber Green Mosaic Mottle Virus:오이녹반모자이크바이러스)에 감염되면 성체의 형태에서 농약으로 효과적인 방제가 불가능하다고 알려져 있다. 최근 국내외 종자회사에서 이병 된 종자를 판매하여 농가와 종자회사에 큰 타격을 입힌 것이 계기가 되어 바이러스 방제에 대한 효과적인 방제방법의 연구에 관심이 집중되고 있다. (중략)

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2000.10a
• /
• pp.63.2-63
• /
• 2000

• Journal of Plant Biotechnology
• /
• v.33 no.1
• /
• pp.33-37
• /
• 2006

Bottle gourd (Lagenaria siceraria Standl.) has been used as a rootstock for the watermelon cultivation because of better growth ability at low temperature and avoidance from contamination of the soil disease. Since the genetic source for the elite rootstock is limited in nature, the genetic engineering method is inevitable to develop new lines especially to obtain the functionally important or multi-disease resistant bottle gourd. Recently, our lab has set up a successful system to transform the bottle gourd. in order to monitor the transformation process, GFP gene is used. Cotyledons of the inbred line 9005, 9006 and G5 were used to induce the shoot under the selection media with MS + 30 g/L sucrose + 3.0 mg/L BAP + 100 mg/L kanamycin + 500 mg/L cefotaxime + 0.5 mg/L $AgNO_3$, pH 5.8. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. The shoot was incubated in the rooting media with 1/2 MS + 30 g/L sucrose + 0.1 mg/L IAA + 50 mg/L kanamycin + 500 mg/L cefotaxime, pH 5.8 and moved to pot for acclimation. Although the shoot development rate was depended on the genotype, the G5 was the best line to be transformed. Monitoring GFP expression from the young shoot under microscope could make the selection much easier to distinguish the transformed shoot from the non-transformed shoots.

• Journal of Life Science
• /
• v.11 no.2
• /
• pp.166-172
• /
• 2001

Various types of the seeding of bottle gourd widely used as a rootstock of watermelon has been required to satisfy the farmers need. The study was done to determine the effect of light quality of blue, red and far-red lights treated with light emitting diodes on growth and morphology of bottle goured seeding and chlorophyll content of its cotyledons. The lights were treated in the growth chamber for 7 days to the seeding elapsed 8 days after sowing under natural condition, and 64 hole trays with commercial bedsoil. Plant height, length and diameter of hypocotyl, leaf area of cotyledon and first true leaf, its leaf length, number of true leaves, fraction and total dry weight were measured. Red light shortened and slenderized the hypocotyl, which lengthened by far-red light and thickened by blue light. Plant height was declined in order of far-red light treatment, blue and red lights mainly due to difference of hypocotyl length, Area and length of the first true leaf became smaller and shorter under far-red light than under the other lights. However, blue light increased leaf area of cotyledons. Two cultivars cv. Yongjadaemok and cv. Kunghap had different response to the light treatments in total seedling dry weight(dw); far-red and red light treatments showed the greatest and the least dw of hypocotyl, respectively, while blue and red lights did the greatest dw of the other organs. Among the ratio of each organ dw to total dw, those of hypocotyl and true leaves were different between the light treatments; the highest ratio of hypocotyl dw to total dw was observed in far-red light treatment but the lowest was in red light treatment. Those of the true leaves were the lowest in far-red light and similar response in blue or red light treatment. Chlorophyll content of cotyledons was decreased in order of red light treatment, blue and far-red lights, meaning that short period light treatment may influence photosynthesis of seeding and afterward its growth.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 2000.10b
• /
• pp.19-22
• /
• 2000

접목을 실시할 때 접수와 대목의 적정한 생육은 접목을 용이하게 할 뿐만 아니라 활착 후 접목묘의 묘소질 지속에도 커다란 영향을 미칠 수 있다. 자연광 조건에서 육묘를 실시하면 묘의 생육이 불균일하여 묘소질이 일정치 않게 된다. 그러므로 인공광을 이용한 폐쇄된 환경 조건에서 육묘가 바람직하나, 대목용 박에 대한 적정 광합성 유효광량자속 또는 광주기가 제시되지 않고 있다. 본 연구의 목적은 대목용 박의 초기 생육에 미치는 광합성 유효광량자속과 광주기의 영향을 구명하는 데 있다. (중략)

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2007.11a
• /
• pp.224.2-224.2
• /
• 2007

• The Plant Pathology Journal
• /
• v.17 no.2
• /
• pp.106-109
• /
• 2001

A multi-rapid immunofilter paper assay (multi-RIPA) system was prepared for simultaneous diagnosis of three Tobamoviruses, Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) in cucurbitaceous crops. Each of these viruses was specifically detected by the multi-RIPA from cucumber, watermelon, zucchini, and bottle gourd inoculated with the three Tobamoviruses singly or in combination. The three viruses could infect cucumber, watermelon, and bottle gourd ; however, CGMMV could not infect zucchini as the latex-coated CGMMV antibody showed a negative reaction in the multi-RIPA of the virus-infected plant extract. When the minimum detection level of multi-RIPA was compared with that of double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) using CGMMV, the latter was 10 times more sensitive than the former. The detection limit of the multi-RIPA for the purified CGMMV was 50 ng/ml. In a survey of the threeviruses in cucurbits growing in commercial fields in 1999 and 2000, CGMMV was detected in watermelon and cucumber, and ZGMMV was detected only in zucchini growing in plastic houses at the suburbs of Chonju, Korea. However, KGMMV was not found in the commercially growing cucurbit crops in our study, The results suggest that the multi-RIPA can be a simple, rapid, specific and convenient tool to detect simultaneously the Tobamoviruses.

• Journal of the Korean Society of Costume
• /
• v.56 no.7 s.106
• /
• pp.54-68
• /
• 2006

This study examined similarity between white porcelain models and dress silhouette of women painted in genre painting in late Joseon period, and found that gourd bottles that are one of main porcelain styles are very similar with dress silhouette of women presented in genre painting in Joseon period; that is, bottle necks of gourd bottles and women's waist, voluminous bottles and erotically full hips, the length of skirts which showed underwear dress and height of porcelain's bottom, waist panel winding slim waist and wide edge of porcelain's mouth. As images are personal mental process of potters who were not socially controlled, they used dress as an indirect means of women's bodies for the subjects of images that are obtained through direct and indirect experiences of the persons who perceive. It indicates that they intended to make their ceramic works express women's dressing style through images of porcelain's models. Such images were sensual, but not superficial. It is suggested that they were influenced by the society which considered ethical experimental mind and practicality of the governing class of that time who enjoyed it metaphorically, but not impudent.